Digital Commons Network^™

Investigation Of The State Of Dna Methylation In High And Low Protein Accumulating Maize Lines, Akash Lal

Student Theses and Dissertations

Transgenic plant-based production for industrial enzymes is an evolutionary tool. Green bio- factories have powerful potential to produce desirable product on the most economical and efficient level. Seed itself provides protein a very stable environment, solving the problem of storage. Transgenic plants easily scale up production. Increasing population, climate change and low amounts of arable land create a damaging situation for which a plant-based production system is beneficial. The transgenic plant-based production system rather than microbial fermentation is a solution for this rising scenario. Epigenetics is a natural phenomenon which causes heritable changes in gene expression alternative to the involvement …

Pando: Charismatic Megaflora And The Populus Paradox, Paul C. Rogers, Lance Oditt

Aspen Bibliography

Within the field of landscape ecology, the Pando aspen grove on the Fishlake National Forest of Utah is legendary—and it is in danger of dying from hotter, drier temperatures and other threats. Pando is one of the largest and oldest known still-living organisms on Earth. Quaking aspen are an important tree species in Greater Yellowstone and the Rocky Mountain West. Enjoy this essay written by a scientist who studies Pando and a photographer who celebrates this arboreal mega-being.

The Impact Of China’S Environmental And Trade Policies On U.S. Plastic And Paper Waste Exports, Savant Nzayiramya, John C. Beghin

Cornhusker Economics

This article documents major environmental and trade policy changes in China and their impact on plastic and paper waste trade between the U.S. and China and other trade partners. The article explains how U.S. plastic waste exports have been nearly annihilated by these policies. Paper waste trade is more resilient and has survived the policy shifts in China, although quite diminished.

Distribution Pattern And Phytosociological Study Of Trees Associated With Agroforestry In Poonch District Of Jammu And Kashmir, India, Javed Manzoor, Mohd Junaid Jazib

Journal of Bioresource Management

The focus of present study was to find the phytosociology, distribution pattern, and diversity of different species of trees associated with traditional agroforestry in the district of Poonch, Jammu and Kashmir, India. Data was collected through quadrat survey method. A total of fifty quadrats of the size (10x10m) each were thrown randomly in agriculture fields. Total 26 tree species belonging to 16 families and 21 genera were encountered in the agricultural fields with Grewia optiva, Pyrus persica and Celtis australis being the tree species in dominance. The most dense species of tree recorded was Grewia optiva with a density value …

Effects Of Intraguild Interactions On Anticarsia Gemmatalis And Chrysodeixis Includens Larval Fitness And Behavior In Soybean, Sabrina Ongaratto, Edson Ll Baldin, Thomas E. Hunt, Debora G. Montezano, Emily A. Robinson, Maria C. Dos Santos

Department of Agronomy and Horticulture: Faculty Publications

BACKGROUND: Crop pest management requires an understanding of the complex interactions among species that potentially impact crop yield. In soybean, the velvetbean caterpillar, Anticarsia gemmatalis (Hübner), and the soybean looper, Chrysodeixis includens (Walker), are described as key pests, sharing the same feeding guild. We assessed the intraguild interactions of these species under laboratory conditions. Fitness cost study was conducted to examine the influence of competition on insect development. A video tracking system was used to evaluate behavioral parameters during larval interactions in scenarios with and without food availability. RESULTS: In the fitness cost assay, pupal weight was not significantly affected, …

The Impact Of Covid-19 And Economic Policies On Nebraska Farm Real Estate In 2021, Jim Jansen, Jeffrey Stokes

Cornhusker Economics

Land industry professionals responding to the annual survey attributed the rise in Nebraska farm real estate values to current interest rate levels, crop prices, and COVID-19 disaster assistance payments provided to operators across the state. These forces substantially impacted farm and ranch finances across Nebraska. Operators faced an exceptional amount of risk and uncertainty when navigating production decisions during the height of the COVID-19 pandemic. Lower interest rates and disaster assistance payments provided stability to an industry facing an unpreceded economic shutdown and disruptions of supply chains.

Variations In Bacterial Community Structure And Antimicrobial Resistance Gene Abundance In Cattle Manure And Poultry Litter, Biyensa Gurmessa, Amanda J. Ashworth, Yichao Yang, Mary Savin, Philip A. Moore Jr, Steven C. Ricke, Giuseppe Corti, Ester Foppa Pedretti, Stefania Cocco

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle manure and poultry litter are widely used as fertilizers as they are excellent sources of nutrients; however, potential adverse environmental effects exist during land applications, due to the release of zoonotic bacteria and antimicrobial resistance (AMR) genes. This study was conducted to understand linkages between physiochemical composition, bacterial diversity, and AMR gene presence of cattle manure and poultry litter using quantitative polymerase chain reaction to enumerate four AMR genes (ermB, sulI, intlI, and blactx-m-32), Illumina sequencing of the 16 S region, and analysis of physical and chemical properties. Principal coordinate analysis of Bray–Curtis distance revealed distinct bacterial community structures …

Microbial Community Field Surveys Reveal Abundant Pseudomonas Population In Sorghum Rhizosphere Composed Of Many Closely Related Phylotypes, Dawn Chiniquy, Elle M. Barnes, Jinglie Zhou, Kyle Hartman, Xiaohui Li, Amy Sheflin, Ellen Marsh, Jessica Prenni, Adam M. Deutschbauer, Daniel P. Schachtman, Susannah G. Tringe

Department of Agronomy and Horticulture: Faculty Publications

While the root-associated microbiome is typically less diverse than the surrounding soil due to both plant selection and microbial competition for plant derived resources, it typically retains considerable complexity, harboring many hundreds of distinct bacterial species. Here, we report a time-dependent deviation from this trend in the rhizospheres of field grown sorghum. In this study, 16S rRNA amplicon sequencing was used to determine the impact of nitrogen fertilization on the development of the root-associated microbiomes of 10 sorghum genotypes grown in eastern Nebraska. We observed that early rhizosphere samples exhibit a significant reduction in overall diversity due to a high …

Arid Zone Rangeland Pastures And Fire, Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

Fire is integral to many ecosystems in the Western Australian rangelands. Rangeland fires affect more than just pastoralists and the businesses they run: these fires affect community safety and health, regional economies, societal and cultural values of landscape, biodiversity and tourism.

The Department of Primary Industries and Regional Development provides this information to support pastoral managers making decisions about sustainable resource use.

Spinifex Rangeland Pastures And Fire In Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

Fire is integral to many ecosystems in the Western Australian rangelands. Rangeland fires affect more than just pastoralists and the businesses they run: these fires affect community safety and health, regional economies, societal and cultural values of landscape, biodiversity and tourism.

The Department of Primary Industries and Regional Development provides this information to support pastoral managers making decisions about sustainable resource use.

A Microbiome Engineering Framework To Evaluate Rhizobial Symbionts Of Legumes, Kenjiro W. Quides, Hagop S. Atamian

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Background

For well over a century, rhizobia have been recognized as effective biofertilizer options for legume crops. This has led to the widespread use of rhizobial inoculants in agricultural systems, but a recurring issue has emerged: applied rhizobia struggle to provide growth benefits to legume crops. This has largely been attributed to the presence of soil rhizobia and has been termed the ‘rhizobial competition problem.’

Scope

Microbiome engineering has emerged as a methodology to circumvent the rhizobial competition problem by creating legume microbiomes that do not require exogenous rhizobia. However, we highlight an alternative implementation of microbiome engineering that focuses …

Soil Quality Indices As Affected By Long-Term Burning, Irrigation, Tillage, And Fertility Management, Helen C.S. Amorim, Amanda J. Ashworth, Kristofor R. Brye, Brian J. Wienhold, Mary C. Savin, Phillip R. Owens, Sergio H.G. Silva

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Understanding the impacts of long-term agricultural practices on soil quality (SQ) is key for sustaining agroecosystem productivity. This study investigated conventional and no-tillage (NT), residue burning and no burning, residue level (high and low), and irrigation (irrigated and dryland) effects on soil properties, SQ, and crop yields following 16 yr of a wheat (Triticum aestivum L.)–soybean [Glycine max (L.) Merr.] double-crop system via the Soil Management Assessment Framework (SMAF). A field experiment was conducted in the Lower Mississippi River Delta region on a silt-loam soil. Bulk density, soil organic C (SOC), total N (TN), pH, electrical conductivity (EC), and soil …

An Analysis Of Suicide Risk Factors Among Farmers In The Midwestern United States, Andrea Bjornestad, Courtney Cuthbertson, Jessie Hendricks

Counseling and Human Development Faculty Publications

Research on the complex relationships of variables contributing to farmer suicide is limited. The purpose of the study was to examine factors associated with suicide risk through the use of standardized instruments measuring psychological (depression, anxiety), social (social support), and contextual factors. A questionnaire was completed by 600 farmers in the Midwestern United States. A multiple linear regression model was used to analyze associations with suicide risk (SBQ-R), including depression (PHQ-9), anxiety (GAD-7), Brief COPE subscales (BC), social support (MSPSS), and select demographic and farming characteristics. The only variable that emerged as having a significant relationship with the natural log-transformed …

Managing And Buying Bulls, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Weaning percentage and therefore conception rate, can significantly influence profitability in the breeder herd.

Bulls need to be managed to optimise their performance for the mating period to maximise conception rates. For producers retaining females in the breeding herd, bulls can have a significant impact on future herd productivity through the genetics they pass on.

Various tools are available to assess how a bull will perform before purchase, including visual appraisal, obtaining a bull breeding soundness examination (BBSE) and utilising estimated breeding values (EBVs).

A Reporting Format For Leaf-Level Gas Exchange Data And Metadata, Kim S. Ely, Alistair Rogers, Deborah A. Agarwal, Elizabeth A. Ainsworth, Loren P. Albert, Ashehad Ali, Jeremiah Anderson, Michael J. Aspinwall, Chandra Bellasio, Carl Bernacchi, Steve Bonnage, Thomas N. Buckley, James Bunce, Angela C. Burnett, Florian A. Busch, Amanda Cavanagh, Lucas A. Cernusak, Robert Crystal-Ornelas, Joan Damerow, Kenneth J. Davidson, Martin G. De Kauwe, Michael C. Dietze, Tomas F. Domingues, Mirindi Eric Dusenge, David S. Ellsworth, John R. Evans, Paul P.G. Gauthier, Bruno O. Gimenez, Elizabeth P. Gordon, Christopher M. Gough, Aud H. Halbritter, David T. Hanson, Mary Heskel, J. Aaron Hogan, Jason R. Hupp, Kolby Jardine, Jens Kattge, Trevor Keenan, Johannes Kromdijk, Dushan P. Kumarathunge

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Leaf-level gas exchange data support the mechanistic understanding of plant fluxes of carbon and water. These fluxes inform our understanding of ecosystem function, are an important constraint on parameterization of terrestrial biosphere models, are necessary to understand the response of plants to global environmental change, and are integral to efforts to improve crop production. Collection of these data using gas analyzers can be both technically challenging and time consuming, and individual studies generally focus on a small range of species, restricted time periods, or limited geographic regions. The high value of these data is exemplified by the many publications that …

Nebline, March 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Naturally Occurring Elements in Groundwater - Part 1 of a Series — Calcium and Magnesium

Celebrate National Nutrition Month

RECIPE OF THE MONTH

2021 All-America Selections Vegetable Winners

Starting Seeds Indoors

Garden Guide Things to Do This Month

Planning for Spring Calving Season

Chemigation Trainings

2021 Nebraska On-Farm Research Annual Results Update Meetings

Houseplants: Simple Solutions to Plant Pests

Heart of 4-H Volunteer Award: Whitney Lehn

Meet 2020–2021 4-H Council

4-H Announcements for 4-H'ers and Volunteers

Private Pesticide Applicator Trainings by Zoom

EXTENSION CALENDAR

Upcoming Early Childhood Trainings

Effects Of Dietary Energy Levels On Performance And Carcass Yield Of 2 Meat-Type Broiler Lines Housed In Hot And Cool Ambient Temperatures, Pramir Maharjan, Katie M. Hilton, Garrett Mullenix, Jordan Weil, Antonio Beitia, Nawin Suesuttajit, Cole Umberson, Diego A. Martinez, Justina Caldas, Antonio Kalinowski, Nadia Yacoubi, Victor Naranjo, Judith A. England, Craig N. Coon

Poultry Science Faculty Publications and Presentations

Two meat-type broiler lines, line A and line B were fed experimental diets from 22-42 d with objectives to determine the effects of dietary metabolizable energy (ME) levels on feed intake (FI), performance, body composition, and processing yield as affected by environmental grow-out temperatures. Two thousand fifty male chicks from line A and 2,050 male chicks from line B were reared in 90-floor pens, 45 chicks per pen utilizing primary breeder nutrition and husbandry guidelines for starter (110 d) and grower (11-21 d) phases. Experimental finisher diets consisted of 5 increasing levels of apparent nitrogen corrected …

Research Note: Phytobiotics Modulate The Expression Profile Of Circulating Inflammasome And Cyto(Chemo)Kine In Whole Blood Of Broilers Exposed To Cyclic Heat Stress, Elizabeth S. Greene, Nima K. Emami, Sami Dridi

Poultry Science Faculty Publications and Presentations

Heat stress (HS) is a critical concern to the poultry industry as it affects both productivity and well-being. Various managerial and nutritional strategies have been proposed to mitigate the negative effects of HS in chickens, with plant-based additives showing promise. Recently, we reported the positive effect of a phytogenic feed additive (PFA) on growth performance in HS birds. Owing to the antioxidant nature of these compounds, we sought to further explore the effect of PFA on whole blood circulating chemokines, cytokines, and inflammasomes in HS broilers. Broilers (600 males, 1 d) were randomly assigned to 12 …

Development Of A Wild-Type Escherichia Coli Environmental Bloom Model To Evaluate Alternatives To Formaldehyde Fumigation In Broiler Chicken Hatch Cabinets, B. D. Graham, C. M. Selby, L. E. Graham, K. D. Teague, G. Tellez-Isaias, B. M. Hargis, C. N. Vuong

Poultry Science Faculty Publications and Presentations

Horizontal transmission of opportunistic Escherichia coli during hatch can have detrimental effects on early performance, particularly as pioneer colonizers. Commercially, formaldehyde is often applied in the United States to combat the bacterial bloom that occurs inside of the hatching environment. The purpose of these experiments was to develop a replicable E. coli horizontal challenge model to evaluate alternatives to formaldehyde sanitation applied to the hatching environment. In experiment 1, two trials were conducted for 2 wild-type (WT) E. coli isolates (isolate 1 [I1] or isolate 2 [I2]) to determine the appropriate in ovo challenge …

Mosaic Agriculture: A Guide To Irrigated Crop And Forage Production In Northern Wa, Geoff A. Moore Mr, Clinton K. Revell Dr, Christopher Schelfhout Dr, Christopher Ham Mr, Samuel Crouch Mr, Meredith M. Guthrie

Bulletins 4000 -

The Bulletin is a comprehensive guide for pastoralists, agronomists, agribusiness and the broader community on the growing of irrigated crops and pastures within a rangeland pastoral setting.

Dispersed irrigation developments on stations throughout the northern rangelands (sometimes referred to as mosaic agriculture) has created opportunities for the introduction of more productive forage species and pastoralists can now grow high quality forage for 12 months of the year. This can help to overcome the key constraint of traditional pastoral systems, the low quality of the feed over the dry season that typically results in stock losing condition.

Geographic Distribution: Eleutherodactylus Coqui (Common Coqui). Usa: Florida., Louis A. Somma

Papers in Herpetology

A geographic interception record for Eleutherodactylus coqui in Suwanee County, Florida.

Geographic Distribution: Iguana Iguana (Green Iguana): Usa: Florida., Louis A. Somma, Mark T. Bailey

Papers in Herpetology

A geographic distribution record for Iguana iguana in Marion County, Florida.

Cultivating Hops For Cone Production In Nebraska, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

The hop cone is the primary product of agronomic value when growing Humulus lupulus L. (common hop). Cones are modified stem and leaf structures that protect the female flower cluster that forms chemical compounds important for flavoring beer and other uses. The majority of hops in the United States (~96%) is grown in the states of Washington, Oregon, and Idaho. Hops can be grown in very diverse climates, but it is the climatic consistency of the Pacific NW that provides product consistency and reasoning commercial hops production is prevalent in the region. Hops is a niche crop outside of the …

Phytogenic Feed Additives Improve Broiler Feed Efficiency Via Modulation Of Intermediary Lipid And Protein Metabolism-Related Signaling Pathways, Joshua J. Flees, Bhaskar Ganguly, Sami Dridi

Poultry Science Faculty Publications and Presentations

Diets enriched with phytogenic feed additives (PFA) such as AV/HGP/16 premix (AVHGP), Superliv concentrate premix (SCP), and bacteriostatic herbal growth promotor (BHGP) with essential oils have been shown to improve feed efficiency (FE) in broilers. This FE improvement was achieved via modulation of hypothalamic neuropeptides, which results despite feed intake reduction, in increased breast yield without changes in body weight compared to the control group. To gain further insights into the mode of action of these PFA, the present study aimed to determine the potential involvement of signaling pathways associated …

Comparison Of Nutritional, Flavor And Functional Characteristics Of Tibetan Yak Butter At Different Altitudes, Xie Si-Wei, Liu Chun-Ai, Sun Shu-Guo, Luo Zhang, Yang Fei-Yang

Food and Machinery

The fatty acid composition, volatile components, rheological properties and antioxidant activity of yak butter at different altitudes (2 500~2 800 m, 3 500~4 000 m and above 4 300 m) in Tibetan were analyzed and compared. The results showed that altitude had significant effects on fatty acid composition, volatile components, rheological properties, and antioxidant activity (P<0.05). The unsaturated fatty acid content of yak butter at altitude of 2 500~2 800 m was the highest. Yak butter with an altitude of more than 4 300 m had pure smell and best flavor quality. Yak butter at altitudes of 3 500~4 000 m had the best fluidity. Yak butter at different altitudes had different antioxidant abilities, among which yak butter at altitudes ranging from 2 500 m to 2 800 m had the strongest DPPH free radical (66.99 mmol/100 g Trolox), hydroxyl free Trolox (26.71 mmol/100 g Trolox), superoxide anion scavenging ability (22.85%), and iron ion chelating antioxidant ability (1.41%).

Assessment Of The Edible Safety Of Licorice Oil, Dou Kang-Ning, Dong Bin

Food and Machinery

The toxicology of licorice oil was researched through the acute toxicity test of mice, genotoxicity test of mouse sperm deformity and subacute toxicity test, and made a safety evaluation on it. The experimental results showed that the acute oral LD₅₀ of licorice oil on mice was greater than 15.0 g/kg·BW, which was classified as non-toxic. The mouse sperm deformity genetic toxicity test was negative, indicating that the test substance had no mutagenic effect. No abnormal changes in animal health status, biochemistry, hematology indexes and organ tissue morphology were observed in the 30-day feeding experiment. Based on this, it was …

Fabrication Of Rice Protein Hydrolysate-Daidzein Composite Nanoparticles, Chen Xiu-Wen, Jiao Ye, Cui Bo, Zheng Xiang-Ming, Zou Fei-Xue, Cheng Yun-Hui

Food and Machinery

Rice hydrolysate (RPH) was obtained by using trypsin, and then the nanoparticles of RPH-daidzein composite were fabricated for daidzein delivering. The effects of hydrolysis degree (DH), RPH concentration and daidzein concentration on the characteristic of the composite nanoparticles were investigated by single factor experiments. When the RPH-daidzein composite nanoparticles was prepared under the conditions of a DH of 12%, an enzymatic hydrolysate concentration of 7.5 mg/mL and a daidzein concentration of 0.75 mg/mL, the particle size, polydispersity index and Zeta-potential of the nanoparticles were 449.71 nm, 0.23 and -22.30 mV, respectively. The encapsulation efficiency of daidzein was 60.07%, and the …

Ssdna-Templated Fluorescent Copper Nanoclusters For Label Free Detection Of Tetracyclines, Yang Li-Xia, Zeng Xi-Ke, Yi Zi

Food and Machinery

In this paper, a novel and label-free fluorescence sensing strategy has been developed for tetracyclines (TCs) detection in food sample by using poly(thymine) (poly T)-templated copper nanoclusters (CuNCs) as fluorescent probes. Under optimized conditions, the label-free fluorescent sensor achieved highly sensitive and selective detection of TCs with a detection limit of 0.01 μmol/L, and with a detection limit of 48 μg/kg in real samples. Furthermore, the method was successfully applied to the detection of TCs in milk and chicken samples. The relative standard deviation was less than 4.01%, and the recovery rate was between 97.67% and 110.23%. The strategy was …

Ultrasonic Enzymatic Extraction Of Total Flavonoids From Saussurea Involucrata Leaves And Its Antioxidant Capacity Analysis, Ji De-Rong, Liu Song-Qi, Xiong Kun-Yan, Li Meng, Hu Ming-Hu

Food and Machinery

Optimization of ultrasonic enzymatic extraction of total flavonoids from Saussurea involucrata leaves was studied by single factor and response surface experiments. The antioxidant capacity of the total flavonoids extracted from the leaves of S. involucrata was evaluated using the scavenging rate and total reducing power of DPPH·. The results showed that the best technology of total flavonoids from S. involucrata leaves by ultrasonic enzyme method was: adding the crushed leaves of S. involucrata to 30% ethanol solution, with the material liquid ratio 1∶60 (g/mL) and the ultrasonic treatment at 50 ℃, for 12 min. After adjusting the pH to 4.8, …

Study On Dna Barcoding Identification Technology Of Common Pathogenic Microorganisms In Food, Zhong Wen-Tao, Xu Yue, Wang Shu-Hao, Wang Fang-Mei

Food and Machinery

In order to explore the application prospects of DNA barcode technology in microbial identification, the 16S rRNA sequences of 8 common pathogenic microorganisms in food were compared. The PCR amplification, sequencing and database search were conducted followed by universal primers designing, which were purposed to establish a microbial identification method based on DNA barcode. The results showed that the variable region sequence of V3～V6 amplified provided sufficient resolution for Gammaproteobacteria Enterobacteriales, including Vibrionales, and Pseudomonadales to accurately distinguish pathogenic bacteria and related interfering bacteria, corresponding to Bacillibacteria (Bacillales) could be identified to the “genus” level.