Physical Sciences and Mathematics Commons^™

Isolation And Characterization Of A Crude Extracellular Phenol Oxidase From A Recirculation Bioreactor, Yun Tang

Theses

The study of phenol biodegradation has been performed using calcium alginate entrapped activated sludge in a recirculation reactor. An effective recirculation reactor system may be used on the laboratory scale to produce phenol oxidase, Phenol oxidase was isolated and concentrated from the degradation solution of phenol by ultrafiltration and freeze-drying respectively,

The effects of various concentrations of phenol, pH, temperature, ionic strength, cations, anions and 2- chlorophenol were studied on the oxidation reaction of enzyme. The optimal conditions of activated sludge phenol oxidase are [phenol] = 100 ppm; pH = 6.0; temperature 37°C; ionic strength equivalent to 0.1 M Na …