Digital Commons Network^™

Characterization Of The Inflammatory Phenotype Of Mycobacterium Avium Subspecies Paratuberculosis Using A Novel Cell Culture Passage Model, Jamie L. Everman, Torsten M. Eckstein, Jonathan Roussey, Paul M. Coussens, John Bannantine, Luiz E. Bermudez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Understanding the pathogenic mechanisms of Mycobacterium avium subspecies paratuberculosis (MAP) and the host responses to Johne’s disease is complicated by the multi-faceted disease progression, late-onset host reaction and the lack of available ex vivo infection models. We describe a novel cell culture passage model that mimics the course of infection in vivo. The developed model simulates the interaction of MAP with the intestinal epithelial cells, followed by infection of macrophages and return to the intestinal epithelium. MAP internalization triggers a minimal inflammatory response. After passage through a macrophage phase, bacterial reinfection of MDBK epithelial cells, representing the late phase of …

Effect Of Ditylenchus Dipsaci Kühn, 1857 (Tylenchida: Anguinidae) On Onionyield In Karaman Province, Turkey, Eli̇f Yavuzaslanoğlu, Abdullah Di̇ki̇ci̇, İbrahi̇m Hali̇l Elekci̇oğlu

Turkish Journal of Agriculture and Forestry

This study was conducted to investigate the effect of the stem and bulb nematode Ditylenchus dipsaci Kühn (Tylenchida: Anguinidae) on yield parameters of some onion varieties in Karaman Province, Turkey. Experiments were established in a naturally infested onion field during two consecutive years (2012-2013). Nematode population development was followed at the beginning and end of the experiment. The reproduction rate was also calculated. Onion yield and the representative average diameter and length of the onion cultivar bulbs were determined. Yield parameters of six onion varieties were compared on nematicide-treated and untreated control plots in the experiments. Nematode populations significantly decreased …

Auxin-Mediated Growth Of Rice In Cadmium-Contaminated Soil, Hussna Farooq, Hafiz Naeem Asghar, Muhammad Yahya Khan, Muhammad Saleem, Zahir Ahmad Zahir

Turkish Journal of Agriculture and Forestry

Pollution from heavy metals is a global problem that is very dangerous to the environment. Among the heavy metals, cadmium is receiving more and more attention because it is one of the most ecotoxic, making it very harmful for biological activity in soil, biodiversity, plant metabolism, and human and animal health. Phytohormones, produced by plants, control or regulate germination, growth, metabolism, or other physiological activities, and they can alleviate the toxic effects of heavy metals on plant growth. Auxin is the most abundant natural growth hormone, controlling apical dominance, tropism, elongation of shoots, and initiation of roots in plants. However, …

Increasing Student Interest In Poultry Science Careers Through 4-H, Peggy Lynn Armstrong

Walden Dissertations and Doctoral Studies

The demand for poultry science graduates to fill available positions in the poultry industry continues to increase. At the same time, there are not enough graduates to fill the positions. The purpose of this study was to investigate the implementation of the poultry curriculum in the 4-H program and student recruitment through the 4-H program into the Poultry Science Department. The targeted populations were 4-H leaders who implement the poultry curriculum and university students who were currently studying poultry. Career development theories suggest that previous experiences during adolescence can be a major influence in future vocation choice. A mixed methods …

Rural Year-Round Growing To Ameliorate A Possible Negative Effect From Climate Change, Kimberlie A. Brussa

Walden Dissertations and Doctoral Studies

The Intergovernmental Panel on Climate Change (IPCC) suggested that, as the world population grows, food and water shortages will become even more serious issues (IPPC's 2014 predictions about the future effects of climate change (CC), Year-round growing (YRG) may provide a way for communities to extend growing seasons, expand local farm systems, and provide food year round. This case study included a detailed analysis of responses from representatives of all sectors of rural Mesa County, Colorado, regarding YRG and a local food and farm plan due to CC. The case was bounded by time (6 months of data collection) which …

Fortifying Farms And Ranches Against Weed Invasion, Utah State University

All Current Publications

The traditional approach to weed management on most farms or ranches is similar to a person running on a treadmill. A treadmill allows a person to run or walk while staying in one place. As the exercise deck cycles around and around, the user exerts energy to maintain the set pace, but never really goes anywhere.

Agricultural producers spend a great deal of resources (time and money) on what can be thought of as a weed management treadmill. The weed treadmill is always in motion. Treadmill speed is controlled by how well or how poorly the weeds were managed in …

Tall Fescue Seed Extraction And Partial Purification Of Ergot Alkaloids, Huihua Ji, Franklin Fannin, J. Klotz, Lowell P. Bush

Kentucky Tobacco Research and Development Center Faculty Publications

Many substances in the tall fescue/endophyte association (Schedonorus arundinaceus/Epichloë coenophiala) have biological activity. Of these compounds only the ergot alkaloids are known to have significant mammalian toxicity and the predominant ergot alkaloids are ergovaline and ergovalinine. Because synthetically produced ergovaline is difficult to obtain, we developed a seed extraction and partial purification protocol for ergovaline/ergovalinine that provided a biologically active product. Tall fescue seed was ground and packed into several different sized columns for liquid extraction. Smaller particle size and increased extraction time increased efficiency of extraction. Our largest column was a 114 × 52 × …

Impacts Of Land-Cover Change And High Rainfall On Soil Erosion Among Three Farms In Cerro Punta, Chiriquí, Panamá., Madeline Happ

Independent Study Project (ISP) Collection

Soil erosion is a key source leading to the depletion of Earth’s natural resources. Beginning with deforestation, soils are stripped of their rich vegetation cover, leaving them vulnerable to wind, water, and sometimes total exhaustion. One of the major causes of deforestation, if not the primary cause, is for agricultural purposes. Extensive agricultural fields, steep slopes, and heavy rains characterize the highlands of Chiriquí; thus, the land displays potential for severe loss of soil. This paper illustrates and discusses the extent of soil erosion in Cerro Punta, Chiriquí, specifically considering the steep terrains and very wet environment that impact the …

Nông Thôn Mới And Rural Development In The Mekong Delta: A Comparative Study Of Bàu Môn And Hòa Phụng C Hamlets, Alex Lintz

Independent Study Project (ISP) Collection

Vietnam’s Nông Thôn Mới or New Rural Development program is a relatively new target program introduced in 2010. As of yet there has been little research done on its effects on the economies in rural areas. This paper attempts to analyze the economic situation in the Bàu Môn and Hòa Phụng C hamlets with a particular emphasis placed on income and factors that affect income in terms of Vietnam’s Nông Thôn Mới or New Rural Development program. In order to assess the economic situation in the hamlets, a survey was administered to 25 participants in each hamlet and leaders from …

Metabolic Engineering Of Oilseed Crops To Produce High Levels Of Novel Acetyl Glyceride Oils With Reduced Viscosity, Freezing Point And Calorific Value, Jinjie Liu, Adam Rice, Kathleen Mcglew, Vincent Shaw, Hyunwoo Park, Thomas E. Clemente, Mike Pollard, John Ohlrogge, Timothy P. Durrett

Department of Agronomy and Horticulture: Faculty Publications

Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2- diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina and soybean …

Agricultural Experiences And Factors Of Undergraduates Who Enroll In A College Of Agriculture, Benjamin G. Swan, Ann M. De Lay

Agricultural Education and Communication

Industry partners and College of Agriculture, Food and Environmental Sciences (CAFES) faculty have observed students entering the college possessed fewer agriculture experiences and skills than their predecessors. They have also lamented the increasing pressure to develop industry-ready students, when the gaps are ever wider between their experience and skills entering college and what are required upon graduation. During the 2011 spring quarter, all CAFES students (N = 3,366) were sent an electronic survey that resulted in 911 responses (27% response rate). Three quarters of the students were female and one third were seniors. Prior to enrolling at the university, 34% …

2013 Oregon Vineyard And Winery Census Report, Southern Oregon University Research Center

Oregon Wine Board Documents

This statewide survey report on vineyards and wineries in Oregon covers bearing and nonbearing acres, size of vineyard operation, variety and county, size distribution, prices, yields, crush, inventory, and sales. The report also contains some comparisons of data sor 2012 and 2013. According to this report, the wine industry continued to grow in multiple areas.

The View From Lu Cooperative Extension, December 2014, Lincoln University Cooperative Extension

The View from LU Cooperative Extension

Volume 2, Issue 1

Farming With An Amputation, Rhonda Miller Ph.D., Anne Reither

Archived Agriculture Publications

No abstract provided.

Root Stimulation Using Vermi-Products In Grape Vine Propagations, Katerina M. Flores

Wine and Viticulture

Vermi-products are byproducts produced from earthworms digesting and decomposing a natural material into another. Liquid teas or extracts of vermi-products are another form of leachate material found in the completed compost bin. These products are not high in essential plant macronutrients, have an average neutral pH (around 7.0), and are known for living microbial population. Grapevine propagations cut from the Trestle Vineyard at California Polytechnic State University, San Luis Obispo were used to observe root growth development using steer compost, vermicompost, and vermi-extract. Propagations used were dormant Pinot Noir clone 777. Compost subsamples tested 5%, 10%, 20%, and 40% by …

Effects Of Stocking Rate On Forage Nutrient Composition Of Nebraska Sandhills Upland Range When Grazed In Early Summer And The Effects Of Grazing On Nebraska Sandhills Meadow Forage Nutrient Composition, Jared Vern Judy

Department of Animal Science: Dissertations, Theses, and Student Research

The objectives of this research were to 1) evaluate the effects of stocking rate on forage nutrient quality 2) quantify the relative proportions of current vs. previous year growth being consumed in early summer upland range pastures and 3) determine how grazing effects forage nutrient quality in subirrigated meadows in the Nebraska Sandhills. Experiment 1 was a two year study conducted on the experimental upland range paddocks at Gudmundsen Sandhills Laboratory. Twelve 2-hectare paddocks were assigned one of three treatments stocked at 0 (control), 0.57 (light), and 0.85 (heavy) AUM/ha. Ten 0.25 m² quadrats were clipped per paddock during …

Proper Handling And Transportation Of Eggs For Sale At Kentucky Farmers’ Markets, Anthony J. Pescatore, Jacquie Jacob

Agriculture and Natural Resources Publications

Regardless of the number of eggs produced, and whether the eggs are for home use or sale, careful egg handling is very important. The United States Food and Drug Administration (FDA) lists eggs as a potentially hazardous food, therefore careful handling is important. In Kentucky, if you sell more than 60 dozen eggs a week you need to purchase a retail license. The same holds true if you want to sell your eggs to a retail store to be re-sold or to a distributor that will sell them for you. If you produce fewer than 60 dozen eggs a week …

Sheep Breeding: Heritability, Ebvs, Epds And The Nsip, Debra K. Aaron

Agriculture and Natural Resources Publications

Genetic improvement in a flock depends on the producer’s ability to select breeding sheep that are genetically superior for traits of economic importance. This is complicated by the fact that an animal’s own performance is not always a true indicator of its genetic potential as a parent.

Inbreeding In Sheep, Debra K. Aaron

Agriculture and Natural Resources Publications

Inbreeding is broadly defined as the mating of individuals that are related. Strictly speaking, however, all animals within a breed are related. So, in a sense, every purebred sheep producer practices some degree of inbreeding. In most cases this relationship is very slight. Therefore, inbreeding is more practically defined as the mating of individuals more closely related than the average of the breed. This practice includes mating brother to sister, sire to daughter and son to dam.

An Introduction To Sheep, Debra K. Aaron, Donald G. Ely

Agriculture and Natural Resources Publications

The information in this fact sheet was developed to provide a quick reference to the most frequently asked questions about sheep and sheep production.

Basic Sheep Genetics, Debra K. Aaron

Agriculture and Natural Resources Publications

Genetics is the science of heredity. It seeks to explain differences and similarities exhibited by related individuals. The application of genetics to livestock improvement is known as animal breeding. The objective of this fact sheet is to provide a refresher course on basic genetics and to show how knowledge of genetics can be used to improve sheep production.

Keeping And Using Flock Performance Records, Debra K. Aaron

Agriculture and Natural Resources Publications

Performance records serve as the cornerstone of any good livestock management program. Unfortunately, the task of collecting, maintaining and using performance records is the one area of livestock production in general that gets the least attention. This fact sheet provides ten reasons why all sheep producers need to keep performance records on their flocks. Then, some ways of maintaining and using those records are discussed.

Crossbreeding Considerations In Sheep, Debra K. Aaron

Agriculture and Natural Resources Publications

Crossbreeding is the mating of individuals from different breeds. To a certain extent, it is a simple concept, but embarking upon a crossbreeding program, in sheep or any other livestock species, involves long-term decisions.

The primary benefits of a crossbreeding program are heterosis and breed complementarity.

Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson

Master's Theses

Packed-bed digesters are an alternative to covered lagoon digesters for methane production and anaerobic treatment of dilute wastewaters such as dairy barn flush water. The physical media of packed-beds retain biofilms, often allowing increased treatment rates. Previous studies have evaluated several types of media for digestion of dilute wastewaters, but cost and media fouling have setback commercial development. A major operational cost has been effluent recirculation pumping.

In the present effort, a novel approach to anaerobic digestion of flush dairy water was developed at pilot-scale: broken walnut shells were used as a low-cost packed-bed medium and effluent recirculation was replaced …

Effect Of Different Extrusion Processing Parameters On Physical Properties Of Soy White Flakes And High Protein Distillers Dried Grains-Based Extruded Aquafeeds, Sushil Kumar Singh, Kasiviswanathan Muthukumarappan

Agricultural and Biosystems Engineering Publications

Nutritionally balanced ingredient blends for catla (Catla catla), belonging to the family Cyprinidae, were extruded using single screw extruder. The extrusion was carried out at five levels of soy white flakes content (21%, 29%, 40%, 52%, and 59% db), five levels of moisture content (15, 19, 25, 31, and 35% db) and five levels of barrel temperature (100, 110, 125, 140, and 150 ºC) using three different die nozzles (having L/D ratios 3.33, 5.83, and 7.25). Blends with net protein content of 32.5% contains soy white flakes, along with high protein distillers dried grains (HP-DDG), corn flour, corn gluten meal, …

So You Want To Produce Your Own Eggs?, Jacquie Jacob, Anthony J. Pescatore

Agriculture and Natural Resources Publications

Backyard chicken flocks are becoming popular throughout the country in urban, suburban and rural communities. Preparation is essential for a successful backyard flock.

Ecological, Physiological, And Molecular Characterization Of Annual Bluegrass (Poa Annua L.) Herbicide Resistance And Its Control On Golf Courses, Robert Cross

All Dissertations

Annual bluegrass (Poa annua L.) is one of the most pervasive, adaptable, and variable plant species in the world and is the most problematic winter annual weed in managed turfgrass. Its prolific seedhead production regardless of mowing height, clumping growth habit, and lack of tolerance to stress reduces turfgrass aesthetic quality and playability on golf courses. Its ability to germinate almost year-round influences cultural practices and herbicide use. Herbicides are an integral part of a weed control program for all weeds that invade a desirable turfgrass stand. However, inappropriate use of herbicides results in herbicide resistance issues. In recent years, …

Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt

Master's Theses

Packaging Materials account for 31% of the world’s municipal solid waste. Agencies like the Environmental Protection Agency (EPA) and the Agency for Toxic Substances and Disease Registry (ATSDR) are pushing for the increased use of recycled thermoplastic materials. Polyethylene terephthalate (PET) is a commonly recycled thermoplastic which is used to package ready-to-eat fruits and vegetables. Most recycled polyethylene terephthalate (RPET) packaging materials contain heavy metal catalysts, the most common being antimony. The recent increased use of recycled plastic materials has been suspected as the source of increased human heavy metal exposure. In this study, cadmium, chromium, nickel, lead and antimony …

The Economic Viability Of Cocoa Crop Insurance In Ghana, Justin D. Mckinley

Graduate Theses and Dissertations

This study was motivated by the fact that Ghanaian cocoa producers face lower yields than other main cocoa producing counties which in turn increases food insecurity for smallholder producers. In addition, low yields experienced by Ghanaian producers is a driving factor for forest degradation and deforestation as cocoa producers encroach further into previously undisturbed forests in efforts to increase their incomes. There are currently production methods to achieve higher yields readily available in Ghana; however, many producers choose not to adopt these methods because they are seen as too risky, or simply cannot adopt them due to financial/credit constraints. A …

European Consumers' Attitudes Towards Cisgenic Rice, Anne-Cécile Delwaide

Graduate Theses and Dissertations

The enhancement of existing plant breeding techniques, such as cisgenesis, allows plant breeders to enhance an existing cultivar quicker and with little to no genetic drag. Cisgenesis is the genetic modification of a recipient plant with natural gene(s) from a sexually compatible plant. Unlike transgenesis, which is the genetic modification of a recipient plant with gene(s) from any non‐plant organism, or from a donor plant that is sexually incompatible with the recipient plant, the results of cisgenesis could occur naturally over time. Currently, both cisgenic and transgenic products are classified as genetically modified organisms (GMOs) and are labeled as such …