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Analysis Of Adp-Ribosylation And Protein Sample Preparation By Capillary Electrophoresis, Ming Huang
Analysis Of Adp-Ribosylation And Protein Sample Preparation By Capillary Electrophoresis, Ming Huang
Electronic Thesis and Dissertation Repository
ADP-ribosylation is one of many biologically important protein post-translational modifications (PTMs) that received considerable research interest in the past decade. The first part of this work presents the analysis of ADP-ribosylation, with special focus on the catalytic domain of an enzyme ARTD-10. The application of capillary electrophoresis (CE) is proposed in this work to separate the in vitro enzymatic mono-ADP-ribosylated ARTD-10 catalytic domain and its unmodified form. Another exciting aspect of CE application with growing interest is the sample preparation. The effectiveness and efficiency for in-vial reaction, in-capillary mixed reaction and on-capillary-tip reaction by performing methyl esterification on phophopeptides were …
Syntheses, Hydrogen Bonding, And Metal Coordination Studies Of New Ferrocene Peptide Conjugates, Craig D. Colquhoun
Syntheses, Hydrogen Bonding, And Metal Coordination Studies Of New Ferrocene Peptide Conjugates, Craig D. Colquhoun
Electronic Thesis and Dissertation Repository
The main goal of the research was the synthesis of new ferrocene peptideconjugates, to study the role of hydrogen bonding interactions, and the coordinationproperties of these systems. Ferrocene peptide conjugates have been extensivelystudied as models of protein secondary structures. Of particular interest weredisubstituted ferrocene derivatives such as ferrocene dicarboxylic acid, which have beenshown to be important in the design of peptide mimics. The inter-ring spacing betweentwo cyclopentadienyl rings in ferrocene has shown to be appropriate for forminghydrogen bonds between conjugated peptide chains, which are attached to the twocyclopentadienyl rings. Inter-ring spacing has proven to be of great importance for theformation …