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Understanding The Basis For Increased 2,4-D Tolerance In Red Clover (Trifolium Pratense): Field Evaluations, Metabolism, And Gene Expression, Lucas Pinheiro De Araujo

Theses and Dissertations--Plant and Soil Sciences

Incorporation of red clover (Trifolium pratense L.) into grass pastures offers several benefits. However, red clover cultivars available for Kentucky producers are highly susceptible to synthetic auxin herbicides, such as 2,4-D (2,4-dichlorophenoxy acetic acid), used for pasture broadleaf weed control. To overcome this problem, UK2014 red clover was bred for increased tolerance to 2,4-D. We compared the field performance of UK2014 to Kenland, a standard cultivar grown in the transition zone of the United States. UK2014 and Kenland were seeded in the springs of 2017 and 2018. Single applications of 0, 1.12, or 2.24 kg ae ha^-1 2,4-D …

Effects Of Cyclic Heat Stress On The Acute Inflammatory Response In Broilers, Alessandro J. Rocchi, Chrysta N. Beck, Jossie M. Santamaria, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Heat stress (HS) is a growing concern in broiler production. Little is known regarding the effect of HS on immune function. To examine the effects of HS on innate immunity, the local- and systemic-inflammatory responses to lipopolysaccharide (LPS) were examined in Cobb 500 male broiler chicks reared under thermoneutral (TN) or cyclic HS conditions. Beginning at four days of age, HS birds were subjected to 35 °C from 8:00 a.m. to 10:00 p.m. and TN temperatures from 10:00 p.m. to 8:00 a.m. At 37 days of age, four groups of broilers were formed: LPS-TN (8 broilers), phosphate-buffered saline (PBS)-TN (4 …

Dpird Grains Research And Development (R&D) Stakeholder Update 2023, Department Of Primary Industries And Regional Development, Western Australia

Grain and other field crops published reports

The Grains R&D (Research and Development) Stakeholder Update was developed to showcase the industry-leading work delivered by the Grains Research and Industry Development team at the Department of Primary Industries and Regional Development (DPIRD) in Western Australia (WA).

Grains R&D is critical to the ongoing productivity and international competitiveness of our industry and the economic prosperity of WA. This newsletter highlights some of the vital research helping farmers grow the industry.

Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden

Electronic Theses and Dissertations

Regenerative agriculture is a pressing matter for the dairy industry to address cropland sustainability and carbon sequestration. One regenerative management practice that has been identified to help with row crop sustainability for key metrics like soil organic carbon (SOC) is complex covers. When producers use complex covers one of the main challenges is that it takes time to detect a change in SOC. However, simulation models are a tool that can be used to help determine if a regenerative practice is a strategy that gives the best results (i.e., increased SOC) while aligning with long-term production goals. Therefore, our objectives …

Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen

Electronic Theses and Dissertations

High-performance liquid chromatography (HPLC) is a form of analytical chemistry where adsorption is a key factor in which the purpose is to separate, identify and quantify each component in a mixture. It is the optimal separation technique of chemical and biological compounds that are non-volatile including proteins and natural products (i.e., plant extracts). As each sample component interacts differently with the absorbent material within the column, the flow rates change leading to the separation of peaks, which are plotted via computer software. Most commonly HPLC analysis has been used for chemical manufacturing and pharmaceutical purposes. When using HPLC for crop …

Genomics-Assisted Approaches To Improve Grain Yield And End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.), Harsimardeep Singh Gill

Electronic Theses and Dissertations

Global wheat production needs to be increased by 60% to meet the future demand of feeding nine billion people by 2050. Simultaneously, it is important to improve the enduse quality to meet the requirements of producers, grain markets, processors, and consumers. Thus, the development of more productive wheat varieties with better enduse quality remains the primary focus for all wheat breeding programs. However, direct phenotypic selection for improving grain yield and end-use quality is difficult as it is highly influenced by environmental factors. This dissertation focuses on harnessing advancements in genomics applications, including genome-wide association studies (GWAS), for the genetic …

Changes And Relationships Of Soil Aluminum, Organic Matter, And Hydrogen Ion Concentration With Rye Cover Crop, Arron Wilder

Cal Poly Humboldt theses and projects

Soil acidity is a common agricultural problem worldwide, as approximately 50% of all potentially arable soils are affected by pH limitations. At soil pH 3+) is considered to be the chemical form of aluminum in acid soil that hinders plant growth the most. Potentially, soil organic matter (SOM) can ameliorate the toxic effects of Al³⁺ on plants and microbes by binding with Al³⁺, thus preventing Al³⁺ (and other species of aluminum) from interacting in the rhizosphere. Increasing SOM also increases soil health indicators (i.e., microbial activity, soil water holding capacity, aggregate stability, porosity, etc.) while the …

Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima

Electronic Theses and Dissertations

Fusarium head blight (FHB), commonly called scab is the most devastating disease of wheat and barley caused by the ascomycete fungus, Fusarium graminearum. FHB affects both the quality and quantity of cereal grains affecting the supply chain of the food and feed industry and the marketability of the produce. Barley (Hordeum vulgare L.) is among the several crop species most vulnerable to FHB infection. An estimated 25% of Spring barley grain production in the United States is used to make malted beverages such as beer. Malting barley is a relatively high-value crop with high demands regarding grain quality. FHB directly …

The Effects Of Residue Amounts And Variety Selection On The Development Of Wheat Fungal Pathogens In South Dakota, Abrielle Tembreull

Electronic Theses and Dissertations

Roughly 50% of South Dakota cropland is under the practice of no-till, with large increases in acres of no-till in the last 20 or so years. No-till is beneficial for Great Plains states, such as South Dakota, as soil residues are withheld. Residues are shown to increase soil health through retention of moisture and increase of microorganisms, but residues can serve as a source of inoculum for fungal pathogens that can overwinter on them. As wheat is one of the most important food crops in the world, it is important to protect this vital crop from diseases. Diseases of wheat …

Soil Profile Properties And Greenhouse Gas Emissions As Influenced By Long-Term Cattle Manure And Inorganic Fertilizer Applications Under Corn-Soybean-Spring Wheat Rotation In Eastern South Dakota, Anuoluwa Ojonoka Sangotayo

Electronic Theses and Dissertations

The application of manure and inorganic fertilizer in row crops may significantly influence soil and greenhouse gas (GHG) emissions. Understanding the long-term influence of these management practices on soil pore characteristics, hydro-physical properties and greenhouse gas emission is essential in developing proper conservation practices. However, there is limited information on the impact of cattle manure and inorganic fertilizer application on soil hydro-physical properties, soil pore characteristics at lower depths and surface GHGs emissions. Therefore, the objectives of this study were to; (i) utilize X-ray computed tomography (XCT) technique to quantify the impact of manure and fertilizer amendments under a corn …

Forage Grass Growth Habit And Allelopathy In Birdsfoot Trefoil Germination And Establishment, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birdsfoot trefoil (Lotus corniculatus L.) (BFT) is a high quality, tanniniferous forage legume that has been shown to mitigate gastrointestinal nematode (GIN) infection in sheep. Reliable establishment of birdsfoot trefoil in existing pastures is difficult due to BFT’s poor seedling vigor, susceptibility to crown and root rot, and, perhaps chiefly, competition from other pasture plants. Most existing pastures in the Northeastern United States are primarily composed of cool-season grasses, many of which can be highly competitive. A common form of interplant competition is allelopathy, which occurs when secondary metabolites produced by a plant inhibit a necessary growth process of …

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan

Department of Agronomy and Horticulture: Faculty Publications

Background: Miscanthus, a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariforus and M. sinensis. Use of biotechnology-based procedures to genetically improve Miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Results: We developed gene editing procedures for Miscanthus using CRISPR/Cas9 that enabled the mutation of a specific (targeted) endogenous gene to knock out its function. Classified as paleo-allopolyploids (duplicated ancient …

New Semi-Dwarfing Alleles With Increased Coleoptile Length By Gene Editing Of Gibberellin 3-Oxidase 1 Using Crispr-Cas9 In Barley (Hordeum Vulgare L.), Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li

Grain and Other Field Crops Research Articles

The green revolution was based on genetic modification of the gibberellin (GA) hormone system with “dwarfing” gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA-related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3-oxidase1 (GA3ox1) as an alternative semi-dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large-scale field trials with an extensive collection of barley accessions, we showed that a natural …

Yield Prediction Through Integration Of Genetic, Environment, And Management Data Through Deep Learning, Daniel R. Kick, Jason G. Wallace, James C. Schnable, Judith M. Kolkman, Barış Alaca, Timothy M. Beissinger, Jode Edwards, David Ertl, Sherry Flint-Garcia, Joseph L. Gage, Candice N. Hirsch, Joseph E. Knoll, Natalia De Leon, Dayane C. Lima, Danilo E. Moreta, Maninder P. Singh, Addie Thompson, Teclemariam Weldekidan, Jacob D. Washburn

Department of Agronomy and Horticulture: Faculty Publications

Accurate prediction of the phenotypic outcomes produced by different combinations of genotypes, environments, and management interventions remains a key goal in biology with direct applications to agriculture, research, and conservation. The past decades have seen an expansion of new methods applied toward this goal. Here we predict maize yield using deep neural networks, compare the efficacy of 2 model development methods, and contextualize model performance using conventional linear and machine learning models. We examine the usefulness of incorporating interactions between disparate data types. We find deep learning and best linear unbiased predictor (BLUP) models with interactions had the best overall …

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Department of Agronomy and Horticulture: Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …

Is There An Economic Advantage To Planting Diverse Summer Annual Forage Mixtures?, Kelly Mercier, Chris Teutsch, Ray Smith, Kenny Burdine, Edwin Ritchey, Eric Vanzant

Journal of Extension

This study examined economic implications of planting summer annual mixtures of grasses, legumes, and forbs at varying nitrogen rates. No differences in yield occurred between the three mixtures, indicating that mixtures with lowest seed cost will be most economical. Applying N resulted in yield increases of 12.26 lb DM per lb N applied. Although yield responses to N were positive, sensitivity analyses showed that applying N resulted in positive net returns only when hay prices were high and N prices were low. When utilization rates are accounted for, enterprise budgets determined grazing to be 18% cheaper to implement than haying.

Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam

Department of Agronomy and Horticulture: Faculty Publications

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F₁ progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F₁ family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F₁ families were used in pairwise crosses to generate pseudo-F₂ families. Segregation (resistance or susceptibility) of progenies from the F₂ families in response to a …

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Department of Agronomy and Horticulture: Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA¹ . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets² , and consumers have not changed from traditional staples to whole-grain options³ . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake⁴ . We believe that …

Studi Pendahuluan Regenerasi Eksplan Teh Sebagai Upaya Percepatan Penyediaan Bibit Unggul Secara In Vitro, Intan Ratna Dewi Anjarsari, Erni Suminar, Murgayanti

Jurnal Kultivasi

Pembiakan generatif tanaman teh yang dilakukan dengan biji, sementara secara vegetatif dengan setek tunas yang mempunyai kelemahan, antara lain jumlah bibit yang dihasilkan terbatas, perlu waktu lama untuk menyeleksi pohon induk, dan bibit yang dihasilkan kurang optimal dalam penyerapan air dan unsur hara karena perakarannya dangkal sehingga kurang toleran terhadap kekeringan. Kultur jaringan menjadi salah satu alternatif dalam perbanyakan teh memperoleh klon teh unggul, seperti klon Seri Gambung 1-11, dalam jumlah banyak dan sifatnya yang seragam. Penelitian pendahuluan ini dilaksanakan di Laboratorium Kultur Jaringan Departemen Budidaya Pertanian Fakultas Pertanian UNPAD, mulai bulan Juni hingga Desember 2021. Penelitian terdiri dari dua …

Respons Pertumbuhan Biomassa Bagian Atas Bibit Kelapa Sawit Akibat Pemberian Kompos Pelepah Kelapa Sawit, Pupuk Hayati, Dan Asam Humat, Mira Ariyanti, Esnakelga Bernadetha Keliat, Cucu Suherman, Santi Rosniawaty, Mochamad Arief Soleh

Jurnal Kultivasi

Pemberian pupuk anorganik dapat meningkatkan pertumbuhan tanaman, tetapi penggunaan secara terus menerus menyebabkan dampak kurang baik bagi tanaman dan lingkungan pertanaman. Penelitian ini berkaitan dengan pentingnya penggunaan pupuk organik dalam mengurangi penggunaan pupuk anorganik sebagai salah satu langkah untuk mewujudkan perkebunan kelapa sawit (Elaeis guineensis Jacq.) yang berkelanjutan. Tujuan penelitian ini adalah untuk mengetahui respons pertumbuhan biomassa bagian atas bibit kelapa sawit akibat pemberian kompos pelepah kelapa sawit yang dikombinasikan dengan pupuk hayati dan asam humat. Percobaan dilaksanakan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran pada bulan Januari – Juli 2020. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) dengan …

Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh

Department of Agronomy and Horticulture: Faculty Publications

Narcissus tazetta var. chinensis (N. taz. var. chi.) accessions collected from the coastal areas of China, Korea, and Japan were compared with accessions of lower ranks of Narcissus tazetta (N. taz.) from Italy to study the genetic variations and with several morphologically similar cultivars available in the trade. Sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrITS) dendrogram and the chloroplast trnL-IS-trnF intergeneric spacer-gene (cpIS) were analyzed by maximum likelihood (ML) and posterior probability using STRUCTURE program. All single fower form of N. taz. var. chi. collected from China, Korea, and …

Potential Use Of Pgpr Based Biofertilizer For Improving The Nutrient Availability In Soil And Agronomic Efficiency Of Upland Rice, Rahma Tia Harahap, Diyan Herdiyantoro, Mieke Rochimi Setiawati, Isna Niar Rahmatul Azizah, Tualar Simarmata

Jurnal Kultivasi

Present study aimed to perform literature review to investigate the current status and potential use of plant growth promoting rhizobacteria (PGPR) for enhancing the soil plant’s health, plant growth and productivity in sustainable ways. The Systematic Literature Review (SLR) methods was applied according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) on published scientific literature from 2012-2022. Results revealed that genus diversity of PGPR (Bacillus sp., Serratia sp., Streptomyces sp., Pseudomonas sp., and Burkholderia sp.) produce siderophore and phytohormones such as indole-3-acetic acid (IAA), gibberellin (GAs), auxin, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, organic acids, biocontrol agent that …

Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa

Theses and Dissertations

The progressive increase in the global population and the rapidly changing climate have put unprecedented pressure on crop production. Cowpea is one of the world’s most important leguminous crops, contributing to food security and environmental sustainability. However, cowpea productivity is limited due to waterlogging stress. The main objective of this study was to explore physiological and biochemical mechanisms to understand how cowpea genotypes respond to waterlogging stress. Four studies were conducted in controlled and field conditions to achieve these objectives. Study 1 characterized the waterlogging tolerance of 30 cowpea genotypes in a controlled environment using 24 morphophysiological parameters with waterlogging …

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers

Theses and Dissertations

A long-held practice is to mix agricultural soil with a soil amendment to improve growing conditions in crops. A common soil amendment is biosolids produced from both municipal and dairy wastewater due to the macro- and micronutrients within it. Both the agricultural and wastewater industries are participating in the Circular Economy concept (CEC). Two experiments explored using expended bioelectrochemical systems (BES) that treated either synthetic dairy wastewater (DWW) or synthetic municipal wastewater (SWW) as soil amendments to improve corn plant growth when treated with three different nutrient treatments: 100%- 50%- and 0% Hoagland Nutrient Solutions. Biochar and used terracotta clay …

Effects Of Soil Management Practices On Water Infiltration And Maize Yield To Improve Mississippi River Valley Alluvial Aquifer Sustainability, Jacob Rix

Theses and Dissertations

Surface sealing and hardpans in loam soils are problematic in the Mid-South U.S. because of intense tillage. Two experiments quantified the effects of soil management practices on infiltration and maize yield in loam soils. The first study measured the impacts of in-row subsoiling frequency × furrow irrigation frequency. In-row subsoiling significantly improved the infiltration of irrigation but not the infiltration of precipitation. In-row subsoiling with low irrigation frequency is optimal to achieve profitable maize yields while encouraging groundwater conservation. In the second study, six soil management treatments were imposed. For single-ring infiltrometer tests, infiltration rates were different between the two …

Biological Remediation Of Fragipan, Corey Hale

Honors College Theses

The fragipan layer in the soil is a naturally occurring subsurface layer that restricts water percolation as well as inhibits root growth. A fragipan layer can be found almost ubiquitously across Southwestern Kentucky. This limits how agriculturalists manage soils in this area. It has been recently discovered by the University of Kentucky that Annual Ryegrass roots produce 3,4 dihydroxyphenylpropionic acid (DHPPA) that reacts with this hardpan to weaken the layer. Certain bacteria, such as Escherichia coli, Lactobacilli, and Bifidobacteria can also synthesize DHPPA through a breakdown process of chlorogenic acid. This reaction typically happens in the human digestive tract. …

Development Of Adaptive Rice Variety To Non-Tidal Swamp: Growth Evaluation Of Backcrossed Progenies (Bc1f1) And Its Parents, Inpago 5 And Inpara 8, Nabilah Amiros, Rujito Agus Suwignyo, Mery Hasmeda, Fikri Adriansyah, Entis Sutisna Halimi, Erizal Sodikin

Jurnal Kultivasi

Rice (Oryza sativa L.) cultivation in non-tidal swamp is severely constrained by abiotic stresses, i.e., submerged stress in the vegetative phase and drought stress in the generative phase. The development of rice varieties that have dual-tolerant to those abiotic stresses can improve adaptability and increase productivity. Efforts to obtain adaptive varieties under those abiotic stress conditions are being carried out by selecting the parents and crossing them, and has resulted in the population of BC1F1. The research was carried out on April – June 2021 at greenhouse of the Agriculture Faculty, Sriwijaya University. The study aimed to evaluate the growth …

A Review Of The Role Of Pollination On The Yield Of Cocoa Plant, Dani, Dewi Nur Rokhmah

Jurnal Kultivasi

Yield of cocoa (Theobroma cacao L.) plant, in form of cocoa seeds, is produced through the process of sexual reproduction. Pollination plays an important role in the successful formation of cocoa fruit and seeds. In addition, number of ovules per ovary, degree of self-incompatibility, and xenia effect are also affected the final yield. Those characters are determined by genetic factors. Cocoa flowers that are not pollinated fall within 24 hours and fail to develop into fruits and seeds. Pollination intensity up to a certain level showed a significant effect on increasing the percentage of fruit formation and the number of …

Application Of Legume Cover Crop And Various Vermicompost Dosages On Young Cinchona In The Marginal Area, Yudithia Maxiselly, Mira Ariyanti, Mochamad Arief Soleh, Naufal Fikri

Jurnal Kultivasi

Cinchona (Cinchona ledgeriana Moens) is one of the important medicinal plants that have the potential to cultivate as herbal medicine. This plant grows well in highland areas. Developing cinchona plants in the marginal area needs effort and modification techniques such as planting legume cover crop (LCC) to maintain the humidity and supply nutrition by fertilizing. Vermicompost is one of the organic fertilizers with rich nutrition to support plant growth. This research aimed to observe the effect of a combination of Mucuna bracteata as LCC and vermicompost dosage on young cinchona growth in a marginal area. This research was carried out …

Application Of Organic Fertilizer And Phosphate Solubilizing Bacteria To Increase The Growth And Yield Of Potatoes In Andisol, Agustina Erlinda Marpaung, Bina Karo, Susilawati Barus, Rina Christina Hutabarat, Rasiska Tarigan

Jurnal Kultivasi

Andisol has low phosphate (P) nutrient content due to high P fixation by allophane minerals, which affects crop production. The application of organic fertilizers and phosphate solubilizing bacteria can increase the availability of P in the soil in order to increase the production of potato plants. This study aimed to evaluate the effect of different types of organic fertilizer and phosphate solubilizing bacteria (PSB) on potato production. The research was conducted from August to November 2020 in the installation of research and assessment of agricultural technology Berastagi, Karo Regency, with an altitude of ± 1,340 m above sea level and …