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Kinetics Of Reduction Of Cytochrome C Oxidase By Dithionite And The Effect Of Hydrogen Peroxide, M. Brunori, D. Bickar, J. Bonaventura, C. Bonaventura

Chemistry: Faculty Publications

The reduction of cytochrome c oxidase by dithionite was reinvestigated with a flow-flash technique and with varied enzyme preparations. Since cytochrome a3 may be defined as the heme in oxidase which can form a photolabile CO adduct in the reduced state, it is possible to follow the time course of cytochrome a3 reduction by monitoring the onset of photosensitivity. The onset of photosensitivity and the overall rate of heme reduction were compared for Yonetani and Hartzell-Beinert preparations of cytochrome c oxidase and for the enzyme isolated from blue marlin and hammer-head shark. For all of these preparations the faster phase …