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Full-Text Articles in Physical Sciences and Mathematics
Wavelength-Specific Fluorescence Coefficients For Simulating Hyperspectral Reflectance Signatures Of Water, Charles R. Bostater, Jan Rebman
Wavelength-Specific Fluorescence Coefficients For Simulating Hyperspectral Reflectance Signatures Of Water, Charles R. Bostater, Jan Rebman
Ocean Engineering and Marine Sciences Faculty Publications
A model1'2 which describes the transfer of irradiant light in water is used to predict the fluorescence response ofthe water surface reflectance under solar induced or an artificial light source such as a laser. Formulations for the estimation of wavelength dependent fluorescent coefficients. The techniques allows the description ofa fluorescence reflectance response in deep and shallow waters with various bottom reflectance signatures such as submerged vegetation, corals and sand. Recent advances in the model are presented for obtaining wavelength dependent fluorescence spectrum responses from the solutions of the two flow equations following the procedures developed by Bostater1'2'3. Synthetic or modeled …
Near-Field Fluorescence Microscopy Based On Two-Photon Excitation With Metal Tips, Erik J. Sánchez, Lukas Novotny, X. Sunney Xie
Near-Field Fluorescence Microscopy Based On Two-Photon Excitation With Metal Tips, Erik J. Sánchez, Lukas Novotny, X. Sunney Xie
Physics Faculty Publications and Presentations
We present a new scheme for near-field fluorescence imaging using a metal tip illuminated with femtosecond laser pulses of proper polarization. The strongly enhanced electric field at the metal tip (ap15 nm end diameter) results in a localized excitation source for molecular fluorescence. Excitation of the sample via two-photon absorption provides good image contrast due to the quadratic intensity dependence. The spatial resolution is shown to be better than that of the conventional aperture technique. We used the technique to image fragments of photosynthetic membranes, as well as j-aggregates with spatial resolutions on the order of 20 nm.