Physical Sciences and Mathematics Commons^™

Slope Stability Analysis Of A Lake Michigan Coastal Bluff, Gregory Carlton Young

Masters Theses

Bluff failure along the Lake Michigan coast can be produced primarily by increased pore pressure from perched water tables above lacustrine clay deposits. To test this theory, a site in Allegan County, Michigan with alternating layers of sand, clay and glacial till was chosen for bluff failure monitoring. Historically, the site experiences sporadic massive failures as opposed to neighboring sites that show more regular and uniform displacements. Four pole and cable monitoring lines were measured bi-weekly from December 2001 to October 2003. Lake levels were low and no erosion of material at the base of the slope occurred. Slumps above …

Synthesis And Characterization Of A Recombinant Thrombin Inhibitor, Joel Lwande

Masters Theses

The final step of the blood coagulation cascade is catalyzed by thrombin, a serine protease. Like most enzyme activities, thrombin activity encounters inhibition from both direct and indirect inhibitors. A new thrombin inhibitor peptide named theromin was recently isolated from the leech Theromyzon tessulatum, and it is the most potent thrombin inhibitor isolated to date. Theromin is made up of 67 amino acid residues, out of which 16 are cysteines engaged in 8-disulfide bridges.

We synthesized the theromin gene and expressed the protein in Eschericia coli. We purified the recombinant protein and carried out thrombin inhibition assays that …

Metal Ion Imprinted Polymers For Biomolecular Recognition, Len A. Harris

Masters Theses

Protein recognition by HPLC, based on histidine residues, was achieved by incorporating a copper chelating ligand covalently bonded to a polystyrene/divinyl benzene substrate, into column packing. The metal ion was bonded to the substrate in two ways: randomly, where the copper was bound to the ligand after the substrate polymerization and imprinted, where the copper was bound to the ligand before the substrate polymerization. The effect of this templating was measured by protein separation parameters of the resulting HPLC chromatographs. The hypothesis of this thesis is the imprinted substrate will have a higher copper content, and with coppers affinity toward …

Resonant And Non-Resonant Inelastic X-Ray Scattering Of Copper Phthalocyanine, Michelle Deleene Tuel-Benckendorf

Masters Theses

With the ever-changing demands of technology and the desire to use new materials to replace the old, researchers have the job to not only find alternative materials, but also understand their properties. There continues to be extensive research in the properties of organic semiconductors, for use in many microelectronic applications. There is very little known about the excitation properties of these materials. For my research, I have chosen to study the electronic excitations of copper phthalocyanine (CuPc), one specific organic semiconductor. To do this, we used resonant and non-resonant inelastic x-ray scattering to measure the evolution of excitations as a …