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Overview Of The Integrated Pest Management (Ipm) Terrain And Activities In Furtherance Of The Walmart Initiative To Purchase Fresh Vegetables From Local Growers In Alabama, Franklin Quarcoo, Conrad Bonsi

Professional Agricultural Workers Journal

Tuskegee University has been providing various types of technical expertise to limited resource farmers who have been supplying Walmart with collard greens, watermelons and purple hull peas. A number of pests bedevil the cultivation of these crops; cost-effective management methods for these pests are needed. The objectives of this paper are to document the IPM activities associated with supplying produce to Walmart; summarize pest problems encountered on the afore-stated crops; recommend IPM methods suitable for limited resource farmers; and suggest other activities that ensure that farmers incur even less pest-related crop losses. Anthracnose was the major pest encountered on watermelons. …

Nitrogen Rate Optimization For Grain Yield Within Fall And Spring Applications, Justin Wheeler

Theses and Dissertations

This thesis is a comprehensive analysis of research, investigating the impact of fall and spring applied nitrogen at various rates on the vulnerability of fall applied nitrogen, nitrogen uptake, and corn yields. In addition, optimal nitrogen rates and timing were evaluated across East Central Illinois.

Application And Timing Effects Of Qoi And Dmi Fungicides And A Foliar Fertilizer On Overall Plant Health And Grain Yield In Corn, Jason Phillip Geis

Open Access Theses

The use of fungicides on corn has recently increased as a result of higher grain market prices, changes in cropping practices, higher disease incidence and severity, and the availability and marketing claims of new fungicides. Some of the marketed potential "plant health(TM)" benefits include improved tolerance to drought and heat, improved N utilization, and increased stalk strength. Previous studies have displayed delayed canopy senescence, changes in water use efficiency, and reduced ozone damage in controlled environments. Foliar fertilization has also increased in popularity in recent years due to an increase in grain prices, manufacturer claims, and product availability.

Large-scale field …

Effects Of Wheat Grain Moisture: Quality, Germination, And Relationship To Accumulated Growing Degree Days, Kirsten Thomas

Open Access Theses

Bread wheat (Triticum aestivum L.) is a cereal crop of global importance. As global demand increases, it is essential to increase the quality and efficiency of crop production. Harvesting wheat early provides an opportunity for increased grain quality, and it may also allow the grower to double-crop soybean ( Glycine max L.) after wheat more effectively. Our objectives were to determine if harvesting grain early, at high moisture would, 1) increase milling and baking quality and 2) improve germination potential. As a result of these objectives, we will develop a model to predict dry-down of wheat. Five soft red …

Quantifying Subsurface Hydrology Effects On Chemical Transport In Agriculture Drainage Ditches Using A 20 Meter Flume, Colton Eugene Yoder

Open Access Theses

Agriculture drainage ditches serve as the veins of the Midwestern agricultural landscapes. The transport of chemical fertilizers and pesticides in these ditches affects both local and downstream ecosystems. Although much research has already been conducted on chemical transport in streams and drainage ditches, as well as through drainage tiles, there has not been sufficient research on the effects of subsurface hydrology on nutrient storage and interactions between the stream water and the hyporheic zone. In this study, a 20-meter flume was filled with ditch sediment from Marshall Ditch at Purdue University's Agronomy Center for Research and Education in West Lafayette, …

Pengaruh Jarak Tanam Berbeda Pada Berbagai Dosis Pupuk Organik Terhadap Pertumbuhan Dan Hasil Jagung Hibrida P-12 Di Jatinangor, Agus Wahyudin, Ruminta, D. C. Bachtiar

Jurnal Kultivasi

Penelitian ini bertujuan untuk memperoleh jarak tanam dan dosis pupuk organik kandang domba yang memberikan pengaruh terbaik terhadap pertumbuhan dan hasil tanaman jagung Hibrida P-12. Percobaan dilaksanakan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Jatinangor, Kabupaten Sumedang, dengan ketinggian tempat ±750 meter di atas permukaan laut, dari bulan Maret sampai Juli 2013. Metode percobaan yang digunakan adalah Rancangan Acak Kelompok yang terdiri dari 9 perlakuan dan diulang tiga kali. Perlakuannya adalah sebagai berikut: Jarak tanam 75 x 25 cm + 2t/ha pupuk kandang, jarak tanam 75 x 25 cm + 1t/ha pupuk kandang, jarak tanam 75 x 25 cm …

Agricultural Management Decisions Impact Isoprene Emission And Physiology Of Arundo Donax, An Emerging Bioenergy Crop, Jason Charles Maxfield

Dissertations and Theses

Arundo donax (Giant Reed) is quickly being developed as a rapidly-growing, robust, and highly productive bioenergy crop, with large scale cultivation of this species planned for the Columbia River basin of the Pacific Northwest (USA). Despite its potential as a next generation biomass crop, relatively few studies have examined the physiological performance of A. donax under agricultural conditions. Unlike traditional crops, A. donax is known to be a high-emitter of the volatile compound isoprene, which may significantly impact regional air quality, but it has not been widely cultivated in North America and little is known about how this species will …

Qtl Architecture Of Reproductive Fitness Characters In Brassica Rapa, Jennifer M. Dechaine, Marcus T. Brock, Cynthia Weinig

All Faculty Scholarship for the College of the Sciences

Background

Reproductive output is critical to both agronomists seeking to increase seed yield and to evolutionary biologists interested in understanding natural selection. We examine the genetic architecture of diverse reproductive fitness traits in recombinant inbred lines (RILs) developed from a crop (seed oil) × wild-like (rapid cycling) genotype of Brassica rapa in field and greenhouse environments.

Results

Several fitness traits showed strong correlations and QTL-colocalization across environments (days to bolting, fruit length and seed color). Total fruit number was uncorrelated across environments and most QTL affecting this trait were correspondingly environment-specific. Most fitness components were positively correlated, consistent with life-history …

Effects Of Covering System, Sealing Time, And Packing On Fermentation, Nutritional Quality, And Organic Matter Loss Of Corn Silage In A Drive-Over Pile, Katie Rose Nutcher

Dairy Science

This project involved an experiment conducted using a 550-ton capacity drive-over pile of whole-plant corn silage. The main effects compared were: packing the final forage surface with a loader or crawler, delay or immediate sealing, and covering with standard plastic or an oxygen barrier film.

Numerous studies have shown that the absence of oxygen in silage stored in a bunker silo or pile is crucial for proper fermentation and to ensure the highest quality silage at feed out. When oxygen is allowed to permeate through the covering material, it leads to visible spoilage. This trial showed that the oxygen barrier …

Does The Conversion Of Grasslands To Row Crop Production In Semi-Aridareas Threaten Global Food Supplies, David E. Clay, Sharon A. Clay, Kurtis D. Reitsma, Barry H. Dunn, Alexander J. Smart, Gregg G. Carlson, David Horvath, James J. Stone

Agronomy, Horticulture and Plant Science Faculty Publications

In the world's semi-arid regions, high crop demands have produced short term economic incentives to convert food production on native grasslands to dryland row crop food production, while genetic enhancements and equipment have reduced the risk of crop failure. The objectives of this paper were to discuss (1) the importance of considering the long-term sustainability of changing land use in semi-arid regions; (2) the impact of extreme climatic events on ecosystem functioning; and (3) factors contributing to higher crop yields in semi-arid regions. Semi-arid regions contain fragile areas where extreme climate events may be a tipping point that converts an …

First Report Of Curvularia Inaequalis And Bipolaris Spicifera Causing Leaf Blight Of Buffalograss In Nebraska, B. S. Amaradasa, K. Amundsen

Department of Agronomy and Horticulture: Faculty Publications

During the summer of 2011, foliar blight was observed on buffalograss (Buchloë dactyloides (Nutt.) Engelm.) lawns in Lincoln and Waverly, Nebraska. Disease symptoms were common when buffalograss was growing above 30°C and in drought conditions. Disease symptoms began as dark brown oblong leaf spots, followed by leaf tip dieback and eventual blighting of entire tillers. Leaf infections would progress into patches of thinning turf. Diseased leaf pieces were rinsed in distilled water and placed on 1.5% water agar. Two mitosporic fungal species having conidial morphology of Curvularia and Bipolaris were isolated. Colonies of Curvularia isolates grown on ¼× PDA …

Prediction Of Total Phenols, Condensed Tannins, And 3-Deoxyanthocyanidins In Sorghum Grain Using Near-Infrared (Nir) Spectroscopy, Linda Dykes, Leo Hoffmann Jr., Ostilio Portillo-Ridriguez, William L. Rooney, Lloyd W. Rooney

INTSORMIL Scientific Publications

The reported high phenolic levels in sorghum have led an interest from sorghum breeding programs in developing and identifying germplasms with high phenolic levels, which require screening a large number of samples to find those with the highest levels. Since wet chemistry screening methods are slow, expensive, and destructive, the use of NIR calibration curves could be an alternative. The objectives of this project were to determine the variation range in total phenols, condensed tannins, and 3- deoxyanthocyanidin levels in a diverse set of sorghum genotypes and to assess the predictive value of NIR curves to estimate these compounds in …

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul V. Switzer, Ryan M. Cumming

Faculty Research & Creative Activity

Hand removal is often recommended as a method for small-scale control of Japanese beetles (Popillia japonica Newman). In this study, we investigated the effectiveness of daily hand removal for controlling damage by Japanese beetles on grape plants. We also investigated whether the timing of the removal (at 0800, 1400, or 1900 hours, or at all 3 periods) inßuenced the effectiveness of the technique. We found that hand removal signiÞcantly lowered the number of beetles on, and consequently the damage to, grape plants relative to nonremoval controls. Of the single removal treatments, removal of beetles at 1900 hours was most effective, …

Effectiveness Of Hand Removal For Small-Scale Management Of Japanese Beetles (Coleoptera: Scarabaeidae), Paul Switzer, Ryan Cumming

Faculty Research & Creative Activity

Hand removal is often recommended as a method for small-scale control of Japanese beetles (Popillia japonica Newman). In this study, we investigated the effectiveness of daily hand removal for controlling damage by Japanese beetles on grape plants. We also investigated whether the timing of the removal (at 0800, 1400, or 1900 hours, or at all 3 periods) inßuenced the effectiveness of the technique. We found that hand removal signiÞcantly lowered the number of beetles on, and consequently the damage to, grape plants relative to nonremoval controls. Of the single removal treatments, removal of beetles at 1900 hours was most effective, …

Quantitative Trait Locus Analysis Of Unsaturated Fatty Acids In A Recombinant Inbred Population Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is an important oilseed crop which produces about 30 %of the world’s edible vegetable oil. The quality of soybean oil is determined by its fatty acid composition. Soybean oil high in oleic and low in linolenic fatty acids is desirable for human consumption and other uses. The objectives of this study were to identify quantitative trait loci (QTLs) for unsaturated fatty acids and to evaluate the genetic effects of single QTL and QTL combinations in soybean. A population of recombinant inbred lines derived from the cross of SD02-4-59 X A02-381100 was evaluated for fatty …

Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones, Christopher J. Smallwood, Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Interest in soybean [Glycine max (L.) Merr.] isoflavones has increased in recent years owing to numerous reported health benefits. Consequently, quantitative trait loci (QTL) detection for marker-assisted breeding for isoflavones is being examined for genetic gains. This study sought to detect QTL for soybean isoflavones in a population of 274 recombinant inbred lines derived from a cross between ‘Essex’ and ‘Williams 82’ that were subdivided and tested by maturity (early, mid, and late). The field tests were conducted in three environments in 2009 (Knoxville, TN; Harrisburg, IL; and Stuttgart, AR). The population was genotyped with 480 polymorphic single nucleotide …

Transcriptomic And Physiological Characterization Of The Fefe Mutant Of Melon (Cucumis Melo) Reveals New Aspects Of Iron-Copper Crosstalk, Brian M. Waters, Samuel A. Mcinturf, Keenan Amundsen

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) and copper (Cu) homeostasis are tightly linked across biology. In previous work, Fe deficiency interacted with Cu-regulated genes and stimulated Cu accumulation. The C940-fe (fefe) Fe-uptake mutant of melon (Cucumis melo) was characterized, and the fefe mutant was used to test whether Cu deficiency could stimulate Fe uptake. Wild-type and fefe mutant transcriptomes were determined by RNA-seq under Fe and Cu deficiency. FeFe-regulated genes included core Fe uptake, metal homeostasis, and transcription factor genes. Numerous genes were regulated by both Fe and Cu. The fefe mutant was rescued by high Fe or by …

Arabidopsis Lipins, Pdat1 Acyltransferase, And Sdp1 Triacylglycerol Lipase Synergistically Direct Fatty Acids Toward Β-Oxidation, Thereby Maintaining Membrane Lipid Homeostasis, Jilian Fan, Chengshi Yan, Rebecca Roston, John Shanklin, Changcheng Xu

Department of Agronomy and Horticulture: Faculty Publications

Triacylglycerol (TAG) metabolism is a key aspect of intracellular lipid homeostasis in yeast and mammals, but its role in vegetative tissues of plants remains poorly defined. We previously reported that PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1 (PDAT1) is crucial for diverting fatty acids (FAs) from membrane lipid synthesis to TAG and thereby protecting against FA-induced cell death in leaves. Here, we show that overexpression of PDAT1 enhances the turnover of FAs in leaf lipids. Using the trigalactosyldiacylglycerol1-1 (tgd1-1) mutant, which displays substantially enhanced PDAT1- mediated TAG synthesis, we demonstrate that disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases …

Boron Nutrition Of Burley And Dark Tobacco, Laura Ann Frakes Mitchell

Theses and Dissertations--Plant and Soil Sciences

The incidences of suspected Boron (B) deficiency have increased recently in Kentucky tobacco fields, potentially due to recent changes in management practices. The symptoms observed in the field include; hollow stalk, stunted growth, deformed or no bud formation, small slits on the lower leaf midrib and uncontrollable breaking of the midrib approximately two inches from the stalk. B is a micronutrient tobacco needs in minute amounts, however excessive additions of B could cause toxicity. The objectives of this work were to 1) establish critical points for B sufficiency, 2) describe and define B deficiency and toxicity symptoms and 3) develop …

A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and has a role in sustainable agriculture owing to its ability to fix atmospheric nitrogen. We assembled 473 Mb of the 587-Mb genome and genetically anchored 98% of this sequence in 11 chromosome-scale pseudomolecules. We compared the genome for the common bean against the soybean genome to find changes in soybean resulting from polyploidy. Using resequencing of 60 wild individuals and 100 landraces from the genetically differentiated Mesoamerican and Andean gene pools, we confirmed 2 independent domestications from genetic pools that diverged before human colonization. …

Reducing Water Extractable Phosphorus In Poultry Litter Using Chitosan Treatment, Zachary Simpson, Brina Smith, David A. Zaharoff, Brian E. Haggard

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Phosphorus (P) is an important factor in the eutrophication of freshwater, and watershed sources include effluent discharges and the landscape. Poultry litter applied to the landscape can be a potential source of P, which is dependent on rainfall, runoff and dissolution. Chitosan, the deacetylated form of the biopolymer chitin, has been shown to have an effect on reducing water extractable phosphorus (WEP) in poultry litter when applied as a powder. The intent of this study was to measure the effect that poultry litter treatment (PLT), acetic acid and incubation time have on chitosan’s ability to reduce WEP in poultry litter. …

Effectiveness Of A Farm Field Trip, Bonnie S. Sigmon

Theses and Dissertations--Community & Leadership Development

The annual Sigmon Farm Tour was started in 1992 as an agricultural education program where students could experience being on a farm with the goal of increasing the agricultural literacy levels of the participants. Every year the entire 4th grade student population of Rockcastle County spends the day touring the farm and participating in experiential mini lessons given by the cooperating farm service and health agencies. The program has continued for 20+ years without an evaluation as to whether it is achieving its objectives. This evaluation will also exhibit the programs strengths and weakness so it can continue to improve. …

Development Of Fad7-1 Single Mutant Arabidopsis Thaliana Plants That Are Resistant To Aphids, Kaleb L. Vaughn, Carlos A. Avila, Carmen S. Padilla-Marcia, Fiona L. Goggin

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Aphids are a group of sap-feeding insects that attack most of the world’s crops. The loss of function of fatty acid desaturase7 (FAD7) in Solanum lycopersicum (tomato plant) induces aphid resistance that is dependent upon the accumulation of plant defense hormones such as salicylic acid (SA). Tomato lacks most of the genetic resources found in the model plant Arabidopsis (Arabidopsis thaliana). There is an analogous fad7-1 line of Arabidopsis; however, the line has a background mutation, the glabra-1 (gl1), that causes the absence of trichomes (small hairs), which are essential to plant defense. In order to study aphid resistance, a …

Shattercane X Als-Tolerant Sorghum F1 Hybrid And Shattercane Interference In Als-Tolerant Sorghum, Rodrigo Werle, Jared J. Schmidt, John Laborde, Angela Tran, Cody F. Creech, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

ALS-tolerant grain sorghum cultivars are expected to be available for farmers within the next few years. Knowing that: (i) crosses between sorghum and shattercane are likely to occur resulting in crop-to-weed gene flow; (ii) ALS-susceptible shattercane X ALS-tolerant grain sorghum F1 hybrids (hybrids) were ultimately resistant to ALS-herbicides under field conditions; and (iii) hybrid fitness is equal to, or greater than, the wild parent, we conducted a greenhouse study to compare the competitive effect of shattercane and hybrid on sorghum, and whether or not herbicide application would influence the competitive ability of the hybrid …

Common Sunflower Seedling Emergence Across The U.S. Midwest, Sharon A. Clay, Adam Davis, Anita Dille, John Lindquist, Analiza H. M. Ramirez, Christy Sprague, Graig Reicks, Frank Forcella

Department of Agronomy and Horticulture: Faculty Publications

Predictions of weed emergence can be used by practitioners to schedule POST weed management operations. Common sunflower seed from Kansas was used at six Midwestern U.S. sites to examine the variability that 16 climates had on common sunflower emergence. Nonlinear mixed effects models, using a flexible sigmoidal Weibull function that included thermal time, hydrothermal time, and a modified hydrothermal time (with accumulation starting from January 1 of each year), were developed to describe the emergence data. An iterative method was used to select an optimal base temperature (T_b) and base and ceiling soil matric potentials (ψ_b and …

Genotyping By Sequencing For Genomic Prediction In A Soybean Breeding Population, Diego Jarquin, Kyle Kocak, Luis Posadas, Katie Hyma, Joseph Jedlicka, George L. Graef, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Background: Advances in genotyping technology, such as genotyping by sequencing (GBS), are making genomic prediction more attractive to reduce breeding cycle times and costs associated with phenotyping. Genomic prediction and selection has been studied in several crop species, but no reports exist in soybean. The objectives of this study were (i) evaluate prospects for genomic selection using GBS in a typical soybean breeding program and (ii) evaluate the effect of GBS marker selection and imputation on genomic prediction accuracy. To achieve these objectives, a set of soybean lines sampled from the University of Nebraska Soybean Breeding Program were genotyped using …

Identification And Validation Of Quantitative Trait Loci For Seed Yield, Oil And Protein Contents In Two Recombinant Inbred Line Populations Of Soybean, Xianzhi Wang, Guo‑Liang Jiang, Marci Green, Roy A. Scott, Qijian Song, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean seeds contain high levels of oil and protein, and are the important sources of vegetable oil and plant protein for human consumption and livestock feed. Increased seed yield, oil and protein contents are the main objectives of soybean breeding. The objectives of this study were to identify and validate quantitative trait loci (QTLs) associated with seed yield, oil and protein contents in two recombinant inbred line populations, and to evaluate the consistency of QTLs across different environments, studies and genetic backgrounds. Both the mapping population (SD02- 4-59 × A02-381100) and validation population (SD02- 911 × SD00-1501) were phenotyped for …

Porocercospora Seminalis Gen. Et Comb. Nov., The Causal Organism Of Buffalograss False Smut, Bimal S. Amaradasa, Hugo Madrid, Johannes Z. Groenewald, Pedro W. Crous, Keenan L. Amundsen

Department of Agronomy and Horticulture: Faculty Publications

False smut caused by Cercospora seminalis is an important disease of buffalograss (Buchloe dactyloides) affecting seed production. The pathogen prevents normal caryopsis development and causes considerable yield loss and reduced seed germination. The current taxonomic placement of the false-smut causal pathogen in the genus Cercospora is incorrect based on its morphological characteristics and DNA phylogeny. In the present study the phylogenetic position of C. seminalis is clarified based on DNA sequence analysis of three loci namely the internal transcribed spacer (ITS) region, partial nuclear ribosomal large subunit (LSU) and partial sequences of the RNA polymerase II second largest …

Identification Of A Sphingolipid Α-Glucuronosyltransferase That Is Essential For Pollen Function In Arabidopsis, Emilie A. Rennie, Berit Ebert, Godfrey P. Miles, Rebecca E. Cahoon, Katy M. Christiansen, Solomon Stonebloom, Hoda Khatab, David Twell, Christopher J. Petzold, Paul D. Adams, Paul Dupree, Joshua L. Heazlewood, Edgar B. Cahoon, Henrik Vibe Scheller

Department of Agronomy and Horticulture: Faculty Publications

Glycosyl inositol phosphorylceramide (GIPC) sphingolipids are a major class of lipids in fungi, protozoans, and plants. GIPCs are abundant in the plasma membrane in plants, comprising around a quarter of the total lipids in these membranes. Plant GIPCs contain unique glycan decorations that include a conserved glucuronic acid (GlcA) residue and various additional sugars; however, no proteins responsible for glycosylating GIPCs have been identified to date. Here, we show that the Arabidopsis thaliana protein INOSITOL PHOSPHORYLCERAMIDE GLUCURONOSYLTRANSFERASE1 (IPUT1) transfers GlcA from UDP-GlcA to GIPCs. To demonstrate IPUT1 activity, we introduced the IPUT1 gene together with genes for a UDP-glucose dehydrogenase …

Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having differential response to low-N stress.

Results: Illumina RNA-sequencing detected several common differentially expressed genes (DEGs) between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three …