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Full-Text Articles in Social and Behavioral Sciences
Molecular Epidemiology Of A Fast-Food Restaurant-Associated Outbreak Of Escherichia Coli O157:H7 In Washington State, Linda M. Grimm, Marcia Goldoft, John Kobayashi, Jay H. Lewis, Dalia Alfi, Ann Marie Perdichizzi, Phillip I. Tarr, Jerry E. Ongerth, Steve L. Moseley, Mansour Samadpour
Molecular Epidemiology Of A Fast-Food Restaurant-Associated Outbreak Of Escherichia Coli O157:H7 In Washington State, Linda M. Grimm, Marcia Goldoft, John Kobayashi, Jay H. Lewis, Dalia Alfi, Ann Marie Perdichizzi, Phillip I. Tarr, Jerry E. Ongerth, Steve L. Moseley, Mansour Samadpour
Faculty of Engineering and Information Sciences - Papers: Part A
We studied the molecular epidemiology of the recent fast-food restaurant chain-associated Escherichia coli O157:H7 outbreak in Washington State. Genomic DNAs prepared from strains isolated from 433 patients were probed with radiolabelled Shiga-like toxin (SLT) I and SLT II genes and bacteriophage l DNA and were subsequently analyzed for their restriction fragment length polymorphism (RFLP) patterns. The SLT RFLP and l RFLP profiles of an E. coli O157:H7 strain isolated from the incriminated beef and prototype patient were compared with those of the patient isolates for determination of the concordance between patterns. Of the 377 patients with primary and secondary cases …
Occurrence Of Shiga-Like Toxin-Producing Escherichia Coli In Retail Fresh Seafood, Beef, Lamb, Pork, And Poultry From Grocery Stores In Seattle, Washington, Mansour Samadpour, Jerry Ongerth, J. Liston, Nhiem Tran, Dong Nguyen, Thomas S. Whittam, Richard Wilson, Phillip I. Tarr
Occurrence Of Shiga-Like Toxin-Producing Escherichia Coli In Retail Fresh Seafood, Beef, Lamb, Pork, And Poultry From Grocery Stores In Seattle, Washington, Mansour Samadpour, Jerry Ongerth, J. Liston, Nhiem Tran, Dong Nguyen, Thomas S. Whittam, Richard Wilson, Phillip I. Tarr
Faculty of Engineering and Information Sciences - Papers: Part A
Fresh meat, poultry, and seafood purchased from Seattle area grocery stores were investigated for the presence of Shiga-like toxin-producing Escherichia coli by using DNA probes for Shiga-like toxin (SLT) genes I and II. Of the 294 food samples tested, 17% had colonies with sequence homology to SLT I and/or SLT II genes.
Molecular Epidemiology Of Escherichia Coli 0157:H7 Strains By Bacteriophage A Restriction Fragment Length Polymorphism Analysis: Application To A Multistate Foodborne Outbreak And A Day-Care Center Cluster, Mansour Samadpour, Linda M. Grimm, B Desai, Dalia Alfi, Jerry E. Ongerth, Phillip I. Tarr
Molecular Epidemiology Of Escherichia Coli 0157:H7 Strains By Bacteriophage A Restriction Fragment Length Polymorphism Analysis: Application To A Multistate Foodborne Outbreak And A Day-Care Center Cluster, Mansour Samadpour, Linda M. Grimm, B Desai, Dalia Alfi, Jerry E. Ongerth, Phillip I. Tarr
Faculty of Engineering and Information Sciences - Papers: Part A
Genomic DNAs prepared from 168 isolates of Escherichia coli 0157:H7 were analyzed for restriction fragment length polymorphisms on Southern blots probed with bacteriophage A DNA. The isolates analyzed included strains from a recent large multistate outbreak of E. coli 0157:H7 infection associated with consumption of poorly cooked beef in restaurants, a day-care center cluster, and temporally and geographically unrelated isolates. E. coli 0157:H7 isolates recovered from the incriminated meat and from 61 (96.8%) of 63 patients from Washington and Nevada possessed identical A restriction fragment length patterns. The A restriction fragment length polymorphisms observed in 11 (91.7%) of 12 day-care …
Evaluation Of Dna Probes For Detection Of Shiga-Like-Toxin- Producing Escherichia Coli In Food And Calf Fecal Samples, Mansour Samadpour, John Liston, Jerry E. Ongerth, Phillip I. Tarr
Evaluation Of Dna Probes For Detection Of Shiga-Like-Toxin- Producing Escherichia Coli In Food And Calf Fecal Samples, Mansour Samadpour, John Liston, Jerry E. Ongerth, Phillip I. Tarr
Faculty of Engineering and Information Sciences - Papers: Part A
The use of DNA probes for Shiga-like toxin I (SLT-I) and SLT-II for detection of SLT-producing Escherichia coli (SLTEC) in foods and calf fecal samples was evaluated. Enrichment cultures were prepared from food or fecal samples. Colonies formed by plating of enrichment cultures were probed for SLTEC by colony hybridization. Alternatively, enrichment cultures were analyzed for SLTEC presence by dot blot. The lowest detected concentration of SLTEC in sample homogenates inoculated with E. coli 0157:H7 corresponded to 1.3 CFU/g of sample. Of the 44 food samples and 28 fecal samples from dairy calves tested by the colony hybridization method, 4 …