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Articles 12301 - 12330 of 12682
Full-Text Articles in Entire DC Network
Managing Brome Grass In The Wheat:Lupin Rotation, Aik Hock Cheam, Gurget Gil, Christine Zaicou
Managing Brome Grass In The Wheat:Lupin Rotation, Aik Hock Cheam, Gurget Gil, Christine Zaicou
Journal of the Department of Agriculture, Western Australia, Series 4
Some farmers and scientists are questioning the sustainability of the cereal:lupin rotation in the Western Australian wheatbelt.
Being a 'tight' rotation, its continuation is constantly under threat by disease, especially lupin root rots caused by Pleiochaeta (the brown spot organism) and Rhizoctonia fungi. To control disease, some farmers have lengthened the rotation to three years, such as wheat:wheat:lupins or wheat:barley:lupins. The longer cereal phase also helps to stabilise soil against wind erosion. However, such rotations can lead to a rapid buildup of brome grass during the two consecutive years of cereals. The implications of these rotations on the severity of …
Opportunity Knocks : Sowing Wheat Early In The North-Eastern Wheatbelt, Nicole Kerr, Doug Abrecht
Opportunity Knocks : Sowing Wheat Early In The North-Eastern Wheatbelt, Nicole Kerr, Doug Abrecht
Journal of the Department of Agriculture, Western Australia, Series 4
Farmers consider many factors in deciding whether to start sowing wheat, but the most important are the amount of rain and time of year. In this study we assessed the chances of farmers in the north-eastern wheatbelt (less than 325 mm rainfall) receiving enough rain to sow wheat early. In low rainfall areas such as this, where an early finish to the season is likely, it is especially important that farmers take advantage of all early sowing opportunities. The potential benefits of sowing wheat early generally outweigh the associated risks, particularly in lower rainfall areas. However, early sowing depends on …
Test Detects Resistant Green Peach Aphids In Lupins, Francoise Berlandier, Ian Dadour
Test Detects Resistant Green Peach Aphids In Lupins, Francoise Berlandier, Ian Dadour
Journal of the Department of Agriculture, Western Australia, Series 4
Confirmation of insecticide resistance in green peach aphid in Western Australia's lupin crops means farmers will have to choose insecticides much more carefully to control this pest. Resistance within an aphid population varies, and when resistant aphids are no longer exposed to insecticides, they may revert back to being susceptible in as little as one generation. Furthermore, when these revertant populations are exposed to the same insecticides in the future, resistance quickly reoccurs. In this article, the authors discuss a test to determine the extent of insecticide resistance in green peach aphid and how to manage it.
On The Trail Of The Native Budworm, Kevin Walden
On The Trail Of The Native Budworm, Kevin Walden
Journal of the Department of Agriculture, Western Australia, Series 4
Native budworm, the worst pest of lupins, is a bit of a uiill-o-the-unsp. Some years the native budworm moths go virtually unnoticed. In others, they appear in plague proportions without warning - in these years, the cost to growers in lost yield runs into millions of dollars.
If an early warning system can be developed to predict the extent of moth flights into lupin crops, and this seems possible, major crop losses could be averted.
The Department of Agriculture has started research to develop a system of predicting the size of spring flights of native budworm moth. We need to …
Jasmonate, Genes, And Fragrant Signals, Paul E. Staswick
Jasmonate, Genes, And Fragrant Signals, Paul E. Staswick
Department of Agronomy and Horticulture: Faculty Publications
Although first recognized for its growth-inhibiting activity about 20 years ago, JA2 and its fragrant methyl ester MeJA (referred to here collectively as jasmonate) are receiving renewed interest as potentially important signaling molecules in plants. This is because jasmonate markedly increases the expression of specific plant genes, some of which are wound responsive. Similarities with fatty acid-derived stress signaling molecules in animals (1) make jasmonate of general biological interest.
A Review Of Plant Introductions To The Kimberley 1955 To 1987, R Mccartney
A Review Of Plant Introductions To The Kimberley 1955 To 1987, R Mccartney
Grain and other field crops published reports
This report summarizes the work done by the Department of Agriculture, Western Australia, in the west and north Kimberley in introducing plant species during 1955-1987.
For the purposes of this repor1the following areas are defined thus:
• West Kimberley- lies between latitude 17°5 and 19°5 and longitude 121°E and 127°E, with an extension to the north of 1° between 121°E and I 24°20'E. The area is 118,000 km".
• North Kimberley - lies north of latitude 17°5 and between longitude 124°20'E and 127°30'E. The area is 85,000 km'.
• East Kimberley-lies north of latitude 19°5 and between longitude 127°30'E and …
1991 Catalogue Of The Australian Lupin Collection Including Field Evaluation Data For Wild, Semi-Domesticated And Fully Domesticated Accessions, J. C. Clements, W. A. Cowling
1991 Catalogue Of The Australian Lupin Collection Including Field Evaluation Data For Wild, Semi-Domesticated And Fully Domesticated Accessions, J. C. Clements, W. A. Cowling
Grain and other field crops research reports
This report lists all wild, semi-domesticated and fully domesticated accessions in the Australian Lupin Collection to 1991. The Collection is composed of 2,264 accessions (Perth Numbers between 20629 and 27067) introduced to Australia or from naturalized populations and breeding programmes within Australia. Several accessions are selections from variable populations which were identified during field trials in South Perth, Western Australia. This catalogue lists the characteristics and field evaluation data for every accession where available. Plant breeders and crop scientists will find this information useful for the selection of lupin genotypes with desired characteristics.
Section A of this catalogue should be …
Review Of The Wild Oat Inflorescence And Seed: Anatomy, Development And Morphology, M. V. S. Raju, David M. Sutherland
Review Of The Wild Oat Inflorescence And Seed: Anatomy, Development And Morphology, M. V. S. Raju, David M. Sutherland
Biology Faculty Publications
This slim volume describes a detailed study of the reproductive parts of Avena fatua, the wild oat plant-a common weed in the northern plains and the probable ancestor of the cultivated oat. The book integrates the author's own work with information from available literature and includes lengthy technical descriptions of the structure and the growth of the inflorescence, the floret, the ovule, the pollen grain, the embryo, the seed, and the young seedling. Throughout the work, the author relates the wild oat's structure and development to other grasses, other monocotyledons, and other seed plants, offering evolutionary interpretations of many of …
Miscellaneous Publication 28/91 - Midas (Model Of An Integrated Dryland Agricultural System), David J. Pannell, Andrew Bathgate
Miscellaneous Publication 28/91 - Midas (Model Of An Integrated Dryland Agricultural System), David J. Pannell, Andrew Bathgate
Farm Systems and Economics published reports
MIDAS is a bioeconomic model of mixed crop/livestock farms in Western Australia. It selects farm management strategies which maximise profit subject to resource, technical and environmental constraints using the linear programming technique. This document provides a thorough documentation of the version of MIDAS developed for the "eastern wheatbelt" region. If you are learning to use MIDAS with the MARG menu driven system, you need to read the MARG manual as well as this documentation.
From early in the life of MIDAS, we gave a high priority to making the data and assumptions readily accessible in documentation and in microcomputer spreadsheet …
Propagation Of Yellow Bells (Geleznowia Verrucosa Turcz., Rutaceae) From Seed, Blakely Paynter, Kingsley Dixon
Propagation Of Yellow Bells (Geleznowia Verrucosa Turcz., Rutaceae) From Seed, Blakely Paynter, Kingsley Dixon
Grain and Other Field Crops Research Articles
Geleznowia verrucosa Turcz. (Rutaceae) is a commercially important, bush-picked wildflower that has no known commercial means of propagation. This study examined factors influencing seed dormancy and likely to restrict germination of the species. Germination of entire, untreated seed was low (less than 2%), but removal of a small portion of the testa at or near the radicle end of the seed improved germination. This response was affected by the temperature under which the seed germinated, but was unaffected by exposure of the seed to light while germinating. The percentage germination of nicked seeds 52 days after sowing increased from 8% …
Proceedings International Sorghum And Millet Crsp Conference, Timothy Schilling, Gary Odvody, Gebisa Ejeta, Larry Claflin, Gary Peterson, Lloyd Rooney, Jerry Eastin, Joan Frederick
Proceedings International Sorghum And Millet Crsp Conference, Timothy Schilling, Gary Odvody, Gebisa Ejeta, Larry Claflin, Gary Peterson, Lloyd Rooney, Jerry Eastin, Joan Frederick
INTSORMIL Impacts and Bulletins
On behalf of the INTSORMIL Board of Directors, Principal Investigators and the Management Entity Office, it gives me great pleasure this moming to welcome you to this opening session of the 1991 INTSORMIL International SorghumIMlllet CRSP Conference.
INTSORMIL initiated the Biennial CRSP conference series in 1983. Attendance has grown with each meeting. Today we have 199 persons registered from 12 States in the U.S. and 27 different countries. There are representatives from three International Agricultural Research Centers (lCRISAT, IFPRI, and ICRISAT), four private seed companies and the U.S. National Grain Sorghum Producers Association (NGSPA).
I look around this audience and …
Bulletin No 4220 - Growing High Yielding Wheat Crops In The Central Wheatbelt, Michael Perry
Bulletin No 4220 - Growing High Yielding Wheat Crops In The Central Wheatbelt, Michael Perry
Bulletins 4000 -
In this Bulletin we hope to provide you with what we believe is the best and most up-to-date information about growing wheat in your particular environment.
It is now more important than ever for producers to get the most out of their resources of land, labour and capital. The key to doing that is the knowledge to do even better what you probably do well already-growing wheat.
How Potassium Affects Emergence Of Five Supersweet Corn (Zea Mays L. Var. Rugosa) Cultivars With Low Temperatures, Nombasa Tsengwa
How Potassium Affects Emergence Of Five Supersweet Corn (Zea Mays L. Var. Rugosa) Cultivars With Low Temperatures, Nombasa Tsengwa
Masters Theses
Supersweet types of sweet corn (Zea mays L. var rugosa) are sensitive to low temperature during emergence and thus have difficulty in establishing stands in early spring. This study investigated how banded potash affected establishment of five supersweet cultivars of sweet corn in fields and growth chambers.
In the field, sweet corn cultivars were planted near Urbana, Il on April 18 and July 22, 1991 with three levels of potash (0, 45 and 90 kg/ha) banded at planting. Several parameters of growth and development were measured approximately 2, 4 and 6 weeks after planting. No significant effect of potash was …
Inheritance Of Acid-Soil Tolerance In Sorghum (Sorghum Bicolor) Grown On An Ultisol, C. I. Flores, L. M. Gourley, Jeffrey F. Pedersen, R. B. Clark
Inheritance Of Acid-Soil Tolerance In Sorghum (Sorghum Bicolor) Grown On An Ultisol, C. I. Flores, L. M. Gourley, Jeffrey F. Pedersen, R. B. Clark
Department of Agronomy and Horticulture: Faculty Publications
Inheritance of acid-soil tolerance (generally considered AI-toxicity tolerance) i.n sorghum [Sorghum bicolor (L.) Moench] is not clear. Forty F1 sorghum hybrids and their 14 parents were grown two seasons in the field at relatively high (67 and 71%) and low (43 and 42%) Al saturations on an acid Ultisol in Colombia, South America to evaluate the effects of acid soil on agronomic component traits and to better understand inheritance of acid-soil tolerance of sorghum. For plants grown at the high Al saturation levels, hybrids from acid-soil tolerant [AS-T] x acid soil-sensitive [AS-S] crosses were as tolerant as hybrids from …
Fertility And Forage Yield Of Sorghum X Sudangrass Hybrids In A1 And A3 Cytoplasm, J. J. Toy, Jeffrey F. Pedersen, K. J. Moore
Fertility And Forage Yield Of Sorghum X Sudangrass Hybrids In A1 And A3 Cytoplasm, J. J. Toy, Jeffrey F. Pedersen, K. J. Moore
Department of Agronomy and Horticulture: Faculty Publications
Most sorghum x sudangrass hybrids are currently produced in Al male-sterile cytoplasm. Availability of alternative cytoplasmic sterility systems allows production of sorghum x sudangrass hybrids that may have superior forage characteristics. A study was conducted to compare the agronomic performance of Al and A3 sorghum x sudangrass hybrids. A bulk of eight sudangrass populations was used to pollinate four grain sorghum inbreds normally used as females that had been sterilized in both Al and A3 cytoplasm, and two inbreds normally used as males that had been sterilized in A3 cytoplasm. The hybrids were evaluated in 1990. Results indicate that male-sterile …
Notes: Interpretive Analysis For Forage Yield Trial Data, Jeffrey F. Pedersen, K. J. Moore, Edzard Van Santen
Notes: Interpretive Analysis For Forage Yield Trial Data, Jeffrey F. Pedersen, K. J. Moore, Edzard Van Santen
Department of Agronomy and Horticulture: Faculty Publications
Forage cultivar evaluation is often done in small plots with multiple harvests throughout the growing season. Data is often summarized by presenting a yearly total yield for each cultivar in addition to the mean for each harvest date. Data summarization often becomes burdensome and difficult to interpret. Regressing yield against a growth index associated with harvest dates can be utilized to describe forage performance in a concise and easily interpreted format. Subsets of data from tall fescue (Festuca amndinacea Schreb.) yield trials conducted in Alabama and Kentucky were used to demonstrate the technique. The analysis involves regressing yield of a …
Nitrogen And Methyl Jasmonate Induction Of Soybean Vegetative Storage Protein Genes, Paul E. Staswick, Jing-Feng Huang, Yoon Rhee
Nitrogen And Methyl Jasmonate Induction Of Soybean Vegetative Storage Protein Genes, Paul E. Staswick, Jing-Feng Huang, Yoon Rhee
Department of Agronomy and Horticulture: Faculty Publications
Vegetative storage protein (VSP) and VSP mRNA levels in soybean (Glycine max) leaves correlated with the amount of NH4NO3 provided to nonnodulated plants. The mRNA level declined as leaves matured, but high levels of N delayed the decline. This is consistent with the proposed role for VSP in the temporary storage of N. Wounding, petiole girdling, and treatment with methyijasmonate (MeJA) increased VSP mRNA in leaves 24 hours after treatment. The magnitude of the response depended on leaf age and N availability. N deficiency essentially eliminated the response to wounding and petiole girdling. MeJA was …
Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby
Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby
Harold W. Manter Laboratory of Parasitology: Faculty Publications
Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …
Origin And Evolution Of Defective Interfering Rnas Of Tomato Bushy Stunt Virus, David A. Knorr, Thomas Jack Morris
Origin And Evolution Of Defective Interfering Rnas Of Tomato Bushy Stunt Virus, David A. Knorr, Thomas Jack Morris
School of Biological Sciences: Faculty Publications
Viruses with defective genomes have been identified in association with virtually every major family of viruses and have been widely utilized as tools for investigating virus functions in animal cell culture systems (Perrault, 1981). It is generally thought that defective interfering viruses (DIs) arise through deletion, rearrangement, or recombination of a competent viral genome. DIs tack the ability for independent existence relying on their parental helper viruses to supply factors required for replication, maturation, and/or encapsidation (Huang and Baltimore, 1977). The interference attributed to DIs is thought to result from competition with the helper virus for factors required in trans …
Sorghum Nutritional Quality, Gebisa Ejeta, Edwin T. Mertz, Lloyd Rooney, Robert Schaffer, John M. Yohe
Sorghum Nutritional Quality, Gebisa Ejeta, Edwin T. Mertz, Lloyd Rooney, Robert Schaffer, John M. Yohe
INTSORMIL Impacts and Bulletins
Table of Contents
Opening Session Page
Introduction, D. W. Thomas
Purpose of Conference, R. Schaffert
The Sorghum Producers Views on Sorghum Quality Improvement, L. Rooney
Importance of Sorghum on a Global Scale, B. Maunder
Relevance of International Sorghum and Millet Research to U.S. Agriculture, J. M. Yohe
Pleiochaeta Diseases Of Lupins, Mark Sweetingham
Pleiochaeta Diseases Of Lupins, Mark Sweetingham
Experimental Summaries - Plant Research
Trial 90WH20, 90SC5, 90LG8
Rates of dicarboximide fungicides for lupins.
Location: Wongan Hills Research Station, South Carrabin Research Station, East Hyden.
To compare the disease control and yield response of Rovral and Sumisclex seed treatments at a range of rates.
Trial 90M11, 90BA17
Rates of dicarboxide fungicides for lupins.
Location: Merredin Research Station, Badgingarra Research Station.
To compare the disease control and yield response of Rovral and Sumisclex seed treatments at a range of rates,
Role Of Assimilate Partition And Biomass Productivity In Wheat Yield Improvement., K. H.M. Siddique
Role Of Assimilate Partition And Biomass Productivity In Wheat Yield Improvement., K. H.M. Siddique
Experimental Summaries - Plant Research
Trial 90WH75
A. Ear:stem ratios in parental genotypes.
Earlier studies have established the importance of ear:stem ratio in determining the harvest index and potential yield of wheat cultivars (Siddique et al. 1989; Siddique and Whan, 1990). Much of the earlier work was concentrated mainly on recommended high yielding cultivars and some old cultivars. It is important to evaluate a wide range of parental materials for ear:stem ratios, before embarking on a breeding programme on ear:stem ratios. The main aim of this study was to assess ear:stem ratios in a wide range of parental genotypes.
B. Evaluation of breeding populations for …
Waterlogging : How It Reduces Plant Growth And How Plants Can Overcome Its Effects, Tim Setter, Bob Belford
Waterlogging : How It Reduces Plant Growth And How Plants Can Overcome Its Effects, Tim Setter, Bob Belford
Journal of the Department of Agriculture, Western Australia, Series 4
Lack of oxygen is the major cause of limited plant growth in waterlogged soils. When soils become waterlogged less gas diffuses to and from the roots through the soil pores; there are changes in concentrations of mineral elements in soil solutions; and toxic products of roots and soil microorganisms begin to accumulate. These changes adversely affect germination, growth and development of plants.
A Survey Using 14n:15n Natural Abundance Of The N Fixation By Pasture Legumes In The Field And Of Factors Affecting Fixation, P. Sanford
Experimental Summaries - Plant Research
Trial 89AL43, 90AL50, 90KO16
A preliminary survey using 14N:15N natural abundance to assess N2 fixation by pasture legumes in the field.
Location: Albany.
1. Develop a sampling methodology for the 1990 farm survey.
2. An initial assessment of nitrogen fixation by pasture legumes in the field.
Trial 90MT70
Using 14N:15N natural abundance to assess N2-fixation by subterranean clover.
Location Mount Barker Research Station
To examine symbiotic nitrogen fixation by subterranean clover, in detail, through one season in a productive pasture. To better assess the point in the season at which as one time sampling would most accurately determine the proportion …
Understanding Crop Growth On Duplex Soils., R. Belford, Miles Dracup, D. Tennant
Understanding Crop Growth On Duplex Soils., R. Belford, Miles Dracup, D. Tennant
Experimental Summaries - Plant Research
Trial 88EB18
Location: East Beverley Research Station
Impeded early root growth.
Using aerial photography and crop growth data from previous seasons we identified areas on the site where crops grow well or poorly. One of the factors implicated in the poor growth areas is impeded early root growth (see Belford et al. and Tennant et at., 1990 Experimental Summaries, this section). Here we describe the problem and outline possible causes.
Trial 88EB18
Growth and yield of wheat and lupin crops at East Beverley.
Location: East Beverley Research Station.
To measure the growth and yield of wheat and lupin crops in …
Evaluation Of Seeding Rates Of Au Triumph And Endophyte-Infected Kentucky 31 Tall Fescue, Jeffrey F. Pedersen, D. M. Ball
Evaluation Of Seeding Rates Of Au Triumph And Endophyte-Infected Kentucky 31 Tall Fescue, Jeffrey F. Pedersen, D. M. Ball
Department of Agronomy and Horticulture: Faculty Publications
Increased difficulty in stand establishment of tall fescue (Festuca arundinacea Schreb.) has been reported by producers since the introduction of endophyte (Acremonium coenophialum Morgan Jones and Gams)-free tall fescue seed into the market place. This has encouraged the use of higher seeding rates for endophyte-free tall fescue without scientific basis. The objective of this study was to determine whether ‘AU Triumph’, and endophyte-free cultivar, and endophyte-infected ‘Kentucky 31’ differ in minimum seeding rates for successful establishment. Experiments were established at two locations in each of two years, using a randomized complete block with four replications. Soils at the two locations …
Heritability Of Root Characteristics Affecting Mineral Uptake In Tall Fescue, J. H. Edwards, Jeffrey F. Pedersen, R. C. Kingery
Heritability Of Root Characteristics Affecting Mineral Uptake In Tall Fescue, J. H. Edwards, Jeffrey F. Pedersen, R. C. Kingery
Department of Agronomy and Horticulture: Faculty Publications
Modification of plant roots can potentially increase the area. of adaptation of tall fescue (Festuca arundinacea Schreb.). Limited information is available on the heritability of root diameter and root volume in tall fescue. A greenhouse experiment was conducted with'50 random 'Kentucky 31' (Ky-31) tall fescue parents and their progeny grown in aerated nutrient solution during four separate time intervals. Measurements of root volume, root diameter, shoot Mg concentration, and shoot K/(Mg + Ca) ratio were made six weeks after clonal material was transplanted in nutrient solution. Heritability estimates for root volume, root diameter, shoot Mg concentration, and shoot K/(Mg …
A Review Of The Agronomic Characteristics Of Endophyte-Free And Endophyte-Infected Tall Fescue, Jeffrey F. Pedersen, G. D. Lacefield, D. M. Ball
A Review Of The Agronomic Characteristics Of Endophyte-Free And Endophyte-Infected Tall Fescue, Jeffrey F. Pedersen, G. D. Lacefield, D. M. Ball
Department of Agronomy and Horticulture: Faculty Publications
Agronomic differences between endophyte- (Acremonium coenophialum Morgan Jones and Gams) free and endophyte-infected tall fescue (Festuca arundinacea Schreb.) exist, and should be considered when implementing a management strategy. Although dry matter yield of tall fescue does not generally appear to be influenced by endophyte infection status, endophyte infection has been shown to improve seedling performance and survival, is associated with insect and nematode resistance, drought resistance, improved nitrogen assimilation, and higher seed set. Considering all biologically valuable characters of the endophyte-tall fescue relationship, survival of endophyte-infected tall fescue is probably better than that of endophyte-free tall fescue, especially in drought- …
Fescues With Large Roots Are Drought Tolerant, H. A. Torbert, J. H. Edwards, Jeffrey F. Pedersen
Fescues With Large Roots Are Drought Tolerant, H. A. Torbert, J. H. Edwards, Jeffrey F. Pedersen
Department of Agronomy and Horticulture: Faculty Publications
Consideration of root/soil interactions is essential in, adapting tall fescue (Festuca arundinacea Schreb.) to soil and climatic conditions of the Coastal Plain region of the southeast. Sandy soils of the region are very susceptible to the formation of hardpans. These compacted layers often restrict plant root development to the plow layer (0 to 6 in.) and prevent plant roots from reaching available moisture and nutrients in the subsoil horizons. Plants grown under these conditions are shallow rooted and will be subjected to water stress up to 50% of the time during the growing season. Although implements are available to penetrate …
Can Genetically Engineered Crops Become Weeds?, Kathleen H. Keeler
Can Genetically Engineered Crops Become Weeds?, Kathleen H. Keeler
School of Biological Sciences: Faculty Publications
There are significant differences if the distribution of weedy characteristics among weeds, normal plants, and crops. The world’s most serious weeds possess on the average 10 or 11 of these characters, a random collection of British plants have an average seven of the traits, and crop plants only five. For the average crop to become as “weedy” as the average weed, it would need to acquire five weedy traits. Even using the unlikely assumption that those traits are single loci in which a dominant mutation would provide the weedy character, this would require the simultaneous acquisition of five gene substitutions. …