Physical Sciences and Mathematics Commons^™

Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed

Masters Theses & Specialist Projects

The global concern regarding the presence of compounds with estrogenic properties in the environment has grown significantly. Endocrine-disrupting compounds (EDCs) are environmental pollutants that disrupt the endocrine systems of both wildlife and humans. Estrogens, with their potent estrogenic effects, are particularly noteworthy among the various substances with endocrine-disrupting properties. They are of concern due to their prevalence in the environment, especially due to dairy waste contamination. These lipophilic compounds can accumulate in livestock fat tissues, potentially entering the human food chain. Sulfated forms of estrone and β- estriol, which exhibit a higher degree of saturation than their non-conjugated counterparts, have …

Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor

Masters Theses & Specialist Projects

Bovine mastitis is caused by a wide range of pathogens which results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the innate immune system and act as the first line of defense against mastitis in bovine. β-defensins (~6- 10kDa) are antimicrobial peptides that contain about 28 to 67 amino acids, the presence of six cysteine residues results in their three disulfide bonds and a β-sheet structure. A few β- defensins have been identified in cows based on the site of occurrence and time of expression in tissue, but not in milk. A …

Liquid Chromatography With Tandem Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Symone T. Whalin

Masters Theses & Specialist Projects

Bovine mastitis, caused by a wide array of pathogens, results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the bovine’s innate immune system and act as the first line of defense against mastitis. Only foundational research has been done on β-defensins’ ability to treat and prevent mastitis. There have been no analytical methods reported in the literature for analyzing β-defensins in bovine milk. This research aims to create an analytical approach to determine β-defensins in bovine milk. It is challenging to determine an analyte in a complex sample matrix, and milk is …

Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi

Masters Theses & Specialist Projects

Antibiotics are used as prophylactic agents to promote growth and for treating infections in animals. However, the irrational use of antibiotics in livestock management is a significant cause of the development of antibioticresistant genes in the environment. Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 of these people are estimated to die because of antibiotic resistance. New drugs are continually coming into the market but are at the risk of developing resistance. Thus, there is a need for the development of analytical methods which can be used to monitor …

Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie

Masters Theses & Specialist Projects

While substrate-borne vibrations are utilized by different reptile species, true conspecific communication via biotremors has not yet been demonstrated in reptiles. This study follows a preliminary report that the veiled chameleon (Chamaeleo calyptratus) could produce biotremors in communicative contexts. I tested chameleon behavioral sensitivity to vibrations by placing them on a dowel attached to a shaker emitting vibrations of 25, 50, 150, 300, and 600 Hz and then measured their changes in velocity before and after the stimulus. I then paired chameleons in various social contexts [anthropogenic disturbance (human disruption of animal); dominance (malemale; female-female C. calyptratus); courtship (male-female C. …

An Evaluation Of Deterrent Methods Utilized To Prevent Crop Raiding By African Elephants (Loxodonta Africana) In The Kasigau Wildlife Corridor, Kenya, Rebecca Lynn Von Hagen

Masters Theses & Specialist Projects

Escalating human elephant conflict (HEC) continues to be a contributing factor

towards elephant decline, and crop raiding is the most common form of negative

human-elephant interactions. For communities that cannot reverse or prevent crop

raiding, it is necessary to contain HEC events through deterrent measures. Few

deterrent measures exist that combine practicality and affordability while also

preventing habituation by elephants. This project focused on comparing the efficacy of

deterrent methods to assess which was the most successful at preventing elephants

from entering crops in the farming community of Sasenyi, Kenya. In this paired-control

study, four deterrent methods were evaluated: acacia …

Detection And Quantitation Of Tetracycline Antibiotics In Agricultural Swine Wastes, Ali Jamal Abdulrheem

Masters Theses & Specialist Projects

The spread of tetracyclines through agricultural systems is causing the present bacteria to develop antibiotic resistance. The spread of this bacteria, as well as the tetracycline antibiotics in the environment is dangerous because these antibiotics pose health hazards for humans. The overuse of antibiotics, which are added to livestock feed, results in the antibiotics being released into the environment via animal feces. In this research, we have attempted to design an analytical method to isolate antibiotics from agricultural wastes with subsequent detection using liquid chromatography and mass spectrometry (LC-MS). The antibiotics investigated in this study were tetracycline, chlortetracycline, and oxytetracycline. …

Dispersion Of Ammonia From Concentrated Animal Feeding Operations, Austin D. Wardall

Masters Theses & Specialist Projects

The purpose of this research is to investigate the dispersion of ammonia (NH3) from three Concentrated Animal Feeding Operations (CAFOs) in western Kentucky, as well as to investigate the Weather Research and Forecasting – Chemistry (WRF-Chem) model’s sensitivity response to initial NH3 concentrations under both wet conditions (significant precipitation) and dry conditions (no precipitation). As expected, pollutant concentrations generally were significantly higher near their points of origin and generally declined away from the sources. Contrary to expectations, ammonia tended to rise through the planetary boundary layer (PBL) regardless of atmospheric conditions. Results showed modeled NH3 pollution levels at the surface …

The Effects Of Sulfuric Acid Deposition On The Growth And Development Of Pond Breeding Salamanders In The Genus Ambystoma, Kenneth J. Anderson

Masters Theses & Specialist Projects

In terrestrial habitats with a history of mining activity and previous or ongoing reclamation efforts, understanding the effects of acidification on the ecology of amphibians is an important part of the restoration process and the conservation of local amphibian populations. Pond-breeding amphibians spend much of their post metamorphic life history in direct contact with the soil in upland habitat adjacent to aquatic breeding sites. I reared recently metamorphosed marbled salamanders (Ambystoma opacum) to evaluate the role of soil acidity on determinants of fitness such as growth and survival. My results indicate that a substrate of pH 4 was lethal to …

Undergraduate Research Projects Help Promote Diversity In The Geosciences, De'etra Young, Shannon R. Trimboli, Rick S. Toomey, Thomas Byl

Mammoth Cave Research Symposia

No abstract provided.

Citizen Science At Mammoth Cave National Park: Integrating Research And Education, Shannon R. Trimboli

Mammoth Cave Research Symposia

No abstract provided.

Modeling Activity Of The Indiana Bat (Myotis Sodalis) At Mammoth Cave National Park Using Remotely-Sensed Descriptors Of Forest Canopy Conditions, Luke E. Dodd, N, S. Skowronski, M. B. Dickinson, L. K. Rieske, M. J. Lacki

Mammoth Cave Research Symposia

No abstract provided.

Quantitative Dye Studies To Evaluate The Spill Response System For Mammoth Cave National Park, Jetara Brown, Thomas Byl, Rick S. Toomey, Lonnie Sharpe Jr.

Mammoth Cave Research Symposia

No abstract provided.

Natural Resource Condition Assessment For Mammoth Cave National Park, Cate Webb, Chris Groves, Katie Algeo

Mammoth Cave Research Symposia

No abstract provided.

The Max Kämper Map Of Mammoth Cave: An Nps Centennial Restoration, Tres Seymour

Mammoth Cave Research Symposia

No abstract provided.

What Data Analytics Can Do For You!, Leyla Zhuhadar, J. Kirk Atkinson, Albert Meier, Ouida Meier

Mammoth Cave Research Symposia

No abstract provided.

Use Of Multiphysics Simulation To Model Environmental Conditions Associated With Bat Hibernacula Including Preliminary Indication Of Impacts On Saltpeter Vats In Mammoth Cave, Aaron Bird, Rick Olson, Rick Toomey, Aaron Addison, Rachel Bosch

Mammoth Cave Research Symposia

No abstract provided.

The Effect Of Faulting On Past And Present Hydrogeology In Long Cave, Mammoth Cave National Park, Rick Olson, Rick S. Toomey

Mammoth Cave Research Symposia

No abstract provided.

An Overview Of The Reverse Flow Patterns Of River Styx In Mammoth Cave, Kentucky: 2009-2012, Shannon R. Trimboli, Kim Weber, Susan Ryan, Rick S. Toomey

Mammoth Cave Research Symposia

No abstract provided.

Measurement Of Inorganic Carbon Fluxes From Large River Basins In South Central Kentucky Karst, Connor Salley, Chris Groves

Mammoth Cave Research Symposia

No abstract provided.

Tracing Carbon In Karst Environments In South-Central Kentucky To Identify Changes In Groundwater Dynamic Under Varying Land Uses, Chelsea Ballard, Jason Polk, Kegan Mcclanahan

Mammoth Cave Research Symposia

No abstract provided.

Ongoing Geographic Documentation Of The Mammoth Cave System And Related Caves, Pat Kambesis, Bob Osburn

Mammoth Cave Research Symposia

No abstract provided.

Strip Adaptive Cluster Sampling With Application To Cave Crickets, Kurt Lewis Helf, Tom Philippi, Bill Moore, Lillian Scoggins

Mammoth Cave Research Symposia

No abstract provided.

Organization And Development Of The Eyes Of Ptomaphagus Hirtus, The Troglobitic Small Carrion Beetle Of Mammoth Cave, Markus Friedrich, Jasmina Kulacic, Elke Buschbeck

Mammoth Cave Research Symposia

No abstract provided.

Evaluating The Energetic Value Of Lepidoptera Using Bomb Calorimetry, Shelby A. Fulton, Luke E. Dodd, Lynne K. Rieske

Mammoth Cave Research Symposia

No abstract provided.

Summary Of 2015 Winter Bat Monitoring At Mammoth Cave National Park, Laura Shultz, Chris Clark, Rick Toomey, Shannon Trimboli

Mammoth Cave Research Symposia

No abstract provided.

The Activity Of Myotis Sodalis And Myotis Septentrionalis Changes On The Landscape Of Mammoth Cave National Park Following The Arrival Of White-Nose Syndrome, Rachael Griffitts, Luke E. Dodd, Michael J. Lacki

Mammoth Cave Research Symposia

No abstract provided.

Video Presentation: Monitoring Cave Organisms, Cumberland Piedmont Inventory And Monitoring Network, Kurt Helf, Steven Thomas, Michael Durham

Mammoth Cave Research Symposia

No abstract provided.

Conducting A Biological Inventory Of Sloan’S Crossing Pond, Miranda Thompson, Jason A. Matthews, Christy Soldo

Mammoth Cave Research Symposia

No abstract provided.

The Effects Of Rainfall On Vernal Herbs, Janis Lemaster, Albert Meier

Mammoth Cave Research Symposia

No abstract provided.