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Full-Text Articles in Social and Behavioral Sciences
Specific Cellular Water Dynamics Observed In Vivo By Neutron Scattering And Nmr., Moeava Tehei, Marion Jasnin, Andreas Stadler, Giuseppe Zaccai
Specific Cellular Water Dynamics Observed In Vivo By Neutron Scattering And Nmr., Moeava Tehei, Marion Jasnin, Andreas Stadler, Giuseppe Zaccai
Faculty of Science - Papers (Archive)
Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (Ba˚ ngstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not ‘‘tamed’’ by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration …
Hydration Dependent Studies Of Highly Aligned Multilayer Lipid Membranes By Neutron Scattering , Marcus Trapp, Thomas Gutberlet, Fanni Juranyi, T Unruh, Bruno Demé, Moeava Tehei, Judith Peters
Hydration Dependent Studies Of Highly Aligned Multilayer Lipid Membranes By Neutron Scattering , Marcus Trapp, Thomas Gutberlet, Fanni Juranyi, T Unruh, Bruno Demé, Moeava Tehei, Judith Peters
Faculty of Science - Papers (Archive)
We investigated molecular motions on a picoseconds timescale of 1,2-Dimyristoyl-sn- Glycero-3-Phosphocholine (DMPC) model membranes as a function of hydration by using elastic and quasielastic neutron scattering. Two different hydrations corresponding to approximately nine and twelve water molecules per lipid were studied, the latter being the fully hydrated state. In our study we focused on head group motions by using chain deuterated lipids. Information on in-plane and out-of-plane motions could be extracted by using solid supported DMPC multilayers. Our studies confirm and complete former investigations by K¨onig et al. and Rheinst¨adter et al. who describe the dynamics of lipidmembranes, but did …
Dynamics Of Apomyoglobin In The Α-To-Β Transition And Of Partially Unfolded Aggregated Protein, E. Fabiani, A. M. Stadler, D. Madern, M. M. Koza, M. Tehei, M. Hirai, G. Zaccai
Dynamics Of Apomyoglobin In The Α-To-Β Transition And Of Partially Unfolded Aggregated Protein, E. Fabiani, A. M. Stadler, D. Madern, M. M. Koza, M. Tehei, M. Hirai, G. Zaccai
Faculty of Science - Papers (Archive)
Changes of molecular dynamics in the α-to-β transition associated with amyloid fibril formation were explored on apo-myoglobin (ApoMb) as a model system. Circular dichroism, neutron and X-ray scattering experiments were performed as a function of temperature on the protein, at different solvent conditions. A significant change in molecular dynamics was observed at the α-to-β transition at about 55 ˚C, indicating a more resilient high temperature β structure phase. A similar effect at approximately the same temperature was observed in holo-myoglobin, associated with partial unfolding and protein aggregation. A study in a wide temperature range between 20 K and 360 K …
Dynamics Of Immobilized And Native Escherichia Coli Dihydrofolate Reductase By Quasielastic Neutron Scattering, M. Tehei, J. Smith, C. Monk, J. Ollivier, M. Oettl, V. Kurkal, J. L. Finney, R. M. Daniel
Dynamics Of Immobilized And Native Escherichia Coli Dihydrofolate Reductase By Quasielastic Neutron Scattering, M. Tehei, J. Smith, C. Monk, J. Ollivier, M. Oettl, V. Kurkal, J. L. Finney, R. M. Daniel
Faculty of Science - Papers (Archive)
The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering. These results reveal no difference between the high frequency vibration mean-square displacement of the native and the immobilized E. coli DHFR. However, length-scale-dependent, picosecond dynamical changes are found. On longer length scales, the dynamics are comparable for both DHFR samples. On shorter length scales, the dynamics is dominated by local jump motions over potential barriers. The residence time for the protons to stay in a potential well is t=7.95 ps for the native DHFR and t=20.36 ps for the immobilized …