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Characterization Of The Domain Of Fibronectin-Binding Protein Of I Of Streptococcus Pyogenes Responsible For Elicitation Of A Protective Immune Response, Kai Schulze, Eva Medina, Susanne R. Talay, Rebecca J. Towers, Gursharan S. Chhatwal, Carlos A. Guzman

Faculty of Science, Medicine and Health - Papers: part A

Fibronectin-binding protein I (SfbI) represents a major adhesin ofStreptococcus pyogenes. Mice were intranasally immunized with recombinant proteins spanning different portions of SfbI to identify the minimal fragment able to elicit a protective response against a lethal challenge with S. pyogenes. The strongest cellular responses and the highest levels of antigen-specific secretory immunoglobulin A (IgA) were detected in mice immunized with the fibronectin-binding region of SfbI. In contrast, animals vaccinated with a polypeptide spanning the aromatic and proline-rich regions showed the highest titers and fastest IgG response in serum. Vaccination with either SfbI without a membrane anchor and …