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Evaluation Of Oviposition Substrates And Organic Infusions On Collection Of Culex In Florida, Sandra Allan, Ulrich R. Bernier, David Kline

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Gravid mosquito traps are commonly used for both arbovirus surveillance and population surveillance of mosquitoes of the genus Culex. Oviposition substrates, used as baits in these traps, were tested against Culex under laboratory and field conditions. In the laboratory all substrates tested as 1% and 10% dilutions in 2-choice bioassays against female Cx. quinquefascians were significantly more effective than well water controls in eliciting oviposition. Strongest responses were to dilutions of dairy effluent, followed by larval water and infusions of alfalfa hay, alfalfa pellets, Bermuda hay, oak leaves, and Typha leaves, with lowest responses to cow manure infusion. …

Influence Of Dietary Crude Protein Concentration And Source On Potential Ammonia Emissions From Beef Cattle Manure, N. A. Cole, R. N. Clark, R. W. Todd, C. R. Richardson, A. Gueye, L. W. Greene, K. Mcbride

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Emissions of ammonia, as well as other gases and particulates, to the atmosphere are a growing concern of livestock producers, the general public, and regulators. The concentration and ruminal degradability of CP in beef cattle diets may affect urinary and fecal excretion of N and thus may affect ammonia emissions from beef cattle feed yards. To determine the effects of dietary CP concentration and degradability on potential ammonia emissions, 54 steers were randomly assigned to nine dietary treatments in a 3 × 3 factorial arrangement of treatments. Treatments consisted of three dietary CP concentrations (11.5, 13, and 14.5%) and three …

Involvement Of Acetosyringone In Plant–Pathogen Recognition, C. Jacyn Baker, Norton M. Mock, Bruce D. Whitaker, Daniel P. Roberts, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study, acetosyringone was identified as one of the major extracellular phenolics in tobacco suspension cells and was shown to have bioactive properties that influence early events in plant–bacterial pathogenesis. In our model system, tobacco cell suspensions treated with bacterial isolate Pseudomonas syringae WT (HR+) undergo a resistant interaction characterized by a burst in oxygen uptake several hours after inoculation. When the extracellular concentration of acetosyringone in tobacco cell suspensions was supplemented with exogenous acetosyringone, the burst in oxygen uptake occurred as much as 1.5 h earlier. The exogenous acetosyringone had no effect on tobacco suspensions undergoing susceptible interactions …

Induction Of Redox Sensitive Extracellular Phenolics During Plant–Bacterial Interactions, C. Jacyn Baker, Bruce D. Whitaker, Daniel P. Roberts, Norton M. Mock, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study focuses on the transient and complex nature of phenolics that accumulate in the extracellular environment of plant suspension cells during the first few hours of the interaction between these plant cells and bacterial pathogens. Using suspension cells of Nicotiana tabacum we identified four acetophenones and four hydroxycinnamic acid amides that accumulate in this extracellular environment. Treatment of the suspension cells with isolates of the plant pathogen Pseudomonas syringae or heat-killed bacteria increased elicitation of extracellular phenolics and changed the composition of the compounds that accumulated. These phenolics were sensitive to oxidative stress; when suspension cells were treated with …

Lamb Production Of Dorper, Katahdin, And St. Croix Bred In Summer, Winter, Or Spring In The Southeastern United States, J. M. Burke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ewe production traits and ability to breed out of season were compared for the Dorper (DO), Katahdin (KA), and St. Croix (SC) breeds between 2000 and 2005. Sheep were managed on grass pasture and were supplemented with corn/soybean meal and free-choice, trace-mineral mix. Ewes were exposed to rams of their respective breeds in late summer (August/September), winter (December), or spring (April/ May) for 30-day breeding periods. Lambs were weighed at birth and 60 days of age. Pregnancy and lambing rates and litter birth weight were greater for all breeds bred in winter and lowest in spring. Pregnancy losses were greater …

Growth Performance Of Barbados Blackbelly, Katahdin And St. Croix Hair Sheep Lambs Fed Pasture- Or Hay-Based Diets, S. Wildeus, K. E. Turner, J. R. Collins

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two experiments evaluated growth of mixed-sex Barbados Blackbelly, Katahdin, and St. Croix hair sheep lambs raised on pasture or hay-based diets with moderate levels of energy supplementation. In Experiment 1, 36 ewe and wether lambs were allocated to a pasture or pen feeding group in May. Pasture animals rotationally grazed tall fescue pasture, while pen animals were offered chopped alfalfa hay, and both groups were supplemented with corn/soybean meal at 0.75% of body weight. In Experiment 2, 72 lambs were allocated to pen and pasture in April, and provided either a low or high crude protein concentration corn/soybean meal supplement …

Composition Of Irrigation Water Salinity Affects Growth Characteristics And Uptake Of Selenium And Salt Ions By Soybean, D. Wang, C. M. Grieve, D. L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A greenhouse study was conducted to determine the effects of irrigation waters differing in salt composition on growth characteristics, salt ion and selenium (Se) accumulation, and distribution in plant components of the soybean (Glycine max L. Merr.) cultivar “Manokin.” Plants were grown in sand cultures and irrigated with isoosmotic solutions containing (1) Cl⁻ as the dominant anion, or (2) a mixture of salts containing equal molar amounts of SO_^2-4  and Cl⁻. Six treatments of each salinity type were imposed. Electrical conductivities of the irrigation waters ranged from 2.1 to 13.0 dSm⁻¹. Selenium …

B. R. Wells Rice Research Studies 2003, R. J. Norman, J.-F. Meullenet, K. A.K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice-producing state in the U.S., representing just over 48% of the total U.S. production and 48.5% of the total acres planted to rice. Rice cultural practices vary across the state and across the U.S. However, due to changing political, environmental, and economic times, the practices are dynamic. This survey was initiated in 2002 to monitor how the changing times reflect the changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas where rice is produced. Questions included topics such as …

Multi-Trophic Level Interactions In A Cassava-Maize Mixed Cropping System In The Humid Tropics Of West Africa, F. Schulthess, A. Chabi-Olaye, S. Gounou

All Peer-Reviewed Publications

Multi-trophic level interactions in a mixed crop, involving cassava and maize, were studied in derived-savanna in Benin, West Africa. Two trials were planted, one during the short rainy season two months before onset of the dry season and one during the long rainy season in spring. Key pests under study on maize were the noctuid Sesamia calamistis Hampson and the pyralids Eldana saccharina Walker and Mussidia nigrivenella Ragonot, and on cassava, the exotic mealybug, Phenacoccus manihoti Matile-Ferrero and its encyrtid parasitoid Apoanagyrus lopezi De Santis. Both crops received insecticide treatments to assess the crop loss by a pest species. On …

K8 And K12 Are Biotinylated In Human Histone H4, Gabriela Camporeale, Elizabeth E. Shubert, Gautam Sarath, Ronald Cerny, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Folding of DNA into chromatin is mediated by binding to histones such as H4; association of DNA with histones is regulated by covalent histone modifications, e.g. acetylation, methylation, and biotinylation.We sought to identify aminoacid residues that are biotinylated in histone H4,and to determine whether acetylation and methylation of histones affect biotinylation. Synthetic peptides spanning fragments of human histone H4 were biotinylated enzymatically using biotinidase. Peptide-bound biotin was probed with streptavidin– peroxidase. Peptides based on the N-terminal sequence of histone H4 were effectively recognized by biotinidase as substrates for biotinylation; in contrast, peptides based on the C-terminal sequences were not biotinylated. …

National Science Foundation-Sponsored Workshop Report. Draft Plan For Soybean Genomics, Gary Stacey, Lila N Vodkin, Wayne A. Parrott, Randy C. Shoemaker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Recent efforts to coordinate and define a research strategy for soybean (Glycine max) genomics began with the establishment of a Soybean Genetics Executive Committee, which will serve as a communication focal point between the soybean research community and granting agencies. Secondly, a workshop was held to define a strategy to incorporate existing tools into a framework for advancing soybean genomics research. This workshop identified and ranked research priorities essential to making more informed decisions as to how to proceed with large scale sequencing and other genomics efforts. Most critical among these was the need to finalize a physical …

Cattle As Grazing Management And Seed Dispersal Tools For Increasing Native Species Diversity On Great Basin Rangelands, Marina K. Whitacre

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A series of experiments evaluated: 1) the influence of seed intake and gut retention time on seed passage, recovery, and germinability; 2) fecal seeding and broadcast /trampling as techniques to incorporate seeds into a well-established Agropyron desertorum (Fisch.) Schult. stand in Skull Valley , Utah; 3) intensive grazing as a means to reduce Agropyron biomass and increase establishment and survival of seeded species; and 4) the recovery and germinability of seed extracted from dung collected from the field. Two shrubs (Artemisia tridentata Nutt. ssp. wyomingensis Beetle & Young and Atriplex confertifolia Torr. & Frem.), a grass (Elymus elymoides (Raf.) …

Intsormil 2004 Annual Report, John M. Yohe, Thomas Crawford Jr., Kimberly Christiansen, Joan Frederick

INTSORMIL Impacts and Bulletins

The 2004 INTSORMIL Annual Report presents the progress and notable achievements by the SorghumlMillet CRSP during the period of July 1, 2003 - June 30, 2004. These results are an outcome of partnerships between scientists at six U.S. Land Grant Universities (Kansas State University, Mississippi State University, University of Nebraska, Purdue University, Texas A&M University and West Texas A&M University) and scientists of the Agricultural Research Service of the U.S. Department of Agriculture at Tifton, Georgia and National Agricultural Research Systems (NARS) and National Universities in nineteen countries in Central America, West Africa, East Africa and Southern Africa.

Agricultural research …

Pest Management Benefits Of Compost Mulch In Apple Orchards, M. W. Brown, Thomas Tworkoski

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effect of compost application on weed, fungal, and insect pest management in apple orchards was investigated from 1999 to 2001. Composted poultry manure was applied in June 1999 to half of two small research orchards which had previously received little or no management. The compost provided weed control for 1 year after application. There was no effect of compost on apple scab (Venturia inaequalis) infection. In a laboratory experiment, growth of the brown rot fungus (Monilinia fructicola) was significantly slower on a compost substrate than a sterilized compost substrate. The compost significantly affected arthropod abundance …

Using Multi-Spectral Imagery To Evaluate Corn Grown Under Nitrogen And Drought Stressed Conditions, Shannon L. Osborne, James S. Schepers, Mike R. Schlemmer

Department of Agronomy and Horticulture: Faculty Publications

The in-season estimation of crop stresses which have the potential of adversely affecting crop yield and/or quality could allow producers to make in-season management decisions to correct for the particular stress. A field study was conducted to evaluate the use of multispectral imagery for estimating corn (Zea mays L.) grain yield, in-season biomass and nitrogen (N) concentration under varying N and drought stresses. The experiment was a split-plot design with three replications using a factorial arrangement of treatments. Three irrigation (whole-plot) treatments included dry land, irrigation based on 0.5, and full evapotranspiration (ET). Sub-plot treatments included five N …

Levels And Changes Of Soil Phosphorus In Subtropical Beef Cattle Pastures, Gilbert C. Sigua, Mary J. Williams, Samuel W. Coleman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Long-term pasture management is believed to change soil chemical properties, but little is known about whether pasture management, such as fertilizer application, grazing, or haying can initiate such change in sandy and well-drained subtropical beef pastures. The objective of this study was to investigate the long term effect of pasture management (grazing+haying, GZ+HY) on soil phosphorus (P) dynamics (levels and changes) in subtropical beef cattle pastures with bahiagrass (BG, Paspalum notatum) and rhizoma peanut (RP, Arachis glabrata) with (WP) or without (WNP) P fertilization in Brooksville, FL from 1988 to 2000. Soil P dynamics in Subtropical Agricultural Research …

Habitat Manipulation Of The Apple Orchard Floor To Increase Ground-Dwelling Predators And Predation Of Cydia Pomonella (L.) (Lepidoptera: Tortricidae), Clarissa R. Mathews, Dale G. Bottrell, M. W. Brown

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Augmenting generalist predator populations in new apple (Malus domestica Bork) plantings could potentially aid in the establishment of balanced orchard ecosystems that are less susceptible to pest outbreaks. Habitat can be an important factor in retaining predators in a system and can affect predator efficiency. We investigated the potential of increasing a complex of generalist ground-dwelling predators and enhancing biological control in a young 'Golden Delicious' apple orchard through ground habitat manipulation. We modified the orchard floor with four comparative habitat treatments: (1) detritus-rich compost mulch layer, (2) detritus-free synthetic mulch layer, (3) herbicide-treated vegetation thatch, and (4) bare …

Molecular Mapping And Identification Of Soybean Fatty Acid Modifier Quantitative Trait Loci, D. L. Hyten Jr., Vincent R. Pantalone, Arnold M. Saxton, Michael E. Schmidt, Carl E. Sams

Department of Agronomy and Horticulture: Faculty Publications

Altering FA content in soybean [Glycine max (L.) Merr.] oil for improved functionality is a research goal of many soybean breeders. Several of the genes that alter palmitic, stearic, oleic, linoleic, and linolenic acids are modifier genes with small effects, causing these FA traits to act as quantitative traits. The objective of this study was to identify modifier FA quantitative trait loci (QTL) in soybean. A recombinant inbred line population was created from two prominent ancestors of currently avail-able U.S. cultivars (Essex and Williams) and grown in five environments. One hundred simple se-quence repeat markers spaced throughout the genome …

Elemental Uptake In Relation To Root Characteristics Of Tall Fescue, Glenn E. Shewmaker, Douglas A. Johnson, Henry F. Mayland, Scott A. Martin, Susie B. Hansen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

HiMag, an accession of tall fescue (Festuca arundinacea Schreb.), was selected for high magnesium (Mg) concentration in leaves to reduce grass tetany risk to ruminants. However, the mechanism for enhanced Mg uptake in HiMag leaves has not been determined. The objective was to investigate if increased Mg uptake in HiMag could be explained by differences in elemental distribution among plant parts, root characteristics, or organic acid concentrations compared to its parental cultivars, ‘‘Kentucky 31’’ (KY31) and ‘‘Missouri 96’’ (MO96). The study was conducted on a surface-irrigated calcareous Portneuf silt loam (coarse-silty, mixed, mesic, Durinodic Xeric Haplocalcid). Vegetation and soil …

Betaine Improves Growth, But Does Not Induce Whole Body Or Hepatic Palmitate Oxidation In Swine (Sus Scrofa Domestica), Diane Wray-Cahen, Ignacio Fernández-Fígares, Erkki Virtanen, Norman C. Steele, Thomas J. Caperna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dietary betaine may reduce carcass fat in growing pigs. We explored the effects of betaine on short-term growth and in vivo and in vitro fatty acid oxidation. Pigs were housed in metabolism crates and fed diets containing either 0% (control), 0.125% or 0.5% betaine at 80% of ad libitum energy intake. Fatty acid oxidation was measured during intravenous infusions of 1-¹³C-palmitate and in hepatocytes incubated in the presence or absence of betaine and carnitine. CO₂ and palmitate isotopic enrichments were determined by mass spectrometry. Pigs consuming 0.125% and 0.5% betaine for at least 9 days had growth …

Case Study: Effects Of Delaying Implant And Programmed Rate Of Gain On Performance And Carcass Characteristics Of Yearling Beef Steers, G. Scaglia, L. W. Greene, F. T. Mccollum, N. A. Cole, T. H. Montgomery

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ninety-six steers (average BW = 335 ± 36 kg) were allotted in a completely randomized design with a 2 × 2 factorial arrangement of treatments. Factors were 1) implant on d 1 or no implant and 2) ad libitum access to feed on d 1 or programmed-fed for a target BW gain of 1.4 kg/d during the first 62 d of the feeding period. On d 63, all steers were implanted with Revalor-S^® (Hoechst Roussel Vet, Overland Park, KS) and provided ad libitum access to feed until harvest. From d 63 to 116, ADG and gain efficiency (g gain/kg …

On-Station Production Of Native Speargrass Hay In Western Australia, G L. Krebs, Robert R. Rouda, S P. Van Wyngaarden

Bulletins 4000 -

This report documents the practical and economic feasibility of on-station harvesting and production of speargrass hay and haylage and its subsequent feeding to cattle and sheep in the extensive regions of south-eastern Western Australia. Four hundred and ten bales weighing an average 275 kg were produced from a total harvested area of 40.6 ha in the vicinity of Duck Dam on Kanandah Station. In the lead up to harvesting, seasonal conditions were among the best on record. Half of the bales produced were further processed into haylage. Two feeding trials, involving wethers at Arubiddy and steers at Kanandah, were undertaken …

B.R. Wells Rice Research Studies 2002, R. J. Norman, J.-F. Meullenet

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producing state in the U .S .. representing almost 46% of the total U .S. production and almost 47% of the total acres planted to rice . Rice cultural practices vary across the state and across the U.S. However, due to changing political, environmental, and economic times, the practices arc dynamic. This survey was initiated in 2002 to monitor how the changing times reflect the changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county Extension agents in each of the counties in Arkansas that produce rice. Questions …

Information On Pastoral Businesses In The Rangelands Of Western Australia, Department Of Agriculture, Western Australia

Farm Systems and Economics published reports

Edited by William Dalton and Francis Bright

The rangelands make up 87 per cent of Western Australia's landmass and include all but the south-west of the State. Livestock grazing on pastoral leasehold is the dominant commercial land use across 42 per cent (910,000 km*) of the WA rangelands and unallocated Crown lands comprise another 37 per cent.

The rangelands:

• Produce most of the State's mineral and energy wealth.

• Support important segments of the livestock industry.

• Are of special significance to the Aboriginal population.

• Offer major tourism potential.

• Contain essential elements of the State's biological diversity.

• Offer opportunities to produce horticultural …

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Sustainability Issues For Agriculture In Western Australia, Anne Bennett, Ross Kingwell, Ross George

Agriculture reports

This paper describes briefly the main challenges to the sustainability of agriculture in Western Australia and outlines some means of responding the the challenges: market-based approaches; agricultural and environmental research and development; accelerated adjustment; and global and national policy initiatives. Challenges include: land resource quality (salinity, soil acidity), biodiversity, greenhouse gas emissions, climate change, water availability, food safety, and pests and diseases.

Agroecosystems Analysis From The Grass Roots: A Multidimensional Experiential Learning Course, Mary Wiedenhoeft, Steve Simmons, Ricardo Salvador, Gina Mcandrews, Charles A. Francis, James W. King, David Hole

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

An intensive, experiential travel course in Agroecosystems Analysis was conducted in Iowa, Minnesota, and Nebraska (United States) during summers of 1998 and 1999. The intended student audience was advanced undergraduate and beginning graduate students. Pretravel readings and a week-long series of farm visits, which consisted of in-depth interviews with the farmers and their families, prepared student teams to analyze and evaluate the production, economic, environmental, and social sustainability of 10 farms. Students shared their analyses both orally and in written reports. Based on a multifaceted student evaluation process, we found that participants were highly motivated, strongly engaged with the course …

Mapping The Fas Locus Controlling Stearic Acid Content In Soybean, M. M. Spencer, V. R. Pantalone, E. J. Meyer, D. Landau-Ellis, D. L. Hyten Jr.

Department of Agronomy and Horticulture: Faculty Publications

Increasing the stearic acid content to improve soybean [Glycine max (L) Merr] oil quality is a desirable breeding objective for food-processing applications. Although a saturated fatty acid, stearic acid has been shown to reduce total levels of blood cholesterol and offers the potential for the production of solid fat products (such as margarine) without hydrogenation. This would result in the reduction of the level of trans fat in food products and alleviate some current health concerns. A segregating F₂ population was developed from the cross between Dare, a normal stearic acid content cultivar, and FAM94-41, a high stearic …

Intsormil 2003 Annual Report, John M. Yohe, Thomas Crawford Jr., Kimberly Christiansen, Joan Frederick

INTSORMIL Impacts and Bulletins

From 1980 to 1999, according to the Food and Agriculture Organization of the United Nations (FAO), the number of food-insecure people in developing countries fell from 920 million to about 800 million, yet in 2003, the International Food Policy Research Institute declared that "without significant changes in policies, public investments, and institutions, we simply will not achieve the 1996 World Food Summit goal-reaffirmed at the 2000 Millennium Summit and again last year at the World Food Summit: five years later of reducing the number of our fellow human beings who are food insecure by at least half by no later …

Fiber Quality Response Of Pima Cotton To Nitrogen And Phosphorus Deficiency, Haile Tewolde, C. J. Fernandez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Imposing a moderate level of nutrient deficiency may be an effective management strategy to limit vegetative growth and enhance maturity of Pima cotton (Gossypium barbadense L.). Whether such deficiency affects fiber quality of American Pima cotton, however, is not well known. A field study was conducted in 1991 and 1992 to determine the fiber quality responses of Pima cotton to nitrogen and phosphorus fertilization ranging between deficient and excess. Pima cotton cv. ‘‘S-7’’ was treated with nitrogen rates ranging between 0 and 269 kg ha^-1 in a factorial combination with phosphorus rates ranging between 0 and 44 kg …

Forage Yield And Economic Losses Associated With The Brown-Midrib Trait In Sudangrass, M. D. Casler, Jeffrey F. Pedersen, D. J. Undersander

Department of Agronomy and Horticulture: Faculty Publications

Brown-midrib genes increase digestibility due to reduced lignification in sudangrass, Sorghum bicolor subsp. drummondii (Nees ex Steud.) de Wet & Harlan. Brown-midrib lines are known to be low in forage yield potential, but this reduction in forage yield has not been previously quantified. The objectives of this study were to quantify the increase in forage quality and decrease in forage yield and to provide an economic assessment of this dichotomy. Piper and Greenleaf (normal leaves) were compared with their brown-midrib counterparts and four highly selected brown-midrib (FG) lines at two locations for 2 yr. Brown-midrib lines averaged 9.0% lower in …