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Full-Text Articles in Physical Sciences and Mathematics
Development Of Map/Reduce Based Microarray Analysis Tools, Guangyu Yang
Development Of Map/Reduce Based Microarray Analysis Tools, Guangyu Yang
All Theses
High density oligonucleotide array (microarray) from the Affymetrix GeneChip¨ system has been widely used for the measurements of gene expressions. Currently, public data repositories, such as Gene Expression Omnibus (GEO) of the National Center for Biotechnology Information (NCBI), have accumulated very large amount of microarray data. For example, there are 84389 human and 9654 Arabidopsis microarray experiments in GEO database. Efficiently integrative analysis large amount of microarray data will provide more knowledge about the biological systems. Traditional microarray analysis tools all implemented sequential algorithms and can only be run on single processor. They are not able to handle very large …
Comparison Of Rna Quality From Stressed And Unstressed Recombinant Escherichia Coli, Mary Alice Salazar
Comparison Of Rna Quality From Stressed And Unstressed Recombinant Escherichia Coli, Mary Alice Salazar
All Theses
High quality, intact RNA is required for DNA microarray studies, cloning, and reverse transcriptase polymerase chain reaction (rt-PCR) analysis. There are several analytical methods used to assess the RNA quality. The RNA Integrity Number (RIN) from the Agilent Bioanalyzer is one quality control assay used to evaluate RNA. For recombinant Escherichia coli cultured under stressful conditions the RNA profiles obtained using the Agilent Bioanalyzer indicate RNA degradation; however, RNA obtained and purified in parallel from unstressed recombinant cultures indicate acceptable RNA values without significant degradation. We proposed that for stressed E. coli the RIN value is not necessarily indicative of …