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Full-Text Articles in Physical Sciences and Mathematics
Nmr Analysis Of The Dynamic Exchange Of The Ns2b Cofactor Between Open And Closed Conformations Of The West Nile Virus Ns2b-Ns3 Protease, X Su, Kiyoshi Ozawa, R Qi, S G. Vasudevan, S P. Lim, G Otting
Nmr Analysis Of The Dynamic Exchange Of The Ns2b Cofactor Between Open And Closed Conformations Of The West Nile Virus Ns2b-Ns3 Protease, X Su, Kiyoshi Ozawa, R Qi, S G. Vasudevan, S P. Lim, G Otting
Faculty of Science - Papers (Archive)
Background: The two-component NS2B-NS3 proteases of West Nile and dengue viruses are essential for viral replication and established targets for drug development. In all crystal structures of the proteases to date, the NS2B cofactor is located far from the substrate binding site (open conformation) in the absence of inhibitor and lining the substrate binding site (closed conformation) in the presence of an inhibitor. Methods: In this work, nuclear magnetic resonance (NMR) spectroscopy of isotope and spin-labeled samples of the West Nile virus protease was used to investigate the occurrence of equilibria between open and closed conformations in solution. Findings: In …
Development And Application Of A System For The Analysis Of Atmospheric, Water And Sediment Nitrogen And Carbon, Ann Stavert, Stephen R. Wilson, Dianne F. Jolley
Development And Application Of A System For The Analysis Of Atmospheric, Water And Sediment Nitrogen And Carbon, Ann Stavert, Stephen R. Wilson, Dianne F. Jolley
Faculty of Science - Papers (Archive)
Eutrophication and climate change, key environmental concerns, are both linked to the carbon and nitrogen cycles hence the improved understanding of these cycles is essential. Currently, there is no system that simultaneously measures the fluxes of the three key gas phase products of nitrogen and carbon cycling (CO2, CH4 and N2O) in submerged ecosystems with hourly time resolution. A “Lake-in-a-box” (mesocosm) was developed in the laboratory which allowed the monitoring of key components of the carbon and nitrogen cycles within the air, water and sediments. The approach is automated, simple and time efficient and novel in its ability to examine …