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A Novel Transcription Factor In Arabidopsis Thaliana Abiotic Stress Response, Achira S. Weerathunga Arachchilage

LSU New Orleans Theses and Dissertations

Plants respond to environmental stress by altering their gene expression. Under stress conditions some genes are activated and some genes are repressed. Even though a lot of work has been done to understand mechanisms of gene activation under abiotic stress very little information is available on how stress responsive genes are kept repressed under normal growth conditions. Recent work has revealed that plants use transcriptional repression as common mechanism of gene repression. Transcriptional repression is achieved by recruitment co-repressor complexes to the target genes. Recent studies have revealed that the co-repressor LUH complexes with SLK1 and SLK2 to silence Arabidopsis …

Extension Agriculture And Natural Resources In The U.S. Midwest: A Review And Analysis Of Challenges And Future Opportunities, Mahdi M. Al-Kaisi, Roger Wesley Elmore, Gerald A. Miller, David Kwaw-Mensah

Department of Agronomy and Horticulture: Faculty Publications

This review addresses key changes in U.S. agricultural extension and future opportunities. Agricultural extension has been a part of the land-grant university (LGU) system for more than 100 years. The Morrill Act of 1862 established the LGU system by authorizing states access to federally controlled land and funding for public institutions offering educational opportunities focusing on agriculture and mechanical arts for farmers and the working class. Current surveys in Iowa reveal changing trends in extension: more than 90% of farmers identified private-sector crop advisers as their primary source for recommendations, whereas more than 80% of those advisors identified Iowa State …

Assessing Foliar Ascorbate Content In A Rice Diversity Panel And In Selected Mapping Population Lines With Varying Levels Of Seedling Cold Tolerance, Sonia Elizabeth Castillo Gonzalez

Student Theses and Dissertations

Rice is economically, culturally, and socially important to many people in the world. Arkansas is the main rice producer in the USA. Climate change continues to increase the incidence of abiotic stresses such as cold, heat, drought, water excess, and soil salinity. These stresses pose a particular challenge to rice cultivation. Rice has lower yields under cold stress, particularly when plants are exposed to cold at the seedling and reproductive stages. Early spring plantings and field irrigation with underground cold water expose rice seedlings to cold stress. Previous studies in Arabidopsis thaliana have shown that lines engineered to have elevated …

Soybean Yield And Nodulation Response To Crop History And Inoculation, Stephen Mason, Tomie Galusha, Zaher Kmail

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] inoculation was imposed on a long-term continuous grain sorghum [Sorghum bicolor (L.) Moench] and soybean cropping systems study with and without manure application at Mead, NE. The objective was to determine the influence of 28-yr history of continuous grain sorghum and soybean production, inoculation, and manure on soybean yield and nodulation. Average 2-yr soybean grain yield was 0.5 Mg ha^–1 greater on plots with continuous grain sorghum crop history rather than soybean history, even after this history was broken by 2-yr crop rotation with grain sorghum in the 2 yr previous to …

Soil Moisture Affects Growing-Season Wildfire Size In The Southern Great Plains, Erik S. Krueger, Tyson E. Ochsner, David M. Engle, J. D. Carlson, Dirac L. Twidwell, Samuel D. Fuhlendorf

Department of Agronomy and Horticulture: Faculty Publications

The increasing availability of soil moisture data presents an opportunity for its use in wildfire danger assessments, but research regarding the influence of soil moisture on wildfires is scarce. Our objective was to identify relationships between soil moisture and wildfire size for Oklahoma wildfires during the growing (May-October) and dormant seasons (November-April). We hypothesized that soil moisture influences wildfire size when vegetation is growing but is less important when most vegetation is dead or dormant. Soil moisture, as fraction of available water capacity (FAW), and commonly measured weather variables were determined for 38,419 wildfires from 2000–2012. Wildfires were grouped by …

Molecular Genetic Aspects Of Iron And Copper Cross-Talk In Arabidopsis Thaliana, Qingyuan Xiang

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Iron (Fe) and copper (Cu) are essential micronutrients for plants and humans who consume plants. Their functions are tightly linked. For example, both of Fe and Cu work as cofactors in superoxide dismutase proteins to prevent reactive oxygen species damage. When Fe is removed from nutrient solution, plants uptake more Cu, but how plants sense and respond to Fe status is not fully understood. This thesis includes two projects that focus on Fe and Cu interaction, homeostasis and cross-talk in Arabidopsis thaliana.

Two Arabidopsis ecotypes, Kas-1 and Tsu-1, have difference in timing of Cu accumulation in rosettes under Fe …

Gopher Intrusion In Cropping Systems, Kenneth Romero, Paul Cardinalli

Agricultural Education and Communication

The purpose of this project was to determine if no till vs. till on gopher populations was more beneficial. Which is more efficient at controlling gophers and explain the damage they can cause and the diseases they can potentially vector. What are the different options of pest managements that have to be used when dealing with a gopher infestation in a tilled field vs. a no-tilled field.

The Effects Of Meloidogyne Incognita And Heterodera Glycines On The Yield And Quality Of Edamame In Arkansas, Juliet Fultz

Graduate Theses and Dissertations

Edamame (Glycine max), also known as vegetable soybean, was introduced to the United States from Japan in 1890 and has been growing in popularity as a nutrient-rich, low-sugar snack in recent years. In 2012, the American Vegetable Soybean and Edamame, Inc. established the first domestic commercial processing plant in Mulberry, Arkansas and contracted local growers for production. Since the crop is harvested when seed are immature, management practices are different from those for traditional soybean. Plant-parasitic nematodes, particularly Meloidogyne incognita (southern root-knot) and Heterodera glycines (soybean cyst), are common in Arkansas and are pests of concern for edamame growers. Edamame …

Improving Establishment Of Seeded Buffalograss, Luqi Li

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Buffalograss [Buchloë dactyloides (Nutt.) Engelm.] is a warm-season grass native to the North American Great Plains. Buffalograss is drought resistant, heat resistant and cold tolerant, and thus is well-adapted to many uses in areas that require low maintenance or erosion control. Since buffalograss is slow-growing, one challenge of establishing seeded buffalograss is to maximize seedling maturation and establishment before winter. We evaluated dormant seeding buffalograss in late fall and winter, when germination is not expected until soils warm in spring. We successfully dormant seeded ‘Sundancer’, ‘Bowie’ or ‘Cody’ buffalograss at 146 kg ha^-1 from late November though …

Cotton Injury Due To Soil- Or Foliar-Applied Herbicides: An Assessment Based On The Influences Of Genetic, Agronomic, And Environmental Factors, Brandon William Schrage

Graduate Theses and Dissertations

Increasing populations of glyphosate-resistant weeds, such as Palmer amaranth, have prompted growers to pursue alternative means of weed control in cotton. In many cropping systems, this means the utilization of older chemistries and residual herbicides. The goal of this research was to evaluate and understand the agronomic and environmental factors that affect the inconsistent injury often associated with these herbicides as well as determine the impact of Palmer amaranth emergence date on seed production, biomass, and cotton yield. Experiments were conducted in three counties in Arkansas giving a distinct range of climate and soil texture. Injury, biomass, and number of …

Goss's Bacterial Wilt Development And Clavibacter Michiganensis Subsp. Nebraskensis Interactions With Spray Adjuvants, Sarah A. Schlund

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Goss’s bacterial wilt and leaf blight of corn (Zea mays L.), causal agent Clavibacter michiganensis subsp. nebraskensis, was first confirmed in Dawson County, NE in 1969. Disease incidence decreased in the 1980’s due to various management strategies and disease developed sporadically until the early 2000’s when it re-emerged and was economically important. A Midwest, multistate survey conducted in 2011 suggested farming practices that may have contributed to the pathogen’s re-emergence. The use of agricultural pesticides was associated with Goss’s wilt. Since spray adjuvants are often used with pesticides, and physical characteristics of these adjuvants may enable infection of …

Field Screening Of Diverse Soybean Cultivars For Flood Tolerance, Jane Achando Khatenje Mokua

Graduate Theses and Dissertations

Flooding can significantly reduce soybean growth, development, and yield. Therefore, screening and identification of flood-tolerant soybeans will enhance development of cultivars that are well suited for flood-prone areas. Sets of screening tests were conducted in Stuttgart, Arkansas using three separate but related experiments, with the purpose of establishing effective flood tolerance screening protocol that can identify tolerant and sensitive cultivars. Each experiment was completely randomized with three replications. The first experiment involved screening of 256 maturity group (IV and V) cultivars. Flooding was imposed at the fifth-node (V5) or first-bloom (R1) stage for ten days. Post-flood visual ratings and stand …

Disaggregating The Effect Of Drought And Heat Stress During Flowering On Spikelet Fertility In Rice, Lisa Straussberger

Graduate Theses and Dissertations

Due to rice’s wide geographic distribution, extending from 50°N to 35°S, rice is

forecasted to be the most vulnerable crop to warming global climates. Previous studies have

predicted lower rice yields and increasing rice yield variability due to higher frequencies of heat

stress events, and a higher variability in precipitation patterns due to global warming. As such,

understanding the effects of drought and heat stress intensity and frequency on rice yields is of

upmost importance to feeding the growing global population.

Given that drought and high-temperature stress often occur together, it is essential to

disaggregate the two individual stressors and …

Available Nitrogen And Denitrification In Soil Altered By Ground Cover And Nutrient Source In An Organic Apple Orchard, Jade Nicole Ford

Graduate Theses and Dissertations

A shift in public demand towards more organic and locally produced fruit and vegetables has been occurring across the United States in recent years. A common practice in organic fruit production is the application of organic ground covers to supply nutrients while enhancing other soil properties. A need for research exists in the southern region of the U.S. examining the effects of regionally applicable ground cover and nutrient management on nitrogen availability and the microbial community to provide information to organic farmers in the region. Two studies were conducted to determine how 12 treatment combinations of four ground covers (compost, …

Use Of Hppd-Inhibiting Herbicides For Control Of Common Weeds In Arkansas And Current Status Of Herbicide-Resistance Among Echinochloa Populations In Arkansas, Clay Starkey

Graduate Theses and Dissertations

Herbicide-resistant weeds in Arkansas cause problems for growers. Up-to-date information and new technologies can help plan mitigation strategies to slow resistant weeds. The objectives of this research were to provide a ‘snapshot’ of herbicide-resistant Echinochloa spp. in rice producing counties, determine how much resistance has spread across the state, and understand the effectiveness of 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides for control of glyphosate-resistant (GR) Palmer amaranth relative to commercial standards currently labeled in soybean. To assess the prevalence of Echinochloa spp. resistance, 82 samples were collected from 23 rice producing counties in 2010. The samples were tested for resistance to commonly …

Utilization Of Weed Ecology, Tank Mixtures, And Application Technology To Improve Efficacy Of Herbicide Applications In Soybean, Christopher John Meyer

Graduate Theses and Dissertations

New herbicide-resistant soybean traits will increase the number of herbicides that can be applied in soybean and change the technology currently used to make herbicide applications in soybean. Palmer amaranth (Amaranthus palmeri) remains one of the most difficult weeds to manage in soybean and thus is the primary focus of this research. Therefore, experiments were conducted to investigate Palmer amaranth temporal emergence, control of common Arkansas weeds with new soybean herbicide programs, and the effect of application technology on dicamba-containing tank mixtures. Palmer amaranth emergence exceeded 400 plants m-2 for tillage treatments in 2013 and 2014, and tillage events typically …

Defining The Potassium Nutritional Requirements And Distribution Among Plant Parts Of Representative Soybean Cultivars From Different Maturity Groups, Md. Rasel Parvej

Graduate Theses and Dissertations

The potassium (K) requirement of soybean [Glycine max (L.) Merr.] was investigated to determine whether cultivar sensitivity to K deficiency was affected by growth habit (determinate or indeterminate) and how cultivars from each growth habit accumulate and distribute K among plant structures. We also diagnosed K deficiency across reproductive growth stages (R2-6) using trifoliolate leaf- and petiole-K concentrations and at harvest (R8) using seed-K concentration. Soybean responded similarly to K deficiency in terms of yield, selected yield components, and seed-K concentration, regardless of growth habit. The yield loss from K deficiency was greatest on the middle to upper nodes of …

The Effects Of Foliar Nutrient Applications On Split, Yield, And Internal Fruit Quality Of 'Wonderful' Pomegranate (Punica Granatum L.), John Matthew Chater

Master's Theses

Fruit split is the most important physiological disorder in pomegranate production, causing devastating crop losses worldwide. Foliar nutrient applications have been used experimentally to mitigate pomegranate fruit split but none have been conducted using the industry standard cultivar, Wonderful, and little is known about the effects of foliar nutrient applications on pomegranate. Additionally, investigations into putative health benefits of pomegranate fruit have increased interest in its production but limited evidence exists regarding effects of agricultural practices such as foliar fertilizer applications on internal fruit quality. ‘Wonderful’ pomegranate trees at 2 commercial orchards were treated with foliar applications of ZnSO_{4 …}

Validation Of Soil-Test-Based Phosphorus And Potassium Fertilizer Recommendations For Rice And Soybean, Matthew Scott Fryer

Graduate Theses and Dissertations

The science of soil-testing for nutrient management and fertilizer recommendations is widely accepted among scientists and agronomists. Although this science is unsurpassed in predicting soil nutrient availability, soil-test interpretations are seldom validated. Major research objectives for irrigated soybean [Glycine max (L.) Merr.] and direct-seeded, delayed-flood rice (Oryza sativa L.) were to: i) validate the accuracy of Mehlich-3 soil-test P (STP) and K (STK) interpretations and ii) published critical tissue-P and -K interpretations in predicting the yield response to fertilizer at different significance levels (p≤0.05 to 0.25), iii) examine how seed nutrient concentrations are influenced by fertilization and crop response to …

Distribution Of Herbicide-Resistant Shattercane And Johnsongrass Populations In Sorghum Production Areas Of Nebraska And Northern Kansas, Rodrigo Werle, Amit J. Jhala, Melinda K. Yerka, J. Anita Dille, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Overreliance on acetolactate synthase (ALS)-inhibiting herbicides for weed control during the 1990s resulted in selection of ALS-resistant shattercane [Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet ex Davidse] biotypes in Nebraska. The objective of this study was to assess the baseline presence of ALS-resistance in 190 shattercane and 59 johnsongrass [Sorghum halepense (L.) Pers.] populations collected across northern Kansas, northwestern Missouri, and southern Nebraska in 2013. In 2014, a preliminary field experiment was conducted to evaluate the presence of herbicide resistance in the aforementioned populations. Treatments consisted of four herbicides (clethodim {2-[1-[[(E)-3-chloroprop-2-enoxy]amino] propylidene]-5-(2-ethylsulfanylpropyl)cyclohexane-1,3-dione}, glyphosate [N-(phosphonomethyl) …

Canola: A Modern Crop For A Modern Era, Kenneth J. Roché

Doctor of Plant Health Program: Dissertations and Student Research

The University of Nebraska-Lincoln Doctor of Plant Health program is a professional doctorate program with a comprehensive approach to plants and agriculture. The program emphasizes a broad interdisciplinary education across all plant-related disciplines, practical learning, research, and experience through internships. For my final required internship, I worked as a senior agricultural research intern with Research Designed for Agriculture (RD4AG) in Montana. RD4AG is a contract research organization based in Yuma, AZ with over thirty-years of experience. During my three month internship at RD4AG in Montana, a large portion of my responsibilities centered on managing regulated canola trials that were undertaken …

Soybean Irrigation Initiation Timing Using Evapotranspiration And Soil Moisture Sensor Cues, Justin Chlapecka

Student Theses and Dissertations

Expanded use of irrigation management tools is needed to improve water use efficiency in eastern Arkansas soybean (Glycine max) production. Irrigation initiation timing was studied on a furrow irrigated, sandy loam commercial field in Mississippi County. A major objective was to develop, validate, and expand use of irrigation timing cues, incorporating local weather station data, atmometers, and soil moisture measurements. Four timing treatments were evaluated: early, recommended, late, and rainfed, and the crop and pest response to each. With above average rainfall in 2014 and 2015, results showed little variation of soybean yield. Yield monitor data indicated penalties only within …

Genetic Control Of Morphometric Diversity In The Maize Shoot Apical Meristem, Samuel Leiboff, Xianran Li, Heng-Cheng Hu, Natalie Todt, Jinliang Yang, Xiao Li, Xiaoqing Yu, Gary J. Muehlbauer, Marja C.P. Timmermans, Jianming Yu, Patrick S. Schnable, Michael J. Scanlon

Department of Agronomy and Horticulture: Faculty Publications

The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about SAM morphological variation in natural populations. Here we report the use of high-throughput image processing to capture rich SAM size variation within a diverse maize inbred panel. We demonstrate correlations between seedling SAM size and agronomically important adult traits such as flowering time, stem size and leaf node number. Combining SAM phenotypes with 1.2 million single nucleotide polymorphisms (SNPs) via genome-wide association study reveals unexpected SAM morphology candidate …

Residue Harvest Effects On Irrigated, No-Till Corn Yield And Nitrogen Response, Charles S. Wortmann, Charles A. Shapiro, Marty R. Schmer

Department of Agronomy and Horticulture: Faculty Publications

Crop residue harvest occurs on about 40% of Nebraska’s 3,700,000 ha of corn (Zea mays L.) land, primarily for feeding of beef cattle. Immobilization of applied N is expected to be less with residue harvest due to reduced microbial activity for digestion of high C/N ratio organic material. Residue reduction may affect subsequent crop yield and response to applied N. Field research was conducted at three locations over 2 yr in eastern Nebraska for irrigated, no-till corn following corn to determine residue harvest effects on yield and the economically optimal nitrogen rate (EONR). Study sites had deep silt loam …

Evidence Of Field-Evolved Resistance To Bifenthrin In Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Populations In Western Nebraska And Kansas, Adriano E. Pereira, Haichuan Wang, Sarah N. Zukoff, Lance Meinke, B. Wade French, Blair Siegfried

Department of Entomology: Faculty Publications

Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and …

Herbicides Applied At Or Shortly After Seeding Are Effective For Weed Control In Seedling Buffalograss, Luqi Li, Matthew D. Sousek, Zachary Reicher

Department of Agronomy and Horticulture: Faculty Publications

Herbicides applied shortly after seeding of buffalograss [Buchloe dactyloides (Nutt.) Engelm.] can help reduce weed pressure and maximize establishment of buffalograss. This study evaluated 12 relatively recently developed herbicides for turf safety and weed control when applied at seeding or 0 or 2 weeks after emergence (WAE) of ‘Bowie’ or ‘Sundancer’ buffalograss. Primary weed species on the site were common purslane (Portulaca oleracea L.), redroot pigweed (Amaranthus retroflexus L.), and/or yellow foxtail [Setaria lutescens (Weigel ex Stuntz) F.T. Hubb.]. Regardless of cultivar, untreated checks had53% weed cover by 6 WAE, whereas most of the herbicide treatments resulted …

Synfind: Compiling Syntenic Regions Across Any Set Of Genomes On Demand, Haibao Tang, Matthew D. Bomhoff, Evan Briones, Liangsheng Zhang, James C. Schnable, Eric Lyons

Department of Agronomy and Horticulture: Faculty Publications

The identification of conserved syntenic regions enables discovery of predicted locations for orthologous and homeologous genes, evenwhennosuchgeneispresent.Thiscapabilitymeansthatsynteny-basedmethodsarefarmoreeffectivethansequencesimilaritybased methods in identifying true-negatives, a necessity forstudying gene loss and gene transposition. However, the identification of syntenicregionsrequirescomplexanalyseswhichmustberepeatedforpairwisecomparisonsbetweenanytwospecies.Therefore,as the number of published genomes increases, there is a growing demand for scalable, simple-to-use applications to perform comparative genomic analyses that cater to both gene family studies and genome-scale studies. We implemented SynFind, a web-based tool that addresses this need. Given one query genome, SynFind is capable of identifying conserved syntenic regions in any set of targetgenomes.SynFindiscapableofreportingper-geneinformation,usefulforresearchersstudyingspecificgenefamilies,aswellas genome-wide data sets of syntenic gene and predicted gene …

A Population Structure And Genome-Wide Association Analysis On The Usda Soybean Germplasm Collection, Nonoy Bandillo, Diego Jarquin, Qijian Song, Randall L. Nelson, Perry Cregan, James Specht, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Population structure analyses and genome-wide association studies (GWAS) conducted on crop germplasm collections provide valuable information on the frequency and distribution of alleles governing economically important traits. The value of these analyses is substantially enhanced when the accession numbers can be increased from ~1,000 to ~10,000 or more. In this research, we conducted the first comprehensive analysis of population structure on the collection of 14,000 soybean accessions [Glycine max (L.) Merr. and G. soja Siebold & Zucc.] using a 50KSNP chip. Accessions originating from Japan were relatively homogenous and distinct from the Korean accessions. As a whole, both Japanese and …

Enhancing The Effectiveness Of Biological Control Programs Of Invasive Species By Utilizing An Integrated Pest Management Approach, Invasive Species Advisory Committee

National Invasive Species Council

Conclusion

Biological control has been shown on many occasions to be the most cost-effective invasive species management tool available. However, integrating biological control projects with the full breadth of other integrated pest management (IPM) tools, expanding post-re- lease monitoring to maximize efficacy, adaptive management, and incorporating new and innovative ecological, and genetic technologies may provide private and public land managers greater opportunities for long-term success in suppression of established invasive species.

Pengaruh Keberadaan Gulma (Ageratum Conyzoides Dan Boreria Alata) Terhadap Pertumbuhan Dan Hasil Tiga Ukuran Varietas Kedelai (Glycine Max L. Merr) Pada Percobaan Pot Bertingkat, Abdul Karim Kilkoda, Tati Nurmala, Dedi Widayat

Jurnal Kultivasi

Percobaan dilaksanakan di rumah kaca Fakultas Pertanian Universitas Padjadjaran, Jati-nangor, Kabupaten Sumedang, Jawa Barat. Tujuan dari penelitian ini adalah untuk menguji eksudat akar yang terdapat pada gulma Ageratum conyzoides dan Borreria alata terhadap pertumbuhan dan hasil tanaman kedelai. Percobaan dilakukan mulai dari bulan Oktober 2014 sampai dengan bulan Desember 2014 dengan menggunakan rancangan acak kelompok pola faktorial. Faktor perlakuan pertama adalah jenis gulma terdiri dari 3 taraf antara lain gulma A. Conyzoides, gulma B. alata dan tanpa gulma (kontrol). Perlakuan kedua adalah ukuran benih varietas kedelai yang terdiri dari 3 taraf. Total kombinasi perlakuan adalah 9 kombinasi perlakuan, dengan 3 …