Digital Commons Network^™

Horizontal Gene Acquisitions, Mobile Element Proliferation, And Genome Decay In The Host-Restricted Plant Pathogen Erwinia Tracheiphila, Lori R. Shapiro, Erin D. Scully, Timonthy J. Straub, Jihye Park, Andrew G. Stephenson, Gwyn A. Beattie, Mark L. Gleason, Roberto Kolter, Miguel C. Coelho, Consuelo M. De Moraes, Mark C. Mescher, Olga Zhaxybayeva

Department of Agronomy and Horticulture: Faculty Publications

Modern industrial agriculture depends on high-density cultivation of genetically similar crop plants, creating favorable conditions for the emergence of novel pathogens with increased fitness in managed compared with ecologically intact settings. Here, we present the genome sequence of six strains of the cucurbit bacterial wilt pathogen Erwinia tracheiphila (Enterobacteriaceae) isolated from infected squash plants in New York, Pennsylvania, Kentucky, and Michigan. These genomes exhibit a high proportion of recent horizontal gene acquisitions, invasion and remarkable amplification of mobile genetic elements, and pseudogenization of approximately 20% of the coding sequences. These genome attributes indicate that E. tracheiphila recently emerged as a …

Nutrients In The Nexus, Eric A. Davidson, Rachel L. Nifong, Richard B. Ferguson, Cheryl Palm, Deanna L. Osmond, Jill S. Baron

Department of Agronomy and Horticulture: Faculty Publications

Synthetic nitrogen (N) fertilizer has enabled modern agriculture to greatly improve human nutrition during the twentieth century, but it has also created unintended human health and environmental pollution challenges for the twentyfirst century. Averaged globally, about half of the fertilizer-N applied to farms is removed with the crops, while the other half remains in the soil or is lost from farmers’ fields, resulting in water and air pollution. As human population continues to grow and food security improves in the developing world, the dual development goals of producing more nutritious food with low pollution will require both technological and socioeconomic …

Alkaline Stress And Iron Deficiency Regulate Iron Uptake And Riboflavin Synthesis Gene Expression Differently In Root And Leaf Tissue: Implications For Iron Deficiency Chlorosis, En-Jung Hsieh, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) is an essential mineral that has low solubility in alkaline soils, where its deficiency results in chlorosis. Whether low Fe supply and alkaline pH stress are equivalent is unclear, as they have not been treated as separate variables in molecular physiological studies. Additionally, molecular responses to these stresses have not been studied in leaf and root tissues simultaneously. We tested how plants with the Strategy I Fe uptake system respond to Fe deficiency at mildly acidic and alkaline pH by measuring root ferric chelate reductase (FCR) activity and expression of selected Fe uptake genes and riboflavin synthesis genes. …

Corn Response To Nitrogen Management Under Fully-Irrigated Vs. Water-Stressed Conditions, Bijesh Maharjan, Carl J. Rosen, John A. Lamb, Rodney T. Venterea

Department of Agronomy and Horticulture: Faculty Publications

Characterizing corn (Zea mays L.) grain yield (GY) response to N is critical for maximizing profits, increasing N use efficiency and minimizing environmental impacts. Although a large database of GY response to N exists for highly productive soils, few data exist for less productive soils. While changes in precipitation are expected in the future, few studies have compared GY response to varying N management practices under conditions of varying water availability. We measured GY and basal stalk nitrate nitrogen (BSN) at harvest using split-applied urea at eight N rates under fully-irrigated (FI) and water-stressed (WS) conditions in a loamy …

Letter To The Editor Ws-2 Introgression In A Proportion Of Arabidopsis Thaliana Col-0 Stock Seed Produces Specific Phenotypes And Highlights The Importance Of Routine Genetic Verification, Mon-Ray Shao, Vikas Shedge, Hardik Kundariya, Fredric R. Lehle, Sally Ann Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Arabidopsis thaliana is an important model organism with a robust network of resources that has been of enormous value to the plant science research community. The use of isogenic material as a reference point or control is critical for many types of experiments in plant molecular biology and genetics. Recently, we noticed that some seed from a common source of the widely-used Columbia-0 (Col-0) strain gave rise to plants showing features atypical for this strain. Whole genome DNA-sequencing and allele-specific PCR assays confirmed that the abnormal individuals contain multiple introgressions from the ecotype Wassilewskija-2 (Ws-2), as described below. This emphasizes …

The Effects Of Manufactured Nanomaterial Transformations On Bioavailability, Toxicity And Transcriptomic Responses Of Caenorhabditis Elegans, Daniel L. Starnes

Theses and Dissertations--Plant and Soil Sciences

In recent decades, there has been a rapid expansion in the use of manufactured nanoparticles (MNPs). Experimental evidence and material flow models predict that MNPs enter wastewater treatment plants and partition to sewage sludge and majority of that sludge is land applied as biosolids. During wastewater treatment and after land application, MNPs undergo biogeochemical transformations (aging). The primary transformation process for silver MNPs (Ag-MNPs) is sulfidation, while zinc oxide MNPs (ZnO-MNPs) most likely undergo phosphatation and sulfidation. Our overall goal was to assess bioavailability and toxicogenomic impacts of both pristine, defined as-synthesized, and aged Ag- and ZnO-MNPs, as well as …

Growing South Dakota (Winter 2016), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue includes the 2015 South Dakota Agricultural Experiment Station Annual Report.

[Page] 2 Director's Message
[Page] 3 Concentrating On Cattle: Researcher Studying Mechanics To Improve Reproduction Efficiency In Beef Cattle
[Page] 5 At The Forefront: SDSU Diagnostic Lab Scientists Sequence Avian Influenza Genome
[Page] 6 Seeking Superior Wheat: Breeder Using Extensive Genome Testing To Improve Winter Wheat Cultivars
[Page] 8 Profiles In Leadership: Plant And Fungi Interactions May Offer New Plant Production Possibilities
[Page] 9 Targeting Emerging Diseases: Plant Pathologists Working To Better Manage Soybean & Sunflower Diseases
[Page] 10 Compelling New Crop: Carinata Offers Potential For Crop Diversity, …

Farmers’ Perception On The Health And Environmental Benefits Of Organic Rice Production In The Philippines: Implications For Further Policy Research, Guinevere T. Madlangbayan, Agnes C. Rola

Journal of Public Affairs and Development

Conventional rice production with the use of chemicals was found to have negative externalities both to the farmers’ health and the farming environment. Organic agriculture technologies were developed to minimize such impacts. This paper explores the empirical support of the health and environmental benefits of organic agriculture by generating data from a survey of rice farmers and focus group discussions in rice farming communities. The respondents came from rice farming areas in the country where early adoptors of the organic rice farming system were located. The results of the analysis suggest that farmers fail to recognize the relationship between the …

Modulation Of The Physiological And Biochemical Effects Of Copper Nanoparticles In Kidney Beans (Phaseouls Vulgaris) Treated By Kinetin, Suzanne Annette Apodaca

Open Access Theses & Dissertations

It is imperative to not only understand the impact of engineered nanomaterials (ENMs) in edible plants, but also the interactions they have with various additives used in agriculture. In this study, kidney bean plants were grown in potting soil treated with nano copper (nCu), bulk copper (bCu), and copper chloride (CuCl2) at concentrations of 0, 50, and 100 mg/kg for 55 and 90 days. At 15 days of growth, 0, 10, and 100 μm of kinetin (KN) were applied to plants. Plant tissue samples were harvested at 55 days and seeds were reaped at 90 days. Physiological and biochemical parameters …

Multi-Criteria Decision Analysis (Mcda) For Agricultural Sustainability Assessment, Byomkesh Talukder

Theses and Dissertations (Comprehensive)

Multi Attribute Utility Theory (MAUT), Preference Ranking Organization Method for Enrichment Evaluations (PROMETHEE) and Elimination methods of Multi-Criteria Decision analysis (MCDA) are tested to assess and compare the sustainability of different agricultural systems. Indicators and composite indicators are derived from data gathered using the agricultural sustainability categories of Productivity, Stability, Efficiency, Durability, Compatibility and Equity (PSEDCE).

Agricultural systems around the world face challenges from current agricultural practices, over-exploitation of natural resources, population growth and climate change. As a result, understanding agricultural sustainability has become a global issue. Assessment is a first step in benchmarking and tracking agricultural sustainability and …

Physical Properties Of Edible Composite Films Based On Chitosan And Whey Protein Isolate, Wang Yaosong, Zhao Liming, Wu Yanqing

Food and Machinery

The chitosan with different molecular weights (MW: 50 kDa, 250 kDa, 500 kDa and 1 000 kDa) were used to respectively combine whey proteins to form their composite films. It was expected that chitosan/whey proteins films endowed with versatile functionalities to meet practical requirement of food packaging applications. The equal volume of 3% (m/V) chitosan dissolved in 2% (m/V) acetic acid solutions and 12% (m/V) preheated (80 ℃ for 30 min) whey protein solutions was used to prepare resulting film-forming solutions after 2.4% (based on whey protein content, m/m) glycerol to make cast composite films. The functional propertied of the …

Analysis Of Amino Acids Composition And Nutritional Value Evaluation Of Dried Osmund, Li Xueying, Lu Ning, Zhang Jing, Xu Hui, Guo Bingzheng

Food and Machinery

To systematically analyze and evaluate amino acids of dried osmund, 17 kinds of amino acids were derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and then were analyzed by ultra performance liquid chromatography. L-tryptophan was quantitatively analyzed by ultraviolet spectrophotometric. Nutritional value evaluation of dried osmund protein was synthetically studied by methods of ratio coefficient of amino acid and fuzzy discernment. The results showed that the sample had 18 kinds of amino acids. The content of the total amino acids was up to 697.93 mg/g protein.The proportion of essential amino acids in total amino acids was 0.45, and the proportion of essential amino acids …

Determination On Salt Content Of Dry-Cured Meat By Rapid And Non-Invasion Impedance Technology, Chen Tianhao, Han Minyi, Wang Peng, Wei Ran, Xu Xinglian

Food and Machinery

The experiment was conducted to establish a new method to determine the salt content in cured meat and treated the meat with different salt content as samples. Impedance, salt content and water content were measured in the experiment and the results showed that the method would be a good choice for the determination of the salt content. Result: And, there was strong relationship between the impedance and the salt content. It is possible to construct some linear model between these two parameters. It indicated that there is good correlation between the predicted salt content and impedance magnitude (R²=0.980 …

Improvement In Methods For Activity Measurement Of Polyphenol Oxidase In Wheat, Zhou Xiaoling, Yang Daiming, Li Na

Food and Machinery

Three wheat varieties were selected as experimental materials to measure the activity of polyphenol oxidase According to the method from Measurement of Polyohenol Oxidase Activity in Wheat Flour in national standard draft. And found it has three deficiencies: first, because of the lack of crude extcation of polyphenol oxidase, it led to the less quantity of polyohenol oxidase, and couldn’t reflect the real state of the activity of polyphenol oxidase; second, because of the unsuitable reaction temperature, it couldn’t help the enzyme reaction completely; third, adding catechol before centrifuge made a part of product intercepted by precipitation, thus it has …

Effects Of Breaking Cell Wall On Rape Bee Pollen Treated With Pectinase Using Multi-Index Evaluation, Wang Kai, Ren Xiangnan, Dong Jie, Zhang Gensheng

Food and Machinery

To improve wall-broken effects of bee pollen, rape bee pollen was selected as material and treated with pectinase in different pH and temperature. The effects of pectinase on rape bee pollen were evaluated by protein dispersion index, soluble sugar content, microscopic analysis and particle size distribution. The results demonstrated that pectinase hydrolysis could disintegrate the intine at the germinal apertures, the optimal conditions were pH 3.5 and temperature 50 ℃. Under optimal conditions, protein dispersion index was 0.684 6, and soluble sugar content was 24.86%. By microscopic analysis, some germinal apertures seemed apart and some little particles emerged. According to …

Application Of Extrusion Technology For Preparing Defatted Black Soybean Meal Reconstituted Powder, Zhang Lin, Li Jianke, Ren Caixia, Sun Xuesi, Qin Lingxiang

Food and Machinery

The extruding and expanding experiment was studied with the black soybean meal as material, mixing corn starch by twin-screw extruder to make reconstituted powder, The best expanded technical parameters were determined, and studied the formula, the extrusion ratio, the reconstituability, the viscosity, the color of this powder flour. The optimal formula of the powder was as follows: black soybean meal 30%, corn starch 45%, sugar 15%, maltodextrin 10%, creamer 6%.

Analysis Of Amino Acid Composition And Nutritional Evaluation Of 22 Spirulina(Athrospira) Strains, Yan Chunyu, Wang Suying, Dong Shirui

Food and Machinery

No abstract provided.

Weight-Reducing Effect Of Onion Peel Extracts On Obese Rats, Liu Haoming, Pan Yannan, Cui Taihua, Wei Dan, Liang Chengyun, Li Guanhao

Food and Machinery

Investigated the effects of onion peel extracts on weight reduction in obesity rats. The experiment adopted the nutritional obesity model, and selected the tea polyphenol (TP) as a positive control and fed with high-fat diet added 0.57% of tea polyphenol and 1.33% of onion peel extracts. Research studied the influence of onion peel extracts on body weights, Lee’s indexes, food intake and food utilization rate of the rats, and further evaluated weight-reducing capacity of onion peel extracts according to above parameters. The results showed that onion peel extracts could decrease the body weight significantly by restraining fat cell dilated and …

Nutritional Benefits Of European Plums (Prunus Domestica L.), Liu Zhibin, Feng Jiangduo, Zhang Wen, Ni Li

Food and Machinery

European plum (Prunus domestica L.) and its dried products, commonly known as prune, are widely consumed in Unite States, European countries and worldwide. European plum, which is rich in polyphenols and other phytochemicals, possesses high nutritional values. This review summarizes the findings of published studies which examine the nutritional benefits, including antioxidant activity, the protection effect on cardiovascular system, anti colon cancer activity, relieving constipation effect and bone health improvement effect of European plum.

Advances In Research Of Cftr Inhibitors Extracted From Foods And Herbal Plants, Luan Jian, Yang Hong

Food and Machinery

CFTR chloride channel is the molecular drug target for the treatment of secretory diarrhea. Screening CFTR inhibitors is the most important and basic part of anti-diarrheal drug development. The natural products are diversified both in the structure and the biological activity. In the discovery of drugs and leading compounds, natural products play a key role and become the main source of drug development. Natural products from foods and herbal plants are usually safe for low or no toxicity and side-effect in clinic. CFTR inhibitors which source of foods and traditional Chinese medicines are important of study and treatment of CFTR …

Progress On Functionality Of Blueberry And Extracting Technology For Its Active Ingredient, Li Bin, Lei Yue, Meng Xianjun, Jiao Xinyao, Zhang Jiacheng, Wang Weisheng, Xu Qiang, Gao Ningxuan

Food and Machinery

For the its unique flavor, rich nutrients and multifunctionality, blueberry is highly popular. The nutritional value of blueberry is summarized, especially its healthy function in protecting eyesight, antioxidation and enhancing memory ability. Also, the current development situation of extractive technique of active ingredient in blueberry is reviewed and the prospect of blueberry is presented, aiming to provide a reference for further development of blueberry.

Research Advance On Preparation Of Peony Seed Oil By Aqueous Enzymatic Extraction, Chen Xuan, Zhou Bo, Wang Zhoujun, Zhong Haiyan

Food and Machinery

The peony seed oil is identified as new resources food in the laws, which can be applied to functional food, cosmetics, health care and other industries. The enzyme-assisted aqueous is a safe, efficient, and green method. Mainly discusses the basic composition of peony seed and its oil features, and analyzes the key factors of enzyme-assisted aqueous method for extraction of peony seed oil, which offers development direction in the crude enzyme extraction, enzymatic demulsification, immobilized enzyme technology.

Application Progress Of Gas Chromatography And Its Hyphenated Technology In Meat Products, Ye Yongli, He Xinrui, Chen Shien, Hu Xiaopeng, Ma Zhongren

Food and Machinery

As a mature technology, gas chromatography has been widely used in analysis of composition and content of material, and it is also promising for analysis of food nutrition components and quality and security as it is easy, fast to use. The application of gas chromatography and its hyphenated technology in nutrition ingredient analysis, flavor compound, freshness, pesticide and veterinary residue, and toxic chemical from environment and process of meat and meat products are reviewed, and then prospected its perspective applications in meat detection.

Rnai-Mediated Control Of Aflatoxins In Peanut: Method To Analyze Mycotoxin Production And Transgene Expression In The Peanut/Aspergillus Pathosystem, Renee S. Arias, Phat M. Dang, Victor S. Sobolev

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Food and Agriculture Organization of the United Nations estimates that 25% of the food crops in the world are contaminated with aflatoxins. That represents 100 million tons of food being destroyed or diverted to non-human consumption each year. Aflatoxins are powerful carcinogens normally accumulated by the fungi Aspergillus flavus and A. parasiticus in cereals, nuts, root crops and other agricultural products. Silencing of five aflatoxin-synthesis genes by RNA interference (RNAi) in peanut plants was used to control aflatoxin accumulation following inoculation with A. flavus. Previously, no method existed to analyze the effectiveness of RNAi in individual peanut transgenic events, …

Extension Agriculture And Natural Resources In The U.S. Midwest: A Review And Analysis Of Challenges And Future Opportunities, Mahdi M. Al-Kaisi, Roger Wesley Elmore, Gerald A. Miller, David Kwaw-Mensah

Department of Agronomy and Horticulture: Faculty Publications

This review addresses key changes in U.S. agricultural extension and future opportunities. Agricultural extension has been a part of the land-grant university (LGU) system for more than 100 years. The Morrill Act of 1862 established the LGU system by authorizing states access to federally controlled land and funding for public institutions offering educational opportunities focusing on agriculture and mechanical arts for farmers and the working class. Current surveys in Iowa reveal changing trends in extension: more than 90% of farmers identified private-sector crop advisers as their primary source for recommendations, whereas more than 80% of those advisors identified Iowa State …

A Regional Approach To Analysis Of Food Security Framework And Policies Of The Economic Community Of West African States (Ecowas), Hannah Elizabeth Shear

Graduate Theses and Dissertations

Food security is always a concern for consumers, but especially for those in West Africa, where the population of food insecure people has continued to grow over the past decade despite attempts by initiatives such as the World Food Program and the establishment of Millennium Development Goals. The people of West Africa are subject to repetitive natural disasters and are often in political upheaval. Many economic policies have been established to mitigate the impact that production disasters have on the consumers; one of these policies is the utilization of regional food reserves.

In 2010, the Economic Community of West African …

Modifying A Cow-Calf Biophysical Simulation Model For Analyses Of Alternative Enterprises, Jennifer L. Lutes

Graduate Theses and Dissertations

Cow-calf producers in the United States, tasked with providing beef calves for the beef industry, have had a multitude of difficulties to overcome in recent years. Producers in northwest Arkansas were negatively impacted by high hay prices coupled with low beef cattle market prices due to severe drought experienced in portions of 2010, 2011, and 2012. During this time they also faced high grain prices, due to a record low harvest, combined with portions of the corn harvest diverted from human and animal feed to ethanol production. Tight lending policies of this time, reminiscent of the housing market crash in …

Marketing Alaskan Seafood: Strategies With Brand Identification, Differentiation, And Promotions, Anna Jacobson

Journalism

Everyday the globe experiences changes with food. New methods in growing and harvesting are becoming approved, while others are frowned upon. People care more about where their food comes from than ever before. This research is to explore how Alaska seafood can successfully market their products in an ever-changing world. As challenges present themselves in the industry, opportunities are there as well.

This study sets out to explore marketing strategies for promoting a seafood brand. Through exploring current literature and interviewing experts to obtain data, conclusions will be drawn and analyzed. These findings will help to understand the best practices …

Producer Interest In A Food Hub: A Look At Attributes, Adaptations And Attitude, Jennifer Michele Muzinic

Graduate Theses/Dissertations

Demand for locally-sourced food in the United States has spurred the introduction of a number of food hubs in recent years. The objective of this study was to examine the attitudes of agricultural producers in the Missouri Ozarks towards participation in a food hub. A survey of 218 farmers was conducted to assess how likely producers were to sell into a food hub and whether those producers would be willing to alter their supply to accommodate the needs of the proposed hub. Descriptive statistics, factor analysis and regression analysis were used to uncover which variables were most likely to contribute …

Predicting Invasive Range Of Eucalyptus Globulus In California, Anthony J. Lopez

Master's Theses

Eucalyptus globulus is the subject of intense debate in California. Its invasive potential, aggressive growth, reproductive biology, and association with fire make the prediction of spread important in California. Two modeling simulations, Climex and Maxent, will illustrate the distribution and potential growth range of E. globulus in California based on climate and presence. Modeling the potential range of growth will predict invasive spread. The anticipated result is the continued spread of E. globulus in coastal regions with adequate moisture and suitable climate. Predicting the range of E. globulus in California dictates management strategy and is key for preventing further introduction, …