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Animal Sciences

2014

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Morphological And Morphometrical Characteristics Of The Interdigital Gland In Kivircik Sheep, Yasi̇n Demi̇raslan, Yalçin Akbulut, Turgay Deprem, Ebru Karadağ Sari, Kadi̇r Aslan Jan 2014

Morphological And Morphometrical Characteristics Of The Interdigital Gland In Kivircik Sheep, Yasi̇n Demi̇raslan, Yalçin Akbulut, Turgay Deprem, Ebru Karadağ Sari, Kadi̇r Aslan

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the morphological and morphometric characteristics of the interdigital gland in Kivircik sheep. The interdigital glands in the forefeet and hind feet of the 16 male Kivircik sheep were examined in terms of morphometric and morphological characteristics. Four lengths (length of duct and corpus, diameter of duct and corpus) and 2 angles (angle of internal and external flexure) were measured in the interdigital glands located between 2 digits after dissection. The interdigital gland contained orifice, excretory duct, flexure, and corpus sections. Lengths and widths of corpus and excretory ducts of the interdigital gland …


Impact Of The Depletion Of High-Abundance Proteins In Blood Plasma/Serum On The Proteome Of These Media In Growing Farm Animals, Adam Lepczynski, Alicja Dratwa-Chalupnik, Agnieszka Herosimczyk, Katarzyna Staszak, Arleta Majewska, Malgorzata Ozgo Jan 2014

Impact Of The Depletion Of High-Abundance Proteins In Blood Plasma/Serum On The Proteome Of These Media In Growing Farm Animals, Adam Lepczynski, Alicja Dratwa-Chalupnik, Agnieszka Herosimczyk, Katarzyna Staszak, Arleta Majewska, Malgorzata Ozgo

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE). Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma (+26.46%) and in piglet serum (+49.40%). Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. …


Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak Jan 2014

Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to compare the effectiveness of cefuroxime, herd-specific autovaccine, and cefuroxime/herd-specific autovaccine treatments in eliminating S. aureus from the milk of 45 cows with subclinical mastitis. Prior to this study, cows with S. aureus mastitis were divided into 3 groups of 15 cows each. Group 1 received cefuroxime, group 2 received the autovaccine, and group 3 received cefuroxime and the autovaccine. The antibiotic was infused into the teat canal, whereas the autovaccine was administrated in the upper udder lymph node region. It was revealed that after day 35 of the treatment S. aureus was …


Phylogenetic Analysis Of Ns Gene Of Avian Influenza Viruses (H9n2) Isolated From Chicken In Iran During 2007-2011, Hassan Norouzian, Hessam Emadi Chashmi, Ziba Nazari, Mehdi Vasfi Marandi Jan 2014

Phylogenetic Analysis Of Ns Gene Of Avian Influenza Viruses (H9n2) Isolated From Chicken In Iran During 2007-2011, Hassan Norouzian, Hessam Emadi Chashmi, Ziba Nazari, Mehdi Vasfi Marandi

Turkish Journal of Veterinary & Animal Sciences

To determine sequence characteristics and phylogenetic relationships of the nonstructural (NS) gene, the NS genes of 9 avian influenza (AI) viruses were sequenced and compared with other Iranian AI H9N2 viruses available in GenBank. The similarity among the earliest Iranian H9N2 isolate (A/chicken/Iran/ZMT-101/1998) and recent (2007–2011) studied isolates was low (94%). Some of the studied viruses revealed substitutions in RNA-binding and effector domains as well as antigenic regions of the NS protein. Recently studied isolates have a high similarity (97.4%-98.7%) with some Pakistani isolates (like A/chicken/Sawabi/NARC-2434/2006) in the NS gene. In the phylogenetic analysis, recently studied isolates composed a cluster …


Detection Of Sensitive And Mutant Ruminal Bacteria Isolates From Sheep, Cattle, And Buffalo Using 14 Therapeutic Antibiotics, Abdelfattah Zeidan Mohamed Salem, Roberto Montes De Oca Jimenez, Maria Andrea Cerrillo-Soto, Ahmed Eid Kholif, Mohamed Zidan Mohamed Salem, Mona Mohamed Mohamed Yasseen Elghandour Jan 2014

Detection Of Sensitive And Mutant Ruminal Bacteria Isolates From Sheep, Cattle, And Buffalo Using 14 Therapeutic Antibiotics, Abdelfattah Zeidan Mohamed Salem, Roberto Montes De Oca Jimenez, Maria Andrea Cerrillo-Soto, Ahmed Eid Kholif, Mohamed Zidan Mohamed Salem, Mona Mohamed Mohamed Yasseen Elghandour

Turkish Journal of Veterinary & Animal Sciences

In the present study, sensitive and mutant colonies of some ruminal bacterial species isolated from sheep, cattle, and buffalo were detected. We counted and considered \"mutant colonies\" the bacterial colonies grown in the clear inhibition zone in the Kirby-Bauer disk diffusion susceptibility test. Detected mutant colonies were higher in buffalo than in cattle and sheep. Duricef and metronidazole caused no mutations in any species. The others formed mutant colonies, where roxithromycin = polymyxin = chloramphenicol = gentamicin < erythromycin < vancomycin < piperacillin = cefotaxime < streptomycin < cefoperazone < ciprofloxacin < amikacin. Sheep had the highest number of sensitive isolates, and the number of sensitive isolates was dramatically lower in cattle and buffalo. There were no sensitive isolates with the antibiotic metronidazole, and there was a low number of sensitive isolates with duricef. The other antibiotics had more sensitive isolates (gentamicin = ciprofloxacin = amikacin > streptomycin = piperacillin > erythromycin > vancomycin = cefoperazone = cefotaxime > roxithromycin > polymyxin > chloramphenicol). The number of sensitive isolates of the different ruminant species for …


The Midgut Bacterial Flora Of The Hard Tick Hyalomma Anatolicum (Acari: Ixodidae) From South India As Determined By Molecular Analyses, Sankarappan Anbalagan, Vimalanathan Arun Prasanna, Mani Kannan, Muthukalingan Krishnan Jan 2014

The Midgut Bacterial Flora Of The Hard Tick Hyalomma Anatolicum (Acari: Ixodidae) From South India As Determined By Molecular Analyses, Sankarappan Anbalagan, Vimalanathan Arun Prasanna, Mani Kannan, Muthukalingan Krishnan

Turkish Journal of Veterinary & Animal Sciences

The midgut bacterial flora of post-blood meal and starved or digested blood meal of hard ticks (Hyalomma anatolicum) from goats were analyzed using BOX PCR, ERIC PCR and 16S rDNA gene sequencing. BOX PCR and ERIC PCR showed dissimilar band patterns between microbial isolates from the midgut of the ticks. In total, 9 bacterial species belonging to 2 genera (Bacillus and Staphylococcus) were identified in the midgut of both types (post-blood meal and digested blood meal) of tick by using 16S rDNA gene sequencing. The majority of the midgut bacterial species were Staphylococcus sp. (90%) and there was a high …


Effects Of Supplementation Of Inorganic Sulfur On Some Biochemical Parameters In Angora Goat’S Diet Containing High Nitrate Levels, Mehmet Özdemi̇r, Abdullah Eryavuz, Gülcan Avci, Yavuz Osman Bi̇rdane, İsmai̇l Küçükkurt Jan 2014

Effects Of Supplementation Of Inorganic Sulfur On Some Biochemical Parameters In Angora Goat’S Diet Containing High Nitrate Levels, Mehmet Özdemi̇r, Abdullah Eryavuz, Gülcan Avci, Yavuz Osman Bi̇rdane, İsmai̇l Küçükkurt

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the effect of supplementation of sulfur to a diet containing sodium nitrate at a level that would cause chronic nitrate poisoning on some rumen and biochemical parameters in Angora goats. A total of 18 male Angora goats were used. The animals in the control group were fed the basal diet, while the animals were fed experimental concentrate containing 1500 mg/kg sodium nitrate in the nitrate group (N) and experimental concentrate containing 1500 mg/kg sodium nitrate plus 1.8% sodium sulfate in the nitrate-sulfur group (NS) throughout the study, which lasted for 6 months. …


Clinical Presentation And Ultrasonographic Findings In Buffaloes With Congestive Heart Failure, Hussein Awad Hussein, Rudolf Staufenbiel Jan 2014

Clinical Presentation And Ultrasonographic Findings In Buffaloes With Congestive Heart Failure, Hussein Awad Hussein, Rudolf Staufenbiel

Turkish Journal of Veterinary & Animal Sciences

The objective of this retrospective study was to describe the clinical presentation and ultrasonographic findings in water buffaloes suffering from congestive heart failure. Fifty-three buffaloes with congestive heart failure, which was confirmed postmortem, were included. The final diagnoses were traumatic pericarditis (n = 38) and vegetative endocarditis (n = 15). Ten healthy buffaloes were enrolled in this study as controls. The main clinical presentations were those related to right-sided congestive heart failure including peripheral edema, venous distension and jugular pulsation, and a sharp drop in milk yield. Only 5 buffaloes exhibited signs of left-sided heart failure. Ultrasonographic examinations showed cardiac …


Developments In The Performance Of Brown Egg Layer Parental Stocks For Superior Hybrid, Hüseyi̇n Göger, Şahnur Erdoğan Demi̇rtaş, Şermi̇n Yurtoğullari Jan 2014

Developments In The Performance Of Brown Egg Layer Parental Stocks For Superior Hybrid, Hüseyi̇n Göger, Şahnur Erdoğan Demi̇rtaş, Şermi̇n Yurtoğullari

Turkish Journal of Veterinary & Animal Sciences

This study examines the effect of grandparent, parent, and hybrid brown chickens, selected from 3 generations of pure lines, on hybrid egg-laying performance. The study was conducted at the Poultry Research Station of Ankara. In the first stage, 4 grandparent lines were derived from 2 pure line base populations. Grandparent lines included 2 male lines (low body weight and high egg weight) and 2 female lines (low body weight and high egg production). In the second stage, grandparent male and female lines were reciprocally crossed to produce 4 parent lines. In the third stage, male and female parental lines were …


Genetic Parameter Estimates For Selected Type Traits And Milk Production Traits Of Holstein Cattle In Southern China, Songbai Liu, Huize Tan, Lu Yang, Jianming Yi Jan 2014

Genetic Parameter Estimates For Selected Type Traits And Milk Production Traits Of Holstein Cattle In Southern China, Songbai Liu, Huize Tan, Lu Yang, Jianming Yi

Turkish Journal of Veterinary & Animal Sciences

Phenotypic and genetic parameters among udder traits (udder depth, UD; median suspensory line, MS; fore udder attachment, FUA; rear udder height, RUH; rear udder width, RUW), final score (FS), and milk production traits (305-day milk yield, 305 DM; 305-day milk-fat yield, 305 DF; 305-day milk-protein yield, 305 DP) were estimated using a derivative-free restricted maximum likelihood procedure with an animal model. The results showed that heritability estimates for selected traits ranged from 0.11 to 0.24. Strong positive genetic correlations were observed for MS, RUH, and RUW with FS (0.42-0.72). A positive genetic relationship was also estimated between MS and 305 …


Effect Of Sodium Bicarbonate Supplementation And 2 Different Ambient Temperatures On Growth Performance And Carcass Characteristics Of Lambs Fed Concentrate Diets, Demba Boi Jallow, Liang Chou Hsia Jan 2014

Effect Of Sodium Bicarbonate Supplementation And 2 Different Ambient Temperatures On Growth Performance And Carcass Characteristics Of Lambs Fed Concentrate Diets, Demba Boi Jallow, Liang Chou Hsia

Turkish Journal of Veterinary & Animal Sciences

Twenty male Barbados lambs (3 months old) were used to evaluate the effects of sodium bicarbonate (NaHCO_3) supplementation on growth performance and carcass characteristics of lambs fed concentrate diets in controlled-environment chambers set at 20 °C and 30 °C. In the 35-week-long growth trial, the animals were divided into 4 groups (n = 5) and fed 2 dietary treatments: a basal diet (35:65 roughage and concentrate) or a basal diet supplemented with 4% NaHCO_3. Twelve lambs randomly selected from this trial were arranged in a 2 × 2 factorial design for a 10-day-long slaughter and carcass evaluation experiment. Lambs in …


A Survey Of Selected Minerals In Ready-To-Eat Pastırma Types From Different Regions Of Turkey Using-Icp/Oes, Muhammet İrfan Aksu, Ebru Erdemi̇r Jan 2014

A Survey Of Selected Minerals In Ready-To-Eat Pastırma Types From Different Regions Of Turkey Using-Icp/Oes, Muhammet İrfan Aksu, Ebru Erdemi̇r

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the content of selected minerals (Ca, K, Na, Mg, S, P, Pb, Zn, Mn, Fe, and Ni) and moisture values in types of pastırma (sırt, kuşgömü, bohça, and şekerpare), a dry-cured meat product. Forty-four samples of pastırma were collected from Turkey and analyzed using inductively coupled plasma-optical emission spectrometry (ICP/OES) for quantitative determination. Na content was the highest out of all the other minerals in all the pastırma types (31.9-47.7 g kg^{-1} dry weight). The determined mean ranges of heavy metals were 2.6 ± 0.5 to 3.6 ± 0.8 Pb, 245.3 ± …


Co-Infection Of Water Buffaloes In Punjab, Pakistan, With Neospora Caninum And Brucella Abortus, Amar Nasir, Muhammad Ashraf, Abdul Shakoor, Muhammad Adil, Tariq Abbas, Muhammad Kashif, Muhammad Younus Rana, Michael Philipp Reichel Jan 2014

Co-Infection Of Water Buffaloes In Punjab, Pakistan, With Neospora Caninum And Brucella Abortus, Amar Nasir, Muhammad Ashraf, Abdul Shakoor, Muhammad Adil, Tariq Abbas, Muhammad Kashif, Muhammad Younus Rana, Michael Philipp Reichel

Turkish Journal of Veterinary & Animal Sciences

Neosporosis and brucellosis are 2 well-known diseases causing bovine abortion. This study determined co-infection of Neospora caninum and Brucella abortus in buffaloes for the first time in Pakistan. A monoclonal antibody based competitive ELISA was used to test buffalo sera (n = 312) for N. caninum specific antibodies, while rose bengal plate test assessed B. abortus antibodies. N. caninum and B. abortus prevalence was 42.8% (±6.7, 95% C.I.) and 12.2% (±3.6, 95% C.I.), respectively. Buffaloes >3 years old exhibited higher prevalence than younger ones. Co-infection was particularly significant as 13.2% of Neospora infected buffaloes were also Brucella infected with an …


Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos Jan 2014

Recent Advances In The Immunology And Uterine Microbiology Of Healthy Cows And Cows That Develop Uterine Disease, Klibs N. Galvao, Jose Eduardo P. Santos

Turkish Journal of Veterinary & Animal Sciences

Uterine diseases are highly prevalent in dairy cows. Causes of uterine diseases are multifactorial. There is good evidence for the susceptibility of the host and for the role of pathogenic bacteria, and less evidence for the effect of the environment. Uterine and leukocyte immune response is impaired early postpartum in cows that develop uterine disease. The decrease in immune function is associated with a decrease in calcium postpartum and an increase in NEFA and BHBA. Both endometrial cells and granulosal cells possess toll-like receptors (TLR) that can recognize and mount an inflammatory response to pathogen-associated molecular patterns (PAMPs) such as …


Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs Jan 2014

Maternal Embryokines That Regulate Development Of The Bovine Preimplantation Embryo, Peter James Hansen, Anna Carolina Denicol, Kyle Bradley Dobbs

Turkish Journal of Veterinary & Animal Sciences

Proper embryonic development depends upon regulatory signals from the reproductive tract called embryokines. This review uses the cow as a model to understand the developmental processes controlled by embryokines. The focus is on 3 embryokines that have been shown to increase competence of embryos to survive after transfer to recipients: CSF2, IGF1, and DKK1. Together, these molecules regulate key events required for development of the embryo to the blastocyst stage including proliferation (enhanced by IGF1), development of the inner cell mass (regulated by CSF2), control of differentiation (modified by DKK1), and inhibition of apoptosis and stress-mediated developmental arrest (IGF1 and …


The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao Jan 2014

The Micrornas Important For Ovarian And Early Embryonic Development In Cattle, Lei Wang, Jianbo Yao

Turkish Journal of Veterinary & Animal Sciences

Recent progress in genomic analysis and other biochemical methods has led to the discovery of a large population of microRNAs (miRNAs), which have been demonstrated to play important roles in diseases and a wide range of developmental processes. Characterization of miRNA expression profiles in different stages of ovarian follicular development and early embryogenesis has suggested the potential roles of miRNAs in follicular development, maturation of oocytes, and preimplantation embryonic development. This review focuses on the current studies of miRNAs involved in ovarian and early embryonic development in cattle.


Usefulness Of Short Sequence Repeat Markers In Goat Genetic Diversity Studies On The Asian And African Continents, Faiz M.M.T. Marikar, Muneeb M. Mustafa Jan 2014

Usefulness Of Short Sequence Repeat Markers In Goat Genetic Diversity Studies On The Asian And African Continents, Faiz M.M.T. Marikar, Muneeb M. Mustafa

Turkish Journal of Veterinary & Animal Sciences

Goat genetic diversity studies are very important since extinction of germ plasma is increasing very rapidly. The African and Asian continents are the hotspots for indigenous animals with fewer genetic manipulations. Conservation studies using molecular markers have the capability to validate the real status of the animals. The use of molecular markers has revolutionized studies of genetic diversity. Even though a number of markers are used for these types of studies, short sequence repeats are in the forefront due to their superior features such as high variability, high mutation rate, large number, distribution throughout the genome, codominant inheritance, and neutrality …


Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor Jan 2014

Polledness Intersex Syndrome In Goats – Molecular And Histological Aspects, Iwona Szatkowska, Daniel Zaborski, Witold Stanislaw Proskura, Sara Tabor

Turkish Journal of Veterinary & Animal Sciences

Polledness intersex syndrome (PIS) is a phenomenon associated with 2 distinct conditions occurring in goats, namely polledness and intersexuality. The first is a trait exhibiting complete dominance, whereas the latter is recessive. The extreme case of intersexualism is complete sex reversal, which is observed only in females (60,XX). The trait PIS is inherited in a recessive manner and caused by deletion of 11.7 kbp DNA on chromosome 1q43. Female goats with PIS exhibit a broad range of developmental abnormalities of the sexual system, from only subtle changes to complete sex reversal. The phenotypic diversity in goats with PIS is most …


Application Of Dietary Fish Oil In Dairy Cow Reproduction, Anna Zwyrzykowska, Robert Kupczynski Jan 2014

Application Of Dietary Fish Oil In Dairy Cow Reproduction, Anna Zwyrzykowska, Robert Kupczynski

Turkish Journal of Veterinary & Animal Sciences

Feed fats are commonly used as a component of high-yielding cow diet rations. Many studies have been conducted on the effects of fat supplementation on metabolism, milk composition, and improvements in dairy cow reproduction. The positive effects on fat (vegetable oil) and energy supplementation on improvements in reproduction are well documented. Consumption of polyunsaturated fatty acids (PUFAs), including n-3 acids, in appropriate feed doses improves fertility of dairy cows. A fat source with high levels of acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) is marine oil, especially fish and algae. By modification of cow rations, EPA and …


New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin Jan 2014

New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin

Turkish Journal of Veterinary & Animal Sciences

In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …


Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇ Jan 2014

Molecular, Cellular, And Physiological Determinants Of Bull Fertility, Alexis Marie Parisi, Shannon Kate Thompson, Abdullah Kaya, Erdoğan Memi̇li̇

Turkish Journal of Veterinary & Animal Sciences

Bull fertility, defined as the ability of the spermatozoon to fertilize and activate the ovum and then support embryonic and even fetal development, is a crucial factor influencing animal reproduction and production efficiency. Despite significant influences of fertility on herd genetic improvement and efficient cattle production, the mechanisms regulating this phenotype and reliable biomarkers associated with bull fertility are poorly defined. Specifically, there is a broad lack of knowledge in bull fertility. In this paper, bull fertility is reviewed within the context of animal systems physiology, and system biology markers associated with bull fertility are provided. This mini-review is intended …


Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky Jan 2014

Antioxidant Supplementation And Purification Of Semen For Improved Artificial Insemination In Livestock Species, Peter Petruska, Marcela Capcarova, Peter Sutovsky

Turkish Journal of Veterinary & Animal Sciences

Improving sire reproductive performance in artificial insemination (AI) programs and maximizing the use of sires with valuable genomes are high on the list of priorities for livestock breeders in the United States and worldwide. While fixed-time AI and accurate estrous detection have already ameliorated the reproductive management of cows and sows, substantial gains remain to be made in the selection and utilization of male animals for field AI. In addition to better, more objective evaluation of breeding soundness and semen quality, numbers of spermatozoa per AI dose could be reduced and numbers of AI doses per collection could be increased …


Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy Jan 2014

Cholesterol Efflux From Sperm: Approaches And Applications, Zahid Naseer, Ejaz Ahmad, Meli̇h Aksoy

Turkish Journal of Veterinary & Animal Sciences

Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides …


The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson Jan 2014

The Avian Ovary And Follicle Development: Some Comparative And Practical Insights, Alan L. Johnson

Turkish Journal of Veterinary & Animal Sciences

This review focuses on recent research related to the avian ovary and, in particular, cellular mechanisms mediating embryonic growth of the ovary, postembryonic follicular development, and the process of follicular selection. Attention is drawn to a variety of fundamental, yet unresolved, aspects of ovarian function, several of which are uniquely related to avian reproductive strategies. Some notable differences in ovarian function between birds and the most closely related extant group, the crocodilians, are illustrated. Moreover, because the sauropsid (reptilian and avian) and synapsid (mammalian) lineages diverged shortly after the emergence of amniotes some 340 million years ago, consideration is given …


Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh Jan 2014

Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh

Turkish Journal of Veterinary & Animal Sciences

In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (


Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy Jan 2014

Endocrine And Metabolic Mechanisms Of Embryo And Fetal Intrauterine Growth Retardation In Dairy Cows, Anatoliy Nezhdanov, Sergey Shabunin, Vitaliy Mikhalev, Nikolay Klimov, Anton Chernitskiy

Turkish Journal of Veterinary & Animal Sciences

This research was conducted to study hormonal and metabolic statuses and to determine ultrasound criteria for early diagnosis of fetal growth retardation syndrome (FGRS) in dairy cows of Black-motley (n = 41) and Red-motley (n = 56) Holstein breeds. Concentrations of progesterone, testosterone, 17beta-estradiol, dehydroepiandrosterone sulfate, cortisol, and triiodothyronine in blood serum were assessed by immune-enzyme analysis. Concentrations of plasma L-ascorbic acid and nitrogen oxides, serum immune globulin, middle molecular peptides, and bactericidal and lysozyme activity were determined by spectrophotometry. On days 38-40 and 60-65 of gestation, FGRS in cows was associated with hypoprogesteronemia, decrease of adrenal and thyroid gland …


Effect Of Inbreeding On Yield And Quality Of Embryos Recovered From Superovulated Holstein Cows, Jiri Bezdicek, Ludek Stadnik, Alexander Makarevich, Elena Kubovicova, Frantisek Louda, Zdenka Hegedusova, Radek Holasek, Jan Beran, Michaela Nejdlova Jan 2014

Effect Of Inbreeding On Yield And Quality Of Embryos Recovered From Superovulated Holstein Cows, Jiri Bezdicek, Ludek Stadnik, Alexander Makarevich, Elena Kubovicova, Frantisek Louda, Zdenka Hegedusova, Radek Holasek, Jan Beran, Michaela Nejdlova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effect of inbreeding on the yield and quality of bovine embryos. The data were obtained from 125 Holstein cows superovulated on 3 farms in the Czech Republic. In total, 125 flushings of embryos were analyzed, from which 761 embryos were obtained. Of all donors, 36 cows were inbred (199 embryos) and 89 cows were noninbred (562 embryos). The inbred cows were divided into 2 groups according to their inbreeding coefficient (FX) level. The low FX group (FX 1.26%-1.56%) had 23 cows and 147 embryos, and the high FX group (FX 3.1%-25%) …


The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova Jan 2014

The Effect Of Short-Term Lupin (Lupinus Angustifolius) Feed Supplementation On Serum Steroid Hormones, Insulin-Like Growth Factor I, And Ovarian Follicular Development And Atresia In Merino Ewes, Radoslava Vlckova, Jan Posivak, Igor Valocky, Zuzana Kravcova, Andrea Eibenová, Drahomia Sopkova

Turkish Journal of Veterinary & Animal Sciences

The effects of short-term lupin supplementation on ovulation rate in ewes during oestrous season are well known. In this study, we tested the effects of an 8-day lupin supplementation on folliculogenesis during anoestrus applied over the hormonal treatment of ewes. Fourteen anoestrous Merino ewes were assigned into 2 groups (n = 7 for each) in which the control group was fed a basal diet and the experimental group was supplemented with lupin groats (500 g per head per day). Both groups were treated intramuscularly with 12.5 µg/head lecirelinum (luteinising hormone releasing hormone super analogue) on the first day of supplementation …


Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh Jan 2014

Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh

Turkish Journal of Veterinary & Animal Sciences

Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 …


Expression Of Vascular Endothelial Growth Factor And Hypoxia-Inducible Factor-1 Alpha During The Periovulatory Period In Goats, Chainarong Navanukraw, Jiratti Thammasiri, Tossapol Moonmanee, Jaruk Nutthakornkul Jan 2014

Expression Of Vascular Endothelial Growth Factor And Hypoxia-Inducible Factor-1 Alpha During The Periovulatory Period In Goats, Chainarong Navanukraw, Jiratti Thammasiri, Tossapol Moonmanee, Jaruk Nutthakornkul

Turkish Journal of Veterinary & Animal Sciences

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.