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Full-Text Articles in Social and Behavioral Sciences
Assessing The Archaeoparasitological Potential Of Quids As A Source Material For Immunodiagnostic Analyses, Johnica J. Morrow, Karl Reinhard
Assessing The Archaeoparasitological Potential Of Quids As A Source Material For Immunodiagnostic Analyses, Johnica J. Morrow, Karl Reinhard
Karl Reinhard Publications
In the present study, quids from La Cueva de los Muertos Chiquitos (CMC) were subjected to ELISA tests for 2 protozoan parasites, Toxoplasma gondii (n=45) and Trypanosoma cruzi (n=43). The people who occupied CMC, the Loma San Gabriel, lived throughout much of present-day Durango and Zacatecas in Mexico. The known pathoecology of these people puts them into at-risk categories for the transmission of T. gondii and T. cruzi. Human antibodies created in response to these 2 parasites can be detected in modern saliva using ELISA kits intended for use with human serum. For these reasons, quids were reconstituted and …
Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira
Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira
Karl Reinhard Publications
The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human feces. We utilized the assay in ancient fecal material. The material included desiccated feces found in mummies or in archaeological sites, and sediments from latrines. A total of 83 specimens, previously examined microscopically for parasites, were examined. The ELISA detected 3 positive samples, dated to about 1200 AD, 1600 AD, and 1700 AD. …