Physical Sciences and Mathematics Commons^™

Folate-Peg-Histidine For Use In Detection, Capture, And Analysis Of Fr+ Cells, Sean Dwyer

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Folate receptor alpha is a membrane-bound protein displaying high affinity for folic acid. This receptor serves as a receptor-mediated transport system of folic acid into cancer and cells associated with inflammation (e.g., macrophages). Folate is a basic component of cell metabolism in both the synthesis of DNA and proteins, which is hypothesized to be necessary for maintaining adequate processes during increased metabolic demand. Interestingly, most normal cells in the body have little or no high affinity folate receptor alpha. Based on these attributes, folate based drug delivery, imaging systems, and diagnostic systems are in several stages of development worldwide. To …

Synthesis Of A Folate Conjugated Fluorescent Imaging Dye, Daniel Bonner

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In recent years methods of selective targeting of pathologic cells have become increasingly important for both detection and treatment of various cancers. The use of folic acid as a selective therapeutic and targeting agent for cancer cells is becoming ever more significant. This is due to the well characterized up regulation of folate receptors for cancer cells.

In this experiment we focus on the development of a new folate conjugate dye by using a small fluorescent molecule known as Oregon Green 514 for fluorescent imaging of cancer cells. To obtain this objective we I inked folate with Oregon green 514 …

Analytical Evaluation Of Imaging Agents, Vineela Chowdary Talluri

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The folate receptor has achieved great importance for use in targeted drug delivery and in vitro and in vivo diagnostic assays. The folate receptor (FR) is over expressed on several cancer cell types and activated macrophages, but exists in relatively low abundance on the surface of non-cancerous tissue and non inflammatory cells. This unique attribute is the hallmark of folate – targeted methodologies allowing the diagnostic agent and/or drug to be ferried to the specific disease sites while preventing “off target” effects in normal tissues. Although several folate-based dyes have been reported, simple and detailed synthetic and purification methods for …