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Articles 1 - 4 of 4
Full-Text Articles in Social and Behavioral Sciences
Development Of Non-Antibiotic-Resistant, Chromosomally Based, Constitutive And Inducible Expression Systems For Aroa-Attenuated Salmonella Enterica Serovar Typhimurium, Jake Matic, Tamsin D. Terry, David Van Bockel, Tracy A. Maddocks, David Tinworth, Michael Jennings, Steven P Djordjevic, Mark J. Walker
Development Of Non-Antibiotic-Resistant, Chromosomally Based, Constitutive And Inducible Expression Systems For Aroa-Attenuated Salmonella Enterica Serovar Typhimurium, Jake Matic, Tamsin D. Terry, David Van Bockel, Tracy A. Maddocks, David Tinworth, Michael Jennings, Steven P Djordjevic, Mark J. Walker
Faculty of Science - Papers (Archive)
Live-vaccine delivery systems expressing two model antigens from Mycoplasma hyopneumoniae, F2(P97) (Adh) and NrdF, were constructed using Salmonella enterica serovar Typhimurium aroA (STM-1), and immunogenicity in mice was evaluated. Recombinant plasmid-based expression (PBE) and chromosomally based expression (CBE) systems were constructed. The PBE system was formed by cloning both antigen genes into pJLA507 to create an operon downstream of temperature-inducible promoters. Constitutive CBE was achieved using a promoter-trapping technique whereby the promoterless operon was stably integrated into the chromosome of STM-1, and the expression of antigens was assessed. The chromosomal position of the operon was mapped in four clones. Inducible …
Increased Mortality Of Naive Varanid Lizards After The Invasion Of Non-Native Cane Toads (Bufo Marinus), Thomas Madsen, Beata Ujvari
Increased Mortality Of Naive Varanid Lizards After The Invasion Of Non-Native Cane Toads (Bufo Marinus), Thomas Madsen, Beata Ujvari
Faculty of Science - Papers (Archive)
Exotic animal and plant species introduced into the Australian continent often imparted catastrophic effects on the indigenous fauna and flora. Proponents of biological control introduced the South American Cane Toad (Bufo marinus) into the sugar cane fields of Queensland in 1935. The Cane Toad is one of the most toxic bufonids, and when seized by naïve Australian predators, the toxin usually kills the attacker. One group of Australian squamate reptiles that are very susceptible to Cane Toad toxins is varanid lizards. Prior to Cane Toad invasion of our study area, the Adelaide River floodplain of the Northern Territory of Australia, …
A Fluorescence Quenching Assay To Discriminate Between Specific And Non-Specific Inhibitors Of Dengue Virus Protease, C Bodenreider, D Beer, T Keller, S Sonntag, D Wen, Li Yap, Y H Yau, S G Shocat, D Huang, T Zhou, A Caflisch, X C Su, Kiyoshi Ozawa, G Otting, S G. Vasudevan, J Lescar, S P. Lim
A Fluorescence Quenching Assay To Discriminate Between Specific And Non-Specific Inhibitors Of Dengue Virus Protease, C Bodenreider, D Beer, T Keller, S Sonntag, D Wen, Li Yap, Y H Yau, S G Shocat, D Huang, T Zhou, A Caflisch, X C Su, Kiyoshi Ozawa, G Otting, S G. Vasudevan, J Lescar, S P. Lim
Faculty of Science - Papers (Archive)
In drug discovery, the occurrence of false positives is a major hurdle in the search for lead compounds that can be developed into drugs. A small-molecular-weight compound that inhibits dengue virus protease at low micromolar levels was identified in a screening campaign. Binding to the enzyme was confirmed by isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR). However, a structure-activity relationship study that ensued did not yield more potent leads. To further characterize the parental compound and its analogues, we developed a high-speed, low-cost, quantitative fluorescence quenching assay. We observed that specific analogues quenched dengue protease fluorescence and showed …
Discovery Of A Non-Peptidic Inhibitor Of West Nile Virus Ns3 Protease By High-Throughput Docking, Dariusz Ekonomiuk, Xun-Cheng Su, Kiyoshi Ozawa, Chrisophe Bodenreider, Siew Pheng Lim, Zheng Yin, Thomas H. Keller, David Beer, Viral Patel, Gottfried Otting, Amedeo Calflisch, Danzhi Huang
Discovery Of A Non-Peptidic Inhibitor Of West Nile Virus Ns3 Protease By High-Throughput Docking, Dariusz Ekonomiuk, Xun-Cheng Su, Kiyoshi Ozawa, Chrisophe Bodenreider, Siew Pheng Lim, Zheng Yin, Thomas H. Keller, David Beer, Viral Patel, Gottfried Otting, Amedeo Calflisch, Danzhi Huang
Faculty of Science - Papers (Archive)
No abstract provided.