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Full-Text Articles in Physical Sciences and Mathematics
Characterization Of Extracellular Vesicles Derived From Mesenchymal Stromal Cells By Surface-Enhanced Raman Spectroscopy., Nina M Ćulum, Tyler T Cooper, Gillian I Bell, David A Hess, François Lagugné-Labarthet
Characterization Of Extracellular Vesicles Derived From Mesenchymal Stromal Cells By Surface-Enhanced Raman Spectroscopy., Nina M Ćulum, Tyler T Cooper, Gillian I Bell, David A Hess, François Lagugné-Labarthet
Chemistry Publications
Extracellular vesicles (EVs) are secreted by all cells into bodily fluids and play an important role in intercellular communication through the transfer of proteins and RNA. There is evidence that EVs specifically released from mesenchymal stromal cells (MSCs) are potent cell-free regenerative agents. However, for MSC EVs to be used in therapeutic practices, there must be a standardized and reproducible method for their characterization. The detection and characterization of EVs are a challenge due to their nanoscale size as well as their molecular heterogeneity. To address this challenge, we have fabricated gold nanohole arrays of varying sizes and shapes by …
Characterization Of Extracellular Vesicles Derived From Mesenchymal Stromal Cells By Surface-Enhanced Raman Spectroscopy., Nina M Ćulum, Tyler T Cooper, Gillian I Bell, David A Hess, François Lagugné-Labarthet
Characterization Of Extracellular Vesicles Derived From Mesenchymal Stromal Cells By Surface-Enhanced Raman Spectroscopy., Nina M Ćulum, Tyler T Cooper, Gillian I Bell, David A Hess, François Lagugné-Labarthet
Chemistry Publications
Extracellular vesicles (EVs) are secreted by all cells into bodily fluids and play an important role in intercellular communication through the transfer of proteins and RNA. There is evidence that EVs specifically released from mesenchymal stromal cells (MSCs) are potent cell-free regenerative agents. However, for MSC EVs to be used in therapeutic practices, there must be a standardized and reproducible method for their characterization. The detection and characterization of EVs are a challenge due to their nanoscale size as well as their molecular heterogeneity. To address this challenge, we have fabricated gold nanohole arrays of varying sizes and shapes by …
Characterization Of Extracellular Vesicles Derived From Mesenchymal Stromal Cells By Surface-Enhanced Raman Spectroscopy., Nina M. Culum, Tyler T. Cooper, Gillian I. Bell, David A. Hess, François Lagugné-Labarthet
Characterization Of Extracellular Vesicles Derived From Mesenchymal Stromal Cells By Surface-Enhanced Raman Spectroscopy., Nina M. Culum, Tyler T. Cooper, Gillian I. Bell, David A. Hess, François Lagugné-Labarthet
Chemistry Publications
Extracellular vesicles (EVs) are secreted by all cells into bodily fluids and play an important role in intercellular communication through the transfer of proteins and RNA. There is evidence that EVs specifically released from mesenchymal stromal cells (MSCs) are potent cell-free regenerative agents. However, for MSC EVs to be used in therapeutic practices, there must be a standardized and reproducible method for their characterization. The detection and characterization of EVs are a challenge due to their nanoscale size as well as their molecular heterogeneity. To address this challenge, we have fabricated gold nanohole arrays of varying sizes and shapes by …
Detection Of Gold Cysteine Thiolate Complexes On Gold Nanoparticles With Time-Of-Flight Secondary Ion Mass Spectrometry, Yolanda Hedberg, Jonas Hedberg, Valentin Romanovski, Elena Romanovskaia, Heng-Yong Nie
Detection Of Gold Cysteine Thiolate Complexes On Gold Nanoparticles With Time-Of-Flight Secondary Ion Mass Spectrometry, Yolanda Hedberg, Jonas Hedberg, Valentin Romanovski, Elena Romanovskaia, Heng-Yong Nie
Chemistry Publications
Gold (Au) nanoparticles (NPs) are widely used in nanomedical applications as a carrier for molecules designed for different functionalities. Previous findings suggested that biological molecules, including amino acids, could contribute to the dissolution of Au NPs in physiological environments and that this phenomenon was size-dependent. We, therefore, investigated the interactions of L-cysteine with 5-nm Au NPs by means of time-of-flight secondary ion mass spectrometry (ToF-SIMS). This was achieved by loading Au NPs on a clean aluminum (Al) foil and immersing it in an aqueous solution containing L-cysteine. Upon rinsing off the excessive cysteine molecules, ToF-SIMS confirmed the formation of gold …
Au Nanostructured Surfaces For Electrochemical And Localized Surface Plasmon Resonance-Based Monitoring Of Α-Synuclein-Small Molecule Interactions., Xin R Cheng, Gregory Q Wallace, François Lagugné-Labarthet, Kagan Kerman
Au Nanostructured Surfaces For Electrochemical And Localized Surface Plasmon Resonance-Based Monitoring Of Α-Synuclein-Small Molecule Interactions., Xin R Cheng, Gregory Q Wallace, François Lagugné-Labarthet, Kagan Kerman
Chemistry Publications
In this proof-of-concept study, the fabrication of novel Au nanostructured indium tin oxide (Au-ITO) surfaces is described for the development of a dual-detection platform with electrochemical and localized surface plasmon resonance (LSPR)-based biosensing capabilities. Nanosphere lithography (NSL) was applied to fabricate Au-ITO surfaces. Oligomers of α-synuclein (αS) were covalently immobilized to determine the electrochemical and LSPR characteristics of the protein. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were performed using the redox probe [Fe(CN)6](3-/4-) to detect the binding of Cu(II) ions and (-)-epigallocatechin-3-gallate (EGCG) to αS on the Au-ITO surface. Electrochemical and LSPR data were complemented by Thioflavin-T (ThT) …
Au Nanostructured Surfaces For Electrochemical And Localized Surface Plasmon Resonance-Based Monitoring Of Α-Synuclein-Small Molecule Interactions., Xin R Cheng, Gregory Q Wallace, François Lagugné-Labarthet, Kagan Kerman
Au Nanostructured Surfaces For Electrochemical And Localized Surface Plasmon Resonance-Based Monitoring Of Α-Synuclein-Small Molecule Interactions., Xin R Cheng, Gregory Q Wallace, François Lagugné-Labarthet, Kagan Kerman
Chemistry Publications
In this proof-of-concept study, the fabrication of novel Au nanostructured indium tin oxide (Au-ITO) surfaces is described for the development of a dual-detection platform with electrochemical and localized surface plasmon resonance (LSPR)-based biosensing capabilities. Nanosphere lithography (NSL) was applied to fabricate Au-ITO surfaces. Oligomers of α-synuclein (αS) were covalently immobilized to determine the electrochemical and LSPR characteristics of the protein. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were performed using the redox probe [Fe(CN)6](3-/4-) to detect the binding of Cu(II) ions and (-)-epigallocatechin-3-gallate (EGCG) to αS on the Au-ITO surface. Electrochemical and LSPR data were complemented by Thioflavin-T (ThT) …