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Articles 1 - 5 of 5
Full-Text Articles in Social and Behavioral Sciences
Truncation, Cross-Linking And Interaction Of Crystallins And Intermediate Filament Proteins In The Aging Human Lens, Roger Truscott, Jason Mcarthur, Andrew Aquilina, Shi-Ping (Jim) Su
Truncation, Cross-Linking And Interaction Of Crystallins And Intermediate Filament Proteins In The Aging Human Lens, Roger Truscott, Jason Mcarthur, Andrew Aquilina, Shi-Ping (Jim) Su
J. A. Aquilina
The optical properties of the lens are dependent upon the integrity of proteins within the fiber cells. During aging, crystallins, the major intra-cellular structural proteins of the lens, aggregate and become water-insoluble. Modifications to crystallins and the lens intermediate filaments have been implicated in this phenomenon. In this study, we examined changes to, and interactions between, human lens crystallins and intermediate filament proteins in lenses from a variety of age groups (0-86 years). Among the lens-specific intermediate filament proteins, filensin was extensively cleaved in all postnatal lenses, with truncated products of various sizes being found in both the lens cortical …
Chemical Cross-Linking Of The Chloroplast Localized Small Heat-Shock Protein, Hsp21, And The Model Substrate Citrate Synthase, Emma Ahrman, W Lambert, Andrew Aquilina, C V Robinson, Cs Emanuelsson
Chemical Cross-Linking Of The Chloroplast Localized Small Heat-Shock Protein, Hsp21, And The Model Substrate Citrate Synthase, Emma Ahrman, W Lambert, Andrew Aquilina, C V Robinson, Cs Emanuelsson
J. A. Aquilina
The molecular mechanism whereby the small heat-shock protein (sHsp) chaperones interact with and prevent aggregation of other proteins is not fully understood. We have characterized the sHsp-substrate protein interaction at normal and increased temperatures utilizing a model substrate protein, citrate synthase (CS), widely used in chaperone assays, and a dodecameric plant sHsp, Hsp21, by chemical cross-linking with 3,3'-Dithiobis[sulfosuccinimidylpropionate] (DTSSP) and mass spectrometric peptide mapping. In the absence of CS, the cross-linker captured Hsp21 in dodecameric form, even at increased temperature (47 degrees C). In the presence of equimolar amounts of CS, no Hsp21 dodecamer was captured, indicating a substrate-induced Hsp21 …
The Effect Of Detraining On Muscle Strength And Cross-Sectional Area Following Unilateral Resistance Training, Herbert Groeller, John Sampson
The Effect Of Detraining On Muscle Strength And Cross-Sectional Area Following Unilateral Resistance Training, Herbert Groeller, John Sampson
John Sampson
Muscle strength appears well preserved following short periods of detraining, despite a decline in muscle cross-sectional area and muscle activation. PURPOSE: This study determined the effect a 12-week unilateral elbow flexor resistance training regimen followed by 8 weeks of detraining on muscle cross-sectional area, muscle activation, and dynamic and static strength in trained and non-exercising contralateral limbs. METHODS: Ten males volunteered for the investigation and completed a 4-week pre-treatment training period (50-80%1RM) prior to commencing the experimental resistance training regimen. Subjects attended 3 sessions per week over 12 weeks of experimental training wherein they exercised at 85%1RM. Each subjects trained …
Conservation Planning In A Cross-Cultural Context: The Wunambal Gaambera Healthy Country Project In The Kimberley, Western Australia, Heather Moorcroft, Emma Ignjic, Stuart Cowell, John Goonack, Sylvester Mangolomara, Janet Oobagooma, Regina Karadada, Dianna Williams, Neil Waina
Conservation Planning In A Cross-Cultural Context: The Wunambal Gaambera Healthy Country Project In The Kimberley, Western Australia, Heather Moorcroft, Emma Ignjic, Stuart Cowell, John Goonack, Sylvester Mangolomara, Janet Oobagooma, Regina Karadada, Dianna Williams, Neil Waina
Faculty of Science - Papers (Archive)
This article illustrates how a conservation planning approach combined Indigenous knowledge and Western science to support Indigenous Traditional Owners to make decisions about managing their ancestral lands and seas, and communicate more strategically with external stakeholders.
Gelapin, A Degradable Genipin Cross-Linked Gelatin Hydrogel, Damian Kirchmajer, C Watson, Marie Ranson, Marc In Het Panhuis
Gelapin, A Degradable Genipin Cross-Linked Gelatin Hydrogel, Damian Kirchmajer, C Watson, Marie Ranson, Marc In Het Panhuis
Faculty of Science - Papers (Archive)
The synthesis of genipin cross-linked gelatin (Gelapin) hydrogel materials is presented. Gelapin hydrogels were comprehensively characterised through chemical, mechanical and physical analysis techniques. It was found that the hydrogels could be cross-linked to up to 90% using a genipin concentration of 24.4% (w/ w). The hydrogels reach a stable swollen state and cease leaching of residual starting materials after 72 h in phosphate buffered saline solution at 37 uC. The proteolytic degradation of Gelapin by collagenase is tuneable through manipulation of the material composition with the rate of degradation ranging from 60 mg per day up to 500 mg per …