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The Potential For Crop-To-Wild Gene Flow In Sorghum In Ethiopia And Niger: A Geographic Survey, Tesfaye Tesso, Issoufou Kapran, Cecile Grenier, Allison Snow, Patricia Sweeney, Jeffrey F. Pedersen, David B. Marx, Gurling Bothma, Gebisa Ejeta

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Information about the potential for crop–wild hybridization is needed to understand how crop genes, including transgenes, affect the population genetics and ecology of sexually compatible relatives. Transgenic sorghum is under development for use by traditional farmers in Africa, the center of origin for sorghum [Sorghum bicolor (L.) Moench], but systematic surveys of the current extent of contact with wild and weedy relatives are lacking. We studied wild and weedy sorghums that are interfertile with the crop and constitute a crop–wild–weed complex. The survey was conducted in 2005 in areas of traditional sorghum cultivation in three regions of Ethiopia and …

B.R. Wells Rice Research Studies 2007, R. J. Norman, J. F. Meullenet, K. A.K. Moldenhauer

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

In Vitro And In Vivo Techniques For Screening New Natural Product-Based Fungicides For Control Of Strawberry Anthracnose, Maritza Abril

Dissertations

Seven plant pathogenic fungi (Botrytis cinerea, Colletotrichum acutatum, C. fragariae, C. gloeosporioides, Fusarium oxysporum, Phomopsis obscurans, and P. viticola) valuable in screening fungicide efficacy were tested. Optimal and reproducible conditions for germination of these selected fungi were established by incorporating Roswell Park Memorial Institute 1640 (RPMI) as a medium of known composition and washing conidia to remove innate germination inhibitors. This step reduced average fungal germination times between 3.5 h and 21.2 h. The natural product-based fungicide, sampangine, seven sampangine analogs (4-bromo-sampangine, 4-methoxysampangine, benzo[4,5]sampangine, liriodenine Mel AMC-XIII-103, onychine, cryptolepine, and liriodenine CDH-II-37), plus seven conventional fungicides (benomyl, captan, cyprodinil, fenbuconazole, …

Proceedings Of The 35th Annual Meeting, Southern Soybean Disease Workers (March 12-13, 2008, Pensacola, Florida), Allen Wrather, Daniel H. Poston, Clayton A. Hollier, Jason Bond, Steve R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern United States Soybean Disease Loss Estimate for 2007. Compiled by SR Koenning

Contributed papers (Clayton Hollier, moderator)

Effects of Row Spacing and Leaf Wetness on the Temporal and Spatial Spread of Soybean Rust within Soybean Canopies. DF Narváez, JJ Marois, DL Wright, and S Isard

Effects of Potassium, Chloride, and Minor Elements Nutrition on Asian Soybean Rust. RW Schneider, EP Mumma, CL Clark, and CG Giles

The Impact of Selected Fungicide Treatments on Disease Progress of Asian Soybean Rust and Other Diseases of Soybean. GB Padgett, MA Purvis, A Hogan, and S Martin

Soybean Sudden Death Syndrome Variety …

Opportunities And Roadblocks In Utilizing Forages And Small Grains For Liquid Fuels, Gautam Sarath, Robert B. Mitchell, S. E. Sattler, Deanna L. Funnell, Jeffrey F. Pedersen, Robert A. Graybosch, Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This review focuses on the potential advantages and disadvantages of forages such as switchgrass (Panicum virgatum), and two small grains: sorghum (Sorghum bicolor), and wheat (Triticum aesitvum), as feedstocks for biofuels. It highlights the synergy provided by applying what is known from forage digestibility and wheat and sorghum starch properties studies to the biofuels sector. Opportunities therefore, exist to improve biofuel qualities in these crops via genetics and agronomics. In contrast to cereal crops, switchgrass still retains tremendous exploitable genetic diversity, and can be specifically improved to Wt a particular agronomic, management, and conversion …

Small Pasture Management Guide, James Barnhill, Scott Mckendrick

All Archived Publications

As more people select rural settings for their homes, traditional farms are being divided into small acreage home lots. Many small acreage owners would like to have lush green pastures where they can raise horses, cattle, or sheep. The purpose of this booklet is to provide the educational guidance these owners need to successfully establish and maintain a healthy grazing system.

Impact Of Solar Ultraviolet-B On The Proteome In Soybean Lines Differing In Flavonoid Contents, Chenping Xu, Joe H. Sullivan, Wesley M. Garrett, Thomas J. Caperna, Savithiry Natarajan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to systematically investigate the impact of solar ultraviolet- B (UV-B) radiation on the soybean leaf proteome. In order to investigate the protective role of flavonoids against UV-B, two isolines of the Clark cultivar (the standard line with moderate levels of flavonoids and the magenta line with reduced flavonoids) were grown in the field with or without natural levels of UV-B. The 12-day-old first trifoliates were harvested for proteomic analysis. More than 300 protein spots were reproducibly resolved and detected on each gel. Statistical analysis showed that 67 protein spots were significantly (P < 0.05) affected by solar UV-B. Many more spots were altered by UV-B in the magenta line than in the standard line. Another 12 protein spots were not altered by UV-B but showed significantly (P < 0.05) different accumulations between the two lines, and for most spots the line-specific differences were also observed under UV-B exclusion. Most of the differentially accumulated spots were identified by mass spectrometry. The proteins were quite diverse, and were involved in metabolism, energy, protein destination/storage, protein synthesis, disease/defense, transcription, and secondary metabolism. The results suggest that high levels of flavonoids lead to a reduction in UV-B sensitivity at the proteomic level.

Biotinyl-Methyl 4-(Amidomethyl)Benzoate Is A Competitive Inhibitor Of Human Biotinidase, Keyna Kobza, Kittichai Chaiseeda, Gautam Sarath, James M. Takacs, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Posttranslational modification of histones by biotinylation can be catalyzed by both biotinidase (BTD) and holocarboxylase synthetase. Biotinylation of histones is an important epigenetic mechanism to regulate gene expression, DNA repair, and chromatin remodeling. The role of BTD in histone biotinylation is somewhat ambiguous, given that BTD also catalyzes removal of the biotin tag from histones. Here, we sought to develop BTD inhibitors for future studies of the role of BTD in altering chromatin structure. We adopted an existing colorimetric BTD assay for use in a novel 96-well plate format to permit high-throughput screening of potential inhibitors. Biotin analogs were chemically …

Chapter 19. Remediation Of Drinking Water For Rural Populations, W. J. Hunter

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrate is the most common nitrogen contaminant in raw water supplies. In rural areas agricultural activities that involve the use of fertilizers and animal manures are major sources of nitrate contamination. Several processes are currently available that can effectively remove nitrate from raw water. Systems that are suitable for small rural communities include reverse osmosis, ion exchange, and electrodialysis. However, all of these systems move nitrate from raw water and concentrate it in a reject water or brine. Disposal of the reject water can be a major expense as well as an environmental issue. Several emerging systems are under development …

Auditing And Assessing Air Quality In Concentrated Feeding Operations, N. A. Cole, R. Todd, B. Auvermann, D. B. Parker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The potential adverse effects of concentrated animal feeding operations (CAFO) on the environment are a growing concern. The air quality issues of most concern to CAFO vary but generally include ammonia, hydrogen sulfide, particulate matter, volatile organic compounds, greenhouse gases, and odors. Air pollutants may be regulated by federal and state laws or by nuisance complaints. The United States Environmental Protection Agency, and poultry, swine, and dairy industries recently agreed to the National Air Emissions Monitoring System to fund research on atmospheric emissions from production farms in the United States. Air quality regulations may be based on actual emissions, atmospheric …

Evaluation Of Beef Cattle Operations Utilizing Different Seasons Of Calving, Weaning Strategies, Postweaning Management, And Retained Ownership, R. E. Kruse, M. W. Tess, E. E. Grings, R. E. Short, R. K. Heitschmidt, W. A. Phillips, H. S. Mayeux

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Data from a 3-yr study in Montana were utilized to evaluate impacts of season of calving, weaning strategy, and retained ownership of steer calves on enterprise profitability. Calving seasons were late winter (LW), early spring (ES), or late spring (LS). Each season had 2 weaning times: 190 (LW190, ES190) or 240 (LW240, ES240) d for LW and ES, and 140 (LS140) or 190 (LS190) d for LS. Backgrounding options included shipping steers to Oklahoma (OK1), or backgrounding in Montana to a constant age (MT2) or weight (MT3). Steers from OK1 and MT2 were finished in Oklahoma in confinement or via …

Ultraviolet Light Traps: Design Affects Attraction And Capture, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Traps that use ultraviolet light as an attractant for flies are widely used in urban situations. To determine the differences in trap efficacy from design and lighting, pairs of traps were compared under laboratory conditions. Comparisons were made between traps with open fronts and with traps with restricted open fronts, black light bulbs, and black light blue bulbs, and glue boards with and without z-9-tricosene pheromone. In a windowless laboratory, pairs of traps were placed approximately 90 cm above the floor and 3 m apart. Fifty mixed-sex, 3- to 5-day-old house flies (Musca domestica) were released and …

Effects Of Agricultural Conservation Practices On Fish And Wildlife Volume 2, Stuart R. Gagnon, Joseph R. Makuch, Cassandra Y. Harper

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This bibliography, in two volumes, is part of a multi-volume set developed by the Water Quality Information Center at the National Agricultural Library in support of the United States Department of Agriculture’s Conservation Effects Assessment Project (CEAP). The bibliography is a guide to recent scientific literature covering effects of agricultural conservation practices on fish and wildlife. The citations listed here provide information on how conservation programs and practices designed to improve fish and wildlife habitat, as well as those intended for other purposes, e.g., water quality improvement, affect various aquatic and terrestrial fauna.

Characterization Of Biological Types Of Cattle: Indicator Traits Offertility In Beef Cows, R. A. Cushman, M. F. Allan, L. A. Kuehn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Genetic diversity among breeds of cattle allows producers to select animals for specific environments or market conditions. Reproductive efficiency is a multi-component trait that is largely influenced by environmental influences such as health and nutritional status; however, there are clearly genetic components to reproductive efficiency, and breed differences in a number of indicator traits associated with fertility and cow productivity have been identified. Historical indicators of fertility include scrotal circumference, age at puberty, and postpartum interval. Both age at puberty and postpartum interval are laborious traits to collect in heifers and cows because they require many days of detection of …

Chapter 2. Transformation And Transport Processes Of Nitrogen In Agricultural Systems, Ronald Follett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This chapter discusses the transformation and transport processes of nitrogen (N) in agricultural systems and provides information on overall reservoir sizes for N. Nitrogen is ubiquitous in the environment and is required for the survival of all living things. It is also one of the most important essential nutrients and is central to the production of all crop plants. The most abundant form of N in the environment is elemental dinitrogen (N₂) gas that accounts for 78% of the atmosphere. N₂ gas is inert and is not directly available for plant uptake and metabolism. The atmospheric reservoir …

Auditing And Assessing Air Quality In Concentrated Feeding Operations, N. A. Cole, R. Todd, B. Auvermann, D. B. Parker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The potential adverse effects of concentrated animal feeding operations (CAFO) on the environment are a growing concern. The air quality issues of most concern to CAFO vary but generally include ammonia, hydrogen sulfide, particulate matter, volatile organic compounds, greenhouse gases, and odors. Air pollutants may be regulated by federal and state laws or by nuisance complaints. The United States Environmental Protection Agency, and poultry, swine, and dairy industries recently agreed to the National Air Emissions Monitoring System to fund research on atmospheric emissions from production farms in the United States. Air quality regulations may be based on actual emissions, atmospheric …

Nitrogen Capture By Grapevine Roots And Arbuscular Mycorrhizal Fungi From Legume Cover-Crop Residues Under Low Rates Of Mineral Fertilization, Xiaomei Cheng, Amy Euliss, Kendra Baumgartner

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The influence of mineral fertilization on root uptake and arbuscular mycorrhizal fungi-mediated ¹⁵N capture from labeled legume (Medicago polymorpha) residue was examined in winegrapes (Vitis vinifera) in the greenhouse, to evaluate compatibility of fertilization with incorporation of cover-crop residue in winegrape production. Plants grown in marginal vineyard soil were either fertilized with 0.25× Hoagland’s solution or not. This low fertilization rate represents the deficit management approach typical of winegrape production. Access to residue in a separate compartment was controlled to allow mycorrhizal roots (roots + hyphae), hyphae (hyphae-intact), or neither (hyphae-rotated) to proliferate in the …

Endocrine Responses Of Fast- And Slow-Growing Families Of Channel Catfish, Brian C. Peterson, Brian C. Small, Geoffrey C. Waldbieser, Brian G. Bosworth

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Sixty-eight families of USDA303 channel catfish Ictalurus punctatus were evaluated for growth performance for 30 d. The fastest- and slowest-growing catfish families were further evaluated to examine the hypothesis that genes or gene products associated with the growth regulatory and stress axes can be used to describe differences in growth performance. Research examined mRNA levels of genes involved in the growth hormone–insulin-like growth factor (GH–IGF) network in fast- (family A) and slow-growing (family H) USDA303 catfish. Fish (59.0 ± 2.4 g) were fed for 7 weeks, weighed, and had tissues for RNA extraction. The remaining fish were subjected to an …

A Gel-Based Reference Map Of The Porcine Hepatocyte Proteome, Thomas J. Caperna, Amy E. Shannon, Wesley M. Garrett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The overall goal of our research is to characterize and identify gene expression profiles of porcine hepatic cells. In this study, we have prepared two-dimensional electrophoresis maps of cytosol and membrane fractions from freshly prepared hepatocytes which were pooled from three crossbred pigs (35–69 kg). Following isoelectric focusing with three pH range immobilized pH gradient strips (pH 3–6, 5–8 and 7–10) and staining the second dimension gels with colloidal Coomassie blue, 728 protein spots were picked and digested with trypsin. Extracted tryptic peptides were initially subjected to matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI–TOF-MS) analysis for identification of proteins …

Hessian Fly, Jeffrey J. Stuart, Ming-Shun Chen, Marion O. Harris

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Hessian fly (Mayetiola destructor) is a gall-forming insect (Harris et al. 2006) with several attributes that make it suitable for genetic analysis: a short life cycle (30 days), a small genome (158 Mb) (Johnston et al. 2004), and polytene chromosomes. The reproductive biology and behavior of the insect also make it an attractive model (Harris and Rose 1989,1990; Bergh et al. 1990; Kanno and Harris 2000; Morris et al. 2000; Harris et al. 2001). Compared to most plant-feeding insects, Hessian flies can be reared in a small space. Unlike gall-forming aphid species, the Hessian fly is always …

Pastoral Profits Guide, A Paddock Guide To Achieving Sustainable Livestock Productivity, Mark Alchin, Jim Addison, Valarie Shrubb, Zac Cockerill, Matthew Young, Tim Johnson, Greg Brennan

All other publications

Most pastoralists appreciate the need to deliver an economic, environmental and social 'profit' from their business. Achieving these profits can be a real challenge given the comparatively low productive potential and the substantial seasonal variation in the WA Southern Rangelands region. This guide was written to assist pastoralists to meet their specific livestock production, financial and range condition objectives through the better alignment of feed demand (stocking rate) to feed supply (carrying capacity). We provide nine basic steps that simplify the process of managing the feed supply, equipping pastoralists with the tools to make· decisions and to critically assess the …

Effects Of Atmospheric Co2 Enrichment On Crop Nutrient Dynamics Under No-Till Conditions, Stephen A. Prior, G. Brett Runion, Hugo H. Rogers, H. Allen Torbert

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Increasing atmospheric CO₂ concentration could increase crop productivity and alter crop nutrient dynamics. This study was conducted (3 yrs) with two crops ([Glycine max (L.) Merr.] and grain sorghum [Sorghum bicolor (L.) Moench.]) grown under two CO₂ levels (ambient and twice ambient) using open top field chambers on a Blanton loamy sand under no-tillage. Macronutrient and micronutrient concentrations and contents were determined for grain, stover, and roots. Although elevated CO₂ tended to reduce nutrient concentrations, high CO₂ consistently increased nutrient content especially in grain tissue; this response pattern was more notable with macronutrients. The …

Social And Economic Aspects Of Areawide Pest Management, Sean P. Keenan, Paul A. Burgener

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This chapter explores demonstration elements from the United States Department of Agriculture, Agricultural Research Service's demonstration programme for cereal aphid AWPM. We explore elements of the demonstration programme as agricultural innovations. We discuss potential adoption of these elements by wheat producers on the Great Plains and implications of the programme outcomes for other wheat-growing regions of the world. We begin with a history of research on the adoption of agricultural innovations, which provides the context for evaluating the cereal aphid programme from the perspective of farmer adoption. Chapter 19, this volume, by Kristopher Giles et al. provides a summary and …

Improvement Of Perennial Forage Species As Feedstock For Bioenergy, William Anderson, Michael Casler, Brian S. Baldwin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Both native and non-native forage species other than switchgrass are less commonly considered as potential lignocellulosic feedstocks for bioenergy in the United States. These species hold potential as bioenergy feedstocks because of the experience and infrastructure that is already in place for management and harvest, and in certain areas of the country they have greater yield potential than switchgrass or other feedstocks. The forage grasses consist of temperate cool-season (most commonly C3) grasses as well as tropical or sub-tropical warm-season (C4) grasses. Some legume species may also play a significant role in supplying useful bioenergy feedstocks. Most have been researched …

Chapter 17. Nitrogen Management Modeling Techniques: Assessing Cropping Systems/Landscape Combinations, J. A. Delgado, M. J. Shaffer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen use efficiency (NUE) in production agriculture is often low, which results in losses of excess N to groundwater as NO₃-N, to gaseous emissions of NH₃ and N₂O, and to N losses in surface runoff and erosion. Best management practices (BMPs) are needed to improve efficiency levels while maintaining proper nutrition for crops. Field studies designed to investigate potential BMPs are both time consuming and costly, and cannot cover all scenarios. Application of simulation models with N cycling components in conjunction with associated field investigations offers methodology that can help identify BMPs that show promise …

Soil Properties And Macro Cations Status Impacted By Long-Term Applied Poultry Litter, Zhongqi He, Irenus A. Tazisong, Zachary N. Senwo, Donglin Zhang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Most ethnic populations worldwide consume poultry products. Whereas poultry litter (PL) is a traditionally inexpensive and effective fertilizer to improve soil quality and agricultural productivity, overapplication to soils has raised concerns because excess nutrients in runoff could accelerate the eutrophication of fresh bodies of water. A long-term field experiment of land application of PL to soils used for pasture growth has been maintained for nearly two decades in the Sand Mountain region of north Alabama, USA. In this work, several soil parameters impacted by the long-term applied litter were characterized. The findings clearly support previous general observations that long-term applied …

Winter Cover Crops And Vinegar For Early-Season Weed Control In Sustainable Cotton, P. J. Moran, S. M. Greenberg

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Weeds may be suppressed by winter cover crops and the use of organic herbicides such as vinegar. Black oat (Avena strigosa) and hairy vetch (Vicia villosa) winter cover crops were planted for 2 years as part of a sustainable production system for cotton in the Lower Rio Grande Valley of Texas, and were till-killed each spring prior to cotton planting. Palmer amaranth (Amaranthus palmeri), common purslane (Portulaca oleracea), and sunflower (Helianthus annuus) were frequently-encountered winter and spring weeds. Both cover crops controlled winter weeds as well as did winter tillage …

Asas Centennial Paper: Net Energy Systems For Beef Cattle – Cattles, Application, And Future Models, Calvin Ferrell, J. W. Oltjen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Development of nutritional energetics can be traced to the 1400s. Lavoisier established relationships among O₂ use, CO₂ production and heat production in the late 1700s, and the laws of thermodynamics and law of Hess were discovered during the 1840s. Those discoveries established the fundamental bases for nutritional energetics and enabled the fundamental entity ME = retained energy + heat energy to be established. Objectives became: 1) to establish relationships between gas exchange and heat energy, 2) to devise bases for evaluation of foods that could be related to energy expenditures, and 3) to establish causes of energy expenditures. …

Chapter 4. Relationship Of Environmental Nitrogen Metabolism To Human Health, J. R. Follett, R. F. Follett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The need for humans to produce and consume food and other agricultural products is increasing. This need is directly related to increasing world populations, demands for goods and services, and expectations. Nitrogen (N) is contained in all of the amino acids and proteins in the foods consumed by humans. The use of N to produce food and other products is generally increasing as human needs increase. Proteins are an essential component of the human diet because, unlike plants, humans are unable to utilize more simple forms of N and rely on food sources for protein which can then be digested …

Effect Of Sample Processing Procedures On Measurement Of Starch In Corn Silage And Corn Grain, M. B. Hall, D. R. Mertens

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Methods for processing feedstuffs before analysis can affect analytical results. Effects of drying temperature (corn silage), preservation method (corn grain), and grinding method (corn silage and grain) on starch analysis values were evaluated. Corn silage samples dried at 55 or 105°C and grain samples dried at 55°C were ground to pass the 1-mm screen of an abrasion mill or cutting mill and analyzed for free glucose and starch corrected for free glucose. Starch analyses were performed in triplicate to assess the effect of treatment on precision of starch determination. Drying at 105°C decreased free glucose and tended to decrease starch …