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Genetic And Molecular Characterization Of Submergence Response Identifies Subtol6 As A Major Submergence Tolerance Locus In Maize, Malachy T. Campbell, Christopher A. Proctor, Yongchao Dou, Aaron J. Schmitz, Piyaporn Phansak, Greg R. Kruger, Chi Zhang, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Maize is highly sensitive to short term flooding and submergence. Early season flooding reduces germination, survival and growth rate of maize seedlings. We aimed to discover genetic variation for submergence tolerance in maize and elucidate the genetic basis of submergence tolerance through transcriptional profiling and linkage analysis of contrasting genotypes. A diverse set of maize nested association mapping (NAM) founder lines were screened, and two highly tolerant (Mo18Wand M162W) and sensitive (B97 and B73) genotypes were identified. Tolerant lines exhibited delayed senescence and lower oxidative stress levels compared to sensitive lines. Transcriptome analysis was performed on these inbreds to provide …

Qtl For Seed Protein And Amino Acids In The Benning × Danbaekkong Soybean Population, C. V. Warrington, H. Abdel‑Haleem, D. L. Hyten, P. B. Cregan, J. H. Orf, A. S. Killam, N. Bajjalieh, Z. Li, H. R. Boerma

Department of Agronomy and Horticulture: Faculty Publications

Soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. As a sole dietary source of protein, soybean is deficient in the amino acids lysine (Lys), threonine (Thr), methionine (Met), and cysteine (Cys). Increasing these amino acids would benefit the feed industry. The objective of the present study was to identify quantitative trait loci (QTL) associated with crude protein (cp) and amino acids in the ‘Benning’ × ‘Danbaekkong’ population. The population was grown in five southern USA environments. Amino acid concentrations as a fraction of cp (Lys/cp, Thr/cp, …

Using A Simple Leaf Color Chart To Estimate Leaf And Canopy Chlorophyll A Content In Maize (Zea Mays), Anthony Nguy-Robertson, Yi Peng, Timothy Arkebauer, David Scoby, James Schepers, Anatoly Gitelson

Department of Agronomy and Horticulture: Faculty Publications

This study utilized a leaf color chart (LCC) to characterize the variation in leaf chlorophyll and estimate canopy chlorophyll in maize (Zea mays). The LCC consisted of four levels of greenness and was used to sort maize leaves in 2011 for three fields near Mead, Nebraska, USA. Leaf chlorophyll content for each color chart class was determined using two leaf-level sensors. The variation within each LCC class was reasonable (CV < 56%). The darkest color class predominated and indicated adequate fertilization rates using a SPAD. Canopy chlorophyll content was estimated using destructively measured leaf area index (LAI) and the LCC. This approach was verified with a method utilizing canopy reflectance collected by both satellite imagery and a four-band radiometer. The error between the two methods was reasonable (RMSE = 0.55-0.88 g m^-2; CV = 25.6-50.4%), indicating that both leaf and canopy chlorophyll can be estimated cheaply without a wet lab or field-based sensors.

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, …

Tonoplast Sugar Transporters (Sbtsts) Putatively Control Sucrose Accumulation In Sweet Sorghum Stems, Saadia Bihmidine, Benjamin T. Julius, Ismail M. Dweikat, David M. Braun

Department of Agronomy and Horticulture: Faculty Publications

Carbohydrates are differentially partitioned in sweet versus grain sorghums. While the latter preferentially accumulate starch in the grain, the former primarily store large amounts of sucrose in the stem. Previous work determined that neither sucrose metabolizing enzymes nor changes in Sucrose transporter (SUT) gene expression accounted for the carbohydrate partitioning differences. Recently, 2 additional classes of sucrose transport proteins, Tonoplast Sugar Transporters (TSTs) and SWEETs, were identified; thus, we examined whether their expression tracked sucrose accumulation in sweet sorghum stems. We determined 2 TSTs were differentially expressed in sweet vs. grain sorghum stems, likely underlying the massive difference in sucrose …

Selection For Silage Yield And Composition Did Not Affect Genomic Diversity Within The Wisconsin Quality Synthetic Maize Population, Aaron Lorenz, Timothy M. Beissinger, Renato Rodrigues Silva, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Maize silage is forage of high quality and yield, and represents the second most important use of maize in the United States. The Wisconsin Quality Synthetic (WQS) maize population has undergone five cycles of recurrent selection for silage yield and composition, resulting in a genetically improved population. The application of high-density molecular markers allows breeders and geneticists to identify important loci through association analysis and selection mapping, as well as to monitor changes in the distribution of genetic diversity across the genome. The objectives of this study were to identify loci controlling variation for maize silage traits through association analysis …

Overexpression Of The Transporters Atzip1 And Atmtp1 In Cassava Changes Zinc Accumulation And Partitioning, Eliana Gaitán-Solis, Nigel J. Taylor, Dimuth Siritunga, William Stevens, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Zinc deficiency in humans is a serious problem worldwide with an estimated one third of populations at risk for insufficient zinc in diet, which leads to impairment of cognitive abilities and immune system function. The goal of this research was to increase the bioavailable zinc in the edible portion of cassava roots to improve the overall zinc nutrition of populations that rely on cassava as a dietary staple. To increase zinc concentrations, two Arabidopsis thaliana genes coding for ZIP1 and MTP1 were overexpressed with a tuber-specific or constitutive promoter. Eighteen transgenic events from four constructs, out of a total of …

Urea Formulations On The Productivity Of Bermudagrass And Bermudagrass-White Clover Pastures, Caitlin Timberlake

Theses and Dissertations--Plant and Soil Sciences

Bermudagrass (Cynodon dactylon L.) is a perennial warm-season grass that is very responsive to nitrogen (N) fertilization. Excessive N applications have negative environmental consequences and make maintaining mixed swards difficult. This study determined the effects of enhanced efficiency (EE) N fertilizers and fertilizer rate on bermudagrass yields, nutritive values, and white clover persistence. Nitrogen sources included urea, urea formulated with Agrotain^® (U+A), urea with Agrotain^® and dicyandiamide (SuperU), a polymer-coated urea (ESN), ESN+urea (75% ESN, 25% urea), and methylene urea (MU). In the urea formulation trial, SuperU and U+A maximized forage yields at lower N rates. The …

Focus Paddocks Trial Report - Benchmarked Result Summary 2010 - 14, Martin Harries, Geoff Anderson

Grain and other field crops published reports

This report was co-funded by GRDC through project no. DAW00213 - Putting the Focus on Profitable Crop and Pasture Sequences in WA.

Crop and pasture sequences in south-western Australia have changed in the past decade. These survey results from the Focus Paddock project provide data to help growers employ profitable and sustainable crop and pasture sequences. The project compiled information on the nature, suitability and productivity of crop and pasture sequences from 184 paddocks over five years. The results of the analyses of chemical and physical attributes of the soil, weed counts and disease management are presented here.

The survey …

Growing South Dakota (Winter 2015), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2014 SDSU Agricultural Experiment Station Annual Report.

[Page] 2 AES Director’s Message
[Page] 3 Better Beans: Innovative Research Seeks To Increase Soybean’s Nitrogen-Fixing Ability
[Page] 6 Bioprocessing Adds Value: Researchers Turn Agricultural Residue Into Energy Storage Material
[Page] 8 Protecting Water Quality: Conservation Practices Minimize Erosion, Surface Runoff
[Page] 10 Balancing Ag & Wildlife: Cover Crop Food Plots Help Manage Deer Population
[Page] 11 Industry Insight: Beef Consumers Get Information From Websites, Social Media
[Page] 12 Evaluating Efficiency: Swine Nutritional Studies Underway; New Facility Will Allow For Expanded Research
[Page] 14 SDSU Hosts National Ag Leaders …

Addressing Food Security: Saba Banana And Rootcrops As Alternative Food Staples To Rice In Quezon Province, Philippines, Isabelita M. Pabuayon, Blanquita R. Pantoja, Anselma C. Manila, Mac Lorenz C. Santos

Journal of Public Affairs and Development

The Philippines considers rice self-sufficiency as vital to food security; hence, possible alternative food staples are being identified in case of persistent shortfalls in domestic rice production. This paper explores the potential of Saba (cardava) banana, sweet potato, potato, yam, and cassava as alternative staples for the local communities in the Quezon province, Philippines, which grow all these crops. Socioeconomic surveys of 375 farmers, 82 traders, and 625 consumers were conducted in 2013 but covered crop year 2012. Results showed that Saba banana, sweet potato, and cassava had the highest demand among the five crops based on per capita consumption. …

First Year Biomass Production Following Application Of Various Size Strip Thinnings In A Young Hardwood Stand In West Virginia., Ashlee Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Strip thinning can reduce stand density to promote better growth of residual trees and has potential to create a woody bioenergy feedstock in addition to enhancing and maintaining early successional habitat for wildlife. However, little research has been conducted on strip thinning within young hardwood stands to determine if these are viable attributes of strip thinning. The goal of this study was to assess the first year biomass production after strip thinning among three different cut strip widths (8 ft, 12 ft, and 16 ft) in a 22-year-old mixed mesophytic hardwood stand. Woody biomass production from stump sprouts and true …

Operational Policy Needs For Organic Agriculture Expansion In The Philippines: Focus On Vegetables, Agnes C. Rola, Blanquita R. Pantoja, Agnes R. Chupungco, Miriam R. Nguyen, Jaine C. Reyes, Guinevere T. Madlangbayan, Macrina G. Umali, Susan S. Guiaya, Eldy Z. Martinez, Gerdino G. Badayos

Journal of Public Affairs and Development

In 2010, the Philippines passed a law (Republic Act No. 10068) to support the expansion of organic agriculture (OA) in the country, with a goal of 5 percent of total production area being devoted to this technology. Available data show that in 2012, only about 0.7 percent of the agricultural land area in the country was devoted to OA production. While several other administrative orders supported the law, there seems to be a need to understand the processes to operationalize the said law.

This paper analyzed the production, marketing, and consumption issues surrounding the organic vegetable industry to come up …

Increasing Student Interest In Poultry Science Careers Through 4-H, Peggy Lynn Armstrong

Walden Dissertations and Doctoral Studies

The demand for poultry science graduates to fill available positions in the poultry industry continues to increase. At the same time, there are not enough graduates to fill the positions. The purpose of this study was to investigate the implementation of the poultry curriculum in the 4-H program and student recruitment through the 4-H program into the Poultry Science Department. The targeted populations were 4-H leaders who implement the poultry curriculum and university students who were currently studying poultry. Career development theories suggest that previous experiences during adolescence can be a major influence in future vocation choice. A mixed methods …

How To Grill Sweet Corn, Shawn Olsen

All Current Publications

This fact sheet describes techniques for grilling sweet corn, including selection criteria, and preparation options.

Harvest And Storage Of Vegetables And Fruits, Shawn Olsen, Dan Drost, Teresa Hunsaker

All Current Publications

This 7-page fact sheet describes when and how to harvest fruits and vegetables and gives different options for storage for later use.

Three Essays On Food Demand, Land Use Intensity, And Food Crop Portfolio Choice Under Weather Risk, Aditya Raj Khanal

LSU Doctoral Dissertations

Food security and adaptation to climate change has been an important research and development agenda in most of the development organizations and in the national policy of developing countries. The most vulnerable population from climate change and food security problems are rural and agricultural households in the developing and low income countries. Weather and climatic factors and food security has a direct link with poverty because a large majority of poor live in rural areas and depend on agriculture for their livelihoods. The primary focus of this dissertation is to analyze consumption and production aspects of the rural agricultural households. …

Estimating Desirable Cattle Traits Using Latent Class And Mixed Logit Models: A Choice Modeling Application To The U.S. Grass-Fed Beef Industry, Isaac Sitienei

LSU Doctoral Dissertations

This study examines the preferences for cattle traits using mixed logit and latent class models. Choice experiment data from a 2013 mail survey of 1,052 U.S. grass-fed beef producers were used. The findings indicate that grass-fed beef producers generally preferred lower-priced, heavy animals that were small-to-medium framed and easy to handle for grass-finishing. Black animals that were retained from their own calves were preferred. Relative to intact males, steers and heifers were preferred. Except for the estimated parameter for the weight attribute, the standard deviations for the temperament, body frame, source, color, gender, and price attribute levels were significant at …

Metabolic Engineering Of Oilseed Crops To Produce High Levels Of Novel Acetyl Glyceride Oils With Reduced Viscosity, Freezing Point And Calorific Value, Jinjie Liu, Adam Rice, Kathleen Mcglew, Vincent Shaw, Hyunwoo Park, Thomas E. Clemente, Mike Pollard, John Ohlrogge, Timothy P. Durrett

Department of Agronomy and Horticulture: Faculty Publications

Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2- diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina and soybean …

Index-Based Insurance And Risk Management Among Nomadic Mongolian Herders , Kelsey Larson

Independent Study Project (ISP) Collection

Mongolian herders must contend with the risk of dzuds, harsh winters that can kill large numbers of livestock. To do so, they use a mixture of formal financial tools and traditional risk mitigation techniques. This paper is a study of the interaction between the Mongolian Index-based Livestock Insurance Program and traditional informal risk mitigation techniques. The researcher interviews herders in Bulgan soum, Arhangai aimag and Galuut soum, Bayanhongor aimag to compare the IBLI program’s impact in a community that has had IBLI since 2006 and one that only received IBLI in 2012. This study finds that insurance purchase is positively …

Effects Of Stocking Rate On Forage Nutrient Composition Of Nebraska Sandhills Upland Range When Grazed In Early Summer And The Effects Of Grazing On Nebraska Sandhills Meadow Forage Nutrient Composition, Jared Vern Judy

Department of Animal Science: Dissertations, Theses, and Student Research

The objectives of this research were to 1) evaluate the effects of stocking rate on forage nutrient quality 2) quantify the relative proportions of current vs. previous year growth being consumed in early summer upland range pastures and 3) determine how grazing effects forage nutrient quality in subirrigated meadows in the Nebraska Sandhills. Experiment 1 was a two year study conducted on the experimental upland range paddocks at Gudmundsen Sandhills Laboratory. Twelve 2-hectare paddocks were assigned one of three treatments stocked at 0 (control), 0.57 (light), and 0.85 (heavy) AUM/ha. Ten 0.25 m² quadrats were clipped per paddock during …

Proper Handling And Transportation Of Eggs For Sale At Kentucky Farmers’ Markets, Anthony J. Pescatore, Jacquie Jacob

Agriculture and Natural Resources Publications

Regardless of the number of eggs produced, and whether the eggs are for home use or sale, careful egg handling is very important. The United States Food and Drug Administration (FDA) lists eggs as a potentially hazardous food, therefore careful handling is important. In Kentucky, if you sell more than 60 dozen eggs a week you need to purchase a retail license. The same holds true if you want to sell your eggs to a retail store to be re-sold or to a distributor that will sell them for you. If you produce fewer than 60 dozen eggs a week …

Sheep Breeding: Heritability, Ebvs, Epds And The Nsip, Debra K. Aaron

Agriculture and Natural Resources Publications

Genetic improvement in a flock depends on the producer’s ability to select breeding sheep that are genetically superior for traits of economic importance. This is complicated by the fact that an animal’s own performance is not always a true indicator of its genetic potential as a parent.

An Introduction To Sheep, Debra K. Aaron, Donald G. Ely

Agriculture and Natural Resources Publications

The information in this fact sheet was developed to provide a quick reference to the most frequently asked questions about sheep and sheep production.

Basic Sheep Genetics, Debra K. Aaron

Agriculture and Natural Resources Publications

Genetics is the science of heredity. It seeks to explain differences and similarities exhibited by related individuals. The application of genetics to livestock improvement is known as animal breeding. The objective of this fact sheet is to provide a refresher course on basic genetics and to show how knowledge of genetics can be used to improve sheep production.

Keeping And Using Flock Performance Records, Debra K. Aaron

Agriculture and Natural Resources Publications

Performance records serve as the cornerstone of any good livestock management program. Unfortunately, the task of collecting, maintaining and using performance records is the one area of livestock production in general that gets the least attention. This fact sheet provides ten reasons why all sheep producers need to keep performance records on their flocks. Then, some ways of maintaining and using those records are discussed.

So You Want To Produce Your Own Eggs?, Jacquie Jacob, Anthony J. Pescatore

Agriculture and Natural Resources Publications

Backyard chicken flocks are becoming popular throughout the country in urban, suburban and rural communities. Preparation is essential for a successful backyard flock.

An Almost Ideal Demand System Analysis Of Orange And Grapefruit Beverage Consumption In The United States, Catrice Taylor

All Theses

This thesis estimates a demand system for natural fruit juices and flavored citrus drinks. Consumption and price data from the Florida Department of Citrus is used to estimate a Linear Approximate Almost Ideal Demand System for natural fruit and fruit juice drink elasticities. The monthly data covers the period from October 2004 to June 2014. Elasticities show orange juice does not have a substitute among the other beverages. 100% orange juice and grapefruit juice are compliments, although other studies have found them to be substitutes.

Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt

Master's Theses

Packaging Materials account for 31% of the world’s municipal solid waste. Agencies like the Environmental Protection Agency (EPA) and the Agency for Toxic Substances and Disease Registry (ATSDR) are pushing for the increased use of recycled thermoplastic materials. Polyethylene terephthalate (PET) is a commonly recycled thermoplastic which is used to package ready-to-eat fruits and vegetables. Most recycled polyethylene terephthalate (RPET) packaging materials contain heavy metal catalysts, the most common being antimony. The recent increased use of recycled plastic materials has been suspected as the source of increased human heavy metal exposure. In this study, cadmium, chromium, nickel, lead and antimony …

Assessing Net Returns To Blueberry Production Using A Decision Support Tool, Sokha Sok

Graduate Theses and Dissertations

The purpose of this study was to develop and assess the profitability of four highbush blueberry farming systems in the south: organic field production, conventional field production, organic high tunnel production and conventional high tunnel production. Four baseline budget scenarios, one for each system, are developed for a 15 year production period. The results suggest that under expected production and price conditions for Northwest Arkansas, while all four production systems generated positive present value of net returns, the conventional field production produced the highest present value of net returns across the 15 years. The breakeven years of production were 7 …