Physical Sciences and Mathematics Commons^™

Stability Of Spore-Based Sensing Systems, Abhishek Sangal

University of Kentucky Master's Theses

The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long term-preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature.

In the present research, we …

Tectonic Controls On Lower Devonian Sandstone Distribution, Alabama, Michael P. Solis

University of Kentucky Master's Theses

The Devonian Frog Mountain Formation thickens abruptly eastward across the Eastern Coosa thrust fault from <12 m on the west to>70 m on the east. The thin Frog Mountain on the west unconformably overlies the Cambrian-Ordovician Knox Group. The thin Frog Mountain (mostly shale) is overlain by the Mississippian Maury Shale (~1 m thick) and Fort Payne Chert (~50 m thick). The thick Frog Mountain on the east rests on the Middle Ordovician Athens Shale, a black shale >150 m thick. The Athens overlies the Knox Group. The thick Frog Mountain is nearly all sandstone and is overlain by Fort Payne Chert which is only …

Functionalization Of Multi-Walled Carbon Nanotubes And The Interactions Of Multi-Walled Carbon Nanotubes In Epoxy Composites, Christopher Fitzwater

University of Kentucky Master's Theses

Multi-walled carbon nanotubes (MWNTs) are a relatively new allotrope of carbon that have potentially useful properties that may improve polymer composites. The work of this thesis explores the interactions between MWNTs and functionalized MWNTs within epoxy matrix and the properties of the MWNT/epoxy composite. These interactions were characterized with an emphasis on finding how well the MWNT/epoxy composite flows and how conductive it is after curing.

Origin Of Blue Ridge Basement Rocks, Dellwood Quad, Western Nc: New Evidence From U-Pb Zircon Geochronology And Whole Rock Geochemistry, Donald Franklin Loughry Jr.

University of Kentucky Master's Theses

Terrane discrimination in polycyclic continental basement rocks is challenging due to high-grade metamorphism and intense deformation. Based on early USGS mapping the Blue Ridge basement in the Dellwood quadrangle of the eastern Great Smoky Mountains was proposed to consist of augen orthogneisses of Laurentian (Grenvillian) affinity interfolded with migmatitic hornblende and biotite paragneisses (“Carolina Gneiss”) and amphibolites of uncertain affinity. However, detailed study reveals that the hornblende gneiss of Hadley and Goldsmith (1963) is a heterogeneous map unit consisting of (1)metaplutonic rocks; (2) variably foliated and folded felsic orthogneisses; (3) strongly migmatitic, folded Hbl+Bt-bearing gneisses; (4) foliated and lineated garnet …

Synthesis, Characterization And Development Of Catalysts For Co2 Capture, Anitha Suhas Wishrojwar

University of Kentucky Master's Theses

Fossil fuel and advanced industrialization techniques contribute to global warming through emissions of greenhouse gases such as CO₂. In order to mitigate climate change, there is a desperate need to reduce CO₂ emissions from different sources. CO₂ capture and sequestration (CCS) play an important role in these reductions.

Naturally occurring enzymes, e.g., carbonic anhydrase (CA), can catalyze these reactions in living systems. Much effort has been focused on complexes of zinc with ligands such as teta, cyclen and tripodal ligands including BIMA and Trispyrazolylborates. These complexes have many interesting CO₂ capture properties, but maintain toxic …

Effects Of Livestock Antibiotics On Nitrification, Denitrification, And Microbial Community Compositon In Soils Along A Topographic Gradient, Sagarika Banerjee

University of Kentucky Master's Theses

Several types of antibiotics (roxarsone, virginiamycin, and bacitracin) are widely included in poultry feed to improve animal growth yields. Most of the antibiotics are excreted in manure which is subsequently applied to soils. One concern with this practice is that antibiotics may affect several microbially-mediated nutrient cycling reactions in soils that influence crop productivity and water quality. The main objectives of this study were to determine the effects of livestock antibiotics on nitrification, denitrification, and microbial community composition in soils along a topographic gradient. These objectives were addressed in a series of lab experiments by monitoring changes in inorganic N …

Feasibility Of Using 15N-Enriched Escherichia Coli As A Bacterial Tracer In The Cane Run/Royal Spring Basin, Kentucky, John G. Warden

University of Kentucky Master's Theses

A novel tracer method has used ¹⁵N to label Escherichia coli and track the transport of bacteria, a common contaminant, through karst aquifers. Use of this method could provide valuable insight into the movement of bacteria in aquifers, which would help improve remediation methods and strategies. A wild strain of E. coli was isolated from the Cane Run/Royal Spring basin in the Inner Bluegrass region of Kentucky. The strain was serotyped O^-:H^- and virulence testing showed the strain did not have virulence factors of E. coli commonly pathogenic to humans. Five karst microcosms were filled with …

Chromium, Copper, And Arsenic Concentration And Speciation In Soil Adjacent To Chromated Copper Arsenate (Cca) Treated Lumber Along A Topohydrosequence, Donald Roy Schwer Iii

University of Kentucky Master's Theses

Arsenic (As), Chromium (Cr), and Copper (Cu) are ubiquitous in soils as a result of anthropogenic and geogenic processes. The fate of As, Cr, and Cu in the environment is largely governed by their speciation, which is influenced by soil physiochemical properties. This study investigated the influence of soil physiochemical properties and landscape position on As, Cr, and Cu concentration and speciation in soils adjacent to Chromated Copper Arsenate (CCA) treated lumber fence posts. Concentration gradients showed elevated total As and Cu adjacent to the three fence posts, which decreased with increasing distance from the posts. In addition, As and …

A Trusted Storage System For The Cloud, Sushama Karumanchi

University of Kentucky Master's Theses

Data stored in third party storage systems like the cloud might not be secure since confidentiality and integrity of data are not guaranteed. Though cloud computing provides cost-effective storage services, it is a third party service and so, a client cannot trust the cloud service provider to store its data securely within the cloud. Hence, many organizations and users may not be willing to use the cloud services to store their data in the cloud until certain security guarantees are made. In this thesis, a solution to the problem of securely storing the client’s data by maintaining the confidentiality and …

The No. 5 Block In Eastern Kentucky: A Critical Re-Examination Of The Petrology With Special Attention To The Origin Of Inertinite Macerals In The Splint Lithotypes, Allison Ranae Richardson

University of Kentucky Master's Theses

Microbes, including fungi and bacteria, and insects are responsible for the consumption and subsequent degradation of plant materials into humus. These microbes directly and indirectly affect the physical and chemical characteristics of coal macerals. Efforts to understand and determine the origins of inertinite macerals are largely misrepresented in the literature, conforming to a single origin of fire. This study focuses on the variability of physical and inferred chemical differences observed petrographically between the different inertinite macerals and discusses the multiple pathways plant material may take to form and or degrade these macerals.

Petrographic results show that fungal activity plays a …

Latest Quaternary Paleoclimate Reconstruction Utilizing Stable Isotopic And Trace Element Proxies In A Stalagmite From Culverson Creek Cave, West Virginia, Ashley Nicole Gilbert

University of Kentucky Master's Theses

A reconstruction of regional climate variability in southern West Virginia that spans the last glacial/interglacial transition is presented. Paleoclimate interpretations obtained from the 50-cm long stalagmite provide key insights regarding the timing, magnitude, and forcing mechanisms responsible for past climate variability. Stable isotopic (δ¹⁸O and δ¹³C) and trace element (Ba, Sr, Mg) signatures from samples contiguously milled along the growth-axis of a ²³⁰Th-dated stalagmite which grew between approximately 20 and 5 thousand years before present (kyr BP) provide critical constraints for above-cave mean annual temperature, seasonality of moisture mean annual precipitation, and potential vegetation shifts. …

Non-Portland Cement Activation Of Blast Furnace Slag, Anne Elizabeth Oberlink

University of Kentucky Master's Theses

The purpose of this project was to produce a “greener” cement from granulated ground blast furnace slag (GGBS) using non-Portland cement activation. By eventually developing “greener” cement, the ultimate goal of this research project would be to reduce the amount of Portland cement used in concrete, therefore reducing the amount of carbon dioxide emitted into the atmosphere during cement production.

This research studies the behavior of mineral binders that do not contain Portland cement but instead comprise GGBS activated by calcium compounds or fluidized bed combustion (FBC) bottom ash. The information described in this paper was collected from experiments including …

Hydraulic Geometry Relationships And Regional Curves For The Inner And Outer Bluegrass Regions Of Kentucky, Ruth Roseann Brockman

University of Kentucky Master's Theses

Hydraulic geometry relationships and regional curves are used in natural channel design to assist engineers, biologists, and fluvial geomorphologists in the efforts undertaken to ameliorate previous activities that have diminished, impaired or destroyed the structure and function of stream systems. Bankfull channel characteristics were assessed for 14 United States Geological Survey (USGS) gaged sites in the Inner Bluegrass and 15 USGS gaged sites in the Outer Bluegrass Regions of Kentucky. Hydraulic geometry relationships and regional curves were developed for the aforementioned regions.

Analysis of the regression relationships showed that bankfull discharge is a good explanatory variable for bankfull parameters such …