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Physical Sciences and Mathematics Commons™
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Full-Text Articles in Physical Sciences and Mathematics
Tracking Real-Time Nanoparticle Positions And Measuring Three-Dimensional Solution Flow With A Four-Focus Confocal Microscope, James Andrew Germann
Tracking Real-Time Nanoparticle Positions And Measuring Three-Dimensional Solution Flow With A Four-Focus Confocal Microscope, James Andrew Germann
Doctoral Dissertations
This dissertation presents the development of instrumentation for measuring the position of a single emitter within the sample volume of a confocal fluorescence microscope with sub-diffraction limited precision in three dimensions together with applications for determining solution flow and for tracking a fluorescent nanoparticle as it undergoes Brownian diffusion. The localization method is based on comparing photon counts from alternating excitation of the emitter by four laser beams, which are focused at slightly offset positions in a tetrahedral pattern within the confocal volume. Two experimental set-ups are constructed. In the first, the four beams are from a femtosecond laser, which …
A Study Of The Optical And Negative Ion Properties Of Selected Chiral Molecules, Jason Michael Lambert
A Study Of The Optical And Negative Ion Properties Of Selected Chiral Molecules, Jason Michael Lambert
Doctoral Dissertations
Chirality is subtle geometric property where objects lack reflection plane symmetry. In this thesis I study three chiral molecules using a combination of experimental and theoretical methods to elucidate the relationships between conformation freedom, solvent choice, and temperature. The importance of nuclear motion when predicting the optical rotation is explored. For carvone, corrections with each nuclear mode coordinate is important. Predictions of the ORD have the incorrect sign without the inclusion of vibrational corrections. For the case of two newly synthesized amino acid derivatives the vibrational corrections did not correct the sign of the calculated ORD to bring it into …
Three-Dimensional Electrokinetic Trapping Of A Single Fluorescent Nanoparticle In Solution, Jason Keith King
Three-Dimensional Electrokinetic Trapping Of A Single Fluorescent Nanoparticle In Solution, Jason Keith King
Doctoral Dissertations
This dissertation presents the development of an instrument for effectively trapping a single fluorescent nanoparticle that is freely diffusing in solution in all three dimensions. The instrument is expected to have applications for studies of single nanoparticles or molecules for which prolonged observations are required, but without immobilization or proximity to a surface, which may alter behavior. The trapping technique depends on rapid three-dimensional position measurements of the nanoparticle with sub-micron precision, which are used for real-time control of induced electrokinetic motion, so as to counteract Brownian motion. While anti-Brownian electrokinetic trapping experiments in one and two dimensions have previously …