Physical Sciences and Mathematics Commons^™

Past, Present, And Future Perspectives Of Environmental Dna (Edna) Metabarcoding: A Systematic Review In Methods, Monitoring, And Applications Of Global Edna, Krista M. Ruppert, Richard J. Kline, Md Saydur Rahman

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity wherein samples are taken from the environment via water, sediment or air from which DNA is extracted, and then amplified using general or universal primers in polymerase chain reaction and sequenced using next-generation sequencing to generate thousands to millions of reads. From this data, species presence can be determined, and overall biodiversity assessed. It is an interdisciplinary method that brings together traditional fieldbased ecology with in-depth molecular methods and advanced computational tools. As an emerging monitoring method, there are many pitfalls and roadblocks to be considered and avoided, but …