Physical Sciences and Mathematics Commons^™

Fecal Pellet Production By North Atlantic Zooplankton, Michael Gibson

Undergraduate Honors Theses

Fecal pellet carbon (FPC) production by zooplankton is a significant component of the ocean’s biological carbon pump: the suite of biological processes that mediate export of carbon to the deep ocean, ultimately leading to the sequestration of atmospheric carbon dioxide in the ocean. In this study, mesozooplankton (zooplankton 0.2 mm to ~2 cm) were collected from the epipelagic zone in the temperate North Atlantic Ocean during day and night in May 2021. Zooplankton were live separated into five size fractions and incubated on board ship in natural surface seawater to measure fecal pellet production rate of the mixed mesozooplankton community. …

From Field To Shore: Policy Improvements To Better Protect Ecosystem And Human Health From Poultry Wastes In Virginia, Rachael A. King

Dissertations, Theses, and Masters Projects

Commercial poultry production is a large industry with economic importance in Virginia. However, mismanagement of manures and wastes generated from the poultry industry can be a serious threat to ecosystem and human health. Primarily, there is a concern about nutrient pollution related to runoff and infiltration of poultry wastes. High nutrient loading can cause groundwater contamination, eutrophication, and harmful algal blooms (HABs). Additional concerns include metals, antibiotics, and pesticides that can be found in poultry wastes. To determine existing threats to ecosystem and human health from the poultry industry, Virginia policies regulating the industry are reviewed and their strengths and …

Tracing Atlantic Sea Scallops Using Radio Frequency Identification (Rfid) Technology, Will Shoup

Dissertations, Theses, and Masters Projects

Traceable seafood can be linked back to its origin and method of catch. Improving the traceability of marine organisms involves establishing a transparent Chain of Custody (CoC) by collecting data at checkpoints throughout the supply chain, from ship to shore to store. This report explores the feasibility of integrating Radio Frequency Identification (RFID) technology into the United States Atlantic sea scallop (Placopecten magellanicus) fishery in order to improve traceability. This report serves as a forward-looking evaluation of RFID technology that is intended to inform interested stakeholders of its functionality and capabilities. It is not intended to serve as a management …

Impacts And Uncertainties Of Climate Change On The Chesapeake Bay, Kyle E. Hinson

Dissertations, Theses, and Masters Projects

Climate change impacts in the Chesapeake Bay will limit the efficacy of nutrient reduction efforts and decrease dissolved oxygen, but uncertainties associated with the magnitude of these effects remain. An understanding of underlying mechanisms that have driven recent warming trends will narrow uncertainties for future pathways of temperature change. Additionally, future simulations of climate impacts in the estuary are dependent on multiple different sources of uncertainty, many of which have not yet been fully evaluated. This dissertation used a three- dimensional coupled hydrodynamic-biogeochemical model to investigate recent warming trends as well as underlying uncertainties likely to influence regional projections of …

Climate Change And Conservation Of Milkweed: Evidence Of Extensive Admixture Between Common Milkweed And Poke Milkweed, Elizabeth Davies

Undergraduate Honors Theses

Global climate change can drive many changes in species interactions. One primary way it affects species is by changing climates, causing species to expand their ranges and allowing them to interact with species from whom they were previously isolated. In plants, new species interactions can result in hybridization – the creation of hybrid offspring between two separate species. This hybridization can increase gene flow between the species and lead to introgression, the transfer of genetic material from one species to another through hybrid backcrossing with the parent species. My thesis investigates hybridization in the model system Asclepias (milkweed) by analyzing …

Using Deep Learning With Satellite Imagery To Estimate Deforestation Rates, Maeve Naughton-Rockwell

Undergraduate Honors Theses

Previous studies have used Convolutional Neural Networks for regional detection of deforestation breaks. However, there is limited research into the capability of deep neural networks to identify sudden shifts in global forest cover from satellite imagery. Additionally, many deforestation detection models are trained on region specific data and need manual input thresholds. In this work, we develop a deep learning model to predict the percent of deforestation in a region between two points in time, trained on globally sourced data. Using the before and after satellite images of a deforestation event as inputs, we implemented a two input Convolutional Neural …

Using A Machine Learning Model To Predict Plant Inflorescences Based Upon Its Soil Microbiome, Luke Denoncourt

Undergraduate Honors Theses

The UN estimates that the global population could reach 9.7 billion by 2050 (United Nations). As a result, the amount of food required to feed humanity is thought to double by 2050 (Ray et al., 2012). Humanity must find a way to increase crop production without increasing fertilizer usage and eutrophication, which can be done using the soil microbiome. Using potted plants with soils inoculated with Pseudomonas alcaligenes, Pseudomonas denitrificans, Bacillus polymyxa, and Mycobacterium phlei, both the shoot and root growth of pea and cotton plants was significantly increased (Egamberdieva & Höflich, 2004). In this study, utilizing a random forest …

Modeling And Analyses Of Mechanisms Underlying Network Synaptic Dynamics In Two Neural Circuits, Linda Ma

Undergraduate Honors Theses

In systems neuroscience, circuit models of cortical structures can be used to deconstruct mechanisms responsible for spike patterns that generate a variety of behaviors observed in the brain. In particular, mathematical simulations of these circuits can replicate complex dynamical behaviors that mirror not only macroscopically patterns observed in the brain, but also a significant amount of experimentally characterized minutiae. These models are capable of analyzing neural mechanisms by explicitly deconstructing connectivities between populations of neurons in ways that tend to be empirically inaccessible. This work presents two such models; one in the rat somatosensory barrel cortex, responsible for processing sensory …

Analysis Of Edna To Assess Effects Of Water Quality On Freshwater Fungal Diversity In A Virginia Coastal Watershed, Lauren French

Undergraduate Honors Theses

Freshwater fungi comprise a phylogenetically and functionally diverse group which contributes to wide-ranging ecosystem processes in aquatic systems. Saprotrophic fungi convert detritus into nutrient-rich food sources for fish and invertebrates, whereas pathogenic and parasitic fungi can cause disease and population declines of other aquatic organisms. With their diverse and important roles, changes in freshwater fungal community structure may have far-reaching impacts on ecosystems. To understand how natural and anthropogenic stressors to freshwater systems impact fungal-mediated ecosystem processes, a greater understanding of the taxonomic and functional composition of freshwater fungal communities is needed. We assessed relationships among freshwater habitat types, water …

Honey As A Biomonitor For Air Pollutant Deposition In The Eastern United States Using Ion Chromatography And Scanning Electron Microscopy, Cole Cochran

Undergraduate Honors Theses

Anthropogenic activities generate metal, acid, and particulate air pollutants which negatively impact human and ecological health. In the United States, power plant, industrial, and vehicle emissions are leading causes of air pollution, however, the measurement of air pollution at high-resolution spatial regimes remains a challenge. Honey has emerged as a powerful biomonitoring tool to effectively quantify contaminants without the need for a large array of monitoring instruments. I hypothesized that honey could be used to effectively measure and map modern air pollutant spatiotemporal relationships over the Eastern U.S. Using ion chromatography with sulfate as an indicator for air pollution and …

The Role Of Zooplankton Community Composition In Fecal Pellet Carbon Production In The York River Estuary, Chesapeake Bay, Kristen Nicole Sharpe

Dissertations, Theses, and Masters Projects

The biological pump is a critical component of carbon transformation in aquatic ecosystems, but the role that zooplankton play in carbon production and vertical export is rarely studied in estuaries. Zooplankton produce carbon-rich fecal pellets which sink to depth and can fuel benthic community metabolism. The body size and taxonomic structure of the zooplankton community varies on interannual, seasonal, and diel time scales, and can lead to varying carbon production and export rates. We quantified fecal pellet carbon (FPC) production by the whole mesozooplankton community (> 200 µm) in the York River, a sub-estuary of Chesapeake Bay. Biomass and taxonomic …

Ecosystem Enriching And Efficient Solar Energy: Exploring The Effects Of Pollinator-Friendly Solar Facilities On Ecosystem Function And Solar Panel Efficiency, Jordan Martin

Dissertations, Theses, and Masters Projects

As the solar energy industry grows, many hundreds of thousands of acres of land will be transformed into solar panel facilities. With this large change in land use, there is the opportunity to promote biodiversity and support pollinators by using pollinator-friendly management practices at the solar facilities. This paper explores the ecological and economic effects of a pollinator-friendly solar facility compared to a turfgrass solar facility.

I hypothesized that a pollinator-friendly solar facility would be functionally equivalent in pollinator support and overall insect diversity to a pollinator-friendly non-solar field and that both sites would have far greater pollinator support and …

Diel, Seasonal, And Interannual Changes In Coastal Antarctic Zooplankton Community Composition And Trophic Ecology, John A. Conroy

Dissertations, Theses, and Masters Projects

Throughout the ocean, zooplankton transfer energy from primary producers to higher predators and transport carbon from surface waters to depth. The efficiency of these processes depends in part upon the taxonomic composition and trophic ecology of the zooplankton community. Zooplankton species abundance and distribution shifted over recent decades along the West Antarctic Peninsula during a period of rapid regional warming and sea-ice decline. Although conducted within the context of long-term change, this dissertation research focuses on zooplankton dynamics at finer temporal scales that have received less attention. I analyzed depth-stratified net samples to investigate zooplankton diel vertical migration during Antarctic …

Estuarine Microbiomes And Biogeochemistry: Impacts Of Spatiotemporal Variation, Algal Blooms, And Microplastics, Samantha Grace Fortin

Dissertations, Theses, and Masters Projects

Estuaries are biogeochemical hotspots connecting terrestrial and coastal ecosystems. Anthropogenic disturbances, including increased nitrogen loading and plastic pollution, may have significant impacts on estuarine carbon and nitrogen cycling by altering microbiome structure and functions. The overarching goal of this dissertation was to examine how microbiomes and their associated biogeochemical processes are influenced by natural variation and anthropogenic disturbances in the York River Estuary (YRE). In chapter 2, spatial and temporal variation in benthic microbiomes and the rates of denitrification, anaerobic ammonium oxidation (anammox), and dissimilatory nitrate reduction to ammonium (DNRA) were examined to determine biotic and abiotic drivers of nitrogen …

Three Centuries Of Vegetation Change In The William & Mary College Woods Reconstructed Using Phytoliths, Timothy Terlizzi

Undergraduate Honors Theses

The College Woods, west of William & Mary’s campus, consists of ~900 acres of protected southern mixed hardwood forest. The woods surround Lake Matoaka, a former millpond established in ~1700. Despite the rich history of the area, little is known about how the dominant vegetative landcover has shifted over the last 300 years. This study set out to quantify the modern vegetation within the College Woods via the phytolith assemblages within the soil and identify shifts in the assemblages since the creation of Lake Matoaka and whether these changes are distinct from the vegetation that existed in the area before …

Proteomic Analysis Of Mycobacteriophage Crimd, William Moeller

Undergraduate Honors Theses

Bacteriophages represent a large portion of the biomatter on our planet, and many of them have yet to be fully characterized. Here we discuss the proteomic analysis of a particular Bacteriophage, Mycobacteriophage CrimD. This phage was discovered on the Campus of William & Mary and has had its genome characterized. We took the next logical step of proteomic analysis.

In our analyses we made use high pressure liquid chromatography paired with linear ion trap mass spectrometry to analyze the proteome of CrimD at specific time points after the infection of its host, Mycobacterium smegmatis. Additionally, we used nanospray ionization with …

Improving Observation, Assessment, And Management Of Atlantic Coastal Sharks, Cassidy Dawn Peterson

Dissertations, Theses, and Masters Projects

Coastal sharks represent a group of stocks for which observation, assessment, and management are particularly challenging. Large distributional ranges, complex migratory behavior, low economic value, and relatively few observations in fishery independent surveys hinder relative abundance estimation. Assessing stock status of coastal sharks is encumbered by limited data availability, data quality, and knowledge of life history strategy. Further, coastal sharks are challenging to manage due to their slow intrinsic population growth rates, competing stakeholder interests, history of overexploitation, and in some cases, subjection to international exploitation. This dissertation aimed to improve the capacity to observe relative abundance of coastal sharks. …

Quantifying The Increased Resiliency Of Chesapeake Bay Hypoxia To Environmental Conditions: A Benefit Of Nutrient Reductions, Luke Thomas Frankel

Dissertations, Theses, and Masters Projects

Seasonal hypoxia is a characteristic feature of the Chesapeake Bay as a result of anthropogenic eutrophication from agriculture and urban development throughout the watershed. Although in recent years coordinated management efforts have successfully reduced the flux of nutrients into the Bay, the overall goal of sufficient oxygen concentrations below the pycnocline for living resources remains unfulfilled. This was particularly apparent in 2018 and 2019 when the volume of hypoxic water exceeded the long-term (35-year) average due to anomalously high riverine discharge. To quantify the impact of watershed nutrient reductions, conventional statistical methods were employed in concert with a 3-D numerical …

Recruitment And Post-Settlement Mortality Of The Soft-Shell Clam, Mya Arenaria, Shantelle Landry

Dissertations, Theses, and Masters Projects

The soft-shell clam, Mya arenaria, is a benthic, filter-feeding, infaunal clam typically found in intertidal and shallow subtidal waters. Chesapeake Bay stocks of M. arenaria have been depleted since the 1960s due to various factors including predation, temperature, low recruitment, habitat loss, disease mortalities, and commercial harvest. As an important prey item for many commercial species, low abundances of these clams are mostly the result of the voracious appetite of the blue crab, Callinectes sapidus. In addition to predation, summer water temperatures in the Chesapeake Bay are likely driving the low abundances of M. arenaria, as water temperatures commonly surpass …

Evaluating The Use Of Acoustic Warning Signals To Reduce Avian Collision Risk, Robin Grace Thady

Dissertations, Theses, and Masters Projects

Collisions with human-made structures are responsible for billions of bird deaths each year, resulting in ecological damage as well as regulatory and financial burdens to many industries. Acoustic warning signals can alert birds to obstacles in their flight paths in order to mitigate collisions, but these signals should be tailored to the sensory ecology of birds in flight. The effectiveness of various acoustic warning signals likely depends on the influence of background noise and the relative ability of various sound types to propagate within a landscape. I evaluated the ability of four sound signals to elicit collision-avoidant flight behaviors from …

Endangered Species Act: Quantifying Threats Impacting Listing, Delaney Costante

Dissertations, Theses, and Masters Projects

With species increasingly becoming imperiled due to anthropogenic activities, conservation practitioners are tasked with determining conservation priorities in order to make the best use of limited resources. The United States’ Endangered Species Act (ESA) has two listing statuses into which imperiled species are placed to receive protections: Threatened or Endangered. In the first chapter, our objective was to identify differences between Threatened and Endangered species beyond what is outlined in their ESA definitions. To our knowledge, this is the first study to compare listing status for species protected by the ESA on the basis of types and number of threats …

Dealing With Many Species: Improving Methodology For Forming And Assessing Species Complexes, Kristen Omori

Dissertations, Theses, and Masters Projects

In the United States, the Magnuson-Stevens Reauthorization Act mandates that all federally fished species must have catch limits, which can be challenging for data-limited species. One approach is to assess and manage a group of species with similar life history characteristics, vulnerability to the fishery, and overlapping geographic distributions in a single management unit, or a complex (i.e., stock or species complex). Using the Gulf of Alaska (GOA) Other Rockfish complex as a case study, the main goals of this dissertation are five-fold: 1) review species complexes in the United States; 2) compare multivariate techniques for assigning species to complexes; …

Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts

Dissertations, Theses, and Masters Projects

Cultural eutrophication, the overproduction of phytoplankton biomass in response to increased nutrient inputs directly associated with human activities, is a major threat to the health of Chesapeake Bay. Strict regulations, which require a reduction in nutrient loading from all sources, have been a key component to restoration efforts. Water reclamation facilities (WRFs), which discharge effluent containing nitrogen (N) and other nutrients into receiving waters, have implemented upgrades in an effort to comply with regulations. These improvements have decreased the concentration of highly labile dissolved inorganic N (DIN), leaving behind significant concentrations of dissolved organic N (DON) whose bioavailability, and therefore …

Reducing Avian Collisions With Human-Made Structures: A Sensory Ecology Approach To Open-Air Settings, Timothy James Boycott

Dissertations, Theses, and Masters Projects

Billions of birds fatally collide with human-made structures each year. These mortalities have impacts on species of conservation concern and potentially on avian populations as a whole. This source of human-wildlife conflict also places economic and operational constraints on various human industries. Furthermore, with continued increases in urbanization, the rate and extent of collisions continues to increase. Efforts to reduce collisions have largely centered on making structures more visible to birds but have been met with limited success. Currently, there is a call for solutions to be tailored to both the environmental context of hazardous structures and to the sensory …

Investigating Economic Costs Of Derelict Blue Crab Callinectes Sapidus Pots And Preferred Mitigation Solutions In The Chesapeake Bay, James Delbene

Dissertations, Theses, and Masters Projects

Derelict fishing gear, particularly pots or traps, occupy waters worldwide and cause negative ecological and economic impacts. Derelict pots persist throughout Chesapeake Bay, the largest estuary in the U.S., that supports a valuable commercial fishery for the blue crab Callinectes sapidus. Chesapeake Bay is responsible for 30-40% of U.S. commercial blue crab harvests. Yet, few studies have quantified the impacts of derelict pots on harvest or the perceptions of commercial fishers on derelict pot mitigation activities in this predominantly pot fishery. This thesis examined the impacts of derelict pots on harvest in a field experiment and worked with commercial fishers …

Environmental Controls On Pteropod Ecology And Physiology Along The Western Antarctic Peninsula, Patricia Susan Thibodeau

Dissertations, Theses, and Masters Projects

Pteropods (pelagic snails) are ubiquitous zooplankton in the Southern Ocean and abundant along the Western Antarctic Peninsula (WAP), one of the most rapidly warming regions on the planet. They are important prey for higher trophic levels, grazers of phytoplankton, and contribute to particulate organic and inorganic carbon export. Pteropods are heralded as bioindicators of ecosystem health due to the vulnerability of their aragonitic shells under ocean acidification conditions, which could greatly affect their abundances in the future. Despite their importance within Antarctic food webs, few studies have analyzed the effects of climate change on pteropod physiology and biogeography in the …

Partitioning Of Hydrophobic Organic Contaminants And Microbial Communities On Microplastics, Kelley Ann Uhlig

Dissertations, Theses, and Masters Projects

Microplastic contamination of aquatic environments has only recently caught the attention of scientists, regulators and the public. Microplastics are typically more recalcitrant than naturally occurring polymers and so have the potential to cause a range of issues, including increased exposure of marine life to chemical contaminants sorbed to or leached from microplastics, negative impacts due to ingestion of microplastics by biota, and the potential to carry and transport pathogenic and invasive species long distances. Bio-based, bio-degradable polymers have begun to gain market share as an alternative to traditional petrochemical-based plastics, but not much is known about their impacts in marine …

Human Dimensions Of A Participatory, Collaborative Modeling Process - Oysterfutures, Taylor Dawn Goelz

Dissertations, Theses, and Masters Projects

Participatory, collaborative modeling processes represent a unique decision-making technique within natural resources management that allows for the combination of stakeholder involvement with the analytical and predictive power of scientific models. The continued use of participatory modeling within decision-making processes depends in part upon the willingness of stakeholders to participate. Continued participation of stakeholders is key to the persistence and overall success of these processes, and yet limited information exists concerning the impacts of these processes on participants. The consideration of human dimensions advances our understanding of the design and function of participatory modeling processes, including their ability to create consensus …

Applying The Concept Of Thresholds In Ecotoxicology With Focus On The Joint Effects Of Habitat Fragmentation And Contamination, Marcos Krull

Dissertations, Theses, and Masters Projects

A threshold can be defined as the point where small changes in an environmental driver produce an abrupt change within a biological system. These changes can occur at different levels of organization, from organisms to ecosystems. Although thresholds seem to be receiving more attention by ecotoxicologist, not much is known about how contaminants cause or affect thresholds at the landscape level, such as habitat fragmentation thresholds. Habitat fragmentation thresholds can occur due to rapid changes in the landscape structure after a certain amount of habitat is lost, which can cause abrupt effects on the movement of organisms, population abundance and …

Assessing The Impact Of Land Use And Climate Change On Streamflow And Nutrient Delivery To The New River Estuary, Nc, Shanna Williamson

Dissertations, Theses, and Masters Projects

Freshwater inflow influences numerous physical, chemical, and biological characteristics of estuaries. The influx of freshwater to an estuary typically serves as an important source of allochthonous material from which primary producers derive their energy and transfer this energy to higher trophic levels. Any changes to freshwater flow subsequently impacts nutrient delivery and indirectly impacts organisms across multiple trophic levels. Anthropogenic changes to coastal land use and climate both act to threaten the integrity of estuarine systems by influencing freshwater inflow and dissolved nutrient input. Watershed loading models such as the Regional Nutrient Management (ReNuMa) model offer the ability to estimate …