Digital Commons Network^™

Joint International Grassland And International Rangeland Congress Kenya 2021: Grasslands Summary, James L. Klotz, J. H. Bouton

IGC Proceedings (1977-2023)

This summary used as its main sources the plenary and keynote papers and talks in Sub-theme 2: Forage Production and Utilization and Sub-theme 3: Livestock Production Systems as well as a sampling of oral talks presented at the XXIV International Grassland Congress (IGC). This IGC was held virtually and jointly with the International Rangeland Congress and hosted in Nairobi, Kenya during 23-29 October 2021. The XXIV IGC was also the first to be held on the African continent in Congress' 94-year history. The summary is further focused on that information presented under the concept "cultivated grasslands" (e.g., a small number …

Physicochemical Analysis Of Cold Brew And Hot Brew Peaberry Coffee, Evan T. Schwarzmann, Marlena P. Washington, Niny Z. Rao

College of Life Sciences Faculty Papers

Peaberry coffee is the result of a natural mutation of coffee beans, and they make up only about 5–7% of coffee crops. A typical coffee cherry contains two seeds that are developed against each other, resulting in the distinctive half-rounded shape of coffee beans. However, failing to fertilize both ovules of one of the seeds or failure in endosperm development can cause only one of the seeds to develop, resulting in smaller, denser beans with a more domed shape. Peaberry coffees are said to be sweeter, lighter, and more flavorful since the peaberry beans receive all nutrients from the coffee …

2023 Western Australian Crop Sowing Guide, B. Shackley, B. Paynter, J. Bucat, M. Seymour, H. Dhammu

Bulletins 4000 -

Welcome to the 2023 edition of the Crop Sowing Guide for WA, which introduces 31 new variety releases: two wheat, seven barley (one as feed only and six currently under malt evaluation), 15 canola, three hay-only oat and four lentil varieties.

The Crop Sowing Guide for WA has been compiled by officers in the Department of Primary Industries and Regional Development. It provides information to support variety decisions for each of the major crops for the upcoming season.

In this edition, the canola NVT series are now identified by GRDC/NVT as ‘Low-Med Rainfall’ and ‘Med–High Rainfall’ to reflect the environments …

Pestfacts Wa Issue 18 - October 2022, Cindy L. Webster, Svetlana Micic, Alan Lord, Ciara Beard, Andrea Hills, Daniel Huberli

PestFacts WA

PestFacts WA Issue 18 - October 2022 contents:

• Avoid snail contamination in wheat
• Be mindful of chemical withholding periods if spraying for native budworm
• Glume blotch and false (pseudo) black chaff in wheat
• Powdery mildew found in canola and lupin
• Rhizoctonia bare patches are common this year

Cyclic Adp Ribose Isomers: Production, Chemical Structures, And Immune Signaling, Mohammad K. Manik, Yun Shi, Sulin Li, Mark A. Zaydman, Neha Damaraju, Samuel Eastman, Thomas G. Smith, Weixi Gu, Veronika Masic, Tamim Mosaiab, James S. Weagley, Steven J. Hancock, Eduardo Vasquez, Lauren Hartley-Tassell, Nestoras Kargios, Natsumi Maruta, Bryan Y.J. Lim, Hayden Burdett, Michael J. Landsberg, Mark A. Schembri, Ivan Prokes, Lijiang Song, Murray Grant, Aaron Diantonio, Jeffrey D. Nanson, Ming Guo, Jeffrey Milbrandt, Thomas Ve, Bostjan Kobe

Department of Agronomy and Horticulture: Faculty Publications

Cyclic adenosine diphosphate (ADP)–ribose (cADPR) isomers are signaling molecules produced by bacterial and plant Toll/interleukin-1 receptor (TIR) domains via nicotinamide adenine dinucleotide (oxidized form) (NAD+) hydrolysis. We show that v-cADPR (2′cADPR) and v2-cADPR (3′cADPR) isomers are cyclized by O-glycosidic bond formation between the ribose moieties in ADPR. Structures of 2′cADPR-producing TIR domains reveal conformational changes that lead to an active assembly that resembles those of Toll-like receptor adaptor TIR domains. Mutagenesis reveals a conserved tryptophan that is essential for cyclization. We show that 3′cADPR is an activator of ThsA effector proteins from the bacterial antiphage defense system termed Thoeris and …

Spatial Patterns Of Woody Plant Encroachment In A Temperate Grassland, Dillon T. Fogarty, Robert B. Peterson, Dirac Twidwell

Department of Agronomy and Horticulture: Faculty Publications

Context Woody encroachment is the process whereby grasslands transition to a woody-dominated state. This process is a global driver of grassland decline and is ultimately the outcome of increased woody plant recruitment in grasslands. Yet, little is known about how recruitment distances structure spatial patterns of encroachment.

Objectives Here, we develop a recruitment curve to describe the scatter of woody plant recruitment around seed sources and examine how this structures spatial patterns of encroachment.

Methods We developed a recruitment curve for Juniperus virginiana using an encroachment dataset that captures spread from tree plantings into treeless grassland sites in the Nebraska …

The Heterogeneity In The Landscape Of Gene Dominance In Maize Is Accompanied By Unique Chromatin Environments, Liangwei Yin, Gen Xu, Jinliang Yang, Meixia Zhao

Department of Agronomy and Horticulture: Faculty Publications

Subgenome dominance after whole-genome duplication (WGD) has been observed in many plant species. However, the degree to which the chromatin environment affects this bias has not been explored. Here, we compared the dominant subgenome (maize1) and the recessive subgenome (maize2) with respect to patterns of sequence substitutions, genes expression, transposable element accumulation, small interfering RNAs, DNA methylation, histone modifications, and accessible chromatin regions (ACRs). Our data show that the degree of bias between subgenomes for all the measured variables does not vary significantly when both of the WGD genes are located in pericentromeric regions. Our data further indicate that the …

Next-Generation Technologies Unlock New Possibilities To Track Rangeland Productivity And Quantify Multi-Scale Conservation Outcomes, Caleb P. Roberts, David E. Naugle, Brady W. Allred, Victoria M. Donovan, Dillon T. Fogarty, Matthew O. Jones, Jeremy D. Maestas, Andrew C. Olsen, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

Historically, relying on plot-level inventories impeded our ability to quantify large-scale change in plant biomass, a key indicator of conservation practice outcomes in rangeland systems. Recent technological advances enable assessment at scales appropriate to inform management by providing spatially comprehensive estimates of productivity that are partitioned by plant functional group across all contiguous US rangelands. We partnered with the Sage Grouse and Lesser Prairie-Chicken Initiatives and the Nebraska Natural Legacy Project to demonstrate the ability of these new datasets to quantify multi-scale changes and heterogeneity in plant biomass following mechanical tree removal, prescribed fire, and prescribed grazing. In Oregon’s sagebrush …

Cytogenetic And Genomic Characterization Of A Novel Tall Wheatgrass‑Derived Fhb7 Allele Integrated Into Wheat B Genome, Wei Zhang, Tatiana Danilova, Mingyi Zhang, Shuangfeng Ren, Xianwen Zhu, Qijun Zhang, Shaobin Zhong, Linda Dykes, Jason Fiedler, Steven Xu, Katherine Frels, Stephen Wegulo, Jeffrey Boehm Jr., Xiwen Cai

Department of Agronomy and Horticulture: Faculty Publications

A novel tall wheatgrass-derived (Thinopyrum elongatum, genome EE) Fhb7 allele, designated Fhb7^The2, was identified and integrated into the wheat B genome through a small 7B–7E translocation (7BS·7BL–7EL) involving the terminal regions of the long arms. Fhb7^The2 conditions significant Type II resistance to Fusarium head blight (FHB) in wheat. Integration of Fhb7^The2 into the wheat B genome makes this wild species-derived FHB resistance gene usable for breeding in both common and durum wheat. By contrast, other Fhb7 introgression lines involving wheat chromosome 7D can be utilized only in common wheat breeding programs, not in durum …

How Digital Is Agriculture In A Subset Of Countries From South America? Adoption And Limitations, Laila A. Puntel, É. L. Bolfe, R. J. M. Melchiori, R. Ortega, G. Tiscornia, A. Roel, F. Scaramuzza, S. Best, A. G. Berger, D. S. S. Hansel, D. Palacios Durán, G. R. Balboa

Department of Agronomy and Horticulture: Faculty Publications

Digital agriculture (DA) can contribute solutions to meet an increase in healthy, nutritious, and affordable food demands in an efficient and sustainable way. South America (SA) is one of the main grain and protein producers in the world but the status of DA in the region is unknown. A systematic review and case studies from Brazil, Argentina, Uruguay, and Chile were conducted to address the following objectives: (1) quantify adoption of existing DA technologies, (2) identify limitations for DA adoption; and (3) summarise existing metrics to benchmark DA benefits. Level of DA adoption was led by Brazil and Argentina followed …

Cropland Reference Ecological Unit: A Land Classification Unit For Comparative Soil Studies, Saurav Das, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

There is a growing consensus on a need for measuring the dynamic soil properties of croplands and even comparisons with a reference state or native land. These measurements and paired comparisons will create the capacity to determine soil health management effects and targets. However, the complex soil heterogeneity and climate variations make soil health potential variable and confound the effects of land-use and management practices and comparisons between soils from different sites. Identifying a discrete landmass unit where all soils have similar health potential will be critical in conducting meaningful comparative studies and measuring the impact of conservation practices. This …

A Brief Review Of The Impact Of Silver Nanoparticles On Agriculture And Certain Biological Properties: A Case Study, Sushmashree Krishnappa, Shankramma Kalikeri, Raj Kumar H. Garampalli, Lingaraju H G, Charan Kumar Kachintaya

International Journal of Health and Allied Sciences

Nanotechnology is progressively becoming a popular field of research because it has been successful in changing our agricultural and food systems. According to research published by the UNFAO, agriculture as well as its derivatives would be in high demand sooner or later, owing to nutritional changes. Nanoparticles have been reported to be used in an agricultural sector, because of its capacity to encourage crop growth and yield. Among metal nanoparticles, Silver Nanoparticles (AgNPs) are attracting a lot of attention. We have highlighted some of the agricultural uses of AgNPs, which include pest management, plant disease detection, crop enhancement, and crop …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Australian Cleotychini Planthoppers: Review Of The Genus Cleotyche Emeljanov, 1997 With Three New Species (Hemiptera: Fulgoromorpha: Dictyopharidae), Jérôme Constant, Linda Semeraro, Melinda L. Moir

Biosecurity Research Articles

The genus Cleotyche in the monotypic Australian planthopper tribe Cleotychini (Fulgoroidea: Dictyopharidae) is reviewed. The subgenus Griseotyche subgen. nov. is described to accommodate one species Cleotyche blanda Emeljanov, 2011 while the second species, C. mariae Emeljanov, 1997, is retained in the subgenus Cleotyche (Cleotyche) Emeljanov, 1997. Three new species of Cleotyche (Cleotyche) from Queensland, C. (Cleotyche) christinae sp. nov. from Cania Gorge National Park, C. (Cleotyche) francescoi sp. nov. from Eurimbula National Park and C. (Cleotyche) montana sp. nov. from Blackdown Tableland National Park are described and compared to the …

Determination Of Fungicide Resistance In Botrytis Cinerea On Wine Grapes In California's Central Coast Region, Evelyn Alvarez-Mendoza

Master's Theses

Botrytis bunch rot, caused by Botrytis cinerea, is a fungal disease that primarily affects the fruit of wine grapes. Infection of fruit consequently results in reduced yields and wine quality. These factors lead to significant economic losses for growers which prompts the implementation of management practices to control the disease. One objective of this study was to evaluate the level of resistance that populations of B. cinerea in the Central Coast region showed to various chemicals. A fungicide assay was conducted to determine resistant phenotypes to six fungicide active ingredients (pyrimethanil, iprodione, fenhexamid, fludioxonil, trifloxystrobin, boscalid). Thirty-five (2020) and …

Summaries Of Arkansas Cotton Research 2021, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

While the basic growth and development of the cotton plant have not changed significantly in recent history, the business of cotton production is ever-changing. The last two years have seen us plant a crop just about as late as we thought possible, yet extended favorable conditions at season's end have been our salvation, helping to lead us to record yields. The economic environment over the last few years has been such that farmers need to produce record or near-record yields to advance. Unfortunately, production levels at the state yield average barely cover out-of-pocket expenses.

Great uncertainties exist for the upcoming …

Herbaceous Production Lost To Tree Encroachment In United States Rangelands, Scott L. Morford, Brady W. Allred, Dirac L. Twidwell Jr, Matthew O. Jones, Jeremy D. Maestas, Caleb P. Roberts, David E. Naugle

Department of Agronomy and Horticulture: Faculty Publications

1. Rangelands of the United States provide ecosystem services that benefit society and rural economies. Native tree encroachment is often overlooked as a primary threat to rangelands due to the slow pace of tree cover expansion and the positive public perception of trees. Still, tree encroachment fragments these landscapes and reduces herbaceous production, thereby threatening habitat quality for grassland wildlife and the economic sustainability of animal agriculture.

2. Recent innovations in satellite remote sensing permit the tracking of tree encroachment and the corresponding impact on herbaceous production. We analysed tree cover change and herbaceous production across the western United States …

Adaptation Of Quinoa (Chenopodium Quinoa Willd.) To Australian Environments, Richard Snowball, Harmohinder S. Dhammu, Mario Francesco D'Antuono, David Troldahl, Ian Biggs, Callen Thompson, Mark Warmington, Amanda Pearce, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Quinoa is being evaluated in cropping systems in many countries outside of its natural range of South America. Very few attempts have been made by farmers or researchers to grow or evaluate quinoa under Australian environments. Given the growing popularity of quinoa with consumers, new commercial opportunities for farmers and international interest in the crop, it was timely to undertake a comprehensive evaluation of the potential of quinoa in Australia. Two advanced selections and nine germplasm lines (six of Chilean and three of Bolivian origin) identified in an earlier project were tested in 23 field trials at 14 locations on …

From Free-Energy Profiles To Activation Free Energies, Johannes C. Dietschreit, Dennis J. Diestler, Andreas Hulm, Christian Ochsenfeld, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

Given a chemical reaction going from reactant (R) to the product (P) on a potential energy surface (PES) and a collective variable (CV) discriminating between R and P, we define the free-energy profile (FEP) as the logarithm of the marginal Boltzmann distribution of the CV. This FEP is not a true free energy. Nevertheless, it is common to treat the FEP as the “free-energy” analog of the minimum potential energy path and to take the activation free energy, ΔF‡ _RP, as the difference between the maximum at the transition state and the minimum at R. We show that this …

A Yield Comparison Between Small-Plot And On-Farm Foliar Fungicide Trials In Soybean And Maize, Anabelle Laurent, Emily Heaton, Peter Kyveryga, David Makowski, Laila A. Puntel, Alison E. Robertson, Laura J. Thompson, Fernando Miguez

Department of Agronomy and Horticulture: Faculty Publications

Agronomic research provides management recommendations based on small-plot trials (SPTs) and on-farm trials (OFTs) with very different characteristics. SPTs are traditionally conducted at agricultural experiment stations by research institutes or universities, while OFTs are conducted under commercial-scale conditions and managed by farmers using their own equipment. Several researchers claimed that discrepancies could occur between these two types of trials, which can make the extrapolation of results from SPTs to the farm level difficult. In our study, we conducted an extensive comparison of small-plot and on-farm trials to analyze the effect of foliar fungicide application on maize and soybean yields. We …

Genome Architecture And Diverged Selection Shaping Pattern Of Genomic Differentiation In Wild Barley, Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Yong Jia, Xiao-Qi Zhang, Camilla Hill, Penghao Wang, Brett Chapman, Yong Han, Gaofeng Zhou, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto Barrero, Matthew I. Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li

Grain and Other Field Crops Research Articles

Divergent selection of populations in contrasting environments leads to functional genomic divergence. However, the genomic architecture underlying heterogeneous genomic differentiation remains poorly understood. Here, we de novo assembled two high-quality wild barley (Hordeum spontaneum K. Koch) genomes and examined genomic differentiation and gene expression patterns under abiotic stress in two populations. These two populations had a shared ancestry and originated in close geographic proximity but experienced different selective pressures due to their contrasting micro-environments. We identified structural variants that may have played significant roles in affecting genes potentially associated with well-differentiated phenotypes such as flowering time and drought response between …

Alfalfa Establishment And Management In South Carolina, Liliane Silva, Michael Mrshall, Jeremy Greene, Matias Aguerre

Livestock and Forages

Alfalfa is a perennial legume with high forage quality that can be used under grazing or hay management. This publication outlines establishment and management recommendations for alfalfa plantings in South Carolina.

Snp Discovery In Proso Millet (Panicum Miliaceum L.) Using Lowpass Genome Sequencing, Rituraj Khound, Guangchao Sun, Ravi V. Mural, James C. Schnable, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated ~10,000 years ago in northern China, Proso millet (Panicum miliaceum L.) is a climate-resilient and human health-promoting cereal crop. The genome size of this self-pollinated allotetraploid is 923 Mb. Proso millet seeds are an important part of the human diet in many countries. In the USA, its use is restricted to the birdseed and pet food market. Proso millet is witnessing gradual demand in the global human health and wellness food market owing to its health-promoting properties such as low glycemic index and gluten-free. The breeding efforts for developing improved proso millet cultivars are hindered by the …

The Impact Of Color Of Artificial Led Lighting On Microgreen: A Review, Envry Artanti Duidahayu Putri, Hauzan Ariq Musyaffa Fajri, Firstina Iswari, Fadlan Atalla Muhammad, Rossita Fauziah, Rahmat Budiarto

Jurnal Kultivasi

Microgreen is an emerging agricultural food product that its development can be close type system and rely on the presence of artificial lighting, such as LED. Present study aimed to sum up the artificial LED lighting impact on microgreen production. In general, there were three important variables of light for plant, i.e., light intensity, light duration/photoperiod and light quality. The effect of different LED color on microgreen yield and phytochemical content is also revealed in present review. The red, blue, combination of red and blue, green, white, yellow and UV-A light are numerous choice for optimal microgreen production. However, there …

Karakter Agronomi Beberapa Jagung Hibrida Padjadjaran Dan Hubungannya Dengan Hasil Di Dataran Medium, Yuyun Yuwariah, Desri Nursyahbani Putri, Dedi Ruswandi, Fiky Yulianto Wicaksono, Dhany Esperanza

Jurnal Kultivasi

Budidaya tanaman jagung dengan menggunakan varietas unggul baru dapat memenuhi kebutuhan jagung, baik sebagai pangan maupun pakan dan industri. Karakter agronomi yang dimiliki oleh varietas unggul menjadi kebutuhan evaluasi perakitan kualitas benih. Penelitian ini bertujuan untuk mengetahui karakter agronomi pada jagung hibrida Padjadjaran secara umum maupun masing-masing Jagung Hibrida Padjadjaran yang menentukan hasil. Penelitian ini dilakukan di Sanggar Penelitian, Latihan dan Pengembangan Pertanian (SPLPP) Universitas Padjadjaran di Desa Arjasari, Kecamatan Arjasari, Kabupaten Bandung yang dilakukan sejak 20 Februari hingga 20 Juli 2020. Percobaan ini menggunakan Rancangan Acak Kelompok dan analisis regresi linear berganda dengan 12 perlakuan dan 3 ulangan. Perlakuan …

Effects Of Pesticides On Red Rot Of Sugarcane, Kezia Cristina Da Silva Reis

LSU Master's Theses

Red rot disease of sugarcane, caused by Colletotrichum falcatum, can be an important constraint to crop production. In Louisiana, red rot primarily affects seed-cane and is more severe when billets (stalk sections) are planted rather than whole-stalks. At planting application of seed-treatment chemicals, particularly a combination of fungicide and thiamethoxam insecticide, has improved stand establishment and increased yields in billet plantings in Louisiana. However, information has been lacking on the effect of the treatments on disease development. Greenhouse experiments were conducted in 2020 and 2021 to evaluate stalk rot symptom severity and initial plant growth for billets dip-treated with …

Formulasi Pembawa Rizobakteri Penambat Nitrogen Dan Pelarut Fosfat, Serta Aplikasinya Di Pembibitan Kelapa Sawit Yang Diberi Komposisi Dan Dosis Amelioran Yang Berbeda, Roby Ibnu Syarifain, Mohammad Raihan Rashaun, Eka Dewi Anggrainy, Tualar Simarmata

Jurnal Kultivasi

Keberhasilan aplikasi pupuk hayati berkaitan dengan viabilitas mikroba dalam pembawa, dan aplikasinya di lapangan. Penelitian ini bertujuan mengetahui viabilitas Bakteri Penambat Nitrogen (BPN) dan Bakteri Pelarut Fosfat (BPF) pada berbagai komposisi karier, konsentrasi molase, dan aplikasinya dengan berbagai dosis serta komposisi amelioran pada pembibitan kelapa sawit. Penelitian dilaksanakan dari bulan Oktober 2019 - Mei 2020 di laboratorium dan kebun percobaan Ciparanje. Tahap pertama menggunakan Rancangan Acak Lengkap dua faktor (komposisi karier dan konsentrasi molase) dengan tiga ulangan. Tahap kedua menggunakan rancangan acak kelompok faktorial dua faktor (komposisi amelioran dan dosis aplikasi) pada pembibitan kelapa sawit sebanyak tiga ulangan. Hasil riset …

Effect Of Paclobutrazol On Growth And Root Morphology Of 12 Crossed Stevia In Vitro, Suseno Amien, Qurrota Aini, Noladhi Wicaksana

Jurnal Kultivasi

Stevia (Stevia rebaudiana Bertoni) is a low-calorie alternative sweetener. The superior varieties of Stevia in Indonesia are limited. Availability of seeds plays an important role in the Stevia plant breeding program for both the selection stage and the production of high-yielding varieties. Tissue culture is the best method of Stevia propagation, but the low adaptability of plantlets causes death in the acclimatization phase. The growth and root morphology of 12 crossbreed Stevia were evaluated. A Completely Randomized Design was used with factorial consisting of two factors were Stevia genotypes (STG1,7,8,10, SBG3,4,5,7,10, SGB2,3, and SBT11) and the plant growth regulator (PGR) …

Uji Kemampuan Bakteri Diazotrof Asal Perakaran Bawang Merah Dalam Mendukung Pertumbuhan Dan Hasil Tanaman Bawang Merah, Ananda Rahmalia Yuniarti, Eny Rokhminarsih, Purwanto

Jurnal Kultivasi

Budidaya tanaman bawang merah saat ini dihadapkan pada masalah stagnasi produksi akibat aplikasi pupuk kimia secara berlebihan sehngga efisiensi pemupukan rendah. Teknologi ramah lingkungan dan efisien pupuk dengan aplikasi bioteknologi melalui aplikasi bakteri berguna seperti bakteri penambat nitrogen. Penelitian bertujuan untuk (1) mengetahui respons pertumbuhan dan hasil tanaman bawang merah terhadap bakteri diazotrof, (2) menetapkan isolat bakteri diazotrof yang paling baik untuk pertumbuhan dan hasil tanaman bawang merah. Percobaan dilaksanakan pada bulan Januari 2021 sampai April 2021. Percobaan dilaksanakan di Rumah Kassa serta Laboratorium Agronomi dan Hortikultura Fakultas Pertanian, Universitas Jenderal Soedirman. Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok …