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Full-Text Articles in Physical Sciences and Mathematics
In-Capillary Reduction And Digestion Of Proteins At Nano-Liter Volume Using Capillary Electrophoresis, Thasneen Naina Nazeema
In-Capillary Reduction And Digestion Of Proteins At Nano-Liter Volume Using Capillary Electrophoresis, Thasneen Naina Nazeema
Electronic Thesis and Dissertation Repository
To analyze the proteins present in biological samples several major steps are involved. One of the crucial steps in proteomics has been sample preparation. Prior to mass spectral analysis proteins containing disulfide bonds must be reduced which is followed by digestion. In this work, a novel way of performing protein reduction and digestion at nano-liter volume using capillary electrophoresis is presented. In this work, zone passing CE technique was utilized to analyze the reduction products in CE. After the successful in-capillary reduction, digestion of protein was also performed. A pH mediated stacking was used to bring the reduced protein and …
Analysis Of Adp-Ribosylation And Protein Sample Preparation By Capillary Electrophoresis, Ming Huang
Analysis Of Adp-Ribosylation And Protein Sample Preparation By Capillary Electrophoresis, Ming Huang
Electronic Thesis and Dissertation Repository
ADP-ribosylation is one of many biologically important protein post-translational modifications (PTMs) that received considerable research interest in the past decade. The first part of this work presents the analysis of ADP-ribosylation, with special focus on the catalytic domain of an enzyme ARTD-10. The application of capillary electrophoresis (CE) is proposed in this work to separate the in vitro enzymatic mono-ADP-ribosylated ARTD-10 catalytic domain and its unmodified form. Another exciting aspect of CE application with growing interest is the sample preparation. The effectiveness and efficiency for in-vial reaction, in-capillary mixed reaction and on-capillary-tip reaction by performing methyl esterification on phophopeptides were …
Migration Behaviour Of Discontinuous Buffers In Capillary Electrophoresis During Protein Enrichment, Ting Li
Migration Behaviour Of Discontinuous Buffers In Capillary Electrophoresis During Protein Enrichment, Ting Li
Electronic Thesis and Dissertation Repository
To enrich protein at sub-microliter sample volumes, our group has developed a discontinuous buffer system which consists of an acid and a base inside a capillary. Upon voltage application, the protein moves towards and stacks at the pH junction. Prior to this work, we only focused on the protein enrichment and have not investigated the migration behaviour of various ions in this system. However, it is important to understand the mechanism of the migration of these ions, because the pH junction formation and further the protein enrichment are based on it. In this work, the ion migration patterns at the …
Miniaturized Fractionation And Purification Of Analytes Of Interest In Complex Mixtures: Protein Enrichment And Purification With Capillary Electrophoresis And Identification Of Pesticide Chemicals In Bio-Oils, Christina J. Booker
Electronic Thesis and Dissertation Repository
The ability to isolate and detect an analyte of interest is vital for many different facets of life today, from disease detection to environmental safety. The goal of the method development discussed within this thesis is to isolate and identify unique analytes from complex matrices and to do so in a scaled down approach. Removal of an analyte from a diverse background of molecules is often required for successful detection and determination of analyte properties. As well, many samples are only available at low volumes and at trace levels so miniaturized techniques that only require small sample volumes are designed …