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Full-Text Articles in Physical Sciences and Mathematics
L-Arginine Uptake And Metabolism Following In Vivo Silica Exposure In Rat Lungs, Leif D. Nelin, Gary S. Krenz, Louis G. Chicoine, Christopher A. Dawson, Ralph M. Schapira
L-Arginine Uptake And Metabolism Following In Vivo Silica Exposure In Rat Lungs, Leif D. Nelin, Gary S. Krenz, Louis G. Chicoine, Christopher A. Dawson, Ralph M. Schapira
Mathematics, Statistics and Computer Science Faculty Research and Publications
Pulmonary inflammation increases nitric oxide (NO) production via inducible nitric oxide synthase (iNOS). This study was performed to determine some of the factors that affect the availability of the NOS substrate, L-arginine (L-arg), in the intact lung subjected to silica-induced inflammation. Nitrate production, as an index of NO production, was significantly greater in silica-exposed lungs (53.5 ± 12.1 nmol/90 min) compared with controls (22.5 ±5.1 nmol/90 min, P < 0.05). This was accompanied by greater (P< 0.0001) 90-min [3H]L-arg uptake (62 ± 3% control, 82 ± 1% silica), a significantly (P < 0.005) increased permeability-surface area product for L-arg(0.28 ± 0.05 ml/min control, 0.63 ± 0.07 ml/min silica), and asignificantly (P < 0.001) increased urea production (1.16 ± 0.08µmol/90 min control, 1.77 ± 0.06 µmol/90 min silica). There was no difference in eNOS protein between groups and eNOS mRNA was not detectable in either group, whereas silica exposure resulted in the appearance of both iNOS protein and mRNA. Silica exposure increased CAT-1 and CAT-2 mRNA ~ 8-fold compared with controls. We conclude that the increase in NO production in silica-exposed lungs was associated with increased L-arg uptake from the vasculature, presumably resulting from increased CAT-1 and CAT-2, and by increased L-arg metabolism via arginase.
Studying The Functional Genomics Of Stress Responses In Loblolly Pine With The Expresso Microarray Experiment Management System, Lenwood S. Heath, Naren Ramakrishnan, Ronald R. Sederoff, Ross W. Whetten, Boris I. Chevone, Craig Struble, Vincent Y. Jouenne, Dawei Chen, Leonel Van Zyl, Ruth Grene
Studying The Functional Genomics Of Stress Responses In Loblolly Pine With The Expresso Microarray Experiment Management System, Lenwood S. Heath, Naren Ramakrishnan, Ronald R. Sederoff, Ross W. Whetten, Boris I. Chevone, Craig Struble, Vincent Y. Jouenne, Dawei Chen, Leonel Van Zyl, Ruth Grene
Mathematics, Statistics and Computer Science Faculty Research and Publications
Conception, design, and implementation of cDNA microarray experiments present a variety of bioinformatics challenges for biologists and computational scientists. The multiple stages of data acquisition and analysis have motivated the design of Expresso, a system for microarray experiment management. Salient aspects of Expresso include support for clone replication and randomized placement; automatic gridding, extraction of expression data from each spot, and quality monitoring; flexible methods of combining data from individual spots into information about clones and functional categories; and the use of inductive logic programming for higher-level data analysis and mining. The development of Expresso is occurring in parallel with …