Physical Sciences and Mathematics Commons^™

Statistical Analysis Of Spectrophotometric Assays In The Presence Of Interference, Edward Gbur, Patti Landers, Roy Sharp

Conference on Applied Statistics in Agriculture

Indirect measurement of the amount of a specified component in a sample of a chemical compound can be accomplished by spectrophotometry. The underlying principle is Beer's Law, which states that, in a pure system, the amount of light absorbed by a chemical bond is linearly related to its concentration. In some mixtures it may not be possible to find a wavelength at which only the bond of interest absorbs light. Hence, the absorbance is composed of contributions from the bond of interest and one or more other (nuisance) bonds. Chemists refer to this situation as interference. In this paper we …