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Growing South Dakota (Summer 2023), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

3 SDSU Little International Celebrates 100 years of Tradition
7 2023 CAFES Celebration of Faculty Excellence
11 South Dakota Agricultural Experiment Station Locations and Research Initiatives
15 Every Acre Counts
16 Undergraduate Research
19 Tanner Sloan: South Dakota State Wrestler & Animal Science Student Takes Home NCAA Division I & U23 World Silver Medals
21 2023 CAFES Outstanding Seniors
23 Robert Streeter: International Advocate for Wildlife Conservation
25 Collegiate Cattlemen's Club
27 Jim and Melody Mielke: Lifelong Donors Contribute to Agriculture in More Ways than One
29 Jackrabbits Now and Then

Leptospira Serovars Circulating Among Human, Cattle And Goats With Associated Risk Factors In Ngara And Kibondo Districts, North-Western Tanzania, Jestina V. Katandukila, Ackrey G. Sissa, Ginethon G. Mhaphi, Yeremia J. Chuhila, Fred D. Chibwana

Tanzania Journal of Science

Leptospirosis is a neglected zoonotic disease, prominent in north-western Tanzania where interactions among humans, livestock and wildlife is high. This study therefore, assessed Leptospira seroprevalence and associated risk factors by screening Leptospira serovars from blood sera of cattle, goats and humans in Ngara and Kibondo Districts using Microscopic Agglutination Test (MAT). Blood sera were positive for seven Leptospira serovars in which Sokoine, Sejroe and Grippotyhposa had higher seropositivity of ≥ 20.9%, whereas Leptospira serovars Kenya, Pomona, Lora, and Bataviae had seropositivity of < 10%. Variation of seropositivity and seroprevalence between Ngara and Kibondo Districts was insignificant (p = 0.0718) but varied significantly between humans, goats and cattle (p = 0.0183). Leptospira serovars Sejroe and Grippotyphosa were present in humans, goats and cattle. Sokoine, Pomona and Bataviae were co-positive in humans and goats, while serovars Kenya and Lora were co-positive in humans and cattle, indicating the possibility of dualistic transmission of Leptospira serovars in the ecosystem. Suggested risk factors associated with acquiring Leptospira bacteria were drinking contaminated water, feeding contaminated food and farming in contaminated soils. Prevalence of Leptospira in the current study alerts for health and economic risks in north-western Tanzania, which requires intensive education programs on leptospirosis transmission and avoidance.

Assessment Of Human Health Risk Of Zinc And Lead By Consuming Food Crops Supplied With Excessive Fertilizers, Kafeel Ahmad, Asma Ashfaq, Zafar Iqbal Khan, Shahzad Akhtar, Sana Rani, Farzana Siddique, Shamayem Aslam, Ghulam Mustafa, Rana Muhammad Zubair, Hafsa Memona, Aima Iram Batool, Muhammad Nadeem, Javed Shoukat, Shahzadi Mahpara, Mumtaz Akhtar, Muhammad Umer Farooq Awan, Ilker Ugulu, Hamid Raza, Muhammad Ameer Hamza

Journal of Bioresource Management

For the study of heavy metals impact on human beings, areas in Sargodha city that were supplied with various types of fertilizers were chosen. The three industrial areas; (Bhalwal, Sillanwali, and Sahiwal) of this city were explored for research reasons. The researchers wanted to know how much heavy metal was in the soil, food crops, and human. Excess fertilizer use contributes to global pollution. Farmyard manure, urea, and potassium chloride were used on Site 1; urea phosphate, manure, and ammonium sulphate were used on Site 2; and super phosphate, ammonium phosphate, and nitrate phosphate were used on Site 3. Samples …

Students’ Knowledge Of And Attitudes Toward Dairy Production: A Survey Methodology Report, Heather Akin, Babatope Akinyemi, Julia Mcquillan, Tami Brown-Brandl

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

This report presents findings from a pilot survey conducted among undergraduate and graduate students (N = 410) at the University of Nebraska-Lincoln about students’ perspectives on technology usage, consumption, and sustainability in dairy production systems. An interdisciplinary research team developed the survey instrument and report. The main purpose of this pilot study was to create and administer survey items to support further research on experiential education and outreach opportunities related to robotics in small-scale dairy production and rural economic development. Descriptive findings indicated that most students had some familiarity with dairy production and the nutritional aspects of dairy products …

A Complete Telomere-To-Telomere Assembly Of The Maize Genome, Jian Chen, Zijian Wang, Kaiwen Tan, Wei Huang, Junpeng Shi, Tong Li, Jiang Hu, Kai Wang, Chao Wang, Beibei Xin, Haiming Zhao, Weibin Song, Matthew B. Hufford, James C. Schnable, Weiwei Jin, China Agricultural University, Hainan Yazhou Bay Seed Laboratory

Department of Agronomy and Horticulture: Faculty Publications

A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of genomic research. Through generating deep coverage ultralong Oxford Nanopore Technology (ONT) and PacBio HiFi reads, we report here a complete genome assembly of maize with each chromosome entirely traversed in a single contig. The 2,178.6 Mb T2T Mo17 genome with a base accuracy of over 99.99% unveiled the structural features of all repetitive regions of the genome. There were several super-long simple-sequence-repeat arrays having consecutive thymine–adenine– guanine (TAG) tri-nucleotide repeats up to 235 kb. The assembly of the entire nucleolar organizer region of the 26.8 Mb array with …

Balsam Woolly Adelgid, Liz Rideout, Kate V. Richardson, Diane G. Alston, Ryan Davis, Darren Mcavoy, Lori R. Spears, Danielle Malesky, Liz Herbertson, Colleen Keyes

All Current Publications

This fact sheet describes balsam woolly adelgid, a tiny sucking insect that is a serious pest of true firs in forests, landscapes, and in seed and Christmas tree production. It includes a description of tree injury and symptoms, life history and identification, monitoring, management, and implications to forest health.

Extraction And Functional Characteristics And Structural Analysis Of Porcine Liver Salt Soluble Protein, Wang Hao-Yang, Liu Li-Li, Cheng Wei-Wei, Xu Bao-Cheng, Su Ke-Nan

Food and Machinery

Objective： Optimize the preparation process of salt-soluble porcine liver protein and improve the utilization rate of porcine liver by-products. Methods： Taking the extraction rate as the index, the effects of NaCl concentration, pH, extraction time and solid-liquid ratio on the extraction rate of SSLP were analyzed, and the optimal preparation process was determined by orthogonal rotation optimization test. Taking water soluble liver proteins （WSLP） as the control, the functional characteristics （solubility, foaming, foam stability, emulsification, emulsification stability, etc.） of SSLP were compared and analyzed, and Fourier transform infrared spectroscopy （FT-IR） was used, the structural changes were analyzed by low field …

Study On Metabolic Changes Of Crucian Carp Induced By Phoxim Based On 1H-Nmr Metabolomics, Li Hong, Li Dan, Li Li-Juan, Wu Ni

Food and Machinery

Objective: Standardize the use of phoxim to ensure the safety of aquatic products. Methods: Taking freshwater crucian carp as the research object, Selecting phoxim, an organic phosphorus pesticide often used in agriculture, as the representative of exogenous environmental pollutants. The changes in normal physiological metabolism of crucian carp exposed to phoxim were studied by using the ¹H-NMR metabonomics method combined with PCA, PLS-DA, OPLS-DA and other multivariate statistical methods. Based on this, the toxic effects of phoxim on crucian carp and the toxicological mechanism of phoxim in the crucian carp were deduced. Results: Compared with the control group, the …

Application Of Dna Barcoding Technology In The Identification Of Adulterated Ingredients Of Edible Blood Products, Li Jiong, Wu Qiong, Jiang Hai, Hu Ming-Jie, Xu Xin-Yi

Food and Machinery

Objective: A COI sequence-based full-length DNA barcoding technology was established to identify adulteration of seven animal-derived components （including pigs, cows, sheep, chickens, ducks, geese and rabbits） in edible blood products. Methods: After the blood products were extracted and purified by DNA extraction and PCR amplification, the clone sequencing results were submitted to the DNA barcode local database of the 7 blood products for sequence comparison. The pretreatment method, DNA extraction method and PCR amplification conditions of the blood products were also selected and optimized, and various common blood product adulteration models were established to study the lowest adulteration detection rate …

Changes Of Biogenic Amine Concentrations In Hard Cheese Made From Refrigerated Yak's Milk During Ripening, Song Xue-Mei, Qiu Ting, Liu Yu-Ying, Zyang Yan, Zhang Wei-Bing

Food and Machinery

Objective: The research aimed to reveal the changes of biogenic amines in cheese made from different refrigerated milk during ripening and evaluate its quality safety. Methods: The hard cheese made from yak milk refrigerated for 24, 48 and 72 hours was taken as the research object, and the biogenic amines in cheese during ripening were determined by high performance liquid chromatography. Results: During the ripening period from 0 to 6 months, the content of biogenic amine in hard cheese made from different refrigerated yak milk showed an increasing trend. When the cold storage time of yak milk was extended from …

Leaching Kinetics Analysis Of Total Flavonoids, Free Amino Acids And Quercetin From Penthorum Chinense Pursh Leaf Tea, Zhang Meng-Rui, Ran Xu, Zeng Li, Jia Li-Rong, Duan Fei-Xia

Food and Machinery

Objective: To explore the changes of the total flavonoids, free amino acids and quercetin extraction amount with the brewing time and temperature during the brewing process of Penthorum chinense Pursh （PCP） leaf tea and fit the extraction kinetics equation of the three and calculate the kinetics parameters. Methods: The total flavonoids, free amino acids and quercetin were determined by ultraviolet spectrophotometry and high performance liquid chromatography （HPLC）. The second-order kinetic model and Arrhenius formula were used to fit the kinetics and calculate the kinetic parameters. Results: The leaching amount of total flavonoids and quercetin increased with the increase of the …

Effect Of Dry-Aging Processes On The Quality Of Rump Beef From Yanbian Cattle, Sun De-Peng, Peng An-Qi, Tan Jin-Long, Piao Chun-Xiang, Lu Ai-Hui, Mu Bai-De, Li Guan-Hao

Food and Machinery

Objective: This study aimed to improve the tenderness and flavor content of Yanbian cattle beef. Methods: Rump beef of Yanbian cattle were dry-aged by using three different methods, i.e., Method A: RH（65±5）% aging for 10 days, RH（75±5）% aging for 10 days, and then RH （85±5）% aging for 20 days; Method B: RH（75±5）% aging for 20 days and then RH（85±5）% aging for 20 days; Method C: RH（85±5）% aging for 40 days. The quality changes caused by the different aging processes were then analyzed and compared. Results: The cooking loss of the beef processed by all the three methods increased significantly with …

Ultrasound-Assisted Enzymatic Extraction Of Polysaccharides From Corn Bran And Determination Of Their Antioxidant Activity, Ma Li-Yuan, Huang Xue-Ying, Shang Er-Kun, Guo Li, Zhang Ya-Na

Food and Machinery

Obiective: In the study, corn bran powder was used as raw material, and the cell wall was destroyed by ultrasonic vibration and enzymatic hydrolysis, so that corn bran polysaccharide could be dissolved more fully. Methods: The extracted amount of polysaccharide was taken as the index, response surface test optimization was carried out on the basis of single factor test. And the antioxidant activity of corn bran polysaccharide was determined. Results: The optimal extraction process of corn bran polysaccharide was obtained as follows: mass ratio of cellulase to pectinase 2∶1, enzyme dosage was 2% of the quality of corn bran powder, …

Research Progress In Detection Technologies Of Mycotoxins In Cereals And Cereal-Based Products, Niu Can-Jie, Ye Su-Dan, Hu Yu-Xia, Zhou Xiao-Hong

Food and Machinery

Cereals and cereal-based products are easily polluted by mycotoxins in all aspects of production, storage and transportation, and mycotoxins are difficult to prevent due to their variety, low concentration, strong toxicity andlarge difference in nature. This paper summarizes the new development of the pretreatment technologies （liquid-liquid extraction, solid-phase extraction, QuEChERS, immunoaffinity chromatography） and detection technologies （immunochromatography technology, optical spectrum technology, liquid chromatography and liquid chromatography-mass spectrometry） of mycotoxins in cereals and products. The development trend of mycotoxin detection technologies was also prospected.

Research Progress On The Digestion, Absorption And Function Of Milk Fat, Zhu Qin-Xin, Chen Yue, Zhong Jin-Jing, Hou Yan-Mei, Zhong Hai-Yan, Zhao Yi-Qing

Food and Machinery

Milk fat globule （MFG） is secreted by mammalian glands and composed of a triacylglycerol core enclosed by a complex triple membrane structure, referred as the milk fat globule membrane （MFGM）. MFGM contains complex lipids and proteins, with nutritional, immunological, neurological and digestive functions. However, the relationship between functions and gastrointestinal digestion and the factors that influence the gastrointestinal digestion of milk fat have not been fully studied. In this paper, the structure composition, functional characteristics, gastrointestinal digestion and the factors affecting the digestion and absorption of milk fat were reviewed in order to provide an insight for the research on …

Research Progress On Extraction, Biological Activities And Product Development Of Polysaccharides From Cicada Flower, Jia Guo-Jun, Wang Xiao-Ying, Yun Jian-Min, Dong Jian-Fei

Food and Machinery

Cicada flower is a valuable edible and medicinal mushroom. Polysaccharides are the most important active ingredients of cicada flower. This paper reviewed the extraction, separation and purification technologies of cicada flower polysaccharides in recent years. Also, it analyzed the biological activities of cicada flower polysaccharides, such as antioxidant, anti-tumor, immune regulation, bacteriostasis, hypolipidemic, hypoglycemic, anti-virus and ease pain. Moreover, the development and application of products were summarized and the existing problems and future research directions were also discussed.

Pollution Status And Research Progress On The Detection Technology Of Alternaria Mycotoxins In Foods, Wang Xia, Feng Dong-Sheng, Tong Jin-Rong, Gao Meng-Feng, Zhang Wei-Yi

Food and Machinery

Alternaria mycotoxins are the secondary metabolites of Alternaria species, which have obvious toxicity （mutagenicity, teratogenicity and carcinogenicity, etc） to humans and animals. Alternariol （AOH）, alternariol monomethyl ether （AME）, alternuene （ALT）, tenuazonic acid （TeA） and tenoxin （TEN） are the most studied at present. Alternaria mycotoxins was reported in apples, orange, tomatoes, corns and other agricultural products. Eating these foods contaminated by Alternaria mycotoxins will cause serious harm to human health. In this paper, the pollution status and the detection technology of Alternaria mycotoxins were summarized, and the future research direction of Alternaria mycotoxins was prospected. The purpose was to provide …

Research Progress On Immune Function Of Food-Derived Bioactive Peptides, Zhang Zhi-Meng, Ni Ce, Ou Xiao-Hui, Cao Tian-Hong, Cheng Yun-Hui, Wen Li

Food and Machinery

Food-derived bioactive peptides play an immunoregulation role by regulating signal pathways and influencing the expression of cytokines in vivo. This review summarizes immunoregulatory effects of the food-derived protein hydrolysates and bioactive peptides on cells, animal models and human beings, and the immune mechanism of food-derived bioactive peptides in different models is explained. Finally, the future application of bioactive peptides is prospected.

Review Of Toxicity Of Agricultural Chemicals And Implications For Aquatic Fauna Of The Keep River, Samanatha Reynolds, David Morgan

Natural resources commissioned reports

This report was compiled for the Western Australian Department of Primary Industries and Regional Development (DPIRD) as part of the National Water Grid Authority (NWGA) project entitled “Managing water quality to enable future irrigation development in the Kimberley Region, WA”. The purpose of this report is to review the toxicity of farm chemicals (herbicides and insecticides) used by farm operators in the Keep River catchment and their potential impacts on the aquatic species in the river, in particular those species that are listed under the Environment Protection and Biodiversity Conservation Act 1999. It is designed to complement the report entitled …

Development Of A Matrix Reference Material For Amoxicillin In Lyophilized Bovine Milk, Liu Tian-He, Wei Ning, Wu Cai-Hong, Zhang Yu, Liu Qiu-Hui

Food and Machinery

Objective： This study aimed to provide the basis of quantity traceability for the analysis of amoxicillin residues in milk. At the same time, it can also provide quantity standards for ensuring the effective implementation of amoxicillin monitoring in milk and combating the abuse of amoxicillin in the country and regions. Methods： Two healthy middle-class dairy cows were injected with amoxicillin intramuscularly. After the natural metabolism of the drug for a period of time, the milk containing the target drug amoxicillin was collected and made into milk matrix reference material containing amoxicillin by vacuum freeze-drying technology. The uniformity test, stability test, …

Application Of Immunoassay In The Detection Of Amantadine Residues, Wang Lin, Wang Yao, Hu Xiao-Fei, Wang Cheng-Bin, Wu Jia-Bei

Food and Machinery

This review summarized and analyzed the immunoassay techniques, such as enzyme-linked immunosorbent assay, immunochromatographic assay, and chemiluminescence immunoassay, which have been applied in the detection of amantadine residue, and provides an outlook on their development.

A Policy Proposal For Agricultural Data Governance, Ana Sofía Castellanos Santamaría

Dissertations and Theses

As the digital economy continues to grow and data becomes increasingly important, effective data governance is essential. A data governance framework enables the efficient management, sharing, and integration of data, resulting in better decision-making, increased productivity, and enhanced innovation across industries, including agriculture. However, the agricultural sector in the United States is lagging behind other industries in the adoption of effective data governance practices. Agricultural data governance presents a unique set of challenges due to the wide range of stakeholders involved and the ever-increasing volume of data generated by digital technologies in farming. One key challenge to achieving the benefits …

Data On Plant Defense Enzyme Activity Associated With Three Endophytes Against Cornus Florida Erysiphe Pulchra Powdery Mildew, Emily Rotich, Margaret T. Mmbaga

Agricultural and Environmental Sciences Faculty Research

Three bacteria endophytes that colonize flowering dogwood (Cornus florida) suppressed Erysiphe pulchra powdery mildew disease severity. The three bacteria identified as Stenotrophomonas sp. (B17A), Serratia marcescens (B17B), and Bacillus thuringiensis (IMC8) were assessed for plant defense enzymes associated with plant protection. Detached leaves inoculated with powdery mildew were spray treated with the selected bacterial isolates and incubated for 15 h, 26 h, 48 h and 72 h and then analyzed for activation of defense enzymes and Pathogenesis related (PR) proteins associated with induced systemic resistance (ISR) as a potential mode of action against powdery mildew. At each time …

Reducing Scope 3 Emissions By Investing In Regenerative Agriculture In Supply Chains, Stephanie Cain

Master's Theses

The agricultural industry has an opportunity to shift to a more sustainable practice that helps restore vital topsoil, improve water quality, reduce environmental impact, and sequester atmospheric carbon into the vast soil carbon pool. However, to implement these practices at considerable scale, agricultural producers require access to resources and capital they rarely have and can be difficult to acquire. As a company, investing in regenerative agriculture in supply chains can lead to reduced Scope 3 emissions, more resilient supply chains, and better marketability as an investment fund, an employer, and a brand. Insetting regenerative agriculture can protect supply chains against …

Exogenous Application Of Growth Regulators Improves The Growth And Quality Of Dahlia Variabilis L., Shahbaz Khan, Naeem Abbas, Zuhair Hasnain, Sami Ullah, Imran Hussain, Afroz Rais, Muhammad Atif Ghafoor, Danish Ibrar, Sohail Irshad, Maham Saddique, Muhammad Amjad Bashir, Samra Naseem, Muhammad Musawir Khan, Jawaher Alkahtani

The Philippine Agricultural Scientist

Dahlia (Dahlia variabilis L.), a bulb crop sold as cut flower, garden ornamental/potted plant, becomes a popular cut flower in the world. This experiment was designed to study the effects of foliar applied gibberellic acid, zinc, ascorbic acid, and thiamine on the growth and flower attributes of dahlia. The study was conducted under greenhouse conditions at the Floriculture Research Area, University of Agriculture, Faisalabad, Pakistan. There were thirteen treatment with three levels each; gibberellic acid, zinc sulphate, ascorbic acid, and thiamine. First, foliar application was done at vegetative stage (after 45 d of transplantation) and second, at the flowering …

Nebline, June/July 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Introducing Nit Kits: Extension's New Program on Head Lice in Early Childhood Settings by Kait Chapman

Curiosity as a Tool for Health Access by Emily Gratopp

Recipe of the Month: Refreshing Watermelon Salad by Emily Gratopp

Nebraska Celebrates Wildflower Week, June 5–11 by Sarah Browning

Graden Guide: Things to Do This Month

Fescue Toxicosis by Connor Biehler

Graywater Use in Nebraska by Becky Schuerman

Aphids, Scales and Spider Mites, Oh My! by Kait Chapman

Challenging Behaviors: Using Positive Language by Hayley Jackson

Extension News: New 4-H Assistant

4-H’ers Qualify for State Presentations Contest

4-H Clubs Helped at Kiwanis Karnival

Nebraska …

Impact Of Nitrogen Availability On The Accumulation Of Vegetative Lipids In Sorghum, Leticia Felicio Pasqualino

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum (Sorghum bicolor (L.) Moench) is a versatile and prosperous feedstock crop for renewable energy production. With the increase in bioenergy demand, the production of higher oil content per biomass in a feedstock crop is a highly desirable trait for the biofuel conversion process yield. Especially if this outcome can be achieved using fewer inputs in the field, such as nitrogen. In microalgae species, nitrogen limitation has been associated with changes in the carbon storage metabolic pathway favoring triacylglycerol (TAG) accumulation. In this context, this study aimed to assess whether nitrogen starvation would result in a similar outcome in …

Desert Locust (Schistocerca Gregaria) Invasion Risk And Vegetation Damage In A Key Upsurge Area, Raphael Mongare, Elfatih M. Abdel-Rahman, Bester Tawona Mudereri, Emily Kimathi, Simon Onywere, Henri E.Z. Tonnang

All Peer-Reviewed Publications

In the recent past, the Horn of Africa witnessed an upsurge in the desert locust (Schistocerca gregaria) invasion. This has raised major concerns over the massive food insecurity, socioeconomic impacts, and livelihood losses caused by these recurring invasions. This study determined the potential vegetation damage due to desert locusts (DLs) and predicted the suitable habitat at high risk of invasion by the DLs using current and future climate change scenarios in Kenya. The normalized difference vegetation index (NDVI) for the period 2018–2020 was computed using multi-date Sentinel-2 imagery in the Google Earth Engine platform. This was performed to assess the …

Intensification Differentially Affects The Delivery Of Multiple Ecosystem Services In Subtropical And Temperate Grasslands, Shishir Paudel, Nuria Gomez-Casanovas, Elizabeth H. Boughton, Samuel D. Chamberlain, Pradeep Wagle, Brekke L. Peterson, Rajen Bajgain, Patrick J. Starks, Jefferey Basara, Carl J. Bernacchi, Evan H. Delucia, Laura E. Goodman, Prasanna H. Gowda, Ryan Reuter, Jed P. Sparks, Hilary M. Swain, Xiangming Xiao, Jean L. Steiner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Intensification, the process of intensifying land management to enhance agricultural goods, results in “intensive” pastures that are planted with productive grasses and fertilized. These intensive pastures provide essential ecosystem services, including forage production for livestock. Understanding the synergies and tradeoffs of pasture intensification on the delivery of services across climatic regions is crucial to shape policies and incentives for better management of natural resources. Here, we investigated how grassland intensification affects key components of provisioning (forage productivity and quality), supporting (plant diversity) and regulating services (CO2 and CH4 fluxes) by comparing these services between intensive versus extensive pastures in subtropical …

Integrative Analyses Of Genomic And Metabolomic Data Reveal Molecular Mechanisms Associated With Uterine Disease Traits In Transitioning Dairy Cattle, Leanna C. Sanchez, Mohammed Abo-Ismail, Daniel Peterson, Fernando Campos-Chillon

Master's Theses

The metritis complex (MC), characterizing post-partum uterine diseases in dairy cattle has negative implications on animal welfare, production efficiency, and the economic stability of the dairy industry. The studies in this thesis aimed to investigate the genetic architecture of the metritis complex and identify genomic regions and metabolites associated with the development of MC. Thereby enhancing our understanding of the biological pathways and molecular mechanisms involved in the pathophysiology of MC during the transition period in Jersey and Holstein dairy cattle.

Chapter 2 sheds light on the previous work done on MC. The goals of this review were to (1) …