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Predicting Off-Target Potential Of Crispr-Cas9 Single Guide Rna, Ishita Mathur
Predicting Off-Target Potential Of Crispr-Cas9 Single Guide Rna, Ishita Mathur
Master's Projects
With advancements in the field of genome engineering, researchers have come up with potential ways for site-specific gene editing. One of the methods uses the Clustered Regularly Interspaced Short Palindromic Repeats - CRISPR-Cas technology. It consists of a Cas9 nuclease and a single guide RNA (sgRNA) that cleaves the DNA at the intended target site. However, the target genome could contain multiple potential off-target sites and cleaving an off-target site can have deleterious effects in case of gene editing in humans.
Lab based assays have been developed to test the off-target effects of guide RNAs. However, it is not feasible …