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Full-Text Articles in Social and Behavioral Sciences
Identification Of Human Plasma Proteins As Major Clients For The Extracellular Chaperone Clusterin, Amy R. Wyatt, Mark R. Wilson
Identification Of Human Plasma Proteins As Major Clients For The Extracellular Chaperone Clusterin, Amy R. Wyatt, Mark R. Wilson
Faculty of Science - Papers (Archive)
Clusterin (CLU) is an extracellular chaperone that is likely to play an important role in protein folding quality control. This study identified three deposition disease-associated proteins as major plasma clients for clusterin by studying CLU-client complexes formed in response to physiologically relevant stress (shear stress, similar to 36 dynes/cm(2) at 37 degrees C). Analysis of plasma samples by size exclusion chromatography indicated that (i) relative to control plasma, stressed plasma contained proportionally more soluble protein species of high molecular weight, and (ii) high molecular weight species were far more abundant when proteins purified by anti-CLU immunoaffinity chromatography from stressed plasma …
The Two-Faced Nature Of Small Heat Shock Proteins: Amyloid Assembly And The Inhibition Of Fibril Formation. Relevance To Disease States, Heath W. Ecroyd, S Meehan, John A. Carver
The Two-Faced Nature Of Small Heat Shock Proteins: Amyloid Assembly And The Inhibition Of Fibril Formation. Relevance To Disease States, Heath W. Ecroyd, S Meehan, John A. Carver
Faculty of Science - Papers (Archive)
The ability of small heat-shock proteins (sHsps) such as alphaB-crystallin to inhibit the amorphous (disordered) aggregation of varied target proteins in a chaperone-like manner has been well described. The mechanistic details of this action are not understood. Amyloid fibril formation is an alternative off-folding pathway that leads to highly ordered beta-sheet-containing aggregates. Amyloid fibril formation is associated with a broad range of protein conformational diseases such as Alzhiemer's, Parkinson's and Huntington's and sHsp expression is elevated in the protein deposits that are characteristic of these disease states. The ability of sHsps to prevent fibril formation has been less well characterised. …
What You Need To Know About Selenium, Terry Young, Keith Finley, William Adams, John Besser, William D. Hopkins, Dianne F. Jolley, Eugenia Mcnaughton, Theresa S. Presser, D. Patrick Shaw, Jason Unrine
What You Need To Know About Selenium, Terry Young, Keith Finley, William Adams, John Besser, William D. Hopkins, Dianne F. Jolley, Eugenia Mcnaughton, Theresa S. Presser, D. Patrick Shaw, Jason Unrine
Faculty of Science - Papers (Archive)
No abstract provided.
Toxicity To Melita Plumulosa From Intermittent And Continuous Exposures To Dissolved Copper, Brad M. Angel, Stuart L. Simpson, Dianne F. Jolley
Toxicity To Melita Plumulosa From Intermittent And Continuous Exposures To Dissolved Copper, Brad M. Angel, Stuart L. Simpson, Dianne F. Jolley
Faculty of Science - Papers (Archive)
The concentrations of metal contaminants often fluctuate in estuarine waters; yet we have limited knowledge about the effects of intermittent exposures on estuarine organisms. Using 10-d lethality bioassays with the epibenthic amphipod Melita plumulosa, different combinations of intermittent (pulsed) dissolved Cu exposure were investigated, varying Cu concentration, pulse duration, and time between pulses. Negligible organism mortality was observed immediately after single 12- to 62-h duration pulsed exposures of 100 to 900 mg/L dissolved Cu. However, delayed mortality was observed in the subsequent 96-h nonexposure period, after which negligible additional mortality occurred during the remainder of the 240-h tests. For multiple …
Synthesis Of Polyhydroxylated Pyrrolizidine And Indolizidine Compounds And Their Glycosidase Inhibitory Activities, Thunwadee Ritthiwigrom, Robert J. Nash, Stephen G. Pyne
Synthesis Of Polyhydroxylated Pyrrolizidine And Indolizidine Compounds And Their Glycosidase Inhibitory Activities, Thunwadee Ritthiwigrom, Robert J. Nash, Stephen G. Pyne
Faculty of Science - Papers (Archive)
The synthesis of the natural product 3-epi-casuarine and two tricyclic ether bridged analogues, plus 7-deoxy-3,6-diepi-casuarine, 7-epi-australine, 1-epi-castanospermine and 1,6-diepi-castanospermine is described. The glycosidase inhibitory activities of these compounds, along with that of uniflorine A and other polyhydroxylated pyrrolizidines and indolizidines that we have published before, are reported.
Synthesis Of 2′-Aminoalkyl-1-Benzylisoquinoline Derivatives And Medium Sized Ring Analogues With Mu Opiod Receptor Binding Activities, Uta Mbere-Nguyen, Alison T. Ung, Stephen G. Pyne
Synthesis Of 2′-Aminoalkyl-1-Benzylisoquinoline Derivatives And Medium Sized Ring Analogues With Mu Opiod Receptor Binding Activities, Uta Mbere-Nguyen, Alison T. Ung, Stephen G. Pyne
Faculty of Science - Papers (Archive)
Novel 2′-aminoalkyl-1-benzylisoquinoline compounds and medium size ring analogues have been prepared using reductive alkylation methods. Four of these analogues were tested for biological activity across 48 different CNS receptors and were showed to have binding activities at the mu opiod receptor.
The Acute Nociceptive Signals Induced By Bradykinin In Rat Sensory Neurons Are Mediated By Inhibition Of M-Type K+ Channels And Activation Of Ca2+-Activated Cl- Channels, Boyi Liu, John E. Linley, Xiaona Du, Xuan Zhang, Lezanne Ooi, Hailin Zhang, Nikita Gamper
The Acute Nociceptive Signals Induced By Bradykinin In Rat Sensory Neurons Are Mediated By Inhibition Of M-Type K+ Channels And Activation Of Ca2+-Activated Cl- Channels, Boyi Liu, John E. Linley, Xiaona Du, Xuan Zhang, Lezanne Ooi, Hailin Zhang, Nikita Gamper
Faculty of Science - Papers (Archive)
Bradykinin (BK) is an inflammatory mediator and one of the most potent endogenous pain-inducing substances. When released at sites of tissue damage or inflammation, or applied exogenously, BK produces acute spontaneous pain and causes hyperalgesia (increased sensitivity to potentially painful stimuli). The mechanisms underlying spontaneous pain induced by BK are poorly understood. Here we report that in small nociceptive neurons from rat dorsal root ganglia, BK, acting through its B2 receptors, PLC, and release of calcium from intracellular stores, robustly inhibits M-type K+ channels and opens Ca2+-activated Cl- channels (CaCCs) encoded by Tmem16a (also known as Ano1). Summation of these …
Transcriptional Control Of Kcnq Channel Genes And The Regulation Of Neuronal Excitability, Mariusz Mucha, Lezanne Ooi, John Linley, Pawel Mordaka, Carine Dalle, Brian Robertson, Nikita Gamper, Ian C. Wood
Transcriptional Control Of Kcnq Channel Genes And The Regulation Of Neuronal Excitability, Mariusz Mucha, Lezanne Ooi, John Linley, Pawel Mordaka, Carine Dalle, Brian Robertson, Nikita Gamper, Ian C. Wood
Faculty of Science - Papers (Archive)
Regulation of the resting membrane potential and the repolarization of neurons are important in regulating neuronal excitability. The potassium channel subunits Kv7.2 and Kv7.3 play a key role in stabilizing neuronal activity. Mutations in KCNQ2 and KCNQ3, the genes encoding Kv7.2 and Kv7.3, cause a neonatal form of epilepsy, and activators of these channels have been identified as novel antiepileptics and analgesics. Despite the observations that regulation of these subunits has profound effects on neuronal function, almost nothing is known about the mechanisms responsible for controlling appropriate expression levels. Here we identify two mechanisms responsible for regulating KCNQ2 and …
Essential Biological Processes Of An Emerging Pathogen: Dna Replication, Transcription, And Cell Division In Acinetobacter Spp., Andrew Robinson, Anthony J. Brzoska, Kylie M. Turner, Ryan Withers, Elizabeth J. Harry, Peter J. Lewis, Nicholas E. Dixon
Essential Biological Processes Of An Emerging Pathogen: Dna Replication, Transcription, And Cell Division In Acinetobacter Spp., Andrew Robinson, Anthony J. Brzoska, Kylie M. Turner, Ryan Withers, Elizabeth J. Harry, Peter J. Lewis, Nicholas E. Dixon
Faculty of Science - Papers (Archive)
Species of the bacterial genus Acinetobacter are becoming increasingly important as a source of hospital-acquired infections (31, 185, 204). Acinetobacter spp. are ubiquitous nonmotile gammaproteobacteria, typified by metabolic versatility and a capacity for natural transformation (172, 204). The species of most clinical relevance is A. baumannii; however, pathogenic strains of A. lwoffi and A. baylyi have also been described (38, 185, 215).
The Quaternary Organization And Dynamics Of The Molecular Chaperone Hsp26 Are Thermally Regulated, J. L. Benesch, Andrew Aquilina, Andrew Baldwin, Agata Rekas, Florian Stengel, Robyn Lindner, Eman Basha, G. Devlin, J. Horwitz, Elizabeth Vierling, John Carver, C. V. Robinson
The Quaternary Organization And Dynamics Of The Molecular Chaperone Hsp26 Are Thermally Regulated, J. L. Benesch, Andrew Aquilina, Andrew Baldwin, Agata Rekas, Florian Stengel, Robyn Lindner, Eman Basha, G. Devlin, J. Horwitz, Elizabeth Vierling, John Carver, C. V. Robinson
Faculty of Science - Papers (Archive)
The function of ScHSP26 is thermally controlled: the heat shock that causes the destabilization of target proteins leads to its activation as a molecular chaperone. We investigate the structural and dynamical properties of ScHSP26 oligomers through a combination of multiangle light scattering, fluorescence spectroscopy, NMR spectroscopy, and mass spectrometry. We show that ScHSP26 exists as a heterogeneous oligomeric ensemble at room temperature. At heat-shock temperatures, two shifts in equilibria are observed: toward dissociation and to larger oligomers. We examine the quaternary dynamics of these oligomers by investigating the rate of exchange of subunits between them and find that this not …
Preclinical Evaluation Of Novel All-In-One Formulations Of 5-Fluorouracil And Folinic Acid With Reduced Toxicity Profiles - Supplementary Data, Tamantha K. Stutchbury, Kara L. Vine, Julie M. Locke, Jeremy S. Chrisp, J. B. Bremner, P Clingan, Marie Ranson
Preclinical Evaluation Of Novel All-In-One Formulations Of 5-Fluorouracil And Folinic Acid With Reduced Toxicity Profiles - Supplementary Data, Tamantha K. Stutchbury, Kara L. Vine, Julie M. Locke, Jeremy S. Chrisp, J. B. Bremner, P Clingan, Marie Ranson
Faculty of Science - Papers (Archive)
Objectives: 5-Fluorouracil (5-FU) in combination with its synergistic biomodulator folinic acid maintains a pivotal position in cancer chemotherapy. However, clinical limitations persist with the administration of these drugs in combination including phlebitis and catheter blockages, which are associated with reduced efficacy and/or quality of life for patients. We have previously reported novel all-in-one, pH neutral, parenteral 5-FU and folinic acid formulations (termed Fluorodex) incorporating beta-cyclodextrins. Fluorodex maintains potency while overcoming the accepted incompatibility of 5-FU and folinic acid.
Methods: We performed toxicological, pharmacokinetic and biodistribution, and efficacy evaluations of Fluorodex compared to 5-FU:folinic acid using several administration routes …
Elastic Scattering Studies Of Aligned Dmpc Multilayers On Different Hydrations, Marcus Trapp, Fanni Juranyi, Moeava Tehei, Lambert Van Eijck, Bruno Demé, Thomas Gutberlet, Judith Peters
Elastic Scattering Studies Of Aligned Dmpc Multilayers On Different Hydrations, Marcus Trapp, Fanni Juranyi, Moeava Tehei, Lambert Van Eijck, Bruno Demé, Thomas Gutberlet, Judith Peters
Faculty of Science - Papers (Archive)
Biological membranes, consisting mainly of phospholipids and proteins, are organized in a bilayered structure which exhibits dynamical behaviour within time regimes ranging from 10-12 s with the motion of alkyl chain defects and 1 s corresponding to collective excitations of the bilayer [1]. With the prominent role hydration plays on the structural phase behaviour of phospholipids membranes, it is essential for a better description of membranes to understand also the influence of hydration on the dynamics of membrane systems. In the present study we have performed neutron scattering investigations on highly oriented DMPC-d54 multilayers at two different relative humidity (rh) …
Localization Of Low Molecular Weight Crystallin Peptides In The Aging Human Lens Using A Maldi Mass Spectrometry Imaging Approach, S. P. Su, Jason D. Mcarthur, J. A. Aquilina
Localization Of Low Molecular Weight Crystallin Peptides In The Aging Human Lens Using A Maldi Mass Spectrometry Imaging Approach, S. P. Su, Jason D. Mcarthur, J. A. Aquilina
Faculty of Science - Papers (Archive)
Low molecular weight (LMW) peptides, derived from the breakdown of the major eye lens proteins, the crystallins, accumulate in the human lens with age. These LMW peptides are associated with age-related lens opacity and cataract, with some shown to inhibit the chaperone activity of α-crystallin. However, the mechanism(s) giving rise to the production of these peptides, as well as their distribution within the lens, are not well understood. In this study, we have mapped the distribution of these crystallin-derived peptides present in human lenses of different ages using matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS). Our data showed that most of …
Synthesis Of Hyacinthacine B-3 And Purported Hyacinthacine B-7, Christopher W.G. Au, Robert J. Nash, Stephen G. Pyne
Synthesis Of Hyacinthacine B-3 And Purported Hyacinthacine B-7, Christopher W.G. Au, Robert J. Nash, Stephen G. Pyne
Faculty of Science - Papers (Archive)
The synthesis of hyacinthacines B-3 and B-7 has confirmed the structure of the former alkaloid and shown that the structure of the latter is incorrect.
A Mass Spectrometric Investigation Of Novel Quadruplex Dna-Selective Berberine Derivatives, Karina Gornall, Siritron Samosorn, Bongkot Tanwirat, Apichart Suksamrarn, John B. Bremner, Michael J. Kelso, Jennifer L. Beck
A Mass Spectrometric Investigation Of Novel Quadruplex Dna-Selective Berberine Derivatives, Karina Gornall, Siritron Samosorn, Bongkot Tanwirat, Apichart Suksamrarn, John B. Bremner, Michael J. Kelso, Jennifer L. Beck
Faculty of Science - Papers (Archive)
ESI mass spectrometry was used to assess the binding of 13-substituted, 5-nitro-2-phenylindolyl- and 2-naphthalenyl-based berberine derivatives to inter- and intramolecular G-quadruplex DNA molecules. In contrast with the parent berberine, the compounds showed selectivity for quadruplex over duplex DNA and stabilised the quadruplex structure. They represent a new class of quadruplex DNA-selective ligands. © 2010 The Royal Society of Chemistry.
Copper-Mediated Cyclization-Halogenation And Cyclization-Cyanation Reactions Of Β-Hydroxyalkynes And O-Alkynylphenols And Anilines, Nalivela K. Swamy, Arife Yazici, Stephen G. Pyne
Copper-Mediated Cyclization-Halogenation And Cyclization-Cyanation Reactions Of Β-Hydroxyalkynes And O-Alkynylphenols And Anilines, Nalivela K. Swamy, Arife Yazici, Stephen G. Pyne
Faculty of Science - Papers (Archive)
The CuX (X = I, Br, Cl, CN)-mediated cyclization-halogenation and cyclization-cyanation reactions of β-hydroxyalkynes and o-alkynylphenol and -aniline derivatives give rise to 3-halo- and 3-cyanofuro[3,2-b]pyrroles, 3-iodo-, 3-bromo-, and 3-cyanobenzofurans, and 3-cyanoindoles, respectively. © 2010 American Chemical Society.
Dipyridyl Beta-Diketonate Complexes: Versatile Polydentate Metalloligands For Metal-Organic Frameworks And Hydrogen-Bonded Networks, Andrew D. Burrows, Christopher G. Frost, Mary F. Mahon, Paul R. Raithby, Catherine L. Renouf, Christopher Richardson, Anna J. Stevenson
Dipyridyl Beta-Diketonate Complexes: Versatile Polydentate Metalloligands For Metal-Organic Frameworks And Hydrogen-Bonded Networks, Andrew D. Burrows, Christopher G. Frost, Mary F. Mahon, Paul R. Raithby, Catherine L. Renouf, Christopher Richardson, Anna J. Stevenson
Faculty of Science - Papers (Archive)
The Group 13 metal complexes [M(L-2)(3)], where M is Al or Ga and L-2 is 1,3-di(4-pyridyl)-1,3-propanedionato, are hexatopic metalloligands that have been used to prepare mixed-metalorganic frameworks containing interpenetrated primitive cubic networks. In contrast, the europium complex [Eu(HL2)(3)-(H2L2)]Cl-4 center dot EtOH forms a hydrogen-bonded network following partial protonation of the pyridyl groups.
Equilibrium And Kinetic Modeling Of The Adsorption Of Indigo Carmine Onto Silk, Naparat Jiwalak, Saowanee Rattanaphani, John B. Bremner, Vichitr Rattanaphani
Equilibrium And Kinetic Modeling Of The Adsorption Of Indigo Carmine Onto Silk, Naparat Jiwalak, Saowanee Rattanaphani, John B. Bremner, Vichitr Rattanaphani
Faculty of Science - Papers (Archive)
Quantitative adsorption kinetic and equilibrium parameters for indigo carmine dyeing of silk were studied using UV-visible absorption spectroscopy. The effect of initial dye concentration, contact time, pH, material to liquor ratio (MLR), and temperature were determined to find the optimal conditions for adsorption. The mechanism of adsorption of indigo carmine dyeing onto silk was investigated using the pseudo first-order and pseudo second-order kinetic models. The adsorption kinetics was found to follow a pseudo-second-order kinetic model with an activation energy (E-a) of 51.06 kJ/mol. The equilibrium adsorption data of indigo carmine dye on silk were analyzed by the Langmuir and Freundlich …
Esi-Ms And Thermal Melting Studies Of Nanoscale Platinum(Ii) Metallomacrocycles With Dna, Margaret M. Harding, Louis M. Rendina, David Schilter, Thitima Urathamakul, Jennifer L. Beck
Esi-Ms And Thermal Melting Studies Of Nanoscale Platinum(Ii) Metallomacrocycles With Dna, Margaret M. Harding, Louis M. Rendina, David Schilter, Thitima Urathamakul, Jennifer L. Beck
Faculty of Science - Papers (Archive)
The hydrophilic, long-chain diamine PEGda (O,O′-bis(2-aminoethyl)octadeca(ethylene glycol)), when complexed with cis-protected Pt(II) ions afforded water-soluble complexes of the type [Pt(N,N)(PEGda)](NO3)2 (N,N = N,N,N′,N′-tetramethyl-1,2-diaminoethane (tmeda), 1,2-diaminoethane (en), and 2,2′-bipyridine (2,2′-bipy)) featuring unusual 62-membered chelate rings. Equimolar mixtures containing either the 16-mer duplex DNA D2 or the single-stranded D2a and [Pt(N,N)(PEGda)]2+ were analyzed by negative-ion ESI-MS. Analysis of D2-Pt(II) mixtures showed the formation of 1:1 adducts of [Pt(en)(PEGda)]2+, [Pt(tmeda)(PEGda)]2+ and the previously-described metallomacrocycle [Pt2(2,2′-bipy)2{4,4′-bipy(CH2)44,4′-bipy}2]8+ with D2; the dinuclear species bound to D2 most strongly, consistent with its greater charge and aromatic surface area. D2 formed 1:2 complexes with the acyclic species [Pt(2,2′-bipy)(Mebipy)2]4+ …
Misidentification Of Tansy, Tanacetum Macrophyllum, As Yarrow, Achillea Grandifolia: A Health Risk Or Benefit?, Niko Radulovic, Polina Blagojevic, Danielle Skropeta, Aleksandra Zarubica, Bojan Zlatkovic, Radosav Palic´
Misidentification Of Tansy, Tanacetum Macrophyllum, As Yarrow, Achillea Grandifolia: A Health Risk Or Benefit?, Niko Radulovic, Polina Blagojevic, Danielle Skropeta, Aleksandra Zarubica, Bojan Zlatkovic, Radosav Palic´
Faculty of Science - Papers (Archive)
Tansy, Tanacetum macrophyllum (Waldst. & Kit.) Sch. Bip., is often misidentified by herb collectors as yarrow, Achillea grand folia Friv. With the former, cases of poisoning induced by its ingestion are well documented, but the latter is widely used for ethnopharmacological purposes. The aim of this study was to estimate, based on the volatile metabolite profiles of the two species, the potential health risk connected with their misidentification. GC and GC-MS analysis of the essential oils hydrodistilled using a Clevenger-type apparatus from A. grandifolia, T macrophyllum, and two plant samples (reputedly of A. grandifolia, but in fact mixtures of A. …
Spatial Variability Of Cadmium, Copper, Manganese, Nickel And Zinc In The Port Curtis Estuary, Queensland, Australia, Brad M. Angel, Leigh T. Hales, Stuart L. Simpson, Simon C. Apte, Anthony A. Chariton, Damon A. Shearer, Dianne F. Jolley
Spatial Variability Of Cadmium, Copper, Manganese, Nickel And Zinc In The Port Curtis Estuary, Queensland, Australia, Brad M. Angel, Leigh T. Hales, Stuart L. Simpson, Simon C. Apte, Anthony A. Chariton, Damon A. Shearer, Dianne F. Jolley
Faculty of Science - Papers (Archive)
Port Curtis is a rapidly growing industrialised and urbanised harbour in Central Queensland, Australia. Water sampling surveys were undertaken in late 2003 and 2004, accompanied by additional sediment sampling to investigate the sources and behaviour of trace metals, and the effects of pH on metal partitioning between dissolved and particulate forms. Sampling and analyses of trace metals in waters and suspended particulates were undertaken along axial transects extending away from possible point-sources within the harbour. Additional sampling was undertaken in selected inlets and major freshwater sources to Port Curtis, including the Fitzroy River. Most dissolved metal concentrations were significantly elevated …
Novel Technique Shows Different Hydrophobic Chemical Signatures Of Exotic And Indigenous Plant Soils With Similar Effects Of Extracts On Indigenous Species Seedling Growth, Emilie-Jane Ens, Kristine O. French, John B. Bremner, Jurgen Korth
Novel Technique Shows Different Hydrophobic Chemical Signatures Of Exotic And Indigenous Plant Soils With Similar Effects Of Extracts On Indigenous Species Seedling Growth, Emilie-Jane Ens, Kristine O. French, John B. Bremner, Jurgen Korth
Faculty of Science - Papers (Archive)
Changes to ecosystem abiotic parameters are regarded as possible mechanisms facilitating plant invasion and community composition shifts. This study compared the hydrophobic chemical signatures of soil from exotic bitou bush (Chrysanthemoides monilifera spp. rotundata) invaded, indigenous acacia (Acacia longifolia var. sophorae) dominated and bare sand (unvegetated) habitats using a novel, rapid, capturing technique which utilised AmberliteA (R) XAD4 resin filled bags that were placed in situ. The hydrophobic chemical signature of the bitou bush soil extract was significantly different to the acacia soil and bare sand extracts. High concentrations of 18 sesquiterpenes dominated the hydrophobic signature of the bitou bush …
Specific Cellular Water Dynamics Observed In Vivo By Neutron Scattering And Nmr., Moeava Tehei, Marion Jasnin, Andreas Stadler, Giuseppe Zaccai
Specific Cellular Water Dynamics Observed In Vivo By Neutron Scattering And Nmr., Moeava Tehei, Marion Jasnin, Andreas Stadler, Giuseppe Zaccai
Faculty of Science - Papers (Archive)
Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (Ba˚ ngstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not ‘‘tamed’’ by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration …
Hydration Dependent Studies Of Highly Aligned Multilayer Lipid Membranes By Neutron Scattering , Marcus Trapp, Thomas Gutberlet, Fanni Juranyi, T Unruh, Bruno Demé, Moeava Tehei, Judith Peters
Hydration Dependent Studies Of Highly Aligned Multilayer Lipid Membranes By Neutron Scattering , Marcus Trapp, Thomas Gutberlet, Fanni Juranyi, T Unruh, Bruno Demé, Moeava Tehei, Judith Peters
Faculty of Science - Papers (Archive)
We investigated molecular motions on a picoseconds timescale of 1,2-Dimyristoyl-sn- Glycero-3-Phosphocholine (DMPC) model membranes as a function of hydration by using elastic and quasielastic neutron scattering. Two different hydrations corresponding to approximately nine and twelve water molecules per lipid were studied, the latter being the fully hydrated state. In our study we focused on head group motions by using chain deuterated lipids. Information on in-plane and out-of-plane motions could be extracted by using solid supported DMPC multilayers. Our studies confirm and complete former investigations by K¨onig et al. and Rheinst¨adter et al. who describe the dynamics of lipidmembranes, but did …
Average Mass Scan Of The Total Ion Chromatogram Versus Percentage Chemical Composition In Multivariate Statistical Comparison Of Complex Volatile Mixtures, Niko Radulovic, Polina Blagojevic, Danielle Skropeta
Average Mass Scan Of The Total Ion Chromatogram Versus Percentage Chemical Composition In Multivariate Statistical Comparison Of Complex Volatile Mixtures, Niko Radulovic, Polina Blagojevic, Danielle Skropeta
Faculty of Science - Papers (Archive)
The analysis of complex volatile mixtures by gas chromatography-mass spectrometry (GC-MS) is a time-consuming process. It involves separation and identification of the components based on their retention times and fragmentation patterns, followed by determination of their relative percentages from integration of their peak areas. Herein we show that multivariate statistical analysis of the relative abundances of the m/z values obtained from the average mass scans (AMS) of the complex mixture is a faster and potentially more reliable method of assessing these mixtures. To achieve this, 15 model complex mixtures, were prepared comprising varying amounts of 10 different constituents. The AMS …
Learning How To Be An Academic - The Story Of A New Academic In The Sciences, Danielle Skropeta
Learning How To Be An Academic - The Story Of A New Academic In The Sciences, Danielle Skropeta
Faculty of Science - Papers (Archive)
No abstract provided.
Extracellular Chaperones Modulate The Effects Of Alzheimer's Patient Cerebrospinal Fluid On A Beta(1-42) Toxicity And Uptake , Justin J. Yerbury, Mark R. Wilson
Extracellular Chaperones Modulate The Effects Of Alzheimer's Patient Cerebrospinal Fluid On A Beta(1-42) Toxicity And Uptake , Justin J. Yerbury, Mark R. Wilson
Faculty of Science - Papers (Archive)
Alzheimer's disease is characterised by the inappropriate death of brain cells and accumulation of the A beta peptide in the brain. Thus, it is possible that there are fundamental differences between Alzheimer's disease patients and healthy individuals in their abilities to clear A beta from brain fluid and to protect neurons from A beta toxicity. In the present study, we examined (1) the cytotoxicity of Alzheimer's disease cerebrospinal fluid (CSF) compared to control CSF, (2) the ability of Alzheimer's disease and control CSF to protect cells from A beta toxicity and to promote cell-mediated clearance of A beta and lastly …
Total Synthesis Of Uniflorine A, Casuarine, Australine, 3-Epi-Australine, And 3,7-Di-Epi-Australine From A Common Precursor, Thunwadee Ritthiwigrom, Anthony C. Willis, Stephen G. Pyne
Total Synthesis Of Uniflorine A, Casuarine, Australine, 3-Epi-Australine, And 3,7-Di-Epi-Australine From A Common Precursor, Thunwadee Ritthiwigrom, Anthony C. Willis, Stephen G. Pyne
Faculty of Science - Papers (Archive)
A flexible method for the diastereoselective total synthesis of the pyrrolizidine alkaloids Uniflorine A, casuarine, australine, and 3-epi-australine and the unnatural epimer 3,7-di-epi-australine from a common chiral 2,5-dihydropyrrole precursor is described.
Parameters Governing Invasive Disease Propensity Of Non-M1 Serotype Group A Streptococci, Peter G. Maamary, Martina L. Sanderson-Smith, Ramy K. Aziz, Andrew Hollands, Jason N. Cole, Fiona C. Mckay, Jason D. Mcarthur, Joshua K. Kirk, Amanda J. Cork, Rachael J. Keefe, Rita G. Kansal, Hongmin Sun, William L. Taylor, Gursharan S. Chhatwal, David Ginsburg, Victor Nizet, Malak Kotb, Mark J. Walker
Parameters Governing Invasive Disease Propensity Of Non-M1 Serotype Group A Streptococci, Peter G. Maamary, Martina L. Sanderson-Smith, Ramy K. Aziz, Andrew Hollands, Jason N. Cole, Fiona C. Mckay, Jason D. Mcarthur, Joshua K. Kirk, Amanda J. Cork, Rachael J. Keefe, Rita G. Kansal, Hongmin Sun, William L. Taylor, Gursharan S. Chhatwal, David Ginsburg, Victor Nizet, Malak Kotb, Mark J. Walker
Faculty of Science - Papers (Archive)
Group A Streptococcus (GAS) causes rare but life-threatening syndromes of necrotizing fasciitis and toxic shock-like syndrome in humans. The GAS serotype M1T1 clone has globally disseminated, and mutations in the control of virulence regulatory sensor kinase (covRS) operon correlate with severe invasive disease. Here, a cohort of non-M1 GAS was screened to determine whether mutation in covRS triggers systemic dissemination in divergent M serotypes. A GAS disease model defining parameters governing invasive propensity of differing M types is proposed. The vast majority of GAS infection is benign. Nonetheless, many divergent M types possess limited capacity to cause invasive infection. Mill …
Phytochemical Investigations Of Stemona Curtisii And Synthetic Studies On Stemocurtisine Alkaloids, Sukanda Chaiyong, Araya Jatisatienr, Pitchaya Mungkornasawakul, Thanapat Sastraruji, Stephen G. Pyne, Alison T. Ung, Thitima Urathamakul, Wilford Lie
Phytochemical Investigations Of Stemona Curtisii And Synthetic Studies On Stemocurtisine Alkaloids, Sukanda Chaiyong, Araya Jatisatienr, Pitchaya Mungkornasawakul, Thanapat Sastraruji, Stephen G. Pyne, Alison T. Ung, Thitima Urathamakul, Wilford Lie
Faculty of Science - Papers (Archive)
The isolation of two new Stemona alkaloids, 1-hydroxyprotostemonine and stemocurtisine N-oxide, and a new benzofuran, stemofuran L, from the root extracts of Stemona curtisii is reported. The major known alkaloids from this plant extract, stemocurtisine, stemocurtisinol, and oxyprotostemonine, were also isolated along with oxystemokerrine N-oxide. The nonalkaloid components of this extract included a new benzofuran derivative, stemofuran L, the known stemofurans F, J, and K, dihydro-γ-tocopherol, and stigmasterol. Stemocurtisine and stemocurtisinol were converted to their respective N-oxides by oxidation. Stemocurtisine was converted to a tetracyclic derivative by oxidative cleavage of the γ-butyrolactone ring, while stemocurtisinol gave a novel lactam derivative …