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Physiological Host Range Of Ceratapion Basicorne, A Prospective Biological Control Agent Of Centaurea Solstitialis (Asteraceae), Lincoln Smith

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ceratapion basicorne (Coleoptera: Apionidae) is a weevil native to Europe and western Asia that is being evaluated as a prospective classical biological control agent of Centaurea solstitialis (yellow starthistle) in the United States. Host plant specificity of the insect was evaluated in no-choice oviposition experiments. Feeding on leaf tissue by adult females was highly correlated to oviposition rate, both of which occurred primarily on plants in the tribe Cardueae, and especially those in the monophyletic subtribe Centaureinae. The highest rates of larval development occurred on Ce. solstitialis and Centaurea cyanus (bachelor’s button, garden cornflower), and there was significant development on …

The Cercospora Nicotianae Gene Encoding Dual O-Methyltransferase And Fad-Dependent Monooxygenase Domains Mediates Cercosporin Toxin Biosynthesis, Katherine L. Dekkers, Bang-Jau You, Vivek S. Gowda, Hui-Ling Liao, Miin-Huey Lee, Huey-Jiunn Bau, Peter P. Ueng, Kuang-Ren Chung

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cercosporin, a photo-activated, non-host-selective phytotoxin produced by many species of the plant pathogenic fungus Cercospora, causes peroxidation of plant cell membranes by generating reactive oxygen species and is an important virulence determinant. Here we report a new gene, CTB3 that is involved in cercosporin biosynthesis in Cercospora nicotianae. CTB3 is adjacent to a previously identified CTB1 encoding a polyketide synthase which is also required for cercosporin production. CTB3 contains a putative O-methyltransferase domain in the N-terminus and a putative flavin adenine dinucleotide (FAD)-dependent monooxygenase domain in the C-terminus. The N-terminal amino acid sequence also is similar to …

Genetic Diversity In Laboratory Colonies Of Western Corn Rootworm (Coleoptera: Chrysomelidae), Including A Nondiapause Colony, Kyung Seok Kim, B. Wade French, Douglas V. Sumerford, Thomas W. Sappington

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Laboratory-reared western corn rootworms, Diabrotica virgifera virgifera, from colonies maintained at the North Central Agricultural Research Laboratory (NCARL) in Brookings, SD, are used extensively by many researchers in studies of the biology, ecology, behavior, and genetics of this major insect pest. A nondiapause colony developed through artificial selection in the early 1970s is particularly attractive for many studies because its generation time is much shorter than that of typical diapause colonies. However, the nondiapause colony has been in culture for ≈190 generations without out-crossing. We compared variation at six microsatellite loci among individuals from the NCARL nondiapause colony (≈190 …

The Influence Of Oscillating Dietary Protein Concentrations On Finishing Cattle. Ii. Nutrient Retention And Ammonia Emissions, S. L. Archibeque, H. C. Freetly, N. A. Cole, Calvin Ferrell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We hypothesized that oscillation of the dietary CP concentrations would improve efficiency of N use and reduce N loss to the environment. Charolais-cross steers (n = 8; 315 ± 21 kg of BW) were used in a replicated 4 × 4 Latin square design. The steers were allowed ad libitum access to the following diets: 1) 9.1% CP (low), 2) 11.8% CP (medium), 3) 13.9% CP (high), or 4) low and high oscillated on a 48-h interval on each diet (oscillating). Dry matter intake did not differ among treatments (P = 0.46), but N intake differed (P < 0.01) from 94 (low) to 131 (medium), 142 (high), and 133 g/d (oscillating), as designed. Dry matter digestibility increased (P < 0.01) from 71.8% (low) to 75.8% (medium), 77.7% (high), and 77.5% (oscillating). Nitrogen digestibility increased (P < 0.01) from 62.2% (low) to 67.2% (medium) to 70.1% (high) and 70.9% (oscillating). Nitrogen retention was greater (P < 0.01) in steers fed oscillating (55.0 g/d) than in the steers fed low (34.8 g/ d) or high (40.2 g/d), but N retention of steers fed medium (49.8 g/d) differed (P = 0.02) only from that of steers fed low. Urinary urea N did not differ between steers fed medium (19.5 g/d) or oscillating (21.3 g/d) but was lowest (P < 0.01) for those fed low (8.2 g/d) and greatest for those fed high (39.2 g/d). Daily heat production (kcal/BW^0.75) tended …

Early Investments In Soil Conservation Research Continues To Provide Dividends, J. E. Gilley, D. C. Flanagan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Current soil conservation programs are built upon an established research legacy. Hugh H. Bennett, chief of the USDA Soil Conservation Service from 1935 to 1951, was instrumental in the establishment of a network of 35 soil conservation experiment stations (SCES). Research projects were initiated at the SCES in the 1930s to investigate the principal factors causing erosion and to identify the most effective and practical methods of controlling soil loss from agricultural areas. Information obtained from the SCES, and selected other locations, was assembled at the National Runoff and Soil Loss Data Center (NRSLDC) established on the campus of Purdue …

Environmental Abundance Of Anopheles (Diptera: Culicidae) Larval Habitats On Land Cover Change Sites In Karima Village, Mwea Rice Scheme, Kenya, Benjamin G. Jacob, Ephantus Muturi, Patrick Halbig, Joseph Mwangangi, R. K. Wanjogu, Enock Mpanga, Jose Funes, Josephat Shililu, John Githure, James L. Regens, Robert J. Novak

All Peer-Reviewed Publications

A study was carried out at Karima Village in the Mwea Rice Irrigation Scheme in Kenya to assess the impact of rice husbandry and associated land cover change for mosquito larval abundance. A multi-temporal, land use land cover (LULC) classification dataset incorporating distributions of Anopheles arabiensis aquatic larval habitats was produced in ERDAS Imagine version 8.7 using combined images from IKONOS at 4m spatial resolution from 2005 and Landsat Thematic Mapper (TM)™ classification data at 30-meters spatial resolution from 1988 for Karima. Of 207 larval habitats sampled, most were either canals (53.4%) or paddies (45.9%), and only one habitat was …

Larval Habitat Dynamics And Diversity Of Culex Mosquitoes In Rice Agro-Ecosystem In Mwea, Kenya, Ephantus J. Muturi, Josephat I. Shililu, Weidong Gu, Benjamin G. Jacob, John I. Githure, Robert J. Novak

All Peer-Reviewed Publications

Introduction of irrigation projects in developing nations has often been blamed for aggravating the problem of mosquito-borne diseases by creating ideal larval habitats for vector mosquitoes. However, whereas several studies have demonstrated the relationship between malaria vectors and irrigation, little work has been done on culicine mosquitoes despite their potential in transmission of filariasis and arboviruses and their significant biting nuisance in these areas. This study examined the diversity of Culex mosquito fauna and their larval habitats at two sites (Murinduko and Kiamachiri) in Mwea, Kenya over a 12-month period. The habitat types present at each site within a 200-meter …

Antibacterial Activity Of Apis Mellifera L. Propolis Collected In Three Regions Of Kenya, Elliud M. Muli, J. M. Maingi

All Peer-Reviewed Publications

The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted using four different concentrations of ethanol: pure, 70%, 50%, and 30%. Ethanol (70%) and Streptomycin were used as controls. The agar diffusion method using filter paper disks was employed. Antibacterial activity was determined as an equivalent of the inhibition zones diameters (in millimeters) after incubation at 37°C for 24h. Significant differences in the antibacterial activities of propolis were observed among …

Switchgrass For Biomass Feedstock In The Usa, Matt Sanderson, Paul Adler, Akwasi Boateng, Michael Casler, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass has received much study for biomass feedstock production and conversion through research funded by the US-DOE and USDA during the past two decades. We have an improved understanding of the adaptation of existing cultivars and new cultivars with yield and adaptation improvements are now appearing. We also have a good understanding of the agronomics of switchgrass production, mainly from its use as a forage crop. There remain several constraints to switchgrass use in bioenergy cropping systems, including reliable establishment methods to obtain productive stands in the first year, targeted fertilization and nutrient management techniques to efficiently use nitrogen fertilizer, …

Farmers' Perceptions Of Importance, Control Practices And Alternative Hosts Of Maize Stemborers In Uganda, T. Kalule, Z. R. Khan, G. Bigirwa, J. Alupo, S. Okanya, J. A. Pickett, L. J. Wadhams

All Peer-Reviewed Publications

A survey was conducted in Tororo, Bugiri and Kapchorwa districts in eastern and northeastern Uganda on farmers' perceptions of constraints in maize production. Farmers in Tororo and Bugiri consistently ranked stemborers and striga as the major production constraints in maize growing, while those in Kapchorwa ranked stemborers and lack of capital as the most important. Approximately 35 and 40% of the farmers in Tororo and Bugiri, respectively, identified stemborers as the 'caterpillar' that destroys their maize. In Kapchorwa, 50% of the farmers identified stemborers. Pesticide application was the most common control measure while the use of indigenous and cultural control …

The Circuitous Path To The Comparison Of Simulated Values From Crop Models With Field Observations, Albert Weiss, Wally Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Journal of Agricultural Science, Cambridge has been a fixture in dissemination of crop simulation models and the concepts and data upon which they are built since the inception of computers and computer modeling in the mid-20th century. To quantify the performance of a crop simulation model, model outputs are compared with observed values using statistical measures of bias, i.e. the difference between simulated and observed values. While applying these statistical measures is unambiguous for the experienced user, the same cannot always be said of determining the observed or simulated values. For example, differences in accessing crop development can be …

Convergence Of Agriculture And Energy: Implications For Research And Policy, Kenneth Cassman, Vernon Eidman, Eugene Simpson, Larry Berger, Robert Loomis, Marie Walsh, Todd A. Peterson, Edward Runge

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Access to an adequate energy supply at reasonable cost is crucial for sustained economic growth. Unfortunately, oil prices and the need to import from politically unstable countries lowers the reliability of the U.S. energy supply and hinders economic development. Although biofuels have been identified as an important component of the national strategy to decrease U.S. dependence on imported oil, the ability to sustain a rapid expansion of biofuel production capacity raises new research and policy issues. This document seeks to identify the most critical of these issues to help inform the policy development process. The goal is to enhance the …

Intsormil Crsp: Sorghum, Millet And Other Grains Collaborative Research Support Program: Leader With Associates Cooperative Agreement No. Eep-A-00-06-0016-00; Award From The U.S. Agency For International Development To The University Of Nebraska-Lincoln, Joseph Schmidt

INTSORMIL Scientific Publications

Pursuant to the authority contained in the Foreign Assistance Act of 1961, as amended, the U.S. Agency for International Development (USAID) hereby awards to University of Nebraska - Lincoln (hereinafter referred to as the "Recipient"), the sum of $9,000,000.00 to provide support for a program in Sorghum, Millet, and Other Grains Collaborative Research Support Program as described in the Schedule of this award and in Attachment B, entitled "Program Description."

This Leader with Associates Cooperative Agreement is effective and obligation is made as of 09-30-2006 and shall apply to expenditures made by the Recipient in furtherance of program objectives during …

Isolation Of An Active Lv1 Gene From Cattle Indicates That Tripartite Motif Protein-Mediated Innate Immunity To Retroviral Infection Is Widespread Among Mammals, Laura M.J. Ylinen, Zuzana Keckesova, Benjamin L.J. Webb, Robert J.M. Gifford, Timothy P.L. Smith, Greg J. Towers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Lv1/TRIM5α (tripartite motif 5α) has recently emerged as an important factor influencing species-specific permissivity to retroviral infection in a range of primates, including humans. Old World monkey TRIM5α blocks human immunodeficiency virus type 1 (HIV-1) infectivity, and the human and New World monkey TRIM5α proteins are inactive against HIV-1 but active against divergent murine (N-tropic murine leukemia virus [MLV-N]) and simian (simian immunodeficiency virus from rhesus macaque [SIVmac]) retroviruses, respectively. Here we demonstrate antiviral activity of the first nonprimate TRIM protein, from cattle, active against divergent retroviruses, including HIV-1. The number of closely related human TRIM sequences makes assignment of …

A Matter Of Balance: Conservation And Renewable Energy, Jane M.F. Johnson, Don Reicosky, Ray Allmaras, Dave Archer, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

As communities and a country, we have a monumental task to solve the energy and global climate change problems, while maintaining our capacity to produce food, feed, and fiber for an ever increasing world population. The severity of these problems is exacerbated by the universal desire for an increased standard of living, which invariably translates to more energy use, greater demand for products, and higher quality diets (usually in the form of more fresh fruits and vegetables and more animal protein). Agriculture and forestry are in a unique position as we attempt solve these opposing problems in the most beneficial …

Slides: Climate Change & The Ecological Resources Of The West, Chris Field

Climate Change and the Future of the American West: Exploring the Legal and Policy Dimensions (Summer Conference, June 7-9)

Presenter: Chris Field, Director, Department of Global Ecology, Carnegie Institution of Washington, DC.

39 slides.

Contains references.

Agenda: Climate Change And The Future Of The American West: Exploring The Legal And Policy Dimensions, University Of Colorado Boulder. Natural Resources Law Center

Climate Change and the Future of the American West: Exploring the Legal and Policy Dimensions (Summer Conference, June 7-9)

Sponsors: The William and Flora Hewlett Foundation; BP America; Holland & Hart; Patrick, Miller & Krope, P.C.; The Rocky Mountain Mineral Law Foundation, Rocky Mountain Natural Resource Center of the National Wildlife Federation, Western Water Assessment.

Exploring the legal and political dimensions that climate change will bring to the American West will be the focus of the CU-Boulder Natural Resources Law Center's 27th Annual Summer Conference.

Titled "Climate Change and the Future of the American West: Exploring the Legal and Policy Dimensions," the conference will be held June 7-9 at the Fleming Law Building on the University of Colorado at …

Slides: The Real Biofuel Cycles And The Earth, Biofuels, And Reality, Tad W. Patzek

Climate Change and the Future of the American West: Exploring the Legal and Policy Dimensions (Summer Conference, June 7-9)

Presenter: Tad W. Patzek, Professor of Petroleum Engineering, University of California at Berkeley, Berkeley, CA

2 pages and 29 slides.

Contains footnotes.

The Earth, Energy, And Agriculture, Tad W. Patzek

Climate Change and the Future of the American West: Exploring the Legal and Policy Dimensions (Summer Conference, June 7-9)

Presenter: Tad W. Patzek, Professor of Petroleum Engineering, University of California at Berkeley, Berkeley, CA.

13 pages (includes some color illustrations).

Contains references.

Nitric Oxide Accelerates Seed Germination In Warm-Season Grasses, Gautam Sarath, Paul C. Bethke, Russell Jones, Lisa M. Baird, Guichuan Hou, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The nitric oxide (NO) donor sodium nitroprusside (SNP) significantly promoted germination of switchgrass (Panicum virgatum L. cv Kanlow) in the light and in the dark at 25°C, across a broad range of concentrations. SNP also promoted seed germination in two other warm-season grasses. A chemical scavenger of NO inhibited germination and blocked SNP stimulation of seed germination. The phenolic (+)-catechin acted synergistically with SNP and nitrite in promoting seed germination. Acidified nitrite, an alternate NO donor also significantly stimulated seed germination. Interestingly, sodium cyanide, potassium ferricyanide and potassium ferrocyanide at 200 lM strongly enhanced seed germination as well, whereas …

Influence Of Soil Compaction On Nitrogen Volatilization In A Management Intensive Grazing System: Estimation Of Gaseous N Losses Using Mass Balance In Intact Soil Cores, Luke Alan Petersen

Undergraduate Honors Capstone Projects

Increasing concern about the environmental impacts of greenhouse gases and PM 2.5 particulates has prompted many researchers to examine the processes of gaseous loss of nitrogen (N) from agricultural land. As agricultural production becomes more competitive and producers strive to become more efficient by reducing input costs, they will increasingly employ practices such as the rotational stocking, also called Management Intensive Grazing (MIG). MIG utilizes high animal stocking rates for short periods of time to efficiently harvest pasture crops. Unfortunately, MIG also produces relatively high concentrations of livestock excreta. This has caused intensive grazing practices to become a focal point …

The Arabidopsis Homolog Of Trithorax, Atx1, Binds Phosphatidylinositol 5-Phosphate, And The Two Regulate A Common Set Of Target Genes, Raul Alvarez-Venegas, Monther Sadder, Andrej Hlavacka, Frantisek Baluska, Yuannan Xia, Guoqing Lu, Alexey Firsov, Gautam Sarath, Hideaki Moriyama, Joseph G. Dubrovsky, Zoya V. Avramova

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

TheArabidopsis homolog of trithorax, ATX1, regulates numerous functions inArabidopsis beyond the homeotic genes. Here, we identified genome-wide targets of ATX1 and showed that ATX1 is a receptor for a lipid messenger, phosphatidylinositol 5-phosphate, PI5P. PI5P negatively affects ATX1 activity, suggesting a regulatory pathway connecting lipid-signaling with nuclear functions. We propose a model to illustrate how plants may respond to stimuli (external or internal) that elevate cellular PI5P levels by altering expression of ATX1-controlled genes.

Includes supplemental supporting information.

Lysine Residues In N-Terminal And C-Terminal Regions Of Human Histone H2a Are Targets For Biotinylation By Biotinidase, Yap Ching Chew, Gabreale Camporeale, Nagarama Kothapalli, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In eukaryotic cell nuclei, DNA associates with the core histones H2A, H2B, H3 and H4 to form nucleosomal core particles. DNA binding to histones is regulated by posttranslational modifications of N-terminal tails (e.g., acetylation and methylation of histones). These modifications play important roles in the epigenetic control of chromatin structure. Recently, evidence that biotinidase and holocarboxylase synthetase (HCS) catalyze the covalent binding of biotin to histones has been provided. The primary aim of this study was to identify biotinylation sites in histone H2A and its variant H2AX. Secondary aims were to determine whether acetylation and methylation of histone H2A affect …

Genic Microsatellite Markers Derived From Est Sequences Of Switchgrass (Panicum Virgatum L.), Christian M. Tobias, Daniel M. Hayden, Paul Twigg, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass is a large, North American, perennial grass that is being evaluated as a potential energy crop. There is a need to assess genetic diversity in stored accessions and in remaining native stands to assist breeding and conservation efforts. Marker development will also be necessary for genetic linkage mapping. Toward this end, 32 switchgrass genic di-, tri- and tetranucleotide repeat microsatellites were identified from expressed sequence tags (ESTs). These microsatellites were used to screen individuals from two different named cultivars. The markers displayed a high level of polymorphism consistent with the tetraploid, allogamous behaviour of the cultivars tested.

The Established Intimin Receptor Tir And The Putative Eucaryotic Intimin Receptors Nucleolin And B1 Integrin Localize At Or Near The Site Of Enterohemorrhagic Escherichia Coli O157:H7 Adherence To Enterocytes In Vivo, James F. Sinclair, Evelyn A. Dean-Nystrom, Alison D. O’Brien

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

For enterohemorrhagic Escherichia coli (EHEC) O157:H7 to adhere tightly to the intestinal epithelium and produce attach and efface (A/E) lesions, the organism must express the adhesin intimin and insert the bacterially encoded translocated intimin receptor Tir into the plasma membrane of the host enterocyte. Additionally, some reports based on tissue culture experiments indicate that intimin has affinity for the eucaryotic proteins nucleolin and β1 integrin. To address the potential biological relevance of these eucaryotic proteins in the infection process in vivo, we sought to compare the proximity of Tir, nucleolin, and β1 integrin to regions of EHEC …

Chinch Bug (Hemiptera: Blissidae) Mouthpart Morphology, Probing Frequencies, And Locations On Resistant And Susceptible Germplasm, Wyatt G. Anderson, Tiffany Heng-Moss, Frederick P. Baxendale, Lisa M. Baird, Gautam Sarath, Leon G. Higley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chinch bugs are common pests of many agronomic and horticulturally important crops and turfgrasses. Previous research has indicated that some grasses exhibit resistance to multiple chinch bug species, whereas others are resistant to only one species. The objectives of this research were to document differences in the probing frequencies and locations among Blissus species as well as differences in mouthpart morphology as a first step in understanding the differential responses of grasses to chinch bug feeding. Scanning electron microscopy detected differences in the total lengths of proboscises as well as individual mouthpart segments among the four species studied. Blissus occiduus …

Transport Of E. Coli In Egyptian Agricultural Soils As A Result Of Reclaimed Wastewater Use For Irrigation, Aimen Badawy

Archived Theses and Dissertations

Limited water resources are one of the main constraints to socio-economic development and even a source of insecurity at national and regional levels. The increasing demand for water in the agricultural sector stimulated the use of reclaimed wastewater for irrigation. Comprehending health and environment risks resulting from contaminants that may often be associated with reclaimed wastewater will lead to the sustainable use of this resource, as well as the conservation of other water resources.

In the present study the fate and transport of one important wastewater constituent, namely E. Coli bacteria, in three Egyptian soils of typical agricultural use was …

Evaluation Of Cauliflower And Broccoli Varieties: Summer Planting (November 2005 - February 2006), Department Of Food And Agriculture, Western Australia

Horticulture research reports

This document has been prepared from a cauliflower and broccoli variety evaluation trial conducted at the Manjimup Horticultural Research Institute, South Western Highway, Manjimup WA 6258. The results reflect the growth characteristics, yield, quality and density of the cauliflower and broccoli varieties as recorded at this site during the conditions of the growing period (November 2005 to February 2006).

This report is the first in a series of four produced from the evaluation of cauliflower and broccoli varieties conducted at the Manjimup Horticultural Research Institute.

The results are provided to allow seed suppliers, seedling nurseries, producers, packers and marketers to …

Australian Temperate Strawberry Breeding Program (Western Australia) Selection Of New And Advanced Short Day Breeding Lines And Cultivars In Western Australia - 2005/2006, Michaela Antoine, Dennis Phillips

Horticulture research reports

Sixty-eight 'short day' selections from the Australian Temperate breeding program were compared in a trial planting on a commercial property at Wanneroo from a mid May planting. Thirty-three of the selections were derived from crosses made in 2003 (new Stage 4 selections) and twenty-nine were from crosses made between 1998 and 2002 (Intermediate Stages 5 and 6). In addition, three advanced selections were compared in large plots of 1,000-1 ,500 plants together with three commercial control cultivars, Gaviota, Camino Real and Kiewa. These selections were 'fast tracked' for future commercialisation by being selected for bulking up in September 2004.

Seven …

Natural Abundance Of Foliar 15N As An Early Indicator Of Nitrogen Deficiency In Fertilized Cotton, S. Stamatiadis, C. Christofides, C. Tsadilas, V. Samaras, James S. Schepers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Information on the contribution of various soil nitrogen (N) sources to plant N uptake is often needed for the implementation of sustainable or site-specific management practices in agriculture. Considering the limitations of traditional methods in meeting these needs, this study investigated the potential of leaf δ¹⁵N as an early indicator of nutrient deficiency in cotton. The spatial and temporal natural abundance of ¹⁵N was measured in the soil and leaves of a fertilized cotton field located near the village of Moschochori (Larissa, Greece). The isotopic signal of the leaves was interpreted in the context of the relative …