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Full-Text Articles in Social and Behavioral Sciences

Methodological Improvements In The Mrna Profiling Assays For Incorporation Into Dna Casework Workflows, Paris Volk May 2019

Methodological Improvements In The Mrna Profiling Assays For Incorporation Into Dna Casework Workflows, Paris Volk

Electronic Theses and Dissertations

Currently, DNA profiling is the gold standard to identify an individual. However, determining body fluid origin is important in criminal investigations, offering additional information surrounding the circumstances of a crime. However, crime labs can only definitively identify blood and semen and presumptively saliva using techniques that consume time and sample and do not simultaneously identify all forensically relevant body fluids. This causes many crime labs to want to bypass body fluid identification altogether. Therefore, advances into more definitive molecular-based body fluid methods are necessary. One such technique is mRNA profiling because it provides a highly sensitive and specific approach to …


Federal, State And Local Law Enforcement Agency Interoperability Capabilities And Cyber Vulnerabilities, Tyrone Trapnell May 2019

Federal, State And Local Law Enforcement Agency Interoperability Capabilities And Cyber Vulnerabilities, Tyrone Trapnell

Electronic Theses and Dissertations

The National Data Exchange (N-DEx) System is the central informational hub located at the Federal Bureau of Investigation (FBI). Its purpose is to provide network subscriptions to all Federal, state and local level law enforcement agencies while increasing information collaboration across all domains. The National Data Exchange users must satisfy the Advanced Permission Requirements, confirming the terms of N-DEx information use, and the Verification Requirement (verifying the completeness, timeliness, accuracy, and relevancy of N-DEx information) through coordination with the record-owning agency (Management, 2018). A network infection model is proposed to simulate the spread impact of various cyber-attacks within Federal, state …


Chemometric Applications In Fire Debris Analysis: Likelihood Ratios From Naive Bayes And Frequency Of Component And Pyrolysis Product Occurrence, Anuradha Akmeemana Jan 2019

Chemometric Applications In Fire Debris Analysis: Likelihood Ratios From Naive Bayes And Frequency Of Component And Pyrolysis Product Occurrence, Anuradha Akmeemana

Electronic Theses and Dissertations

One of the major challenges in fire investigation is the determination of the cause of fire. The fire can be accidental or intentional. The determination of ignitable liquid residue (ILR) from fire debris helps the process and this process is called fire debris analysis in forensic science. This is one of the most complex areas in the field of forensics because of the evaporation of the ILR from the debris and the interference of the substrate matrix with the ILR if present. In the present, the final decisions in fire debris analysis are based on categorical statements and it only …


The Evaluation Of Classifier Performance On The Forensic Analysis Of Fire Debris And The Expansion Of The Substrate Database, Alyssa Allen Jan 2019

The Evaluation Of Classifier Performance On The Forensic Analysis Of Fire Debris And The Expansion Of The Substrate Database, Alyssa Allen

Electronic Theses and Dissertations

The current protocols in fire debris analysis rely on ignitable liquid pattern recognition and the identification of target compounds. These practices allow fire debris analysts to determine whether a sample contains or is absent of ignitable liquid residue and to classify that type of ignitable liquid based upon subjective thresholds. A statistical approach using computationally mixed datasets was devised in this research to generate an objective approach to the classification of fire debris samples and to determine error rates. Quadratic, linear, and partial least squares linear discriminant analysis (QDA, LDA, and PLS-DA) models were developed and evaluated to determine the …


Assessment Of Information Security Culture In Higher Education, Henry Glaspie Jan 2018

Assessment Of Information Security Culture In Higher Education, Henry Glaspie

Electronic Theses and Dissertations

Information security programs are instituted by organizations to provide guidance to their users who handle their data and systems. The main goal of these programs is to protect the organization's information assets through the creation and cultivation of a positive information security culture within the organization. As the collection and use of data expands in all economic sectors, the threat of data breach due to human error increases. Employee's behavior towards information security is influenced by the organizations information security programs and the overall information security culture. This study examines the human factors of an information security program and their …


Qualitative And Quantitative Elemental Composition Analysis Of The Surrounding Pollen Coating Via Icp-Ms, Brandy Voyer Jan 2018

Qualitative And Quantitative Elemental Composition Analysis Of The Surrounding Pollen Coating Via Icp-Ms, Brandy Voyer

Electronic Theses and Dissertations

Pollen evidence has proven to be a powerful forensic tool to trace a suspect or item to a victim or a crime scene. This is possible because it is microscopic, abundant in nature, resistant to degradation and decay; it presents dispersal patterns that can be used to generate a 'fingerprint' within specific areas, and has illustrated a unique morphology that can be used to classify species. While the pollen grain morphology has been extensively used to characterize the specific species, not much has been investigated as pertains to the coating that surrounds the pollen grain aside from it being categorized …


Strategies For Enhanced Genetic Analysis Of Trace Dna From Touch Dna Evidence And Household Dust, Katherine Farash Jan 2015

Strategies For Enhanced Genetic Analysis Of Trace Dna From Touch Dna Evidence And Household Dust, Katherine Farash

Electronic Theses and Dissertations

In forensic casework it is often necessary to obtain genetic profiles from crime scene samples that contain increasingly smaller amounts of genetic material, called Low Template DNA (LTDNA). Two examples of LTDNA sources are touch DNA evidence and dust bunnies. Touch DNA refers to DNA that is left behind through casual contact of a donor with an object or another person. Touch DNA can be used to prove a suspect was present at a crime scene. Dust bunnies, or dust conglomerates, typically contain trapped shed skin cells of anyone in the vicinity along with fibers, dirt, hair, and other trace …


Quantitative Assessment Of The Effects Of Microbial Degradation Of A Simple Hydrocarbon Mixture, Jessica Kindell Jan 2015

Quantitative Assessment Of The Effects Of Microbial Degradation Of A Simple Hydrocarbon Mixture, Jessica Kindell

Electronic Theses and Dissertations

Ignitable liquids consist of either a single organic compound or a complex organic mixture. In regards to fire debris analysis, the analyst is responsible for determining if an ignitable liquid residue is present. However, when extracted from soil-containing fire debris evidence, chemical degradation from microorganisms is observed to result in the loss of compounds based on chemical structure. It can also happen when the evidence container is stored at room temperature before analysis. This can present a challenge to the fire debris analyst when identifying and classifying the ignitable liquid residue based on the criteria established by standard test methods. …


Providing Context To The Clues: Recovery And Reliability Of Location Data From Android Devices, Connie Bell Jan 2015

Providing Context To The Clues: Recovery And Reliability Of Location Data From Android Devices, Connie Bell

Electronic Theses and Dissertations

Mobile device data continues to increase in significance in both civil and criminal investigations. Location data is often of particular interest. To date, research has established that the devices are location aware, incorporate a variety of resources to obtain location information, and cache the information in various ways. However, a review of the existing research suggests varying degrees of reliability of any such recovered location data. In an effort to clarify the issue, this project offers case studies of multiple Android mobile devices utilized in controlled conditions with known settings and applications in documented locations. The study uses data recovered …


Development Of Micro Volume Dna And Rna Profiling Assays To Identify The Donor And Tissue Source Of Origin Of Trace Forensic Biological Evidence, Brittany Morgan Jan 2013

Development Of Micro Volume Dna And Rna Profiling Assays To Identify The Donor And Tissue Source Of Origin Of Trace Forensic Biological Evidence, Brittany Morgan

Electronic Theses and Dissertations

In forensic casework analysis it is necessary to obtain genetic profiles from increasingly smaller amounts of biological material left behind by perpetrators of crime. The ability to obtain profiles from trace biological evidence is demonstrated with so-called ‘touch DNA evidence’ which is perceived to be the result of DNA obtained from shed skin cells transferred from donor to an object or person during physical contact. However, the current method of recovery of trace DNA involves cotton swabs or adhesive tape to sample an area of interest. This "blindswabbing" approach may result in the recovery of biological material from different individuals …


Turtle Cam: Live Multimedia Interaction For Engaging Potential Visitor Population To Canaveral National Seashore, Brian Alfred Tortorelli Jan 2012

Turtle Cam: Live Multimedia Interaction For Engaging Potential Visitor Population To Canaveral National Seashore, Brian Alfred Tortorelli

Electronic Theses and Dissertations

This project expands the outreach of the Canaveral National Seashore to its visitors, potential visitors, and virtual visitors through its goals in conservancy and preservation of its natural resources. This paper is involved with the current iteration of a series of digital media projects, the Sea Turtle Nest Camera, also known as, Turtle Cam. It details how and why this project was designed to be an ongoing initiative to assist in those goals.


Hfs Plus File System Exposition And Forensics, Scott Ware Jan 2012

Hfs Plus File System Exposition And Forensics, Scott Ware

Electronic Theses and Dissertations

The Macintosh Hierarchical File System Plus, HFS +, or as it is commonly referred to as the Mac Operating System, OS, Extended, was introduced in 1998 with Mac OS X 8.1. HFS+ is an update to HFS, Mac OS Standard format that offers more efficient use of disk space, implements international friendly file names, future support for named forks, and facilitates booting on non-Mac OS operating systems through different partition schemes. The HFS+ file system is efficient, yet, complex. It makes use of B-trees to implement key data structures for maintaining meta-data about folders, files, and data. The implementation of …


The Implications Of Virtual Environments In Digital Forensic Investigations, Farrah M. Patterson Jan 2011

The Implications Of Virtual Environments In Digital Forensic Investigations, Farrah M. Patterson

Electronic Theses and Dissertations

This research paper discusses the role of virtual environments in digital forensic investigations. With virtual environments becoming more prevalent as an analysis tool in digital forensic investigations, it’s becoming more important for digital forensic investigators to understand the limitation and strengths of virtual machines. The study aims to expose limitations within commercial closed source virtual machines and open source virtual machines. The study provides a brief overview of history digital forensic investigations and virtual environments, and concludes with an experiment with four common open and closed source virtual machines; the effects of the virtual machines on the host machine as …


The Application Of Chemometrics To The Detection And Classification Of Ignitable Liquids In Fire Debris Using The Total Ion Spectrum, Jennifer N. Lewis Jan 2011

The Application Of Chemometrics To The Detection And Classification Of Ignitable Liquids In Fire Debris Using The Total Ion Spectrum, Jennifer N. Lewis

Electronic Theses and Dissertations

Current methods in ignitable liquid identification and classification from fire debris rely on pattern recognition of ignitable liquids in total ion chromatograms, extracted ion profiles, and target compound comparisons, as described in American Standards for Testing and Materials E1618-10. The total ion spectra method takes advantage of the reproducibility among sample spectra from the same American Society for Testing and Materials class. It is a method that is independent of the chromatographic conditions that affect retention times of target compounds, thus aiding in the use of computer-based library searching techniques. The total ion spectrum was obtained by summing the ion …


The Relative Recoverability Of Dna And Rna Profiles From Forensically Relevant Body Fluid Stains, Charly Parker Jan 2011

The Relative Recoverability Of Dna And Rna Profiles From Forensically Relevant Body Fluid Stains, Charly Parker

Electronic Theses and Dissertations

Biological material (fluids or tissues) whether from the victim or suspect is often collected as forensic evidence, and methods to obtain and analyze the DNA found in that material have been well established. The type of body fluid (i.e. blood, saliva, semen, vaginal secretions, and menstrual blood) from which the DNA originated is also of interest, and messenger RNA typing provides a specific and sensitive means of body fluid identification. In order for mRNA profiling to be utilized in routine forensic casework, RNA of sufficient quantity and quality must be obtained from biological fluid stains and the methods used for …


Black Writing Ink Analysis By Direct Infusion Electrospray Mass Spectroscopy, Christopher M. Moody Jan 2010

Black Writing Ink Analysis By Direct Infusion Electrospray Mass Spectroscopy, Christopher M. Moody

Electronic Theses and Dissertations

An optimized method of extraction, an instrumental analysis method and data analysis was proposed for black writing inks based on direct infusion electrospray-mass spectrometry (ESI-MS). The sampling and analysis method is both minimally destructive and able to assess differences in inks from a reference collection of thirty ballpoint, gel, and rollerball inks. The methanol extracts of ink on paper samples were analyzed with three direct infusion (ESI-MS) methods. Each method varied scan voltage negative and positive, ESI fragmentor applied voltage (+120V, +0V, and -120V), and mobile phase additive. Direct infusion ESI-MS analysis, followed by pair-wise comparisons of the observed ion …


Determining The Presence Of An Ignitable Liquid Residue In Fire Debris Samples Utilizing Target Factor Analysis, Kelly Mchugh Jan 2010

Determining The Presence Of An Ignitable Liquid Residue In Fire Debris Samples Utilizing Target Factor Analysis, Kelly Mchugh

Electronic Theses and Dissertations

Current fire debris analysis procedure involves using the chromatographic patterns of total ion chromatograms, extracted ion chromatograms, and target compound analysis to identify an ignitable liquid according to the American Society for Testing and Materials (ASTM) E 1618 standard method. Classifying the ignitable liquid is accomplished by a visual comparison of chromatographic data obtained from any extracted ignitable liquid residue in the debris to the chromatograms of ignitable liquids in a database, i.e. by visual pattern recognition. Pattern recognition proves time consuming and introduces potential for human error. One particularly difficult aspect of fire debris analysis is recognizing an ignitable …


Water And The Mountains: Maya Water Mangement At Caracol, Belize, James Crandall Jan 2009

Water And The Mountains: Maya Water Mangement At Caracol, Belize, James Crandall

Electronic Theses and Dissertations

Water management techniques in the Southern Maya Lowlands are both regionally diverse and site specific. This thesis examines the water management strategies of the Classic Period Maya at the site of Caracol, Belize. While it is likely that elites at Caracol controlled the redistribution of resources, i.e. craft and agricultural products, it is probable that the production of agricultural resources and the maintenance of water resource acquisition took place on a more local level. In order to test this hypothesis, a sample of five reservoirs were examined through original research -- and situated in conjunction with past settlement studies -- …


The Forensic Analysis Of Triacetone Triperoxide (Tatp) Precursors And Synthetic By-Products, Kimberly Painter Jan 2009

The Forensic Analysis Of Triacetone Triperoxide (Tatp) Precursors And Synthetic By-Products, Kimberly Painter

Electronic Theses and Dissertations

Triacetone Triperoxide (TATP) is a primary high explosive that can be synthesized using commercially available starting materials and has grown in use among terrorists over the past several years. Additives present in the precursors were investigated to see if they carry through the TATP synthesis and can be detected in the final product potentially aiding in the identification of the source. Additives identified in the acetones were also identified in pre-blast and in some post-blast samples. However, these additives are present in trace quantities relative to the TATP, which coupled with the volatility and short lifetimes of some of the …


Forensic Analysis Of Automobile Paints By Atomic And Molecular Spectroscopic Methods And Statistical Data Analyses, Erin Mcintee Jan 2008

Forensic Analysis Of Automobile Paints By Atomic And Molecular Spectroscopic Methods And Statistical Data Analyses, Erin Mcintee

Electronic Theses and Dissertations

The analysis of 110 automotive paint samples was conducted for the research presented here. Laser-induced breakdown spectroscopy (LIBS) was the central instrument utilized for analysis although scanning electron microscopy / energy dispersive x-ray spectroscopy (SEM/EDS) and Fourier transform infrared spectroscopy - attenuated total reflection (FTIR-ATR) analyses were also performed. Two separate methods of LIBS analysis of samples were used: a cross sectional analysis and a drill down analysis. SEM/EDS analysis focused on the cross section while FTIR-ATR analysis concentrated on the clearcoat layer. Several different data/statistical analyses were evaluated including principal components analysis (PCA), two tailed t-tests based on several …


The Identification Of Ignitable Liquids In The Presence Of Pyrolysis Products: Generation Of A Pyrolysis Product Database, Joseph Castelbuono Jan 2008

The Identification Of Ignitable Liquids In The Presence Of Pyrolysis Products: Generation Of A Pyrolysis Product Database, Joseph Castelbuono

Electronic Theses and Dissertations

The fire debris analyst is often faced with the complex problem of identifying ignitable liquid residues in the presence of products produced from pyrolysis and incomplete combustion of common building and furnishing materials. The purpose of this research is to investigate a modified destructive distillation methodology provided by the Florida Bureau of Forensic Fire and Explosive Analysis to produce interfering product chromatographic patterns similar to those observed in fire debris case work. The volatile products generated during heating of substrate materials are extracted from the fire debris by passive headspace adsorption and subsequently analyzed by GC-MS. Low density polyethylene (LDPE) …


Chromatographic And Mass Spectral Analyses Of Oligosaccharides And Indigo Dye Extracted From Cotton Textiles With Manova And Ano, Jessica Frisch Jan 2008

Chromatographic And Mass Spectral Analyses Of Oligosaccharides And Indigo Dye Extracted From Cotton Textiles With Manova And Ano, Jessica Frisch

Electronic Theses and Dissertations

Research was conducted on thirteen 100% cotton denim samples using an acid wash, established by Murray, to extract oligosaccharides from the cellulosic material. The oligosaccharide ion groups (+, +, and +) for molecules with degrees of polymerization between two and seven (DP2-DP7) were analyzed using liquid chromatography coupled to mass spectrometry with an electrospray ionization interface (LC-ESI-MS). The results were compared using the least-squares means in a Multivariate ANOVA (MANOVA) test followed by Univariate ANOVA and Tukey HSD tests and demonstrated that the method could correctly determine that two samples were statistically different 85.9% of the time when analyzing the …


Development And Forensic Application Of Dye Probe Fluorescence Resonance Energy Transfer For Improved Detection Of Changes In Dn, Micah Halpern Jan 2008

Development And Forensic Application Of Dye Probe Fluorescence Resonance Energy Transfer For Improved Detection Of Changes In Dn, Micah Halpern

Electronic Theses and Dissertations

Discovering, screening, and associating changes in DNA sequence are important to a broad range of disciplines and play a central role in Forensic Science. The typical types of changes include sequence variations [single nucleotide polymorphisms (SNP)] and length variations [short tandem repeats (STR)]. The steps for forensic DNA sample processing are similar for both types of changes but diverge at the point of detection. A number of approaches are being explored for SNP genotyping while STR analysis primarily consists of size-based analysis by capillary electrophoresis. Limitations exist for all current detection methods that pose significant impacts to forensic analysis. Bi-allelic …


Advances In Fire Debris Analysis, Mary Williams Jan 2007

Advances In Fire Debris Analysis, Mary Williams

Electronic Theses and Dissertations

Fire incidents are a major contributor to the number of deaths and property losses within the United States each year. Fire investigations determine the cause of the fire resulting in an assignment of responsibility. Current methods of fire debris analysis are reviewed including the preservation, extraction, detection and characterization of ignitable liquids from fire debris. Leak rates were calculated for the three most common types of fire debris evidence containers. The consequences of leaking containers on the recovery and characterization of ignitable liquids were demonstrated. The interactions of hydrocarbons with activated carbon during the extraction of ignitable liquids from the …


Maya Eclipses: Modern Data, The Triple Tritos And The Double Tzolkin, William Earl Beck Jan 2007

Maya Eclipses: Modern Data, The Triple Tritos And The Double Tzolkin, William Earl Beck

Electronic Theses and Dissertations

The Eclipse Table, on pages 51-58, of the Dresden Codex has long fascinated Maya scholars. Researchers use the mean-value method of 173.3 days to determine nodal passage that is the place where eclipses can occur. These studies rely on Oppolzer's Eclipse Canon and Schram's Moon Phase Tables to verify eclipse occurrences. The newer canons of Jean Meeus and Bao-Lin Liu use decimal accuracy. What would be the effect of modern astronomical data on the previous studies and the Maya Eclipse Table? The study utilizes a general view of eclipses that includes eclipses not visible to the Maya. Lunar eclipses are …


Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker Jan 2007

Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker

Electronic Theses and Dissertations

To date, the use of mitochondrial DNA in forensic analysis has relied on the presence of variations in the control region to differentiate between samples. One problem that this analysis has shown is the occurrence of common Haplogroup H haplotypes or identical sequences. Thus, there is a need to enhance the distinguishing power of this type of analysis. One option has been to investigate the mitochondrial coding region for polymorphisms that could differentiate between samples with identical control region haplotypes. The goal of this study has been to identify polymorphic coding region sites for development in a Pyrosequencing assay that …


Forensic Analysis Of C-4 And Commercial Blasting Agents For Possible Discrimination, Katie Steele Jan 2007

Forensic Analysis Of C-4 And Commercial Blasting Agents For Possible Discrimination, Katie Steele

Electronic Theses and Dissertations

The criminal use of explosives has increased in recent years. Political instability and the wide spread access to the internet, filled with "homemade recipes," are two conjectures for the increase. C-4 is a plastic bonded explosive (PBX) comprised of 91% of the high explosive RDX, 1.6% processing oils, 5.3% plasticizer, and 2.1% polyisobutylene (PIB). C-4 is most commonly used for military purposes, but also has found use in commercial industry as well. Current methods for the forensic analysis of C-4 are limited to identification of the explosive; however, recent publications have suggested the plausibility of discrimination between C-4 samples based …


Camera System Support For Highway Transportation Using Mobile Devices, Le Minh Jan 2004

Camera System Support For Highway Transportation Using Mobile Devices, Le Minh

Electronic Theses and Dissertations

With the very fast growing technology in wireless, advancement in hardware and the dramatically falling cost of mobile computing devices such as PDA, handheld device, People nowadays can have a personal device that fits in their hand but has computing power as a desktop did few years ago. The same device now is able to communicate over a wireless network and view office document at the same time. The combination of size, power and flexibility makes the personal devices increasingly appear in many aspects of life. In this proposal, we focus on a simple yet useful application of mobile devices …