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Full-Text Articles in Physical Sciences and Mathematics
Tryptophan-Like Fluorescence And Non-Point Source Pollution In Karst Basins, Inner Bluegrass Region, Kentucky, Ryan Dapkus
Tryptophan-Like Fluorescence And Non-Point Source Pollution In Karst Basins, Inner Bluegrass Region, Kentucky, Ryan Dapkus
Theses and Dissertations--Earth and Environmental Sciences
Typically, the degree of fecal contamination of water is assessed by the concentration of thermotolerant coliforms, like E. coli. This method is time-consuming, taking > 18 hours between the start of incubation and subsequent enumeration. The current study examines the utility of monitoring tryptophan-like fluorescence (TLF) using in-situ fluorometers as a proxy for fecal contamination, particularly E. coli. Relative timing of discharge peaks and TLF peaks is considered, as well as differences in E. coli concentrations and major anion concentrations between two different basins that were monitored weekly. Results indicate that TLF is somewhat useful in the prediction of …
Locating Hot Spots Of Fecal Pollution In An Urban Watershed Of Central Kentucky Using Bacteroides 16s Rrna Markers, Tricia L. Coakley
Locating Hot Spots Of Fecal Pollution In An Urban Watershed Of Central Kentucky Using Bacteroides 16s Rrna Markers, Tricia L. Coakley
Theses and Dissertations--Earth and Environmental Sciences
The field of molecular fecal source tracking in the water environment has developed rapidly since the first PCR assays for general and host-‐specific Bacteroides 16s rRNA markers were published. Numerous host-‐specific molecular markers and PCR assays have been developed, adding greater specificity, sensitivity and quantitative methods to the array of options. The public demand for readying methods for transfer to the commercial lab, so that they may be used to generate data for public utilities, citizen action groups and regulatory agencies, has fueled the development of an entire new research community. These methods, however plentiful, have not found community agreement …