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High-Field 19.6 T 27Al Solid-State Mas Nmr Of In Vitro Aluminated Brain Tissue, Pamela L. Bryant, Walter J. Lukiw, Zhehong Gan, Randall W. Hall, Leslie G. Butler
High-Field 19.6 T 27Al Solid-State Mas Nmr Of In Vitro Aluminated Brain Tissue, Pamela L. Bryant, Walter J. Lukiw, Zhehong Gan, Randall W. Hall, Leslie G. Butler
Randall W. Hall
The combination of 27Al high-field solid-state NMR (19.6 T) with rapid spinning speeds (17.8 kHz) is used to acquire 27Al NMR spectra of total RNA human brain temporal lobe tissues exposed to 0.10 mM Al3+ (as AlCl3) and of human retinal pigment epithelial cells (ARPE-19), grown in 0.10 mM AlCl3. The spectra of these model systems show multiple Al3+ binding sites, good signal/noise ratios and apparent chemical shift dispersions. A single broad peak (−3 to 11 ppm) is seen for the aluminated ARPE-19 cells, consistent with reported solution-state NMR chemical shifts of Al-transferrin. The aluminated brain tissue has a considerably …