Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Physical Sciences and Mathematics
Investigation In To The Stabilizing Effects Of The Modified Base Archaeosine In Trna And The Identification Of The Fluorescent Product Of Base Treatment Of Nad(P)+ Cofactors, Ben Turner
Dissertations and Theses
This dissertation covers two projects linked by their involvement in the modification of tRNA bases.
The first project focused on an investigation of a role for the modified base Archaeosine, the ubiquitous modification in tRNA in the archaeal domain. Initial work was performed on a set of in vitro prepared tRNA modified to feature either the canonical guanine base at position 15, preQ0 (TGT product) or Archaeosine (ArcS product). There was very little difference in the thermal stability of tRNAs containing these modifications in the halophilic H. volcanii tRNASer or E. coli tRNAGln. In tRNAGln …