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Development, Isolation And Characterisation Of A New Non-Virulent Mimosine-Degrading Klebsiella Pneumoniae Strain From The Rumen Liquor Of German Steers By Using Ibt-Goettinger Bioreactor, Aung Aung, U. Ter Meulen, F. Gessler, H. Böhnel

IGC Proceedings (1977-2023)

The tropical legume Leucaena leucocephala (leucaena) has many uses, including: a potential source of firewood and timber; for soil erosion control (Dijkman 1950); to provide shade; to enhance soil fertility; and as a nutritious forage for animal feed (Ruskin 1977). It is widely used as forage for cattle in tropical agriculture (Shelton 1998). In Myanmar, leucaena is used as a protein source in urea-molasses multi-nutrient blocks for ruminants (Ni Ni Maw et al. 2004). However, the use of leucaena as ruminant feed is not without problems, because it contains mimosine, a toxic anti-nutritional factor limiting its use as animal …

Brs Paiaguás: A New Brachiaria (Urochloa) Cultivar For Tropical Pastures In Brazil, Cacilda B. Valle, Valéria P. B. Euclides, Denise B. Montagner, José R. Valério, Celso D. Fernandes, Manuel C. M. Macedo, Jaqueline R. Verzignassi, Luis A. Machado

IGC Proceedings (1977-2023)

Approximately half the world’s beef is produced in the tropics and subtropics, almost exclusively on pasture. While the world production of beef and veal increased 91% over the past 40 years, the increase in the tropics was 200%. Brazil has today the largest commercial cattle herd in the world (about 190 M head) and is the world's largest exporter of beef. The area of cultivated pasture increased from 30 to 100 Mha between 1970 and 1995 (IBGE 2006). This area has now stabilised or decreased despite the increase in beef production, which reflects the gain in area productivity. Part of …

Selecting New Brachiaria Humidicola Hybrids For Western Brazilian Amazon, Giselle M. L. De Assis, Cacilda B. Valle, Carlos M. S. De Andrade, Judson F. Valentim

IGC Proceedings (1977-2023)

Brachiaria humidicola is a perennial and stoloniferous forage grass, with excellent adaptation to infertile soils and waterlogging. Producers in northern Brazil have become interested in this species, especially after the degradation of large areas of lowly permeable soils sown with B. brizantha cv. Marandu, which is highly intolerant of soil waterlogging (Andrade and Valentim 2006). However, since only 3 cultivars of B. humidicola are registered in Brazil (Mapa 2013), there is a need to develop new cultivars adapted to the range of environmental conditions experienced. Recently, Embrapa Beef Cattle obtained intraspecific hybrids of B. humidicola that need to be evaluated …

Brs Mandobi: A New Forage Peanut Cultivar Propagated By Seeds For The Tropics, Giselle M. L. De Assis, Judson F. Valentim, Carlos M. S. De Andrade

IGC Proceedings (1977-2023)

Forage peanut (Arachis pintoi) is a forage legume of relatively recent use in beef and dairy cattle feeding. Although it is native exclusively to Brazil, the first cultivar, Amarillo, was released in Australia in 1987. Forage peanut has a great number of favourable attributes for establishing grass-legume mixtures, which guarantee the persistence and high yields of high quality forage in cultivated pastures. Therefore, studies with this species have intensified in many tropical regions of the world (Assis and Valentim 2009). In the state of Acre, western Brazilian Amazon, 137,000 ha are cultivated with forage peanut, cv. Belmonte, benefiting …

Urinary Excretion Of Mimosine Derivatives By Cows With And Without Experience In Consumption Of Leucaena Leucocephala, N. E. Ruz-Ruiz, Eduardo G. Briceño-Poot, A. J. Ayala-Burgos, C. F. Aguilar-Pérez, Francisco J. Solorio-Sánchez, Luis Ramírez-Avilés, Juan C. Ku-Vera

IGC Proceedings (1977-2023)

Leucaena leucocephala is a leguminous tree widely distributed in the tropical regions of the world. In Mexico, it has been incorporated into silvopastoral systems and is highly regarded, owing to its high content of crude protein. Nonetheless, L. leucocephala contains secondary metab-olites, such as mimosine, a non-protein free amino acid, which may induce toxic effects in unadapted ruminants that consume the forage (Hammond 1995). Although Synergistes jonesii, an anaerobic bacterium, has the ability to degrade 3,4-DHP and 2,3-DHP to non-toxic compounds (Allison et al. 1992), in Mexico its presence has not yet been confirmed. Recent work has suggested …

Urinary Excretion Of Mimosine Metabolites By Hair Sheep Fed Foliage Of Leucaena Leucocephala, L. M. Contreras-Hernández, N. E. Ruz-Ruiz, Eduardo G. Briceño-Poot, Luis Ramírez-Avilés, A. J. Ayala-Burgos, C. F. Pérez-Aguilar, Francisco J. Solorio-Sánchez, Juan C. Ku-Vera

IGC Proceedings (1977-2023)

Leucaena leucocephala is an adapted legume widely distributed in the tropical regions of Mexico. The high crude protein content of leucaena leaves renders it appropriate for ruminant feeding under commercial conditions. However, the foliage contains the non-protein amino acid mimosine, which, if consumed in high amounts, may induce toxicity in animals which have not previously consumed the legume or without microorganisms capable of degrading mimosine and its derivatives 2,3-DHP (dihydroxypyridine) and 3,4-DHP (Hammond 1995, Palmer et al. 2010, Dalzell et al. 2012). Barros-Rodríguez et al. (2012) found that dry matter intake and weight gain were reduced when …

Herbage Accumulation, Nutritive Value And Persistence Of Mulato Ii In Florida, Joao M. B. Vendramini, Lynn E. Sollenberger, Graham C. Lamb, Maria L. Silveira

IGC Proceedings (1977-2023)

Grasses in the Brachiaria genus are the most widely grown forages in tropical America, occupying over 80 Mha (Boddey et al. 2004). Mulato II is apomictic and a vigorous, semi-erect cultivar resulting from 3 generations of crosses including original crosses between ruzigrass and signal-grass (cv. Basilisk, apomictic tetrapliod). According to Peters et al. (2003), Mulato produced 25% more herbage mass than palisadegrass (Brachiaria brizantha) and koroni-viagrass (Brachiaria humidicola) under similar management practices. Although Mulato II shows promise as a forage in tropical regions, herbage accumulation and persistence in subtropical areas is unknown. This publication …

Agronomic And Nutritional Evaluation Of Intraspecific Crosses In Brachiaria Decumbens, Simony A. Mendonça, Sanzio C. L. Barrios, Ulisses J. De Figueiredo, Geovani F. Alves, Cacilda B. Valle

IGC Proceedings (1977-2023)

Brachiaria decumbens cv. Basilisk is the single most important forage grass used for pastures in the tropics. This cultivar has exceptional adaptation to acid soils, vigorous growth, ease of establishment, and good forage value throughout the year, but these favourable characteristics are counteracted by its susceptibility to insect pests such as spittlebug. Breeding to produce improved cultivars within this species was impossible until 2009 due to the lack of compatible sexual ecotypes. With the success of somatic chromosome duplication of sexually reproducing diploid plants of B. decumbens (Simioni and Valle 2009), intraspecific crosses with natural apomictic tetraploid accessions were finally …

Reciprocal Recurrent Selection In The Breeding Of Brachiaria Decumbens, Sanzio C. L. Barrios, Cacilda B. Valle, Geovani F. Alves, Rosangela M. Simeão, Liana Jank

IGC Proceedings (1977-2023)

Pastures of Brachiaria decumbens cv. Basilisk radically changed the scenario of livestock production in central Brazil in the early 1970s and in fact, promoted the development of this vast region. However, despite the reasonable biomass yields and nutritional value when grown on these tropical acid soils, its susceptibility to grassland spittlebugs has limited its use. The breeding of B. decumbens in Brazil has been restricted to interspecific crosses using cv. Basilisk as a pollen donor due to the lack of compatible sexual ecotypes within this species. Recently, the successful chromosome duplication of a sexually reproducing diploid accession produced 3 successful …

Status Of Animal Feed Resources In Rwanda, M. Mutimura, A. B. Lussa, J. Mutabazi, C. B. Myambi, R. A. Cyamweshi, C. Ebong

IGC Proceedings (1977-2023)

Animal feed resources remain a major constraint for livestock development in tropical Africa. In Rwanda, grazing lands are shrinking sharply because crop cultivation is progressively encroaching on grazing areas with increasing human pressure (Mutimura and Everson 2011). Therefore, over 60% of households cultivating less than 0.7 ha, and owning livestock, practise zero-grazing, where farmers cut-and-carry forage and crop residues to feed animals that are kept exclusively under sheds (MINAGRI 2009). In general, the main feed for dairy cattle under a zero-grazing system is Napier grass (Pennisetum purpureum). For more than a decade, efforts to improve the feed resource …

Brachiaria Hybrids: Potential, Forage Use And Seed Yield, Esteban A. Pizarro, Michael D. Hare, Mpenzi Mutimura, Changjun Bai

IGC Proceedings (1977-2023)

A brachiaria breeding program initiated in 1988 at CIAT (Centro Internacional de Agricultura Tropical) combined desirable attributes found in accessions of Brachiaria brizantha and B. decumbens. Three apomictic hybrids have been released (cvs. Mulato, Mulato II and Cayman). Mulato showed agronomic potential but seed yields were low. Trials in Central America demonstrated the superiority of Mulato II, a vigorous grass with very deep and branched roots, giving it excellent drought resistance in the Brazilian Cerrado and Mexico. Mulato II has excellent nutritional value. Following trials in Mexico and Thailand, evaluating 155 new hybrids for 7 years, cv. Cayman was …

Molecular Genetic Variability, Population Structure And Mating System In Tropical Forages, Melissa Garcia, Bianca B. Z. Vigna, Adna C. B. Sousa, Letícia Jungmann, Fernanda W. Cidade, Guilherme Toledo-Silva, Patricia M. Francisco, Lucimara Chiari, Marcelo A. Carvalho, Claudio T. Karia, Fabio G. Faleiro, Rodolfo Godoy, M. Dall’Agnol, Sueli S. Pagliarini, Francisco H. D. Souza, Tatiana T. Souza-Chies, Liana Jank, Rosangela M. S. Resende, Cacilda B. Valle, Maria I. Zucchi, Anete P. Souza

IGC Proceedings (1977-2023)

Microsatellite (SSR) markers were developed for the following tropical forage species, using accessions available from the plant genetic resources (PGR) collections held by EMBRAPA (Brazilian Agricultural Research Corporation): Brachiaria brizantha, B. humidicola, Panicum maximum, Paspalum spp., Stylosanthes capitata, S. guianensis, S. macrocephala, Calopogonium mucunoides and Centrosema spp. The markers were used to analyse population structure and genetic diversity, evolution and origin of the genetic variability in the centre of origin, mating systems and genetic resources in EMBRAPA’s germplasm bank. The results shed light on the amount of genetic variation within and between populations, revealed …

Leucaena Toxicity: A New Perspective On The Most Widely Used Forage Tree Legume, Michael J. Halliday, Jagadish Padmanabha, Chris S. Mcsweeney, Graham L. Kerven, H. Max Shelton

IGC Proceedings (1977-2023)

The tree legume Leucaena leucocephala (leucaena) is a high quality ruminant feed, vitally important for livestock production in the tropics despite the presence of mimosine in the leaves. This toxic non-protein amino acid has the potential to limit productivity and adversely affect the health of animals. The discovery and subsequent distribution in Australia of the ruminal bacterium Synergistes jonesii as an oral inoculum was shown in the 1980s to overcome these toxic effects. However, recent surveys of the status of toxicity worldwide; improved understanding of the chemistry and mode of action of the toxins; new techniques for molecular sequencing; and …

Global Impacts From Improved Tropical Forages: A Meta-Analysis Revealing Overlooked Benefits And Costs, Evolving Values And New Priorities, Douglas S. White, Michael Peters, Peter Horne

IGC Proceedings (1977-2023)

The wider use and improved performance of planted tropical forages can substantially change social, economic and environmental landscapes. By reviewing impact-related studies published in the past two decades, this paper shows how evolving development priorities have influenced the types of impacts being documented. A meta-analysis was used to examine 98 studies according to: (1) breadth of reported effects, as related to development goals of social equity, economic growth and environmental sustainability; (2) extent of effects, ranging from intermediate to longer-term impacts; and (3) measurement precision (identification, description and quantification). Impacts have been assessed for fewer than half of the documented …

Response Of Axonopus Catarinensis And Arachis Pintoi To Shade Conditions, A. N. A. Pachas, E. J. Jacobo, M. C. Goldfarb, S. M. Lacorte

IGC Proceedings (1977-2023)

In the north-east of Argentina, there are more than 100,000 hectares of silvopastoral systems where trees, forages and livestock are combined with the goal to diversify income, reduce financial risk, obtain more profit and enhance environmental benefit (Cubbage et al. 2013). The rapid adoption of this production system by farmers has generated high demand for information on shade tolerant grass and legume forage species.

Axonopus catarinensis is a native grass from Itajai Vally (Brazil) that was introduced to the north-east of Argentina 10 years ago; whereas Arachis pintoi is a sub-tropical legume (also native to Brazil) adapted to acid …

The Growth Response Of Tropical And Sub-Tropical Forage Species To Increasing Salinity, Hayley E. Giles, Christopher J. Lambrides, Scott A. Dalzell, David C. Macfarlane, H. Max Shelton

IGC Proceedings (1977-2023)

There is currently a growing coal seam gas (CSG) industry in Queensland, Australia. The industry requires beneficial-use strategies to consume the significant volumes of water released during CSG extraction. Irrigation of tropical and sub-tropical forage species for beef production is one option, however coal seam (CS) water is of varying quality due to moderate to high salinity and alkalinity. The application of chemically amended CS water over time could potentially increase soil salinity, which is known to reduce plant biomass production. While there were studies of salinity tolerance of many tropical and sub-tropical forage species 30 years ago, there is …

Performance Of Some Forages Species (Festuca Arundinacea L., Chloris Gayana Var. Katambora, Lotus Corniculatus L. And Medicago Sativa L.) In Saline Soil, Süleyman Temel, Uğur Şimşek, Bilal Keskin, İbrahim H. Yılmaz

IGC Proceedings (1977-2023)

Salinity is a soil degradation process which reduces plant diversity and agricultural productivity, decreases fertility and devalues the land in regions with arid and semi-arid climate. This process inhibits water and nutrient intake of plants from soil due to changeable nitrogen percentage and/or the intensity of soluble salt concentration. When the fact that agricultural lands are limited around the world and that the need for nutrition increases incrementally is taken into consideration, it is obvious that available lands should be used more effectively. Hence, it is quite crucial to reclaim saline soil and utilize it more economically (Woods 1996).

Plantain (Plantago Lanceolata) Outperforms Chicory (Cichorium Intybus) Under Moisture Stress In Glasshouse, Lydia M. Cave, Peter D. Kemp, Paul R. Kenyon, Stephen T. Morris

IGC Proceedings (1977-2023)

Forage chicory (Cichorium intybus) and plantain (Plantago lanceolata) are now widely used throughout the world as high feed quality perennial herbage (Sanderson et al. 2003; Labreveux et al. 2006; Li et al. 2010; Golding et al. 2011; Hutton et al. 2011). Both are taprooted plants and are thus likely to confer a degree of drought tolerance through accessing water deeper in the soil profile (Kemp et al. 2010). Nie et al. (2008) reported chicory can tolerate moisture stress to a greater degree than plantain. However, overall little is known …

Uji Daya Hasil Jamur Tiram Putih (Pleurotus Ostreatus) Akibat Aplikasi Jenis Nutrisi Alternatif Dengan Pendekatan Bioklimatik Di Kabupaten Karawang, Rommy Andhika Laksono

Jurnal Kultivasi

Karawang merupakan wilayah di Jawa Barat yang sangat potensial dalam pengembangan budidaya jamur tiram putih. Salah satu usaha penerapan sistem pertanian yang berkelanjutan atau sistem pertanian yang peduli lingkungan dapat dilakukan dengan pemanfaatan nutrisi alternatif dan modifikasi bioklimatik. Penelitian ini bertujuan untuk mendapatkan jenis nutrisi alternatif terbaik yang mampu memberikan produksi jamur tiram putih tertinggi pada setiap posisi baglog. Penelitian dilakukan di kumbung percobaan Fakultas Pertanian UNSIKA, Kabupaten Karawang, dari bulan Januari sampai dengan bulan Agustus 2018. Metode penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) faktorial. Perlakuan terdiri dua faktor, yaitu jenis nutrisi alternatif dan posisi baglog. Faktor jenis …

Plant Hormones Differentially Control The Sub-Cellular Localization Of Plasma Membrane Microdomains During The Early Stage Of Soybean Nodulation, Zhenzhen Qiao, Prince Zogli, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Phytohormones regulate the mutualistic symbiotic interaction between legumes and rhizobia, nitrogen-fixing soil bacteria, notably by controlling the formation of the infection thread in the root hair (RH). At the cellular level, the formation of the infection thread is promoted by the translocation of plasma membrane microdomains at the tip of the RH. We hypothesize that phytohormones regulate the translocation of plasma membrane microdomains to regulate infection thread formation. Accordingly, we treated with hormone and hormone inhibitors transgenic soybean roots expressing fusions between the Green Fluorescent Protein (GFP) and GmFWL1 or GmFLOT2/4, two microdomain-associated proteins translocated at the tip of the …

The Effects Of Seed-Applied Fluopyram On Root Penetration And Development Of Meloidogyne Incognita On Cotton And Soybean, Tracy Hawk

Graduate Theses and Dissertations

Plant-parasitic nematodes are major pests of cotton and soybean in Arkansas, and across the Southern United States. These nematodes cause more than $3 billion worth of crop losses each year, in part due to lack of available control tactics, such as nematicides. Fluopyram has recently been registered as a seed-treatment nematicide in agronomic crops. The toxicity of fluopyram against Meloidogyne incognita infection has been reported, however, information on root protection provided by fluopyram against Meloidogyne incognita is lacking. The first objective of this research was to evaluate the effect seed-applied fluopyram had on nematode development, root galling, and reproduction on …

Use Of Aerial Imagery And Novel Experimental Design To Determine The Distribution Of Foliar Diseases On Soybean And Improve Efficiency Of Product Testing, Mckayla Patterson

Graduate Theses and Dissertations

Soybeans are grown on approximately 1.3 million ha in Arkansas generating an estimated $1.7 billion annually. Foliar diseases on soybean can result in economic losses. Growers spend significant time and money on disease scouting via crop consultants and often (subsequent) fungicide applications. Fungicide trials are often arranged in small plots designs. In these scenarios, spatial variability of foliar disease is minimized. While it is advantageous to minimize variance outside of treatment differences, another limitation with many small plot trials is ample disease pressure or having only lower severity. Within a commercial production field, soil types and disease severities vary. Logically, …

Comparison Of Morphological Traits In Cowpea Pod Length, Seed Number Per Pod, Seed Weight, Seed Color And Seed Density In Usda Germplasm Accessions And Arkansas Breeding Lines, Sora Imamura

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

The objective of the study was to compare and correlate the Arkansas cowpea breeding lines to the USDA cowpea germplasm accessions on the following morphological traits: seed color, pod length, number of seeds per pod, 100 seed weight, and seed density. The seed density is defined as the weight of seed in gram per centimeter of pod length. Three hundred seventeen cowpea genotypes were used in this experiment. Out of the 317, 285 germplasm accessions were from the USDA GRIN and 32 lines were from the Arkansas breeding program.

T-test between Arkansas breeding lines and USDA germplasm accessions were done …

Effects Of Different Water And Nitrogen Regimens On Yield Of Winter Wheat Produced In Nebraska, Joseph Emory Davis

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Wheat is the 3rd most prominent crop in the United States and approximately 50% is exported annually. Nebraska wheat production is 11^th in the country, and it plays a major role in the state's agricultural economy, especially in western Nebraska. Generally, wheat is grown under dryland conditions and the region grows much more wheat on unirrigated land than it does on irrigated. However, deficit irrigation has shown great value in producing high yielding wheat with much less water than needed for other crops. Finding new ways to leverage irrigation in wheat production may help address the need to produce …

A Survey Of Soil Properties Affecting Vegetation Establishment Along Nebraska Highways, Shad D. Mills

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Vegetation along roadsides is important to prevent soil erosion, provide habitat and filter water running off the road. Along some highways in Nebraska vegetation does not readily establish and persist. It is thought that sodium and bulk density issues are the driving factor behind the lack of vegetation. After a construction project, the shoulder is seeded into the compacted soil, and salts can accumulate in the soil due to deicing agents being used during the winter. The purpose of our study was to determine if the bulk density and sodium are the driving factors of the vegetation cover. We also …

Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda E. Shine

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nutrient inputs from cattle dung are crucial drivers of nutrient cycling processes in grazed ecosystems. These inputs are important both spatially and temporally and are affected by variables such as grazing strategy, water location, and the nutritional profile of forage being grazed. Past research has attempted to map dung deposition patterns in order to more accurately estimate nutrient input, but the large spatial extent of a typical pasture and the tedious nature of identifying and mapping individual dung pats has prohibited the development of a time- and cost-effective methodology. The first objective of this research was to develop and validate …

An Evaluation Of Hybrid Traits, Yield, And Major Qtl Effect On Heterosis In Hybrid Soft Red Winter Wheat, Zachary James Winn

Graduate Theses and Dissertations

Wheat (Triticum aestivum L.) is a self-pollinating species that is most often bred as a recombinant inbred line. When two inbred individuals are crossed, they produce progeny which experience heterosis; heterosis is the increased robustness experienced due to the reintroduction of heterozygosity. Hybrid wheat may be produced through the use of chemical hybridization agents, yet the structure of wheat flowers decreases the efficiency of outcrossing. The objectives of this study were to: assess the amount of heterosis experienced by a population of hybrid wheat and observe the effect of major gene loci on yield and yield components, phenotype anthers extruded …

Analyzing Multigene Stacking And Genome Editing Strategies In Rice, Bhuvan Pathak

Graduate Theses and Dissertations

Crop improvement through biotechnology is an integrated effort, incorporating multiple approaches like integration of genes, editing of native genes, and removal of selection marker genes. Before streamlining the protocols, the efficiency and feasibility of the individual approach and their components must be tested. This study evaluated following approaches: 1) stacking an array of genes into a single locus by site-specific integration via Cre-lox recombination in rice, 2) determining the efficiency of I-SceI and the CCR5-ZFN in the targeted excisions of gene fragments in rice and Arabidopsis, and 3) determining the efficiency of CRISPR/Cas9 in generating targeted mutations for genome editing …

Integration Of Cover Crops Into Midwest Corn-Soybean Cropping Systems And Potential For Weed Suppression, Joshua S. Wehrbein

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Cover crops have potential to provide benefits to agricultural systems, such as improved soil productivity, nutrient scavenging, weed suppression, and livestock forage. There are several challenges associated with cover crop integration into traditional Midwest corn-soybean cropping systems. One of these challenges is timely establishment in the fall, which is limited by the relatively late harvest of corn and soybean. Cover crop effectiveness is related to the amount of biomass produced, thus maximizing the growth period in the fall is desired. To address this challenge, we evaluated the potential to utilize early-season soybean maturity groups (MGs) to allow for earlier soybean …

Benchmarking On-Farm Maize Nitrogen Balance In The Western U. S. Corn Belt, Fatima Amor Tenorio

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A nitrogen (N) balance, calculated as the difference between N inputs and grain-N removal, provides an estimate of the potential N losses. We used N balance with other N-related metrics (partial factor productivity for N inputs, and yield-scaled N balance), to benchmark maize yields in relation with N input use in the US Corn Belt. We first used experimental data on grain-N concentration (GNC) to assess variation in this parameter due to biophysical and management factors. Subsequently, we used N balance and N-related metrics to benchmark yields in relation with N inputs in irrigated and rainfed fields in Nebraska using …