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Social and Behavioral Sciences Commons

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Physical Sciences and Mathematics

University of Nebraska - Lincoln

School of Natural Resources: Faculty Publications

2003

Articles 1 - 2 of 2

Full-Text Articles in Social and Behavioral Sciences

Lewis And Clark And The Geology Of The Great Plains, Robert F. Diffendal Jr., Anne P. Diffendal Dec 2003

Lewis And Clark And The Geology Of The Great Plains, Robert F. Diffendal Jr., Anne P. Diffendal

School of Natural Resources: Faculty Publications

Meriwether Lewis and William Clark undertook their journey with the Corps of Discovery in 1804-1806 in order to explore the area that the United States had purchased from France in 1803. Then known as Louisiana, this region included almost everything west of the Mississippi to the continental divide. In order to find the best route across the continent, President Thomas Jefferson charged Lewis with following the Missouri River to its headwaters and then locating rivers flowing down the west side of the Rocky Mountains to the Columbia River and into the Pacific Ocean. Jefferson's written instructions further specified that the …


Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente Feb 2003

Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente

School of Natural Resources: Faculty Publications

A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.