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A Pilot Study Into The Effects Of X-Ray And Computed Tomography Exposure On The Amplification Of Dna From Bone, Britta M. Grieshaber, Daniel L. Osborne, Alison F. Doubleday, Frederika A. Kaestle

Department of Anthropology: Faculty Publications

This research investigates the intersection of radiography and aDNA, two commonly used methods in bioarchaeology. The goal of this project was to investigate the effects of radiation on the ability to amplify DNA from bone. Bones (n = 124) from domestic pig (Sus scrofa) feet were randomly sorted into a control group and four treatment groups: (1) single exposure X-ray; (2) single exposure CT; (3) multiple exposures X-ray; and (4) multiple exposures CT. Number of PCR cycles required to amplify DNA in 100 bp, 200 bp and 400 bp segments were used as a proxy for the …