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Full-Text Articles in Social and Behavioral Sciences
Discovery Of A Functional Ecdysone Response Element In Brugia Malayi, Tracy Enright
Discovery Of A Functional Ecdysone Response Element In Brugia Malayi, Tracy Enright
USF Tampa Graduate Theses and Dissertations
The aim of this study was to determine whether functional ecdysone response elements (EcREs) exist within the genome of Brugia malayi, a parasitic nematode that causes lymphatic filariasis. The hypothesis that EcREs exist in B. malayi stemmed from previous demonstration of a functional ecdysone response system in B. malayi (Tzertzinis et al., 2010). Real-time PCR (qPCR) experiments were conducted to measure gene expression levels for twelve genes proximal to five putative EcREs in 20-OH ecdysone treated and untreated B. malayi embryos. Seven genes showed consistent upregulation with 20-OH ecdysone treatment. Each of the five putative EcREs had at least one …
Core Promoter Function In Brugia Malayi, Michelle Bailey
Core Promoter Function In Brugia Malayi, Michelle Bailey
USF Tampa Graduate Theses and Dissertations
Previous studies have indicated that the promoters of the human filarial parasite Brugia malayi are unusual in that they do not exhibit the CAAT or TATAA sequences usually found in the core domains of promoters of most eukaryotic organisms. Analysis of the promoters of the ribosomal proteins showed that the region flanking the splice leader (SL) addition site plays an important role in transcription and may function as the core promoter domain in B. malayi. To test the hypothesis that the SL addition domain is the most important essential region of the ribosomal protein promoters, the SL addition site of …