Physical Sciences and Mathematics Commons^™

Refinement Of Biomarker Pentosidine Methodology For Use On Aging Birds, Crissa K. Cooey, Jesse A. Fallon, Michael L. Avery, James T. Anderson, Elizabeth A. Falkenstein, Hillar Klandorf

USDA Wildlife Services: Staff Publications

There is no reliable method for determining age for most species of long-lived birds. Recent success using the skin chemical pentosidine as a biomarker has shown promise as an aging tool for birds. Pentosidine levels have been determined only from the breast tissue of carcasses, and we sought to refine the procedure with respect to biopsy size and location for safe and effective use on living birds. We compared pentosidine concentrations in 4 skin-size samples (4, 6, 8, and 20-mm diameter biopsies) from the breast of black vulture (Coragyps atratus) carcasses. We also compared pentosidine levels from breast …

Evaluation Of Rhodamine B As A Biomarker For Raccoons, Tricia L. Fry, Todd C. Atwood, Mike R. Dunbar

USDA Wildlife Services: Staff Publications

The USDA/APHIS/Wildlife Services (WS) oral rabies vaccination program uses tetracycline, a broad-spectrum antibiotic and relatively reliable biomarker, to quantify vaccinebait uptake by raccoons (Procyon lotor). However, obtaining samples (e.g., bone or teeth) to assess tetracycline uptake is highly invasive, and sample preparation can be expensive. By contrast, rhodamine B, a commercially available dye, is absorbed systemically in growing tissues, including hair and whiskers, and can be observed under ultraviolet (UV) light as fluorescent orange bands. Our goal was to evaluate whether rhodamine B can be used as a biomarker to monitor bait uptake by raccoons. We began by …

Reducing The Risk Of Human Exposure To Wildlife Diseases, Tyler A. Campbell

USDA Wildlife Services: Staff Publications

As a professional biologist working for a government agency, I am required to carry a card alerting medical personnel that I may have been exposed to certain zoonotic diseases (animal diseases that can infect man) not routinely considered in differential diagnosis. Some of these pathogens are obscure and seldom heard of, such as monkeypox and Q fever; others more commonly make headlines, such as influenza, West Nile virus, Lyme disease, and Hantavirus Pulmonary Syndrome. Hunters, ranchers, and biologists who work with wildlife have an increased risk of acquiring these diseases directly from animal hosts or their parasites. Here are some …

On A Quest For Safer Skies: Managing The Growing Threat Of Wildlife Hazards To Aviation, Gail Keirn, Jonathon Cepek, Bradley Blackwell, Travis L. Devaul

USDA Wildlife Services: Staff Publications

The images remain indelible: On a chilly January day in 2009, a U.S. Airways Airbus A320 departed from New York’s LaGuardia Airport. About five miles out, flying at 2,000 feet, it collided with a flock of Canada geese (Branta canadensis), severely damaging the plane’s engines. Within minutes of the collision the pilot safely conducted an emergency landing on the Hudson River. All 155 passengers survived.

Increased Abundance And First Breeding Record Of The Neotropic Cormorant (Phalacrocorax Brasilianus) On The Alluvial Plain Of Mississippi, Katie C. Hanson, Travis L. Devault, Stephen J. Dinsmore

USDA Wildlife Services: Staff Publications

Phalacrocorax brasilianus (Neotropic Cormorant) has been observed with increasing frequency in the alluvial plain (Delta region) of Mississippi. In the past 6 years, 22 individuals have been observed in 20 separate sightings during spring and summer. These sightings have occurred at breeding colonies of other colonial waterbirds and commercial aquaculture facilities of Ictalurus punctatus (Channel Catfish). Two sexually mature Neotropic Cormorants have been collected at a colonial waterbird breeding colony near the Mississippi River in the western Delta region among flocks of Phalacrocorax auritus (Double-crested Cormorants). Twice during the summer of 2008, confirmed nesting of Neotropic Cormorants were documented in …

A Review Of Existing And Potential New World And Australasian Vertebrate Pesticides With A Rationale For Linking Use Patterns To Registration Requirements, Charles T. Eason, Kathleen A. Fagerstone, John D. Eisemann, Simon Humphrys, Jeanette R. O’Hare, Steven J. Lapidge

USDA Wildlife Services: Staff Publications

Registration is a necessarily sophisticated evaluation process applied to vertebrate pesticide products. Although conducted to minimize any potential impacts upon public health, the environment and food production, the all-encompassing process of registration can stifle innovation. Vertebrate pesticides are rarely used to control pest animals in food crops. In contrast to agrochemicals, relatively small amounts of vertebrate pesticides are used (50.1%), usually in solid or paste baits, and generally by discrete application methods rather than by broad-scale spray applications. We present a hierarchy or sliding scale of typical data requirements relative to application techniques, to help clarify an evolving science-based approach …

Immunocontraception In Male Feral Swine Treated With A Recombinant Gonadotropin-Releasing Hormone Vaccine, Tyler A. Campbell, Michelle R. Garcia, Lowell A. Miller, Martha A. Ramirez, David B. Long, Jean-Baptiste Marchand, Fergal Hill

USDA Wildlife Services: Staff Publications

Objective: To determine if a recombinant gonadotropin-releasing hormone (GnRH) vaccine is a potential immunocontraceptive agent for juvenile male feral swine.

Results: As expected, a single dose of GonaCon vaccine reduced testes mass, serum testosterone, and percent normal tubules, and restricted sperm development at each stage. These reductions in reproductive development were associated with elevated GnRH antibodies. The single injection of rGnRH was not as effective in reducing these reproductive parameters; however, the two-dose injection of rGnRH was as effective as the single injection of GonaCon.

Why Don’T Rats And Mice Vomit? A Behavioral And Anatomical Investigation, C. C. Horn, National Wildlife Res. Ctr, G.R. Gathright, B. Yates, P.L. Andrews

USDA Wildlife Services: Staff Publications

Laboratory rats and mice are known to lack a vomiting response and the dimensions of the abdominal esophagus might be an important constraint (Andrews, 1995, Physiol. Zool.). However, a broad evaluation of Rodentia is lacking. Here we determined the behavioral responses and esophageal and diaphragm anatomy from 4 of the 5 Suborders.Weused prototypical emetic agents, apomorphine (s.c.), veratrine (s.c.), and copper sulfate (i.g.), which are thought to produce emesis by action on the area postrema, nodose ganglia, and vagal afferent fibers, respectively. None of the rodents, including nutria (Myocastor coypus), beavers (Castor canadensis), mountain beavers …

Avian Piscivores As Vectors For Myxobolus Cerebralis In The Greater Yellowstone Ecosystem, Todd M. Koel, Billie L. Kerans, Scott C. Barras, Katie C. Hanson, John S. Wood

USDA Wildlife Services: Staff Publications

Myxobolus cerebralis, the cause of whirling disease in salmonids, has dispersed to waters in 25 states within the USA, often by an unknown vector. Its incidence in Yellowstone cutthroat trout Oncorhynchus clarkii bouvieri within the highly protected environment of Yellowstone Lake, Yellowstone National Park, is a prime example. Given the local abundances of piscivorous birds, we sought to clarify their potential role in the dissemination of M. cerebralis. Six individuals from each of three bird species (American white pelican Pelecanus erythrorhynchos, double-crested cormorant Phalacrocorax auritus, and great blue heron Ardea herodias) were fed known-infected or …

Effects Of Aquaculture On Migration And Movement Patterns Of Double-Crested Cormorants, D. Tommy King, Bradley F. Blackwell, Brian S. Dorr

USDA Wildlife Services: Staff Publications

We analyzed 10,620 recovery records for double-crested cormorants (Phalacrocorax auritus) banded as nestlings from 1923 to 2006 to determine the population’s age structure, migration routes, dispersal patterns, and the possible influence of the expansion of the aquaculture industry in the southeastern United States on these population characteristics. Ninety-nine percent of the birds were banded during June to August, and 78% were banded as pre-fl edged birds. Cormorants banded in the interior region of the United States comprised 91% of all birds banded from 1955 to 2006; these birds wintered primarily in the Lower Mississippi Valley and the northern …

Eradicating Rats On Lehua Island, Hawaii, With The Help Of Gis And Gps, Justin W. Fischer, Peter Dunlevy

USDA Wildlife Services: Staff Publications

Lehua Island is an uninhabited, 290-acre crescent-shaped volcanic cone located approximately 150 miles north-northwest of Honolulu, Hawaii, or approximately 20 miles west of the island of Kauai. Lehua is a state-designated seabird sanctuary managed by the Hawaii Department of Land and Natural Resources (HIDLNR) and federally owned by the U.S. Coast Guard. Renowned for its diversity of nesting seabirds, it is home to at least 17 recorded species of seabirds, including, but not limited to, colonies of Laysan and black-footed albatross, red-footed and brown boobies, black noddies, and Newell's shearwaters. Lehua is also home to several species of native coastal …

Visitation To Cottonseed Storage Sites By Feral Swine And Evidence Of Gossypol Exposure, Tyler A. Campbell, Sarah Bullock, David B. Long, David G. Hewitt, Michael Dowd

USDA Wildlife Services: Staff Publications

Texas ranks first in U.S. cotton production, and southern Texas is a major region of production within the state. Within Kleberg County, for example, approximately 16,147 ha are planted in cotton annually, yielding approximately 68,200 bales, or 15,467 metric tons, of cotton (U.S. Department of Agriculture [USDA] 2009). Cotton producers have discovered new uses for cotton ginned by-products, such as hydro-mulch (Holt et al. 2005) used as a protein supplement for range livestock (DelCurto et al. 2000) and white-tailed deer (Odocoileus virginianus; Cooper 2006). Because of this, much of the materials are temporarily stored for later use.

European Starling Preferences For Bait Substrates Used In Drc-1339 Applications, H. Jeffrey Usda-Aphis-Wildlife Services, George M. Linz, Scott F. Beckerman, Anthony Duffiney, Thomas Halstead

USDA Wildlife Services: Staff Publications

Additional bait substrates for the avicide, DRC-1339 Concentrate (3-chloro-4- methylaniline hydrochloride), could provide USDA/Wildlife Services with more flexibility when managing nuisance populations of European starlings (Sturnus vulgaris) at livestock facilities. From January 11 to 21, 2008, we conducted 11 2-choice preference tests with 6 bait types at a feedlot in central Kansas. The baits included cracked corn mixed with lard (2 concentrations), 2 forms of distiller’s grain (wet powder and pellets), 2 types of livestock feed (calf-starter pellet and sweet-feed mix), and a custom-produced poultry pellet (carrier pellet) made by USDA specifically for baiting starlings. We evaluated bait …

Livestock Protection Dogs In The 21st Century: Is An Ancient Tool Relevant To Modern Conservation Challenges?, Thomas M. Gehring, Kurt C. Vercauteren, Jean-Marc Landry

USDA Wildlife Services: Staff Publications

Europe and North America share a similar history in the extirpation and subsequent recovery of large carnivore and ungulate species. Both continents face challenges and opportunities for managing human-wildlife conflict at the junction of livestock production and wildlife conservation. Predation of livestock and disease transmission between wildlife and livestock is an ongoing and escalating worldwide issue. In order to manage this conflict, producers need effective tools, and they have used livestock protection dogs (LPDs) for reducing predation for well over 2000 years. We review the history of the use of LPDs, including the loss of information on their use and …

Eradicating Rats On Lehua Island, Hawaii, With The Help Of Gis And Gps, Justin W. Fischer, Peter Dunlevy

USDA Wildlife Services: Staff Publications

Highlights

• Wildlife Services used GIS and GPS to document and track bait distribution during each bait drop.
• GIS and GPS were critical in making this eradication project effective and environmentally safe.
• Use of the technologies ensured the coverage necessary for the project's goals.

Field Testing Class Iiib Handheld Lasers To Disperse Roosting Blackbirds, H. Jeffrey Homan, Anthony Slowik, Bradley F. Blackwell, George M. Linz

USDA Wildlife Services: Staff Publications

During late summer and fall, a hectare of cattail in a cattail-dominated wetland can hold about 1500 blackbirds. Thus, even small wetlands of 5-1 0 hectares that become roosts can cause significant problems for sunflower producers. USDA Wildlife Services has a cattail management program in North Dakota and South Dakota that helps sunflower producers eliminate cattail vegetation near susceptible fields. However, cattail-reduction treatments (which typically occur in August or September) do not break the cattail stalks, and the roosting substrate remains in place until the following year. Moreover, the sites selected for treatment are generally signed up at least one-year …

Assessment Of Bird Damage To Sunflower And Corn In North Dakota, Megan Klosterman, George M. Linz, Tony Slowik, William Bleier

USDA Wildlife Services: Staff Publications

North Dakota is the top sunflower producer in the United States, annually harvesting about 1 million acres (404,686 ha). The red-winged blackbird (RWBL), common grackle (COGR), and yellow-headed blackbird (YHBL) cause significant damage to these crops. Peer et al. (2003) estimated bird damage to sunflower at $5-10 million annually. Recently, corn has become a major crop within the state with 69,793,704.56 hectares planted in 2008 and 2009.
In the past 40 years, stable YHBL and increased COGR populations in the Prairie Pothole Region (PPR) may have lead to greater losses. While RWBL populations have declined slightly, populations in North Dakota …

Evaluation Of Large, Mobile, Decoy Traps For Managing Blackbird Damage To Ripening Sunflower, George M. Linz, Anthony Slowik, H. Jeffrey Homan, Robert Byrd

USDA Wildlife Services: Staff Publications

An estimated 75 million blackbirds migrate through the sunflower growing regions of the Great Plains and can cause severe damage to ripening sunflower and corn. If bird damage can be reduced or kept at levels <10% significant profits can be made. USDA Wildlife Services (WS) has developed some tools to help producers defend their fields against blackbird damage.

Eliminating problem roosts by applying glyphosate herbicide to dense stands of cattail in wetlands favored by blackbirds has been used successfully and has been a well received program. However, WS only has sufficient funds to treat <1 % of the estimated 547,000 acres of cattails in North Dakota.

Assessment Of Woven Wire For Reducing Predation On Red-Winged Blackbird Nests, Anthony A. Slowik, George M. Linz, H. Jeffrey Homan

USDA Wildlife Services: Staff Publications

Red-winged blackbirds (RWBL) are a polygynous species, and disruptions to either territorial behavior or reproductive fitness of the males has potential to lower annual productivity of several females. A reduction in the number of fledglings produced per territory could ultimately result in lower damage to grain crops, at least on a local scale. It is thus feasible that socially acceptable nonlethal methods, including reproductive inhibition through either physical or chemical means, may accomplish a reduction in crop damage.

Scientists at the USDA, Wildlife Services' National Wildlife Research Center have shown that reproductive inhibition is an effective method for reducing local …

An Evaluation Of Three Statistical Methods Used To Model Resource Selection, David M. Baasch, Andrew J. Tyre, Joshua J. Millspaugh, Scott E. Hygnstrom, Kurt C. Vercauteren

USDA Wildlife Services: Staff Publications

The performance of statistical methods for modeling resource selection by animals is difficult to evaluate with field data because true selection patterns are unknown. Simulated data based on a known probability distribution, though, can be used to evaluate statistical methods. Models should estimate true selection patterns if they are to be useful in analyzing and interpreting field data. We used simulation techniques to evaluate the effectiveness of three statistical methods used in modeling resource selection. We generated 25 use locations per animal and included 10, 20, 40, or 80 animals in samples of use locations. To simulate species of different …

Comparison Of Endocrine Response To Stress Between Captive-Raised And Wild-Caught Bighorn Sheep, Sarah Coburn, Mo Salman, Jack Rhyan, Thomas Keefe, Matthew Mccollum, Keith Aune, Terry Spraker, Lowell Miller

USDA Wildlife Services: Staff Publications

Stress hormones in Rocky Mountain bighorn sheep (Ovis canadensis canadensis), produced in response to environmental changes, road development, or high population density, may impact their immune systems to a threshold level that predisposes them to periodic, large-scale mortality. We compared the stress response to a novel environmental situation and repeated handling between bighorn sheep born and raised in captivity (CR) and bighorn sheep born in the wild (WC) and brought into captivity. We measured plasma epinephrine, norepinephrine, cortisol, and fecal glucocorticoid metabolites (FGM). Three weeks after each group’s arrival we used a one-time drop-net event to elicit an …

The Effect Of Enclosure Type On The Behavior And Heart Rate Of Captive Coyotes, Stacey P. Brummer, Eric M. Gese, John A. Shivik

USDA Wildlife Services: Staff Publications

The potential for captivity to elicit changes in animal behavior and physiology is well known. Recent research on captive populations has examined the effect of feeding protocols, enclosure types, and enrichment programs on indices of stress and displays of species-typical behaviors. We investigated the impact of enclosure type upon captive coyotes (Canis latrans) by examining differences in coyote behavior and heart rate, among 3.3m² kennels (K), 65.5m² small pens (S), and 1000m² large pens (L). Time budgets and repertoire of species-specific behaviors were compared among each enclosure type and to a sample of wild (W) …

Evaluation Of Harassment Of Migrating Double-Crested Cormorants To Limit Depredation On Selected Sport Fisheries In Michigan, Brian S. Dorr, Ashley Moerke, Michael Bur, Chuck Bassett, Tony Aderman, Dan Traynor, Russell D. Singleton, Peter H. Butchko, Jimmy D. Taylor Ii

USDA Wildlife Services: Staff Publications

Diverse management techniques have been used to mitigate conflicts between humans and double-crested cormorants (Phalacrocorax auritus) including harassment methods supplemented by lethal take. In this study we evaluated impacts or programs to harass spring migrating cormorants on the walleye (Sander vitreus) fishery in Brevoort Lake and the yellow perch (Perca flavescens ) and walleye fisheries at Drummond Island. Cormorant foraging declined significantly (p < 0.05) at both locations subsequent to initiation of harassment programs. Overall harassment deteired 90% of cormorant foraging attempts while taking less than 6% lethally on average at each site. Yellow perch were a predominate prey item in number and biomass at both locations. Walleye made up a small proportion of the diet at both locations. However, both walleye and yellow perch abundance increased significantly (p<0.05) at Drummond Island. Walleye abundance at age 3 increased to record levels in 2008 following 3 years of cormorant management at Brevoort Lake. The estimated cormorant consumption of age 1 walleye in the absence of management at Brevoort Lake during 2005 would account for 55% of the record 2006 age 1 walleye population. These results support the hypothesis, that cormorant predation on spawning aggregations of sportfish was a significant mortality factor and cormorant management reduced sportfish mortality and increased abundance at both locations. Continuation of harassment programs and .fishery assessments will determine whether improvement of targeted sport fisheries through control of spring migrating cormorants is sustainable.

Management Effects On Breeding And Foraging Numbers And Movements Of Double-Crested Cormorants In The Les Cheneaux Islands, Lake Huron, Michigan, Brian S. Dorr, Tony Aderrnan, Peter H. Butchko, Scott C. Barras

USDA Wildlife Services: Staff Publications

The yellow perch fishery of the Les Cheneaux Islands (LCl) region of Lake Huron, MI suffered a collapse in 2000, attributed in part to the increase of double-crested cormorants (Phalocrocorax auritus) in the region. A management program involving egg-oiling and lethal culling was initiated in 2004 to reduce cormorant foraging on yellow perch in the LC1. Counts of cormorant nests, nests oiled, cormorants culled, and aerial counts and telemetry surveys were used to evaluate management. Management contributed to a 74% reduction of cormorants on breeding colonies from 2004 to 2007. Cormorants used the LC1 area significantly more (P<0.05) than surrounding areas. Aerial counts of foraging cormorants declined significantly (P<0.05) over the entire survey area but not within the LCl proper. However, aerial counts of cormorants in the LCI were five-fold less than cormorant counts in the same area in 1995. Reduced cormorant numbers were attributed in part to the elimination of cormorant nesting on a large colony due to the introduction of raccoons. Although the numbers of cormorants using the LC1 did not decline, flocks were significantly smaller and more dispersed after management began. The reduced number of cormorants from 1995 levels and more dispersed foraging likely reduced predation on fish stocks including yellow perch in the LC1. Our findings indicate that the relationship between reduction in cormorant breeding numbers and reduced cormorant foraging in a given area is complex and may be influenced by density dependent factors such as intraspecific competition and quality of the forage base.

Acetaminophen As An Oral Toxicant For Nile Monitor Lizards (Varanus Niloticus) And Burmese Pythons (Python Molurus Bivittatus), Richard E. Mauldin, Peter J. Savarie

USDA Wildlife Services: Staff Publications

Context. Invasive species are a growing global problem. Biological invasions can result in numerous harmful impacts on local ecologies, and non-native herpetofauna are frequently ignored. Nile monitor lizards (Varanus niloticus) and Burmese pythons (Python molurus bivittatus, recently reassessed as Python bivittatus bivittatus), have become established in southern Florida. Both are large, semi-aquatic predators that pose serious threats to a variety of threatened and endangered species, as well as to the unique ecology of the area.

Aims. Acetaminophen (CAS#103-90-2), a lethal oral toxicant for the invasive brown treesnake (Boiga irregularis) on Guam, was investigated …

The Earliest House Sparrow Introductions To North America, Michael P. Moulton, Wendell P. Cropper Jr., Michael L. Avery, Linda E. Moulton

USDA Wildlife Services: Staff Publications

Several authors have argued that three separate introductions of roughly 100 individuals were required initially to establish the House Sparrow (Passer domesticus) in the Brooklyn, New York area. We argue that these claims are in error and that the actual record suggests that it is likely the initial introduction of just 16 birds in 1851 was all that was required to establish the species in New York. We further suggest that a similar level of scrutiny of historical records will reveal more examples of misinterpretations and errors, casting doubt on the validity of studies that claim propagule pressure …

Fine-Scale Genetic And Social Structuring In A Central Appalachian White-Tailed Deer Herd, Brad F. Miller, Randy W. Deyoung, Tyler A. Campbell, Benjamin R. Laseter, W. Mark Ford, Karl V. Miller

USDA Wildlife Services: Staff Publications

Spatial genetic structure in white-tailed deer (Odocoileus virginianus) has been examined at regional scales, but genetic markers with the resolution to detect fine-scale patterns have appeared only recently. We used a panel of microsatellite DNA markers, radiotelemetry data, and visual observations of marked deer to study fine-scale social and genetic structure in a high-density population of white-tailed deer (12–20 deer/km²). We collected genetic data on 229 adult females, 102 of which were assigned to 28 social groups. Our results were consistent with the conceptual model of white-tailed deer social structure, where philopatric females form social groups …

The Versatility Of Graded Acoustic Measures In Classification Of Predation Threats By The Tufted Titmouse Baeolophus Bicolor: Exploring A Mixed Framework For Threat Communication, Kathryn E. Sieving, Stacia A. Hetrick, Michael L. Avery

USDA Wildlife Services: Staff Publications

Many mammal and bird species respond to predator encounters with alarm vocalizations that generate risk-appropriate responses in listeners. Two conceptual frameworks are typically applied to the information encoded in alarm calls and to associated anti-predator behaviors. ‘Functionally referential’ alarm systems encode nominal classes or categories of risk in distinct call types that refer to distinct predation-risk situations. ‘Risk-based’ alarms encode graded or ranked threat-levels by varying the production patterns of the same call types as the urgency of predation threat changes. Recent work suggests that viewing alarm-response interactions as either referential or risk-based may oversimplify how animals use information in …

In Memory William B. Jackson 1926-2010, Michael W. Fall

USDA Wildlife Services: Staff Publications

DR. WILLIAM B. JACKSON of Chicago, Illinois, passed away July 15, 2010. He was a scientist, teacher, husband, father, grandfather, and friend. Over the years, he served as an advisor and mentor to many people who found their way to his classes or offices at Bowling Green State University (BGSU).

He was born in Milwaukee, Wisconsin, September 10, 1926, and spent many hours collecting insects and watching birds, becoming president of his high school nature club and an Eagle Scout. He earned B.A. and M.A. degrees from the University of Wisconsin, and his Sc.D. in vertebrate ecology from Johns Hopkins …

Response Of Captive Skunks To Microencapsulated Tetracycline, Brandon S. Schmit, Thomas M. Primus, Jerome C. Hurley, Dennis J. Kohler, Shawna F. Graves

USDA Wildlife Services: Staff Publications

A captive striped skunk (Mephitis mephitis) study was conducted between February and June 2004 at the United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services National Wildlife Research Center, Fort Collins, Colorado, USA. The main objective was to determine the percentage of adult striped skunks that were marked after consuming placebo oral rabies vaccine (ORV) baits containing 100 mg of an experimental microencapsulated (coated microparticle) tetracycline hydrochloride biomarker. Biomarkers were identified in the canine teeth and mandibles of five of five skunks that consumed an ORV bait. A second objective was to determine if …