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Electrophoretic Studies Of Ion Adsorption To Sarcoplasmic Reticulum And Phosphatidylcholine Membranes, Andreas Schilling

Dissertations and Theses

In this study, electrophoretic mobilities of native and two types of trypsin digested sarcoplasmic reticulum vesicles have been determined by microelectrophoresis using a Doppler Electrophoretic Light Scattering Analyzer to investigate the influence of hydrodynamic drag, caused by the Ca2⁺, Mg2⁺ -ATPase protruding from the surface of native sarcoplasmic reticulum vesicles. After the prolonged digestion (protein:trypsin ratio of 20 for 3 hours at 25°C), the ATPase was cleaved and removed from the sarcoplasmic reticulum membrane as shown with SDS gel electrophoresis and an ATPase activity assay. Ionic strength and pH dependence of mobility showed a nearly pH independent …