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Full-Text Articles in Social and Behavioral Sciences
Chemical Cross-Linking Of The Chloroplast Localized Small Heat-Shock Protein, Hsp21, And The Model Substrate Citrate Synthase, Emma Ahrman, W Lambert, Andrew Aquilina, C V Robinson, Cs Emanuelsson
Chemical Cross-Linking Of The Chloroplast Localized Small Heat-Shock Protein, Hsp21, And The Model Substrate Citrate Synthase, Emma Ahrman, W Lambert, Andrew Aquilina, C V Robinson, Cs Emanuelsson
J. A. Aquilina
The molecular mechanism whereby the small heat-shock protein (sHsp) chaperones interact with and prevent aggregation of other proteins is not fully understood. We have characterized the sHsp-substrate protein interaction at normal and increased temperatures utilizing a model substrate protein, citrate synthase (CS), widely used in chaperone assays, and a dodecameric plant sHsp, Hsp21, by chemical cross-linking with 3,3'-Dithiobis[sulfosuccinimidylpropionate] (DTSSP) and mass spectrometric peptide mapping. In the absence of CS, the cross-linker captured Hsp21 in dodecameric form, even at increased temperature (47 degrees C). In the presence of equimolar amounts of CS, no Hsp21 dodecamer was captured, indicating a substrate-induced Hsp21 …
Protein-Bound And Free Uv Filters In Cataract Lenses. The Concentration Of Uv Filters Is Much Lower Than In Normal Lenses, Roger Truscott, Andrew Aquilina, Anastasia Korlimbinis
Protein-Bound And Free Uv Filters In Cataract Lenses. The Concentration Of Uv Filters Is Much Lower Than In Normal Lenses, Roger Truscott, Andrew Aquilina, Anastasia Korlimbinis
J. A. Aquilina
In human cataract lenses the UV filters, 3-hydroxykynurenine glucoside (3OHKG) and kynurenine (Kyn) were found to be covalently bound to proteins and the levels in the nucleus were much higher than in the cortex. The levels of the bound UV filters in cataract nuclei were much lower than those in age-matched normal lenses. 3-Hydroxykynurenine could not be detected in cataract lenses. As with normal lenses, protein-bound 3OHKG in cataract lenses was found at the highest levels followed by Kyn. Free UV filter concentrations were also markedly reduced in cataract lenses. This feature may well contribute to the lower protein-bound levels; …
Protein-Bound Uv Filters In Normal Human Lenses: The Concentration Of Bound Uv Filters Equals That Of Free Uv Filters In The Centre Of Older Lenses, Roger Truscott, Andrew Aquilina, Anastasia Korlimbinis
Protein-Bound Uv Filters In Normal Human Lenses: The Concentration Of Bound Uv Filters Equals That Of Free Uv Filters In The Centre Of Older Lenses, Roger Truscott, Andrew Aquilina, Anastasia Korlimbinis
J. A. Aquilina
No abstract provided.